Genetically engineered pigs as models for human disease

In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni

(2017) Eunji Kim et al.   Nature Communications

Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression

(2017) Tatiana Flisikowska et al.   Scientific Reports

Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene

(2017) Ki-Eun Park et al.   Scientific Reports

Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes

(2016) Van Trung Chu et al.   BMC BIOTECHNOLOGY

Using the mouse to model human disease: increasing validity and reproducibility

(2016) Monica J. Justice et al.   Disease Models & Mechanisms

Expression of the Alzheimer’s Disease Mutations AβPP695sw and PSEN1M146I in Double-Transgenic Göttingen Minipigs

(2016) Jannik E. Jakobsen et al.   JOURNAL OF ALZHEIMERS DISEASE

Neuroimaging of a minipig model of Huntington's disease: Feasibility of volumetric, diffusion-weighted and spectroscopic assessments

(2016) Robin Schubert et al.   JOURNAL OF NEUROSCIENCE METHODS

Defeating Alzheimer's disease and other dementias: a priority for European science and society

(2016) Bengt Winblad et al.   LANCET NEUROLOGY

Generation of an inducible colon-specific Cre enzyme mouse line for colon cancer research

(2016) Paul W. Tetteh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In vitro fertilization in pigs: New molecules and protocols to consider in the forthcoming years

(2016) Raquel Romar et al.   THERIOGENOLOGY

Generation and characterization of RAG2 knockout pigs as animal model for severe combined immunodeficiency

(2016) Shunichi Suzuki et al.   VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY

A porcine model of osteosarcoma

(2016) A Saalfrank et al.   Oncogenesis

Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing

(2016) Yuichiro Miyaoka et al.   Scientific Reports

Rosa26-targeted sheep gene knock-in via CRISPR-Cas9 system

(2016) Mingming Wu et al.   Scientific Reports

Progressive muscle proteome changes in a clinically relevant pig model of Duchenne muscular dystrophy

(2016) Thomas Fröhlich et al.   Scientific Reports

Diabetes with poor glycaemic control does not promote atherosclerosis in genetically modified hypercholesterolaemic minipigs

(2015) Rozh H. Al-Mashhadi et al.   DIABETOLOGIA

Animal models of Duchenne muscular dystrophy: from basic mechanisms to gene therapy

(2015) J. W. McGreevy et al.   Disease Models & Mechanisms

Efficient Generation of Gene-Modified Pigs Harboring Precise Orthologous Human Mutation via CRISPR/Cas9-Induced Homology-Directed Repair in Zygotes

(2015) Xiaoyang Zhou et al.   HUMAN MUTATION

Incretin actions and consequences of incretin-based therapies: lessons from complementary animal models

(2015) Simone Renner et al.   JOURNAL OF PATHOLOGY

Effects of the glucagon-like peptide-1 receptor agonist liraglutide in juvenile transgenic pigs modeling a pre-diabetic condition

(2015) Elisabeth Streckel et al.   Journal of Translational Medicine

Origins of Cystic Fibrosis Lung Disease

(2015) David A. Stoltz et al.   NEW ENGLAND JOURNAL OF MEDICINE

Development of an intein-mediated split–Cas9 system for gene therapy

(2015) Dong-Jiunn Jeffery Truong et al.   NUCLEIC ACIDS RESEARCH

A Genetic Porcine Model of Cancer

(2015) Lawrence B. Schook et al.   PLoS One

Viable pigs with a conditionally-activated oncogenic KRAS mutation

(2015) Shun Li et al.   TRANSGENIC RESEARCH

Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA

(2015) Yong Wang et al.   Scientific Reports

Liraglutide Improves Pancreatic Beta Cell Mass and Function in Alloxan-Induced Diabetic Mice

(2015) Kanako Tamura et al.   PLoS One

A new rolling culture-basedin vitrofertilization system capable of reducing polyspermy in porcine oocytes

