Protein Crystallography in Vaccine Research and Development

Structural and biochemical studies of HCMV gH/gL/gO and Pentamer reveal mutually exclusive cell entry complexes

(2015) Claudio Ciferri et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural Analysis of Bacterial ABC Transporter Inhibition by an Antibody Fragment

(2015) Shivani Ahuja et al.   STRUCTURE

Insights into the trimeric HIV-1 envelope glycoprotein structure

(2015) Andrew B. Ward et al.   TRENDS IN BIOCHEMICAL SCIENCES

Rational stabilization of complex proteins: a divide and combine approach

(2015) Emilio Lamazares et al.   Scientific Reports

Synchrotron radiation macromolecular crystallography: science and spin-offs

(2015) John R. Helliwell et al.   IUCrJ

Solving coiled-coil protein structures

(2015) Zbigniew Dauter   IUCrJ

Serial femtosecond crystallography: the first five years

(2015) Ilme Schlichting   IUCrJ

Crystal and Solution Structure Analysis of FhuD2 from Staphylococcus aureus in Multiple Unliganded Conformations and Bound to Ferrioxamine-B

(2014) Krzysztof J. Podkowa et al.   BIOCHEMISTRY

Apo, Zn2+-bound and Mn2+-bound structures reveal ligand-binding properties of SitA from the pathogenStaphylococcus pseudintermedius

(2014) Francesca Abate et al.   BIOSCIENCE REPORTS

Structural Insights on the Role of Antibodies in HIV-1 Vaccine and Therapy

(2014) Anthony P. West et al.   CELL

Neisseria Adhesin A Variation and Revised Nomenclature Scheme

(2014) Stefania Bambini et al.   Clinical and Vaccine Immunology

Vaccines for the 21st century

(2014) I. Delany et al.   EMBO Molecular Medicine

Current concepts and progress in RSV vaccine development

(2014) Aleks K Guvenel et al.   Expert Review of Vaccines

Respiratory syncytial virus protein structure, function and implications for subunit vaccine development

(2014) Michael McCarthy et al.   Future Virology

Nonbinding Site-Directed Mutants of Transferrin Binding Protein B Exhibit Enhanced Immunogenicity and Protective Capabilities

(2014) Rafael Frandoloso et al.   INFECTION AND IMMUNITY

Proof of principle for epitope-focused vaccine design

(2014) Bruno E. Correia et al.   NATURE

Structure and immune recognition of trimeric pre-fusion HIV-1 Env

(2014) Marie Pancera et al.   NATURE

The promise and challenge of high-throughput sequencing of the antibody repertoire

(2014) George Georgiou et al.   NATURE BIOTECHNOLOGY

Statistical Analysis of Crystallization Database Links Protein Physico-Chemical Features with Crystallization Mechanisms

(2014) Diana Fusco et al.   PLoS One

Structure of the meningococcal vaccine antigen NadA and epitope mapping of a bactericidal antibody

(2014) Enrico Malito et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

History of vaccination

(2014) S. Plotkin   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Developments in X-ray Crystallographic Structure Determination of Biological Macromolecules

(2014) E. F. Garman   SCIENCE

Structure of the large ribosomal subunit from human mitochondria

(2014) A. Brown et al.   SCIENCE

Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein

(2014) Jason Tenboer et al.   SCIENCE

The Discovery and Development of a Novel Vaccine to Protect againstNeisseria meningitidisSerogroup B Disease

(2014) Gary W Zlotnick et al.   Human Vaccines & Immunotherapeutics

Using textons to rank crystallization droplets by the likely presence of crystals

(2014) Jia Tsing Ng et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

A drunken search in crystallization space

(2014) Vincent J. Fazio et al.   Acta Crystallographica Section F-Structural Biology Communications

Optimization of crystallization conditions for biological macromolecules

(2014) Alexander McPherson et al.   Acta Crystallographica Section F-Structural Biology Communications

Covering all the Bases: Preclinical Development of an Effective Staphylococcus aureus Vaccine

(2014) Ingrid L. Scully et al.   Frontiers in Immunology

How the ESRF helps industry and how they help the ESRF

(2013) Stéphanie Malbet-Monaco et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Molecular replacement: tricks and treats

(2013) Chantal Abergel   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Molecular replacement then and now

(2013) Giovanna Scapin   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

New concepts and aids to facilitate crystallization

(2013) Magdalena A Bukowska et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Structural Vaccinology: A Three-dimensional View for Vaccine Development

