Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 626, Issue -, Pages 30-37Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2022.07.112
Keywords
Induced pluripotent stem cells; Cerebral organoids; Blood-brain barrier; BDNF
Categories
Funding
- Universita? di Milano (Fondi del Piano di Sviluppo di Ricerca 2021)
- Regione Lombardia under the program Call Hub Ricerca e Innovazione [1170989-PRINTMED-3D]
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Despite being the best in vitro model for the human brain, human cerebral organoids lack the endothelial component of the blood-brain barrier (BBB). This study proposes a co-culture of BBB and cerebral organoids to improve their organization. The presence of BBB enhances the maturation of cerebral organoids, possibly through the release of the neurotrophic factor BDNF.
Despite remaining the best in vitro model to resemble the human brain, a weakness of human cerebral organoids is the lack of the endothelial component that in vivo organizes in the blood brain barrier (BBB). Since the BBB is crucial to control the microenvironment of the nervous system, this study proposes a co -culture of BBB and cerebral organoids. We utilized a BBB model consisting of primary human brain microvascular endothelial cells and astrocytes in a transwell system. Starting from induced Pluripotent Stem Cells (iPSCs) we generated human cerebral organoids which were then cultured in the absence or presence of an in vitro model of BBB to evaluate potential effects on the maturation of cerebral organoids. By morphological analysis, it emerges that in the presence of the BBB the cerebral organoids are better organized than controls in the absence of the BBB. This effect might be due to Brain Derived Neurotrophic Factor (BDNF), a neurotrophic factor released by the endothelial component of the BBB, which is involved in neurodevelopment, neuroplasticity and neurosurvival.(c) 2022 Elsevier Inc. All rights reserved.
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