Engineering the acyltransferase substrate specificity of assembly line polyketide synthases

Quantification ofN-acetylcysteamine activated methylmalonate incorporation into polyketide biosynthesis

(2013) Stephan Klopries et al.   Beilstein Journal of Organic Chemistry

Mechanism and Specificity of an Acyltransferase Domain from a Modular Polyketide Synthase

(2013) Briana J. Dunn et al.   BIOCHEMISTRY

Poly Specific trans-Acyltransferase Machinery Revealed via Engineered Acyl-CoA Synthetases

(2012) Irina Koryakina et al.   ACS Chemical Biology

Substrate Specificity in Ketosynthase Domains fromtrans-AT Polyketide Synthases

(2012) Matthew Jenner et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Antibiotics: A New Hope

(2012) Gerard D. Wright   CHEMISTRY & BIOLOGY

Combinatorial biosynthesis of polyketides—a perspective

(2012) Fong T Wong et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Biochemical and Structural Study of the Atypical Acyltransferase Domain from the Mycobacterial Polyketide Synthase Pks13

(2012) Fabien Bergeret et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Molecular Insights into the Biosynthesis of Guadinomine: A Type III Secretion System Inhibitor

(2012) Tracy C. Holmes et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Inter-domain movements in polyketide synthases: a molecular dynamics study

(2012) Swadha Anand et al.   Molecular BioSystems

Protein co-evolution: how do we combine bioinformatics and experimental approaches?

(2012) Inga Sandler et al.   Molecular BioSystems

The structures of type I polyketide synthases

(2012) Adrian T. Keatinge-Clay   NATURAL PRODUCT REPORTS

Reprogramming a module of the 6-deoxyerythronolide B synthase for iterative chain elongation

(2012) S. Kapur et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Discrete acyltransferases involved in polyketide biosynthesis

(2012) Ewa Maria Musiol et al.   MedChemComm

Modeling holo-ACP:DH and holo-ACP:KR complexes of modular polyketide synthases: a docking and molecular dynamics study

(2012) Swadha Anand et al.   BMC STRUCTURAL BIOLOGY

Structure and Mechanism of thetrans-Acting Acyltransferase from the Disorazole Synthase

(2011) Fong T. Wong et al.   BIOCHEMISTRY

JDet: interactive calculation and visualization of function-related conservation patterns in multiple sequence alignments and structures

(2011) T. Muth et al.   BIOINFORMATICS

Molecular dynamics simulations and drug discovery

(2011) Jacob D Durrant et al.   BMC BIOLOGY

Hot Spots for Allosteric Regulation on Protein Surfaces

(2011) Kimberly A. Reynolds et al.   CELL

Mutant Malonyl-CoA Synthetases with Altered Specificity for Polyketide Synthase Extender Unit Generation

(2011) Irina Koryakina et al.   CHEMBIOCHEM

Acyl-CoA Subunit Selectivity in the Pikromycin Polyketide Synthase PikAIV: Steady-State Kinetics and Active-Site Occupancy Analysis by FTICR-MS

(2011) Shilah A. Bonnett et al.   CHEMISTRY & BIOLOGY

Enzymatic Extender Unit Generation for In Vitro Polyketide Synthase Reactions: Structural and Func-tional Showcasing of Streptomyces coelicolor MatB

(2011) Amanda J. Hughes et al.   CHEMISTRY & BIOLOGY

Supramolecular Templating in Kirromycin Biosynthesis: The Acyltransferase KirCII Loads Ethylmalonyl-CoA Extender onto a Specific ACP of the trans-AT PKS

(2011) Ewa M. Musiol et al.   CHEMISTRY & BIOLOGY

Strategy and success for the directed evolution of enzymes

(2011) Paul A Dalby   CURRENT OPINION IN STRUCTURAL BIOLOGY

Beyond ethylmalonyl-CoA: The functional role of crotonyl-CoAcarboxylase/reductase homologs in expanding polyketide diversity

(2011) Micheal C. Wilson et al.   NATURAL PRODUCT REPORTS

Probing the interactions of an acyl carrier protein domain from the 6-deoxyerythronolide B synthase

(2011) Louise K. Charkoudian et al.   PROTEIN SCIENCE

An automated stochastic approach to the identification of the protein specificity determinants and functional subfamilies

