Extending the biosynthetic repertoires of cyanobacteria and chloroplasts

Engineering the methylerythritol phosphate pathway in cyanobacteria for photosynthetic isoprene production from CO2

(2016) Xiang Gao et al.   Energy & Environmental Science

Transfer of the cytochrome P450-dependent dhurrin pathway fromSorghum bicolorintoNicotiana tabacumchloroplasts for light-driven synthesis

(2016) Thiyagarajan Gnanasekaran et al.   JOURNAL OF EXPERIMENTAL BOTANY

Biosynthesis of platform chemical 3-hydroxypropionic acid (3-HP) directly from CO 2 in cyanobacterium Synechocystis sp. PCC 6803

(2016) Yunpeng Wang et al.   METABOLIC ENGINEERING

Metabolic engineering of light-driven cytochrome P450 dependent pathways into Synechocystis sp. PCC 6803

(2016) Artur Wlodarczyk et al.   METABOLIC ENGINEERING

Fine-Tuning of Photoautotrophic Protein Production by Combining Promoters and Neutral Sites in the Cyanobacterium Synechocystis sp. Strain PCC 6803

(2015) Andrew H. Ng et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Insights into chloroplast biogenesis and development

(2015) Barry J. Pogson et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Engineering a novel biosynthetic pathway in Escherichia coli for production of renewable ethylene glycol

(2015) Brian Pereira et al.   BIOTECHNOLOGY AND BIOENGINEERING

Engineering cyanobacteria for direct biofuel production from CO 2

(2015) Philipp Savakis et al.   CURRENT OPINION IN BIOTECHNOLOGY

Improving aSynechocystis-based photoautotrophic chassis through systematic genome mapping and validation of neutral sites

(2015) Filipe Pinto et al.   DNA RESEARCH

The Development and Characterization of an Exogenous Green-Light-Regulated Gene Expression System in Marine Cyanobacteria

(2015) Amr Badary et al.   MARINE BIOTECHNOLOGY

Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via β-alanine

(2015) Irina Borodina et al.   METABOLIC ENGINEERING

Establishment of a yeast platform strain for production of p- coumaric acid through metabolic engineering of aromatic amino acid biosynthesis

(2015) Angelica Rodriguez et al.   METABOLIC ENGINEERING

Metabolic engineering of cyanobacteria for photosynthetic 3-hydroxypropionic acid production from CO 2 using Synechococcus elongatus PCC 7942

(2015) Ethan I. Lan et al.   METABOLIC ENGINEERING

A phycocyanin·phellandrene synthase fusion enhances recombinant protein expression and β-phellandrene (monoterpene) hydrocarbons production in Synechocystis (cyanobacteria)

(2015) Cinzia Formighieri et al.   METABOLIC ENGINEERING

Biological targets and mechanisms of action of natural products from marine cyanobacteria

(2015) Lilibeth A. Salvador-Reyes et al.   NATURAL PRODUCT REPORTS

Expression and membrane-targeting of an active plant cytochrome P450 in the chloroplast of the green alga Chlamydomonas reinhardtii

(2015) Doris Gangl et al.   PHYTOCHEMISTRY

Moss-made pharmaceuticals: from bench to bedside

(2015) Ralf Reski et al.   PLANT BIOTECHNOLOGY JOURNAL

Sclareol production in the moss Physcomitrella patens and observations on growth and terpenoid biosynthesis

(2015) Xi-Wu Pan et al.   Plant Biotechnology Reports

Engineering Triterpene and Methylated Triterpene Production in Plants Provides Biochemical and Physiological Insights into Terpene Metabolism

(2015) Zuodong Jiang et al.   PLANT PHYSIOLOGY

Additional diterpenes from Physcomitrella patens synthesized by copalyl diphosphate/kaurene synthase ( Pp CPS/KS)

(2015) Xin Zhan et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone

(2015) Warren Lau et al.   SCIENCE

Natural Product Biosynthetic Diversity and Comparative Genomics of the Cyanobacteria

(2015) Elke Dittmann et al.   TRENDS IN MICROBIOLOGY

Flavonoid transport mechanisms: how to go, and with whom

(2015) Jian Zhao   TRENDS IN PLANT SCIENCE

Multiple Gene Repression in Cyanobacteria Using CRISPRi

(2015) Lun Yao et al.   ACS Synthetic Biology

Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide

(2015) Elias Englund et al.   ACS Synthetic Biology

Stable heterologous expression of biologically active terpenoids in green plant cells

(2015) N. Kusaira B. K. Ikram et al.   Frontiers in Plant Science

Heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana and the effect of N-terminal modifications of the involved cytochrome P450 enzyme

(2015) Thiyagarajan Gnanasekaran et al.   Journal of Biological Engineering

Scaffold-fused riboregulators for enhanced gene activation inSynechocystissp. PCC 6803

(2015) Yuta Sakai et al.   MicrobiologyOpen

The plasticity of cyanobacterial metabolism supports direct CO2 conversion to ethylene

(2015) Wei Xiong et al.   Nature Plants

Regulation of Gene Expression in Diverse Cyanobacterial Species by Using Theophylline-Responsive Riboswitches

(2014) Amy T. Ma et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Improved production of fatty alcohols in cyanobacteria by metabolic engineering

(2014) Lun Yao et al.   Biotechnology for Biofuels

Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803

(2014) S Angermayr et al.   Biotechnology for Biofuels

A green-light inducible lytic system for cyanobacterial cells

(2014) Kotone Miyake et al.   Biotechnology for Biofuels

Metabolic engineering of chloroplasts for artemisinic acid biosynthesis and impact on plant growth

(2014) Bhawna Saxena et al.   JOURNAL OF BIOSCIENCES

Bridging the gap: Membrane contact sites in signaling, metabolism, and organelle dynamics

(2014) William A. Prinz   JOURNAL OF CELL BIOLOGY

Phenol homeostasis is ensured in vanilla fruit by storage under solid form in a new chloroplast-derived organelle, the phenyloplast

(2014) Jean-Marc Brillouet et al.   JOURNAL OF EXPERIMENTAL BOTANY

Elucidation and in planta reconstitution of the parthenolide biosynthetic pathway

(2014) Qing Liu et al.   METABOLIC ENGINEERING

Biosynthesis of β-carotene in engineered E. coli using the MEP and MVA pathways

(2014) Jianming Yang et al.   Microbial Cell Factories

Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development

(2014) Chris J. Paddon et al.   NATURE REVIEWS MICROBIOLOGY

Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria

(2014) Arnaud Taton et al.   NUCLEIC ACIDS RESEARCH

Production of Squalene in Synechocystis sp. PCC 6803

(2014) Elias Englund et al.   PLoS One

Anchoring a Plant Cytochrome P450 via PsaM to the Thylakoids in Synechococcus sp. PCC 7002: Evidence for Light-Driven Biosynthesis

(2014) Lærke Münter Lassen et al.   PLoS One

Redefining the metabolic continuity of chloroplasts and ER

(2014) Payam Mehrshahi et al.   TRENDS IN PLANT SCIENCE

Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria

(2014) Jie Zhou et al.   Scientific Reports

Design and analysis of LacI-repressed promoters and DNA-looping in a cyanobacterium

(2014) Daniel Camsund et al.   Journal of Biological Engineering

The tannosome is an organelle forming condensed tannins in the chlorophyllous organs of Tracheophyta

(2013) Jean-Marc Brillouet et al.   ANNALS OF BOTANY

Network Analysis of the MVA and MEP Pathways for Isoprenoid Synthesis

(2013) Eva Vranová et al.   Annual Review of Plant Biology

Engineering cyanobacteria to improve photosynthetic production of alka(e)nes

(2013) Weihua Wang et al.   Biotechnology for Biofuels

Computational evaluation ofSynechococcussp. PCC 7002 metabolism for chemical production

(2013) Trang T. Vu et al.   Biotechnology Journal

Genetic engineering of the chloroplast: novel tools and new applications

(2013) Ralph Bock   CURRENT OPINION IN BIOTECHNOLOGY

Carbon partitioning in photosynthesis

(2013) Anastasios Melis   CURRENT OPINION IN CHEMICAL BIOLOGY

Geraniol hydroxylase and hydroxygeraniol oxidase activities of the CYP76 family of cytochrome P450 enzymes and potential for engineering the early steps of the (seco)iridoid pathway

(2013) René Höfer et al.   METABOLIC ENGINEERING

Engineering cyanobacteria for photosynthetic production of 3-hydroxybutyrate directly from CO2

(2013) Bo Wang et al.   METABOLIC ENGINEERING

Engineering of photosynthetic mannitol biosynthesis from CO2 in a cyanobacterium

(2013) Jacob H. Jacobsen et al.   METABOLIC ENGINEERING

Engineering a cyanobacterium as the catalyst for the photosynthetic conversion of CO2 to 1,2-propanediol

(2013) Han Li et al.   Microbial Cell Factories

Heterologous Expression of the Mevalonic Acid Pathway in Cyanobacteria Enhances Endogenous Carbon Partitioning to Isoprene

(2013) Fiona K. Bentley et al.   Molecular Plant

Plant P450s as versatile drivers for evolution of species-specific chemical diversity

(2013) B. Hamberger et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Theophylline-Dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC 7942

(2013) Yoichi Nakahira et al.   PLANT AND CELL PHYSIOLOGY

Efficient metabolic pathway engineering in transgenic tobacco and tomato plastids with synthetic multigene operons

(2013) Y. Lu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Transorganellar complementation redefines the biochemical continuity of endoplasmic reticulum and chloroplasts

(2013) P. Mehrshahi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cyanobacterial conversion of carbon dioxide to 2,3-butanediol

(2013) John W. K. Oliver et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An alternative pathway contributes to phenylalanine biosynthesis in plants via a cytosolic tyrosine:phenylpyruvate aminotransferase

(2013) Heejin Yoo et al.   Nature Communications

Improving accuracy of cell and chromophore concentration measurements using optical density

(2013) John A Myers et al.   BMC Biophysics

Homologous recombination-mediated gene targeting in the liverwort Marchantia polymorpha L.

(2013) Kimitsune Ishizaki et al.   Scientific Reports

Redirecting Photosynthetic Electron Flow into Light-Driven Synthesis of Alternative Products Including High-Value Bioactive Natural Compounds

(2013) Lærke Münter Lassen et al.   ACS Synthetic Biology

Redirecting Photosynthetic Reducing Power toward Bioactive Natural Product Synthesis

(2013) Agnieszka Zygadlo Nielsen et al.   ACS Synthetic Biology

Flux Balance Analysis of Cyanobacterial Metabolism: The Metabolic Network of Synechocystis sp. PCC 6803

(2013) Henning Knoop et al.   PLoS Computational Biology

Synthetic biology of cyanobacteria: unique challenges and opportunities

(2013) Bertram M. Berla et al.   Frontiers in Microbiology

Shikimate and Phenylalanine Biosynthesis in the Green Lineage

(2013) Takayuki Tohge et al.   Frontiers in Plant Science

The Shikimate Pathway and Aromatic Amino Acid Biosynthesis in Plants

(2012) Hiroshi Maeda et al.   Annual Review of Plant Biology

Metabolic Engineering of Synechocystis sp. Strain PCC 6803 for Isobutanol Production

(2012) Arul M. Varman et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Rerouting Carbon Flux To Enhance Photosynthetic Productivity

(2012) Daniel C. Ducat et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Isoprene Production Via the Mevalonic Acid Pathway in Escherichia coli (Bacteria)

(2012) Andreas Zurbriggen et al.   BioEnergy Research

A Gene-Fusion Approach to Enabling Plant Cytochromes P450 for Biocatalysis

(2012) Julia Schückel et al.   CHEMBIOCHEM

Photosynthetic production of 2-methyl-1-butanol from CO2 in cyanobacterium Synechococcus elongatus PCC7942 and characterization of the native acetohydroxyacid synthase

(2012) Claire R. Shen et al.   Energy & Environmental Science

Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria

(2012) Zhengxu Gao et al.   Energy & Environmental Science

Bioengineering of carbon fixation, biofuels, and biochemicals in cyanobacteria and plants

(2012) Lisa Rosgaard et al.   JOURNAL OF BIOTECHNOLOGY

Chloroplast transformation for engineering of photosynthesis

(2012) Maureen R. Hanson et al.   JOURNAL OF EXPERIMENTAL BOTANY

Characterization of Stress Responses of Heavy Metal and Metalloid Inducible Promoters in Synechocystis PCC6803

(2012) Barbara Blasi   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Designing and using RNA scaffolds to assemble proteins in vivo

(2012) Camille J Delebecque et al.   Nature Protocols

Plant type ferredoxins and ferredoxin-dependent metabolism

(2012) GUY HANKE et al.   PLANT CELL AND ENVIRONMENT

Exceptional inheritance of plastids via pollen inNicotiana sylvestriswith no detectable paternal mitochondrial DNA in the progeny

(2012) Gregory Thyssen et al.   PLANT JOURNAL

Plastid Proteostasis and Heterologous Protein Accumulation in Transplastomic Plants

(2012) F. De Marchis et al.   PLANT PHYSIOLOGY

Detailing the optimality of photosynthesis in cyanobacteria through systems biology analysis

(2012) J. Nogales et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Synthetic Oligonucleotide Libraries Reveal Novel Regulatory Elements in Chlamydomonas Chloroplast mRNAs

(2012) Elizabeth A. Specht et al.   ACS Synthetic Biology

Synthetic Biology and Metabolic Engineering

(2012) Gregory Stephanopoulos   ACS Synthetic Biology

Genome-Scale Modeling of Light-Driven Reductant Partitioning and Carbon Fluxes in Diazotrophic Unicellular Cyanobacterium Cyanothece sp. ATCC 51142

(2012) Trang T. Vu et al.   PLoS Computational Biology

Light-Driven Cytochrome P450 Hydroxylations

(2011) Kenneth Jensen et al.   ACS Chemical Biology

Convergent Evolution in Plant Specialized Metabolism

(2011) Eran Pichersky et al.   Annual Review of Plant Biology

Diffusion-based process for carbon dioxide uptake and isoprene emission in gaseous/aqueous two-phase photobioreactors by photosynthetic microorganisms

(2011) Fiona K. Bentley et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cytochrome P450: taming a wild type enzyme

(2011) Sang Taek Jung et al.   CURRENT OPINION IN BIOTECHNOLOGY

Synthetic scaffolds increased resveratrol biosynthesis in engineered yeast cells

(2011) Yechun Wang et al.   JOURNAL OF BIOTECHNOLOGY

Spatial organization of enzymes for metabolic engineering

(2011) Hanson Lee et al.   METABOLIC ENGINEERING

The chloroplast transformation toolbox: selectable markers and marker removal

(2011) Anil Day et al.   PLANT BIOTECHNOLOGY JOURNAL

Optimization of the expression of the HIV fusion inhibitor cyanovirin-N from the tobacco plastid genome

(2011) Zouhair Elghabi et al.   PLANT BIOTECHNOLOGY JOURNAL

Plastid Biotechnology: Food, Fuel, and Medicine for the 21st Century

(2011) P. Maliga et al.   PLANT PHYSIOLOGY

Photosynthetic electron partitioning between [FeFe]-hydrogenase and ferredoxin:NADP+-oxidoreductase (FNR) enzymes in vitro

(2011) I. Yacoby et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An experimentally anchored map of transcriptional start sites in the model cyanobacteriumSynechocystissp. PCC6803

(2011) Jan Mitschke et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Organization of Intracellular Reactions with Rationally Designed RNA Assemblies

(2011) C. J. Delebecque et al.   SCIENCE

Cytochrome P450 monooxygenases: an update on perspectives for synthetic application

(2011) Vlada B. Urlacher et al.   TRENDS IN BIOTECHNOLOGY

Local Absence of Secondary Structure Permits Translation of mRNAs that Lack Ribosome-Binding Sites

(2011) Lars B. Scharff et al.   PLoS Genetics

FdC1, a Novel Ferredoxin Protein Capable of Alternative Electron Partitioning, Increases in Conditions of Acceptor Limitation at Photosystem I

(2010) Ingo Voss et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The production of the sesquiterpene β-caryophyllene in a transgenic strain of the cyanobacterium Synechocystis

(2010) Robert E. Reinsvold et al.   JOURNAL OF PLANT PHYSIOLOGY

Use of modular, synthetic scaffolds for improved production of glucaric acid in engineered E. coli

(2010) Tae Seok Moon et al.   METABOLIC ENGINEERING

New Insights into the Shikimate and Aromatic Amino Acids Biosynthesis Pathways in Plants

(2010) Vered Tzin et al.   Molecular Plant

What is flux balance analysis?

(2010) Jeffrey D Orth et al.   NATURE BIOTECHNOLOGY

Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology

(2010) Hsin-Ho Huang et al.   NUCLEIC ACIDS RESEARCH

Improved foreign gene expression in plants using a virus-encoded suppressor of RNA silencing modified to be developmentally harmless

(2010) Pooja Saxena et al.   PLANT BIOTECHNOLOGY JOURNAL

Rapid expression of transgenes driven by seed-specific constructs in leaf tissue: DHA production

(2010) James R Petrie et al.   Plant Methods

Chloramphenicol acetyltransferase as selectable marker for plastid transformation

(2010) Weimin Li et al.   PLANT MOLECULAR BIOLOGY

Endogenous Diterpenes Derived from ent-Kaurene, a Common Gibberellin Precursor, Regulate Protonema Differentiation of the Moss Physcomitrella patens

(2010) K.-i. Hayashi et al.   PLANT PHYSIOLOGY

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368

(2009) Guillermina Goñi et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde

(2009) Shota Atsumi et al.   NATURE BIOTECHNOLOGY

Synthetic protein scaffolds provide modular control over metabolic flux

(2009) John E Dueber et al.   NATURE BIOTECHNOLOGY

A leaf-based assay using interchangeable design principles to rapidly assemble multistep recombinant pathways

(2009) Craig C. Wood et al.   PLANT BIOTECHNOLOGY JOURNAL

Solar energy conversion efficiencies in photosynthesis: Minimizing the chlorophyll antennae to maximize efficiency

(2009) Anastasios Melis   PLANT SCIENCE

Engineering the spatial organization of metabolic enzymes: mimicking nature's synergy

(2008) Robert J Conrado et al.   CURRENT OPINION IN BIOTECHNOLOGY

Molecular Design of Photosynthesis-Elevated Chloroplasts for Mass Accumulation of a Foreign Protein

(2008) Y. Yabuta et al.   PLANT AND CELL PHYSIOLOGY

Exhaustion of the chloroplast protein synthesis capacity by massive expression of a highly stable protein antibiotic

(2008) Melanie Oey et al.   PLANT JOURNAL