Application of Human Brain Organoids—Opportunities and Challenges in Modeling Human Brain Development and Neurodevelopmental Diseases

Engineering Human Brain Assembloids by Microfluidics

(2023) Yujuan Zhu et al.   ADVANCED MATERIALS

Recent Advances in Generation of In Vitro Cardiac Organoids

(2023) Makoto Sahara   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Midbrain organoids mimic early embryonic neurodevelopment and recapitulate LRRK2-p.Gly2019Ser-associated gene expression

(2022) Alise Zagare et al.   AMERICAN JOURNAL OF HUMAN GENETICS

Challenges of Organoid Research

(2022) Madeline G. Andrews et al.   Annual Review of Neuroscience

Autism genes converge on asynchronous development of shared neuron classes

(2022) Bruna Paulsen et al.   NATURE

Expression of the transcription factor PU.1 induces the generation of microglia-like cells in human cortical organoids

(2022) Bilal Cakir et al.   Nature Communications

Cortical–Hypothalamic Integration of Autonomic and Endocrine Stress Responses

(2022) Derek Schaeuble et al.   Frontiers in Physiology

Generation of vascularized brain organoids to study neurovascular interactions

(2022) Xin-Yao Sun et al.   eLife

Tropism of SARS-CoV-2 for human cortical astrocytes

(2022) Madeline G. Andrews et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The presence of BBB hastens neuronal differentiation of cerebral organoids – The potential role of endothelial derived BDNF

(2022) Giorgia Fedele et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Maturation and circuit integration of transplanted human cortical organoids

(2022) Omer Revah et al.   NATURE

Microglia-containing human brain organoids for the study of brain development and pathology

(2022) Wendiao Zhang et al.   MOLECULAR PSYCHIATRY

Advancing Drug Discovery for Neurological Disorders Using iPSC-Derived Neural Organoids

(2021) Gianluca Costamagna et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Long-term maturation of human cortical organoids matches key early postnatal transitions

(2021) Aaron Gordon et al.   NATURE NEUROSCIENCE

Engineering organoids

(2021) Moritz Hofer et al.   Nature Reviews Materials

Neurodevelopmental defects and neurodegenerative phenotypes in human brain organoids carrying Parkinson’s disease-linked DNAJC6 mutations

(2021) Noviana Wulansari et al.   Science Advances

An early cell shape transition drives evolutionary expansion of the human forebrain

(2021) Silvia Benito-Kwiecinski et al.   CELL

Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia

(2021) Ugomma C. Eze et al.   NATURE NEUROSCIENCE

Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids

(2021) Galina Popova et al.   Cell Stem Cell

3D biomaterial models of human brain disease

(2021) Janko Kajtez et al.   NEUROCHEMISTRY INTERNATIONAL

Neural stem cells derived from human midbrain organoids as a stable source for treating Parkinson’s disease

(2021) Seung Won Kim et al.   PROGRESS IN NEUROBIOLOGY

Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids

(2021) Ann-Na Cho et al.   Nature Communications

Hepatocyte organoids and cell transplantation: What the future holds

(2021) Weng Chuan Peng et al.   EXPERIMENTAL AND MOLECULAR MEDICINE

Schizophrenia is defined by cell-specific neuropathology and multiple neurodevelopmental mechanisms in patient-derived cerebral organoids

(2021) Michael Notaras et al.   MOLECULAR PSYCHIATRY

Single-cell transcriptomics captures features of human midbrain development and dopamine neuron diversity in brain organoids

(2021) Alessandro Fiorenzano et al.   Nature Communications

Reducing Hypothalamic Stem Cell Senescence Protects against Aging-Associated Physiological Decline

(2020) Yu-Zhong Xiao et al.   Cell Metabolism

Cell stress in cortical organoids impairs molecular subtype specification

(2020) Aparna Bhaduri et al.   NATURE

Potassium channel dysfunction in human neuronal models of Angelman syndrome

(2020) Alfred Xuyang Sun et al.   SCIENCE

Hypothalamic Contribution to Pituitary Functions Is Recapitulated In Vitro Using 3D-Cultured Human iPS Cells

(2020) Takatoshi Kasai et al.   Cell Reports

Sliced Human Cortical Organoids for Modeling Distinct Cortical Layer Formation

(2020) Xuyu Qian et al.   Cell Stem Cell

Vascularized human cortical organoids (vOrganoids) model cortical development in vivo

