The ever-growing world of gastruloids: autogenous models of mammalian embryogenesis

Controlling human organoid symmetry breaking reveals signaling gradients drive segmentation clock waves

(2023) Yusuf Ilker Yaman et al.   CELL

Controlling organoid symmetry breaking uncovers an excitable system underlying human axial elongation

(2023) Giridhar M. Anand et al.   CELL

Multimodal characterization of murine gastruloid development

(2023) Simon Suppinger et al.   Cell Stem Cell

A stem cell zoo uncovers intracellular scaling of developmental tempo across mammals

(2023) Jorge Lázaro et al.   Cell Stem Cell

Toward better assessments of developmental toxicity using stem cell‐based in vitro embryogenesis models

(2022) Yusuke Marikawa   Birth Defects Research

Periodic formation of epithelial somites from human pluripotent stem cells

(2022) Marina Sanaki-Matsumiya et al.   Nature Communications

Cell-state transitions and collective cell movement generate an endoderm-like region in gastruloids

(2022) Ali Hashmi et al.   eLife

Gastruloids as in vitro models of embryonic blood development with spatial and temporal resolution

(2022) Giuliana Rossi et al.   Scientific Reports

Chimeric 3D-gastruloids – a versatile tool for studies of mammalian peri-gastrulation development

(2022) Alexandra E. Wehmeyer et al.   DEVELOPMENT

Hypoxia induces an early primitive streak signature, enhancing spontaneous elongation and lineage representation in gastruloids

(2022) Natalia López-Anguita et al.   DEVELOPMENT

Stabilization of cell-cell adhesions prevents symmetry breaking and locks in pluripotency in 3D gastruloids

(2022) Federica Cermola et al.   Stem Cell Reports

Reconstruction and deconstruction of human somitogenesis in vitro

(2022) Yuchuan Miao et al.   NATURE

Reconstituting human somitogenesis in vitro

(2022) Yoshihiro Yamanaka et al.   NATURE

Opportunities and Challenges with Stem Cell-Based Embryo Models

(2021) Janet Rossant et al.   Stem Cell Reports

Gastruloids generated without exogenous Wnt activation develop anterior neural tissues

(2021) Mehmet U. Girgin et al.   Stem Cell Reports

Axial elongation of caudalized human organoids mimics aspects of neural tube development

(2021) Ashley R. G. Libby et al.   DEVELOPMENT

Depletion of Aneuploid Cells in Human Embryos and Gastruloids

(2021) Min Yang et al.   OBSTETRICAL & GYNECOLOGICAL SURVEY

3D gastruloids: a novel frontier in stem cell-based in vitro modeling of mammalian gastrulation

(2021) Susanne C. van den Brink et al.   TRENDS IN CELL BIOLOGY

Co-development of central and peripheral neurons with trunk mesendoderm in human elongating multi-lineage organized gastruloids

(2021) Zachary T. Olmsted et al.   Nature Communications

BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo

(2021) Sapna Chhabra et al.   Biology Open

Human neural tube morphogenesis in vitro by geometric constraints

(2021) Eyal Karzbrun et al.   NATURE

In vitro teratogenicity testing using a 3D, embryo-like gastruloid system

(2021) Veronika Mantziou et al.   REPRODUCTIVE TOXICOLOGY

Single-cell and spatial transcriptomics reveal somitogenesis in gastruloids

(2020) Susanne C. van den Brink et al.   NATURE

An in vitro model of early anteroposterior organization during human development

(2020) Naomi Moris et al.   NATURE

Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites

(2020) Jesse V. Veenvliet et al.   SCIENCE

High-resolution transcriptional and morphogenetic profiling of cells from micropatterned human ESC gastruloid cultures

(2020) Kyaw Thu Minn et al.   eLife

Capturing Cardiogenesis in Gastruloids

(2020) Giuliana Rossi et al.   Cell Stem Cell

Rapid changes in morphogen concentration control self-organized patterning in human embryonic stem cells

(2019) Idse Heemskerk et al.   eLife

Self-organizing neuruloids model developmental aspects of Huntington’s disease in the ectodermal compartment

(2019) Tomomi Haremaki et al.   NATURE BIOTECHNOLOGY

Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids

(2019) Sapna Chhabra et al.   PLOS BIOLOGY

High-throughput micropatterning platform reveals Nodal-dependent bisection of peri-gastrulation–associated versus preneurulation-associated fate patterning

(2019) Mukul Tewary et al.   PLOS BIOLOGY

Exposure-based assessment of chemical teratogenicity using morphogenetic aggregates of human embryonic stem cells

(2019) Yusuke Marikawa et al.   REPRODUCTIVE TOXICOLOGY

Mechanics-guided embryonic patterning of neuroectoderm tissue from human pluripotent stem cells

(2018) Xufeng Xue et al.   NATURE MATERIALS

Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning

(2018) Sophie M Morgani et al.   eLife

Self-organization of a human organizer by combined Wnt and Nodal signalling

(2018) I. Martyn et al.   NATURE

Multi-axial self-organization properties of mouse embryonic stem cells into gastruloids

(2018) Leonardo Beccari et al.   NATURE

A Balance between Secreted Inhibitors and Edge Sensing Controls Gastruloid Self-Organization

(2016) Fred Etoc et al.   DEVELOPMENTAL CELL

The Germ Cell Fate of Cynomolgus Monkeys Is Specified in the Nascent Amnion

(2016) Kotaro Sasaki et al.   DEVELOPMENTAL CELL

Exposure-Based Validation List for Developmental Toxicity Screening Assays

(2014) George P. Daston et al.   BIRTH DEFECTS RESEARCH PART B-DEVELOPMENTAL AND REPRODUCTIVE TOXICOLOGY

Symmetry breaking, germ layer specification and axial organisation in aggregates of mouse embryonic stem cells

(2014) S. C. van den Brink et al.   DEVELOPMENT

Wnt/ -catenin and FGF signalling direct the specification and maintenance of a neuromesodermal axial progenitor in ensembles of mouse embryonic stem cells

(2014) D. A. Turner et al.   DEVELOPMENT

A method to recapitulate early embryonic spatial patterning in human embryonic stem cells

(2014) Aryeh Warmflash et al.   NATURE METHODS

Aggregated P19 mouse embryonal carcinoma cells as a simple in vitro model to study the molecular regulations of mesoderm formation and axial elongation morphogenesis

(2009) Yusuke Marikawa et al.   GENESIS

Wnt Signaling Mediates Self-Organization and Axis Formation in Embryoid Bodies

(2008) Derk ten Berge et al.   Cell Stem Cell