(2013) Ilaria Ferlenghi et al.   CURRENT TOPICS IN MEDICINAL CHEMISTRY

A Multi-Component Meningococcal Serogroup B Vaccine (4CMenB): The Clinical Development Program

(2013) Miguel O’Ryan et al.   DRUGS

Nonfunctional Variant 3 Factor H Binding Proteins as Meningococcal Vaccine Candidates

(2013) Stijn van der Veen et al.   INFECTION AND IMMUNITY

Three-Dimensional Structure and Biophysical Characterization of Staphylococcus aureus Cell Surface Antigen–Manganese Transporter MntC

(2013) Alexey Gribenko et al.   JOURNAL OF MOLECULAR BIOLOGY

Rapid Screening for Potential Epitopes Reactive with a Polycolonal Antibody by Solution-Phase H/D Exchange Monitored by FT-ICR Mass Spectrometry

(2013) Qian Zhang et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Structure of the TRPV1 ion channel determined by electron cryo-microscopy

(2013) Maofu Liao et al.   NATURE

Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM

(2013) Xueming Li et al.   NATURE METHODS

Isolation of human monoclonal antibodies from peripheral blood B cells

(2013) Jinghe Huang et al.   Nature Protocols

Stabilizing Exposure of Conserved Epitopes by Structure Guided Insertion of Disulfide Bond in HIV-1 Envelope Glycoprotein

(2013) Aemro Kassa et al.   PLoS One

Defining a protective epitope on factor H binding protein, a key meningococcal virulence factor and vaccine antigen

(2013) Enrico Malito et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Architecture of respiratory syncytial virus revealed by electron cryotomography

(2013) L. Liljeroos et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of RSV Fusion Glycoprotein Trimer Bound to a Prefusion-Specific Neutralizing Antibody

(2013) J. S. McLellan et al.   SCIENCE

Serial Femtosecond Crystallography of G Protein-Coupled Receptors

(2013) W. Liu et al.   SCIENCE

Structure-Based Design of a Fusion Glycoprotein Vaccine for Respiratory Syncytial Virus

(2013) J. S. McLellan et al.   SCIENCE

Crystal Structure of a Soluble Cleaved HIV-1 Envelope Trimer

(2013) J.-P. Julien et al.   SCIENCE

Cryo-EM Structure of a Fully Glycosylated Soluble Cleaved HIV-1 Envelope Trimer

(2013) D. Lyumkis et al.   SCIENCE

Rational HIV Immunogen Design to Target Specific Germline B Cell Receptors

(2013) J. Jardine et al.   SCIENCE

Accelerating the development of a safe and effective HIV vaccine: HIV vaccine case study for the Decade of Vaccines

(2013) Wayne C. Koff et al.   VACCINE

A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies

(2013) Rogier W. Sanders et al.   PLoS Pathogens

Collaboration gets the most out of software

(2013) Andrew Morin et al.   eLife

Towards automated crystallographic structure refinement withphenix.refine

(2012) Pavel V. Afonine et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

On the need for an international effort to capture, share and use crystallization screening data

(2012) Janet Newman et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

Structural and functional characterization of theStaphylococcus aureusvirulence factor and vaccine candidate FhuD2

(2012) Paolo Mariotti et al.   BIOCHEMICAL JOURNAL

Staphylococcus aureus Manganese Transport Protein C Is a Highly Conserved Cell Surface Protein That Elicits Protective Immunity Against S. aureus and Staphylococcus epidermidis

(2012) Annaliesa S. Anderson et al.   JOURNAL OF INFECTIOUS DISEASES

Staphylococcus aureus FhuD2 Is Involved in the Early Phase of Staphylococcal Dissemination and Generates Protective Immunity in Mice

(2012) Ravi P. N. Mishra et al.   JOURNAL OF INFECTIOUS DISEASES

Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010

(2012) Rafael Lozano et al.   LANCET

Structural analysis of B-cell epitopes in antibody:protein complexes

(2012) Jens Vindahl Kringelum et al.   MOLECULAR IMMUNOLOGY

The transferrin-iron import system from pathogenicNeisseriaspecies

(2012) Nicholas Noinaj et al.   MOLECULAR MICROBIOLOGY

Structural basis for iron piracy by pathogenic Neisseria

(2012) Nicholas Noinaj et al.   NATURE

Structural vaccinology starts to deliver

(2012) Philip R. Dormitzer et al.   NATURE REVIEWS MICROBIOLOGY

The structural basis of transferrin sequestration by transferrin-binding protein B

(2012) Charles Calmettes et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Immune-Correlates Analysis of an HIV-1 Vaccine Efficacy Trial