(2014) Hideki Kitaji et al.   ANIMAL SCIENCE JOURNAL

Use of the CRISPR/Cas9 System to Produce Genetically Engineered Pigs from In Vitro-Derived Oocytes and Embryos1

(2014) Kristin M. Whitworth et al.   BIOLOGY OF REPRODUCTION

Early-Onset Familial Alzheimer's Disease (EOFAD)

(2014) Liyong Wu et al.   CANADIAN JOURNAL OF NEUROLOGICAL SCIENCES

One-step generation of knockout pigs by zygote injection of CRISPR/Cas system

(2014) Tang Hai et al.   CELL RESEARCH

Increasing Efficiency in Production of Cloned Piglets

(2014) Henrik Callesen et al.   Cellular Reprogramming

Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012

(2014) Jacques Ferlay et al.   INTERNATIONAL JOURNAL OF CANCER

Development and translational imaging of a TP53 porcine tumorigenesis model

(2014) Jessica C. Sieren et al.   JOURNAL OF CLINICAL INVESTIGATION

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

A next-generation dual-recombinase system for time- and host-specific targeting of pancreatic cancer

(2014) Nina Schönhuber et al.   NATURE MEDICINE

Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons

(2014) Zoltán Ivics et al.   Nature Protocols

Production of Pigs Expressing a Transgene under the Control of a Tetracycline-Inducible System

(2014) Yong-Xun Jin et al.   PLoS One

Diffuse Glomerular Nodular Lesions in Diabetic Pigs Carrying a Dominant-Negative Mutant Hepatocyte Nuclear Factor 1-Alpha, an Inheritant Diabetic Gene in Humans

(2014) Satoshi Hara et al.   PLoS One

Targeted Disruption of LDLR Causes Hypercholesterolemia and Atherosclerosis in Yucatan Miniature Pigs

(2014) Bryan T. Davis et al.   PLoS One

Dual Fluorescent Reporter Pig for Cre Recombination: Transgene Placement at the ROSA26 Locus

(2014) Shun Li et al.   PLoS One

Generation of Recombination Activating Gene-1-Deficient Neonatal Piglets: A Model of T and B Cell Deficient Severe Combined Immune Deficiency

(2014) Tetsuya Ito et al.   PLoS One

Patient-Derived Xenograft Models: An Emerging Platform for Translational Cancer Research

(2014) M. Hidalgo et al.   Cancer Discovery

Animal Models in Osteosarcoma

(2014) Maria V. Guijarro et al.   Frontiers in Oncology

Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set

(2013) Mayuko Kurome et al.   BMC BIOTECHNOLOGY

A mouse model for HBV immunotolerance and immunotherapy

(2013) Dan Yang et al.   Cellular & Molecular Immunology

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

(2013) S. W. Cho et al.   GENOME RESEARCH

Dystrophin-deficient pigs provide new insights into the hierarchy of physiological derangements of dystrophic muscle

(2013) Nikolai Klymiuk et al.   HUMAN MOLECULAR GENETICS

Intestinal CFTR expression alleviates meconium ileus in cystic fibrosis pigs

(2013) David A. Stoltz et al.   JOURNAL OF CLINICAL INVESTIGATION

Production of Diabetic Offspring Using Cryopreserved Epididymal Sperm by In Vitro Fertilization and Intrafallopian Insemination Techniques in Transgenic Pigs

(2013) Kazuhiro UMEYAMA et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Use of pigs as a potential model for research into dietary modulation of the human gut microbiota

(2013) Sonja N. Heinritz et al.   NUTRITION RESEARCH REVIEWS

CFTR-deficient pigs display peripheral nervous system defects at birth

(2013) L. R. Reznikov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient nonmeiotic allele introgression in livestock using custom endonucleases

(2013) W. Tan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Familial Hypercholesterolemia and Atherosclerosis in Cloned Minipigs Created by DNA Transposition of a Human PCSK9 Gain-of-Function Mutant

