Reinvigorating natural product combinatorial biosynthesis with synthetic biology

De novo production of the plant-derived alkaloid strictosidine in yeast

(2015) Stephanie Brown et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reprogramming Nonribosomal Peptide Synthetases for “Clickable” Amino Acids

(2014) Hajo Kries et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Rational Design of Modular Polyketide Synthases: Morphing the Aureothin Pathway into a Luteoreticulin Assembly Line

(2014) Yuki Sugimoto et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Metagenomic small molecule discovery methods

(2014) Zachary Charlop-Powers et al.   CURRENT OPINION IN MICROBIOLOGY

Probing the Phosphopantetheine Arm Conformations of Acyl Carrier Proteins Using Vibrational Spectroscopy

(2014) Matthew N. R. Johnson et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biosynthesis of fungal indole alkaloids

(2014) Wei Xu et al.   NATURAL PRODUCT REPORTS

Chemo- and site-selective derivatizations of natural products enabling biological studies

(2014) Omar Robles et al.   NATURAL PRODUCT REPORTS

Microbial biosynthesis of medicinally important plant secondary metabolites

(2014) Myoung Chong Song et al.   NATURAL PRODUCT REPORTS

Structure of a modular polyketide synthase

(2014) Somnath Dutta et al.   NATURE

Structural rearrangements of a polyketide synthase module during its catalytic cycle

(2014) Jonathan R. Whicher et al.   NATURE

Bioretrosynthetic construction of a didanosine biosynthetic pathway

(2014) William R Birmingham et al.   Nature Chemical Biology

De novo biosynthesis of terminal alkyne-labeled natural products

(2014) Xuejun Zhu et al.   Nature Chemical Biology

Programming biological operating systems: genome design, assembly and activation

(2014) Daniel G Gibson   NATURE METHODS

Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A

(2014) Kazuya Yamanaka et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Targeted Capture and Heterologous Expression of the Pseudoalteromonas Alterochromide Gene Cluster in Escherichia coli Represents a Promising Natural Product Exploratory Platform

(2014) Avena C. Ross et al.   ACS Synthetic Biology

Biosynthesis of Antimycins with a Reconstituted 3-Formamidosalicylate Pharmacophore in Escherichia coli

(2014) Joyce Liu et al.   ACS Synthetic Biology

Assessing the Combinatorial Potential of the RiPP Cyanobactin tru Pathway

(2014) Duane E. Ruffner et al.   ACS Synthetic Biology

Homology-Integrated CRISPR–Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae

(2014) Zehua Bao et al.   ACS Synthetic Biology

High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

(2014) Ryan E. Cobb et al.   ACS Synthetic Biology

Multiplexing of Combinatorial Chemistry in Antimycin Biosynthesis: Expansion of Molecular Diversity and Utility

(2013) Yan Yan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cyanobacterial Polyketide Synthase Docking Domains: A Tool for Engineering Natural Product Biosynthesis

(2013) Jonathan R. Whicher et al.   CHEMISTRY & BIOLOGY

DNA assembly techniques for next-generation combinatorial biosynthesis of natural products

(2013) Ryan E. Cobb et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Microbial genome mining for accelerated natural products discovery: is a renaissance in the making?

(2013) Brian O. Bachmann et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

An Efficient System for Heterologous Expression of Secondary Metabolite Genes in Aspergillus nidulans

(2013) Yi-Ming Chiang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Engineering the acyltransferase substrate specificity of assembly line polyketide synthases

(2013) B. J. Dunn et al.   Journal of the Royal Society Interface

Design, construction and characterisation of a synthetic promoter library for fine-tuned gene expression in actinomycetes

(2013) Theresa Siegl et al.   METABOLIC ENGINEERING

Recent advances in the heterologous expression of microbial natural product biosynthetic pathways

(2013) Sarah E. Ongley et al.   NATURAL PRODUCT REPORTS

Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs

(2013) Dokyun Na et al.   NATURE BIOTECHNOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)

(2013) Gabriel C. Uguru et al.   PLoS One

Expanding the Fluorine Chemistry of Living Systems Using Engineered Polyketide Synthase Pathways

(2013) M. C. Walker et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Engineered Streptomyces avermitilis Host for Heterologous Expression of Biosynthetic Gene Cluster for Secondary Metabolites

(2013) Mamoru Komatsu et al.   ACS Synthetic Biology

Poly Specific trans-Acyltransferase Machinery Revealed via Engineered Acyl-CoA Synthetases

(2012) Irina Koryakina et al.   ACS Chemical Biology

Enzyme-Directed Mutasynthesis: A Combined Experimental and Theoretical Approach to Substrate Recognition of a Polyketide Synthase

(2012) Uschi Sundermann et al.   ACS Chemical Biology

Minimally Invasive Mutagenesis Gives Rise to a Biosynthetic Polyketide Library

(2012) Susanna Kushnir et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Heterologous Expression and Manipulation of Three Tetracycline Biosynthetic Pathways

(2012) Peng Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Introduction of a Non-Natural Amino Acid into a Nonribosomal Peptide Antibiotic by Modification of Adenylation Domain Specificity

(2012) Jenny Thirlway et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Direct Cloning, Genetic Engineering, and Heterologous Expression of the Syringolin Biosynthetic Gene Cluster inE. colithrough Red/ET Recombineering

(2012) Xiaoying Bian et al.   CHEMBIOCHEM

Ribosome-independent biosynthesis of biologically active peptides: Application of synthetic biology to generate structural diversity

(2012) Tobias W. Giessen et al.   FEBS LETTERS

Sequential deletion of all the polyketide synthase and nonribosomal peptide synthetase biosynthetic gene clusters and a 900-kb subtelomeric sequence of the linear chromosome of Streptomyces coelicolor

(2012) Min Zhou et al.   FEMS MICROBIOLOGY LETTERS

Natural Products As Sources of New Drugs over the 30 Years from 1981 to 2010

(2012) David J. Newman et al.   JOURNAL OF NATURAL PRODUCTS

Biosynthetic Multitasking Facilitates Thalassospiramide Structural Diversity in Marine Bacteria

(2012) Avena C. Ross et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

2-Deoxystreptamine-containing aminoglycoside antibiotics: Recent advances in the characterization and manipulation of their biosynthetic pathways

(2012) Sung Ryeol Park et al.   NATURAL PRODUCT REPORTS

Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature

(2012) Paul G. Arnison et al.   NATURAL PRODUCT REPORTS

Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting

(2012) Jun Fu et al.   NATURE BIOTECHNOLOGY

Computational tools for the synthetic design of biochemical pathways

(2012) Marnix H. Medema et al.   NATURE REVIEWS MICROBIOLOGY

Modular control of multiple pathways using engineered orthogonal T7 polymerases

(2012) Karsten Temme et al.   NUCLEIC ACIDS RESEARCH

Reprogramming a module of the 6-deoxyerythronolide B synthase for iterative chain elongation

(2012) S. Kapur et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Modular Construction of a Functional Artificial Epothilone Polyketide Pathway

(2012) Corina Oßwald et al.   ACS Synthetic Biology

Combinatorial Biosynthesis of Cyclic Lipopeptide Antibiotics: A Model for Synthetic Biology To Accelerate the Evolution of Secondary Metabolite Biosynthetic Pathways

(2012) Richard H. Baltz   ACS Synthetic Biology

Opportunities for Synthetic Biology in Antibiotics: Expanding Glycopeptide Chemical Diversity

(2012) Maulik N. Thaker et al.   ACS Synthetic Biology

Directed Evolution of the Nonribosomal Peptide Synthetase AdmK Generates New Andrimid Derivatives In Vivo

(2011) Bradley S. Evans et al.   CHEMISTRY & BIOLOGY

Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler

(2011) Zengyi Shao et al.   Molecular BioSystems

Beyond ethylmalonyl-CoA: The functional role of crotonyl-CoAcarboxylase/reductase homologs in expanding polyketide diversity

(2011) Micheal C. Wilson et al.   NATURAL PRODUCT REPORTS

DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency

(2011) Robert J. Conrado et al.   NUCLEIC ACIDS RESEARCH

Functional analysis of environmental DNA-derived type II polyketide synthases reveals structurally diverse secondary metabolites

(2011) Z. Feng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Organization of Intracellular Reactions with Rationally Designed RNA Assemblies

(2011) C. J. Delebecque et al.   SCIENCE

Complete Biosynthesis of Erythromycin A and Designed Analogs Using E. coli as a Heterologous Host

(2010) Haoran Zhang et al.   CHEMISTRY & BIOLOGY

Gene Expression Enabling Synthetic Diversification of Natural Products: Chemogenetic Generation of Pacidamycin Analogs

(2010) Abhijeet Deb Roy et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Fluostatins Produced by the Heterologous Expression of a TAR Reassembled Environmental DNA Derived Type II PKS Gene Cluster

(2010) Zhiyang Feng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biosynthesis of the Allylmalonyl-CoA Extender Unit for the FK506 Polyketide Synthase Proceeds through a Dedicated Polyketide Synthase and Facilitates the Mutasynthesis of Analogues

(2010) SangJoon Mo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Post-PKS tailoring steps in natural product-producing actinomycetes from the perspective of combinatorial biosynthesis

(2010) Carlos Olano et al.   NATURAL PRODUCT REPORTS

Biosynthesis of polyketides by trans-AT polyketide synthases

(2010) Jörn Piel   NATURAL PRODUCT REPORTS

Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism

(2010) M. Komatsu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters

(2010) Juan Pablo Gomez-Escribano et al.   Microbial Biotechnology

The Biosynthetic Logic of Polyketide Diversity

(2009) Christian Hertweck   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

High titer production of tetracenomycins by heterologous expression of the pathway in a Streptomyces cinnamonensis industrial monensin producer strain

(2009) Chaoxuan Li et al.   METABOLIC ENGINEERING

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Synthetic protein scaffolds provide modular control over metabolic flux

(2009) John E Dueber et al.   NATURE BIOTECHNOLOGY

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Biosynthesis of the salinosporamide A polyketide synthase substrate chloroethylmalonyl-coenzyme A from S-adenosyl-L-methionine

(2009) A. S. Eustaquio et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Drug Discovery and Natural Products: End of an Era or an Endless Frontier?

(2009) J. W.-H. Li et al.   SCIENCE

Complete Reconstitution of a Highly Reducing Iterative Polyketide Synthase

(2009) S. M. Ma et al.   SCIENCE

Recent advances in the biochemistry of spinosyns

(2008) Ke-xue Huang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Metabolic engineering of microorganisms for isoprenoid production

(2008) James Kirby et al.   NATURAL PRODUCT REPORTS

Crystal Structure of the Termination Module of a Nonribosomal Peptide Synthetase

(2008) A. Tanovic et al.   SCIENCE