(2012) Barton F. Haynes et al.   NEW ENGLAND JOURNAL OF MEDICINE

N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor

(2012) Yaozhong Zou et al.   PLoS One

Neutralizing antibodies against the preactive form of respiratory syncytial virus fusion protein offer unique possibilities for clinical intervention

(2012) M. Magro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography

(2012) S. Boutet et al.   SCIENCE

Fusion Partner Toolchest for the Stabilization and Crystallization of G Protein-Coupled Receptors

(2012) Eugene Chun et al.   STRUCTURE

Fabs Enable Single Particle cryoEM Studies of Small Proteins

(2012) Shenping Wu et al.   STRUCTURE

The Protein Data Bank at 40: Reflecting on the Past to Prepare for the Future

(2012) Helen M. Berman et al.   STRUCTURE

The new multicomponent vaccine against meningococcal serogroup B, 4CMenB: Immunological, functional and structural characterization of the antigens

(2012) Davide Serruto et al.   VACCINE

Meningococcal disease: Clinical presentation and sequelae

(2012) David Pace et al.   VACCINE

The Effect of Human Factor H on Immunogenicity of Meningococcal Native Outer Membrane Vesicle Vaccines with Over-Expressed Factor H Binding Protein

(2012) Peter T. Beernink et al.   PLoS Pathogens

Design and Evaluation of Meningococcal Vaccines through Structure-Based Modification of Host and Pathogen Molecules

(2012) Steven Johnson et al.   PLoS Pathogens

Optimal fine ϕ-slicing for single-photon-counting pixel detectors

(2011) Marcus Mueller et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

A thermal stability assay can help to estimate the crystallization likelihood of biological samples

(2011) Florine Dupeux et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Overview of theCCP4 suite and current developments

(2011) Martyn D. Winn et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Structure of the uncomplexedNeisseria meningitidisfactor H-binding protein fHbp (rLP2086)

(2011) Laura Cendron et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

BIGSdb: Scalable analysis of bacterial genome variation at the population level

(2011) Keith A Jolley et al.   BMC BIOINFORMATICS

Antibody fragments as tools in crystallography

(2011) L. Griffin et al.   CLINICAL AND EXPERIMENTAL IMMUNOLOGY

Complement-Mediated Bactericidal Activity of Anti-Factor H Binding Protein Monoclonal Antibodies against the Meningococcus Relies upon Blocking Factor H Binding

(2011) Serena Giuntini et al.   INFECTION AND IMMUNITY

A Meningococcal Factor H Binding Protein Mutant That Eliminates Factor H Binding Enhances Protective Antibody Responses to Vaccination

(2011) P. T. Beernink et al.   JOURNAL OF IMMUNOLOGY

Design and Characterization of Epitope-Scaffold Immunogens That Present the Motavizumab Epitope from Respiratory Syncytial Virus

(2011) Jason S. McLellan et al.   JOURNAL OF MOLECULAR BIOLOGY

Automated Hydrogen/Deuterium Exchange Electron Transfer Dissociation High Resolution Mass Spectrometry Measured at Single-Amide Resolution

(2011) Rachelle R. Landgraf et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Structure of Respiratory Syncytial Virus Fusion Glycoprotein in the Postfusion Conformation Reveals Preservation of Neutralizing Epitopes

(2011) J. S. McLellan et al.   JOURNAL OF VIROLOGY

Structural basis for immunization with postfusion respiratory syncytial virus fusion F glycoprotein (RSV F) to elicit high neutralizing antibody titers

(2011) K. A. Swanson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Computation-Guided Backbone Grafting of a Discontinuous Motif onto a Protein Scaffold

(2011) M. L. Azoitei et al.   SCIENCE

Rational Design of a Meningococcal Antigen Inducing Broad Protective Immunity

(2011) M. Scarselli et al.   Science Translational Medicine

Application of protein engineering to enhance crystallizability and improve crystal properties

(2010) Zygmunt S. Derewenda   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Impaired Immunogenicity of a Meningococcal Factor H-Binding Protein Vaccine Engineered To Eliminate Factor H Binding

(2010) P. T. Beernink et al.   Clinical and Vaccine Immunology

Correlates of Protection Induced by Vaccination

(2010) S. A. Plotkin   Clinical and Vaccine Immunology

Multicenter, Open‐Label, Randomized Phase II Controlled Trial of an Investigational Recombinant Meningococcal Serogroup B Vaccine With and Without Outer Membrane Vesicles, Administered in Infancy