(2013) R. H. Al-Mashhadi et al.   Science Translational Medicine

Generation of AQP2-Cre transgenic mini-pigs specifically expressing Cre recombinase in kidney collecting duct cells

(2013) Weiwei Luo et al.   TRANSGENIC RESEARCH

Production of biallelic CMP-Neu5Ac hydroxylase knock-out pigs

(2013) Deug-Nam Kwon et al.   Scientific Reports

Live pigs produced from genome edited zygotes

(2013) Simon G. Lillico et al.   Scientific Reports

Animal Models of Alzheimer Disease

(2013) F. M. LaFerla et al.   Cold Spring Harbor Perspectives in Medicine

A new era in the treatment of cystic fibrosis: correction of the underlying CFTR defect

(2013) Michael P Boyle et al.   Lancet Respiratory Medicine

Production of piglets from in vitro-produced embryos following non-surgical transfer

(2012) Koji Yoshioka et al.   ANIMAL REPRODUCTION SCIENCE

Evidence-based path to newborn screening for duchenne muscular dystrophy

(2012) Jerry R. Mendell et al.   ANNALS OF NEUROLOGY

The use of animal models in diabetes research

(2012) Aileen JF King   BRITISH JOURNAL OF PHARMACOLOGY

Changing Metabolic Signatures of Amino Acids and Lipids During the Prediabetic Period in a Pig Model With Impaired Incretin Function and Reduced  -Cell Mass

(2012) S. Renner et al.   DIABETES

Permanent Neonatal Diabetes in INSC94Y Transgenic Pigs

(2012) S. Renner et al.   DIABETES

Diagnosis and Classification of Diabetes Mellitus

(2012)   DIABETES CARE

A Porcine Model of Familial Adenomatous Polyposis

(2012) Tatiana Flisikowska et al.   GASTROENTEROLOGY

Analyses of pig genomes provide insight into porcine demography and evolution

(2012) Martien A. M. Groenen et al.   NATURE

Animal models for HIV/AIDS research

(2012) Theodora Hatziioannou et al.   NATURE REVIEWS MICROBIOLOGY

Inactivation and Inducible Oncogenic Mutation of p53 in Gene Targeted Pigs

(2012) Simon Leuchs et al.   PLoS One

Efficient TALEN-mediated gene knockout in livestock

(2012) D. F. Carlson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Pluripotent cells in farm animals: state of the art and future perspectives

(2012) Monika Nowak-Imialek et al.   REPRODUCTION FERTILITY AND DEVELOPMENT

Domestic Animal Models for Biomedical Research

(2012) A Bähr et al.   REPRODUCTION IN DOMESTIC ANIMALS

Generation of minipigs with targeted transgene insertion by recombinase-mediated cassette exchange (RMCE) and somatic cell nuclear transfer (SCNT)

(2012) Jannik Ejnar Jakobsen et al.   TRANSGENIC RESEARCH

Molecular Genetics of Colorectal Cancer

(2011) Eric R. Fearon   Annual Review of Pathology-Mechanisms of Disease

Sarcomas in TP53 germline mutation carriers

(2011) Simona Ognjanovic et al.   CANCER

The human glucagon-like peptide-1 analogue liraglutide preserves pancreatic beta cells via regulation of cell kinetics and suppression of oxidative and endoplasmic reticulum stress in a mouse model of diabetes

(2011) M. Shimoda et al.   DIABETOLOGIA

First inducible transgene expression in porcine large animal models

(2011) Nikolai Klymiuk et al.   FASEB JOURNAL

Characterization of a hypertriglyceridemic transgenic miniature pig model expressing human apolipoprotein CIII

(2011) Jingyuan Wei et al.   FEBS Journal

Mammalian Models of Duchenne Muscular Dystrophy: Pathological Characteristics and Therapeutic Applications

(2011) Akinori Nakamura et al.   JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY

Mouse models of cystic fibrosis: Phenotypic analysis and research applications

(2011) Martina Wilke et al.   Journal of Cystic Fibrosis

Sequential targeting of CFTR by BAC vectors generates a novel pig model of cystic fibrosis

(2011) N. Klymiuk et al.   JOURNAL OF MOLECULAR MEDICINE-JMM

Alzheimer's disease

(2011) Clive Ballard et al.   LANCET

Translational research: 4 ways to fix the clinical trial

(2011) Heidi Ledford   NATURE

A conditional knockout resource for the genome-wide study of mouse gene function

(2011) William C. Skarnes et al.   NATURE

High drug attrition rates—where are we going wrong?