(2010) Pavel V Mazin et al.   Algorithms for Molecular Biology

Recognition of (2S)-Aminomalonyl-Acyl Carrier Protein (ACP) and (2R)-Hydroxymalonyl-ACP by Acyltransferases in Zwittermicin A Biosynthesis

(2010) Yolande A. Chan et al.   BIOCHEMISTRY

Genome Mining Reveals trans-AT Polyketide Synthase Directed Antibiotic Biosynthesis in the Bacterial Phylum Bacteroidetes

(2010) Roberta Teta  et al.   CHEMBIOCHEM

Biosynthesis of the Allylmalonyl-CoA Extender Unit for the FK506 Polyketide Synthase Proceeds through a Dedicated Polyketide Synthase and Facilitates the Mutasynthesis of Analogues

(2010) SangJoon Mo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

An interdomain sector mediating allostery in Hsp70 molecular chaperones

(2010) Robert G Smock et al.   Molecular Systems Biology

Biosynthesis of polyketides by trans-AT polyketide synthases

(2010) Jörn Piel   NATURAL PRODUCT REPORTS

SBSPKS: structure based sequence analysis of polyketide synthases

(2010) Swadha Anand et al.   NUCLEIC ACIDS RESEARCH

Molecular recognition between ketosynthase and acyl carrier protein domains of the 6-deoxyerythronolide B synthase

(2010) S. Kapur et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Protein interactions and ligand binding: From protein subfamilies to functional specificity

(2010) A. Rausell et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Protein−Protein Recognition between Acyltransferases and Acyl Carrier Proteins in Multimodular Polyketide Synthases

(2009) Fong T. Wong et al.   BIOCHEMISTRY

Protein Sectors: Evolutionary Units of Three-Dimensional Structure

(2009) Najeeb Halabi et al.   CELL

Directed Evolution and Structural Characterization of a Simvastatin Synthase

(2009) Xue Gao et al.   CHEMISTRY & BIOLOGY

Computational tools for designing and engineering biocatalysts

(2009) Jiri Damborsky et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Directed enzyme evolution: climbing fitness peaks one amino acid at a time

(2009) Cara A Tracewell et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

The kirromycin gene cluster of Streptomyces collinus Tü 365 codes for an aspartate-α-decarboxylase, KirD, which is involved in the biosynthesis of the precursor β-alanine

(2009) Kristina J Laiple et al.   JOURNAL OF ANTIBIOTICS

Computational structure-based redesign of enzyme activity

(2009) C.-Y. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Drug Discovery and Natural Products: End of an Era or an Endless Frontier?

(2009) J. W.-H. Li et al.   SCIENCE

Protein Design by Directed Evolution

(2008) Christian Jäckel et al.   Annual Review of Biophysics

Mechanism based protein crosslinking of domains from the 6-deoxyerythronolide B synthase

(2008) Shiven Kapur et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

In Vivo and In Vitro Trans-Acylation by BryP, the Putative Bryostatin Pathway Acyltransferase Derived from an Uncultured Marine Symbiont

(2008) Nicole B. Lopanik et al.   CHEMISTRY & BIOLOGY

Molecular Analysis of the Kirromycin Biosynthetic Gene Cluster Revealed β-Alanine as Precursor of the Pyridone Moiety

(2008) Tilmann Weber et al.   CHEMISTRY & BIOLOGY

The minimized dead-end elimination criterion and its application to protein redesign in a hybrid scoring and search algorithm for computing partition functions over molecular ensembles

(2008) Ivelin Georgiev et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Biosynthesis of polyketide synthase extender units

(2008) Yolande A. Chan et al.   NATURAL PRODUCT REPORTS

Exploiting the mosaic structure of trans-acyltransferase polyketide synthases for natural product discovery and pathway dissection

(2008) TuAnh Nguyen et al.   NATURE BIOTECHNOLOGY

Evolution of polyketide synthases in bacteria

(2008) C. P. Ridley et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Substrate specificity of the acyl transferase domains of EpoC from the epothilone polyketide synthase

(2007) Hrvoje Petković et al.   ORGANIC & BIOMOLECULAR CHEMISTRY