(2010) Jamie Findlow et al.   CLINICAL INFECTIOUS DISEASES

A structural classification of substrate-binding proteins

(2010) Ronnie P.-A. Berntsson et al.   FEBS LETTERS

Prospects for defined epitope vaccines for respiratory syncytial virus

(2010) Robert Anderson et al.   Future Microbiology

Mapping of the Neisseria meningitidis NadA Cell-Binding Site: Relevance of Predicted  -Helices in the NH2-Terminal and Dimeric Coiled-Coil Regions

(2010) R. Tavano et al.   JOURNAL OF BACTERIOLOGY

Biophysical characterization of recombinant proteins: A key to higher structural genomics success

(2010) Masoud Vedadi et al.   JOURNAL OF STRUCTURAL BIOLOGY

ESPRIT: An automated, library-based method for mapping and soluble expression of protein domains from challenging targets

(2010) Hayretin Yumerefendi et al.   JOURNAL OF STRUCTURAL BIOLOGY

Impact of synchrotron radiation on macromolecular crystallography: a personal view

(2010) Zbigniew Dauter et al.   JOURNAL OF SYNCHROTRON RADIATION

MxCuBE: a synchrotron beamline control environment customized for macromolecular crystallography experiments

(2010) José Gabadinho et al.   JOURNAL OF SYNCHROTRON RADIATION

Structure of a Major Antigenic Site on the Respiratory Syncytial Virus Fusion Glycoprotein in Complex with Neutralizing Antibody 101F

(2010) J. S. McLellan et al.   JOURNAL OF VIROLOGY

Global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systematic review and meta-analysis

(2010) Harish Nair et al.   LANCET

Structural basis of respiratory syncytial virus neutralization by motavizumab

(2010) Jason S McLellan et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Crystal structure of the conserved herpesvirus fusion regulator complex gH–gL

(2010) Tirumala K Chowdary et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Protein structure determination by exhaustive search of Protein Data Bank derived databases

(2010) I. Stokes-Rees et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Elicitation of structure-specific antibodies by epitope scaffolds

(2010) G. Ofek et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Computational Design of Epitope-Scaffolds Allows Induction of Antibodies Specific for a Poorly Immunogenic HIV Vaccine Epitope

(2010) Bruno E. Correia et al.   STRUCTURE

Measuring antigen-specific bactericidal responses to a multicomponent vaccine against serogroup B meningococcus

(2010) Marzia M. Giuliani et al.   VACCINE

Lessons from Structural Genomics

(2009) Thomas C. Terwilliger et al.   Annual Review of Biophysics

Quadrivalent meningococcal ACYW-135 glycoconjugate vaccine for broader protection from infancy

(2009) David Pace   Expert Review of Vaccines

Solution Structure of the Factor H-binding Protein, a Survival Factor and Protective Antigen ofNeisseria meningitidis

(2009) Francesca Cantini et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Neisseria meningitidis recruits factor H using protein mimicry of host carbohydrates

(2009) Muriel C. Schneider et al.   NATURE

Vaccination with ALVAC and AIDSVAX to Prevent HIV-1 Infection in Thailand

(2009) Supachai Rerks-Ngarm et al.   NEW ENGLAND JOURNAL OF MEDICINE

In Situ Proteolysis to Generate Crystals for Structure Determination: An Update

(2009) Amy Wernimont et al.   PLoS One

A coiled-coil motif that sequesters ions to the hydrophobic core

(2009) M. D. Hartmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of Rotavirus Outer-Layer Protein VP7 Bound with a Neutralizing Fab

(2009) S. T. Aoki et al.   SCIENCE

Structure-Based Stabilization of HIV-1 gp120 Enhances Humoral Immune Responses to the Induced Co-Receptor Binding Site

(2009) Barna Dey et al.   PLoS Pathogens

Factor H-Binding Protein Is Important for Meningococcal Survival in Human Whole Blood and Serum and in the Presence of the Antimicrobial Peptide LL-37

(2008) K. L. Seib et al.   INFECTION AND IMMUNITY

Structural Basis for the Immunogenic Properties of the Meningococcal Vaccine Candidate LP2086

(2008) Alessandro Mascioni et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Protein crystallization: from purified protein to diffraction-quality crystal

(2008) Naomi E Chayen et al.   NATURE METHODS

Large-scale evaluation of protein reductive methylation for improving protein crystallization

(2008) Youngchang Kim et al.   NATURE METHODS

Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction

(2008) X. Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Toward chaperone-assisted crystallography: Protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold

(2008) Valentina Tereshko et al.   PROTEIN SCIENCE