(2011) Lisa Hutchinson et al.   Nature Reviews Clinical Oncology

Systemic Administration of PRO051 in Duchenne's Muscular Dystrophy

(2011) Nathalie M. Goemans et al.   NEW ENGLAND JOURNAL OF MEDICINE

Efficient Immunoglobulin Gene Disruption and Targeted Replacement in Rabbit Using Zinc Finger Nucleases

(2011) Tatiana Flisikowska et al.   PLoS One

Germline Transgenic Pigs by Sleeping Beauty Transposition in Porcine Zygotes and Targeted Integration in the Pig Genome

(2011) Wiebke Garrels et al.   PLoS One

Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases

(2011) J. Hauschild et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The  F508 Mutation Causes CFTR Misprocessing and Cystic Fibrosis-Like Disease in Pigs

(2011) L. S. Ostedgaard et al.   Science Translational Medicine

Loss of Cystic Fibrosis Transmembrane Conductance Regulator Function Produces Abnormalities in Tracheal Development in Neonatal Pigs and Young Children

(2010) David K. Meyerholz et al.   AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE

Atherosclerotic lesions in mouse and man: is it the same disease?

(2010) Jacob Fog Bentzon et al.   CURRENT OPINION IN LIPIDOLOGY

Glucose Intolerance and Reduced Proliferation of Pancreatic  -Cells in Transgenic Pigs With Impaired Glucose-Dependent Insulinotropic Polypeptide Function

(2010) S. Renner et al.   DIABETES

Preclinical PK and PD Studies on 2′-O-Methyl-phosphorothioate RNA Antisense Oligonucleotides in the mdx Mouse Model

(2010) Hans Heemskerk et al.   MOLECULAR THERAPY

Development of a mouse model for sporadic and metastatic colon tumors and its use in assessing drug treatment

(2010) K. E. Hung et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases

(2010) M. Meyer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High efficiency of BRCA1 knockout using rAAV-mediated gene targeting: developing a pig model for breast cancer

(2010) Yonglun Luo et al.   TRANSGENIC RESEARCH

Differential colorectal carcinogenesis: Molecular basis and clinical relevance

(2010) Alberto Morán   World Journal of Gastrointestinal Oncology

International osteosarcoma incidence patterns in children and adolescents, middle ages and elderly persons

(2009) Lisa Mirabello et al.   INTERNATIONAL JOURNAL OF CANCER

Hemizygous minipigs produced by random gene insertion and handmade cloning express the Alzheimer’s disease-causing dominant mutation APPsw

(2009) Peter M. Kragh et al.   TRANSGENIC RESEARCH

Dominant-negative mutant hepatocyte nuclear factor 1α induces diabetes in transgenic-cloned pigs

(2009) Kazuhiro Umeyama et al.   TRANSGENIC RESEARCH

Production of CFTR-null and CFTR-ΔF508 heterozygous pigs by adeno-associated virus–mediated gene targeting and somatic cell nuclear transfer

(2008) Christopher S. Rogers et al.   JOURNAL OF CLINICAL INVESTIGATION

Disruption of the CFTR Gene Produces a Model of Cystic Fibrosis in Newborn Pigs

(2008) C. S. Rogers et al.   SCIENCE

Diabetes models by screen for hyperglycemia in phenotype-driven ENU mouse mutagenesis projects

(2007) Bernhard Aigner et al.   AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM