Direct Cloning, Genetic Engineering, and Heterologous Expression of the Syringolin Biosynthetic Gene Cluster inE. colithrough Red/ET Recombineering

Heterologous Expression and Genetic Engineering of the Tubulysin Biosynthetic Gene Cluster Using Red/ET Recombineering and Inactivation Mutagenesis

(2012) Yi Chai et al.   CHEMISTRY & BIOLOGY

Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting

(2012) Jun Fu et al.   NATURE BIOTECHNOLOGY

Enzymatic Timing and Tailoring of Macrolactamization in Syringolin Biosynthesis

(2011) William M. Wuest et al.   ORGANIC LETTERS

Syrbactin class proteasome inhibitor-induced apoptosis and autophagy occurs in association with p53 accumulation and Akt/PKB activation in neuroblastoma

(2010) Crystal R. Archer et al.   BIOCHEMICAL PHARMACOLOGY

Biosynthesis of the proteasome inhibitor syringolin A: the ureido group joining two amino acids originates from bicarbonate

(2009) Christina Ramel et al.   BMC BIOCHEMISTRY

Syringolin A Selectively Labels the 20 S Proteasome in Murine EL4 and Wild-Type and Bortezomib-Adapted Leukaemic Cell Lines

(2009) Jérôme Clerc et al.   CHEMBIOCHEM

SylC Catalyzes Ureido-Bond Formation During Biosynthesis of the Proteasome Inhibitor Syringolin A

(2009) Heidi J. Imker et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli

(2009) Bryson D Bennett et al.   Nature Chemical Biology

Synthetic and structural studies on syringolin A and B reveal critical determinants of selectivity and potency of proteasome inhibition

(2009) J. Clerc et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Assembly and Heterologous Expression of the Coumermycin A1 Gene Cluster and Production of New Derivatives by Genetic Engineering

(2008) Manuel Wolpert et al.   CHEMBIOCHEM

A plant pathogen virulence factor inhibits the eukaryotic proteasome by a novel mechanism

(2008) Michael Groll et al.   NATURE

Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition

(2008) Jun Fu et al.   NUCLEIC ACIDS RESEARCH

Heterologous Expression And Genetic Engineering of the Phenalinolactone Biosynthetic Gene Cluster by Using Red/ET Recombineering

(2007) Tina M. Binz et al.   CHEMBIOCHEM