(2020) Yingchao Shi et al.   PLOS BIOLOGY

Midbrain Organoids: A New Tool to Investigate Parkinson’s Disease

(2020) Lisa Maria Smits et al.   Frontiers in Cell and Developmental Biology

Loss of function of the mitochondrial peptidase PITRM1 induces proteotoxic stress and Alzheimer’s disease-like pathology in human cerebral organoids

(2020) María José Pérez et al.   MOLECULAR PSYCHIATRY

Human organoids: model systems for human biology and medicine

(2020) Jihoon Kim et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Human CNS barrier-forming organoids with cerebrospinal fluid production

(2020) Laura Pellegrini et al.   SCIENCE

Asynchronous mixing of kidney progenitor cells potentiates nephrogenesis in organoids

(2020) Ashwani Kumar Gupta et al.   Communications Biology

Dissecting Alzheimer’s disease pathogenesis in human 2D and 3D models

(2020) Giovanna Cenini et al.   MOLECULAR AND CELLULAR NEUROSCIENCE

Generation of Functional Human 3D Cortico-Motor Assembloids

(2020) Jimena Andersen et al.   CELL

Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells

(2020) Yuki Miura et al.   NATURE BIOTECHNOLOGY

Modeling G2019S-LRRK2 Sporadic Parkinson's Disease in 3D Midbrain Organoids

(2019) Hongwon Kim et al.   Stem Cell Reports

Induced pluripotent stem cells in disease modelling and drug discovery

(2019) R. Grant Rowe et al.   NATURE REVIEWS GENETICS

Cerebral organoids at the air–liquid interface generate diverse nerve tracts with functional output

(2019) Stefano L. Giandomenico et al.   NATURE NEUROSCIENCE

Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells

(2019) Elena Garreta et al.   NATURE MATERIALS

hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids

(2019) Yangfei Xiang et al.   Cell Stem Cell

Establishing Cerebral Organoids as Models of Human-Specific Brain Evolution

(2019) Alex A. Pollen et al.   CELL

Human 3D cellular model of hypoxic brain injury of prematurity

(2019) Anca M. Pașca et al.   NATURE MEDICINE

Individual brain organoids reproducibly form cell diversity of the human cerebral cortex

(2019) Silvia Velasco et al.   NATURE

Brain Organoids as Tools for Modeling Human Neurodevelopmental Disorders

(2019) Jason W. Adams et al.   PHYSIOLOGY

LIN 28A loss of function is associated with Parkinson's disease pathogenesis

(2019) Mi‐Yoon Chang et al.   EMBO JOURNAL

Organoid single-cell genomic atlas uncovers human-specific features of brain development

(2019) Sabina Kanton et al.   NATURE

Engineering of human brain organoids with a functional vascular-like system

(2019) Bilal Cakir et al.   NATURE METHODS

Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities

(2019) Juliana Minardi Nascimento et al.   Frontiers in Cell and Developmental Biology

Organoid and Assembloid Technologies for Investigating Cellular Crosstalk in Human Brain Development and Disease

(2019) Rebecca M. Marton et al.   TRENDS IN CELL BIOLOGY

Neural stem cell therapy - brief review

(2018) Cezary Grochowski et al.   CLINICAL NEUROLOGY AND NEUROSURGERY

Brain-derived neurotrophic factor secreted by the cerebral endothelium: A new actor of brain function?

(2018) Christine Marie et al.   JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM

Molecular windows into the human brain for psychiatric disorders

(2018) Gabor Egervari et al.   MOLECULAR PSYCHIATRY

Human brain organoids on a chip reveal the physics of folding

(2018) Eyal Karzbrun et al.   Nature Physics

APOE4 Causes Widespread Molecular and Cellular Alterations Associated with Alzheimer’s Disease Phenotypes in Human iPSC-Derived Brain Cell Types

(2018) Yuan-Ta Lin et al.   NEURON

Activity of translation regulator eukaryotic elongation factor-2 kinase is increased in Parkinson disease brain and its inhibition reduces alpha synuclein toxicity

(2018) Asad Jan et al.   Acta Neuropathologica Communications

Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons

(2018) Halil Tekin et al.   Nature Biomedical Engineering

Modeling amyloid beta and tau pathology in human cerebral organoids

(2018) Cesar Gonzalez et al.   MOLECULAR PSYCHIATRY

Animal models of neurodegenerative diseases

(2018) Ted M. Dawson et al.   NATURE NEUROSCIENCE

Generation and assembly of human brain region–specific three-dimensional cultures

(2018) Steven A. Sloan et al.   Nature Protocols

Human Models Are Needed for Studying Human Neurodevelopmental Disorders

(2018) Xinyu Zhao et al.   AMERICAN JOURNAL OF HUMAN GENETICS

Long-Term Expansion of Functional Mouse and Human Hepatocytes as 3D Organoids

(2018) Huili Hu et al.   CELL

Depression

(2018) Gin S Malhi et al.   LANCET

Transcriptome and epigenome landscape of human cortical development modeled in organoids

(2018) Anahita Amiri et al.   SCIENCE

MeCP2-regulated miRNAs control early human neurogenesis through differential effects on ERK and AKT signaling

(2017) N Mellios et al.   MOLECULAR PSYCHIATRY

Hypothalamic stem cells control ageing speed partly through exosomal miRNAs

(2017) Yalin Zhang et al.   NATURE

Assembly of functionally integrated human forebrain spheroids

(2017) Fikri Birey et al.   NATURE

Fused cerebral organoids model interactions between brain regions

(2017) Joshua A Bagley et al.   NATURE METHODS

iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases

(2017) Edsel M. Abud et al.   NEURON

Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem Cells

(2017) Steven A. Sloan et al.   NEURON

Derivation of Human Midbrain-Specific Organoids from Neuroepithelial Stem Cells

(2017) Anna S. Monzel et al.   Stem Cell Reports

Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure

(2016) Xuyu Qian et al.   CELL

Midbrain-like Organoids from Human Pluripotent Stem Cells Contain Functional Dopaminergic and Neuromelanin-Producing Neurons

(2016) Junghyun Jo et al.   Cell Stem Cell

New insights on the role of microglia in synaptic pruning in health and disease

(2016) Soyon Hong et al.   CURRENT OPINION IN NEUROBIOLOGY

CPAP promotes timely cilium disassembly to maintain neural progenitor pool

(2016) Elke Gabriel et al.   EMBO JOURNAL

Neurodegenerative diseases

(2016) Marie-Thérèse Heemels   NATURE

Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells

(2016) Chikafumi Ozone et al.   Nature Communications

Disruption of an Evolutionarily Novel Synaptic Expression Pattern in Autism

(2016) Xiling Liu et al.   PLOS BIOLOGY

Self-Organizing 3D Human Neural Tissue Derived from Induced Pluripotent Stem Cells Recapitulate Alzheimer’s Disease Phenotypes

(2016) Waseem K. Raja et al.   PLoS One

The Flaws and Human Harms of Animal Experimentation

(2015) AYSHA AKHTAR   CAMBRIDGE QUARTERLY OF HEALTHCARE ETHICS

Human cerebral organoids recapitulate gene expression programs of fetal neocortex development

(2015) J. Gray Camp et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue

(2015) Hideya Sakaguchi et al.   Nature Communications

Self-Organization of Polarized Cerebellar Tissue in 3D Culture of Human Pluripotent Stem Cells

(2015) Keiko Muguruma et al.   Cell Reports

Generation of cerebral organoids from human pluripotent stem cells

(2014) Madeline A Lancaster et al.   Nature Protocols

Transcriptome meta-analysis reveals a central role for sex steroids in the degeneration of hippocampal neurons in Alzheimer’s disease

(2013) Jessica M Winkler et al.   BMC Systems Biology

Loss of dopaminergic nigrostriatal neurons accounts for the motivational and affective deficits in Parkinson’s disease

(2013) G Drui et al.   MOLECULAR PSYCHIATRY

Cerebral organoids model human brain development and microcephaly

(2013) Madeline A. Lancaster et al.   NATURE

Corticostriatal connectivity and its role in disease

(2013) Gordon M. G. Shepherd   NATURE REVIEWS NEUROSCIENCE

Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex

(2013) T. Kadoshima et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Tanycytes of the hypothalamic median eminence form a diet-responsive neurogenic niche

(2012) Daniel A Lee et al.   NATURE NEUROSCIENCE

Modeling human cortical development in vitro using induced pluripotent stem cells

(2012) J. Mariani et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Self-organizing optic-cup morphogenesis in three-dimensional culture

(2011) Mototsugu Eiraku et al.   NATURE

Endocytosis and clathrin-uncoating defects at synapses of auxilin knockout mice

(2010) Y.-I. Yim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals

(2008) Mototsugu Eiraku et al.   Cell Stem Cell