Germline-targeting HIV-1 Env vaccination induces VRC01-class antibodies with rare insertions

Highly mutated antibodies capable of neutralizing N276 glycan-deficient HIV after a single immunization with an Env trimer

(2022) Jeong Hyun Lee et al.   Cell Reports

Vaccination induces maturation in a mouse model of diverse unmutated VRC01-class precursors to HIV-neutralizing antibodies with >50% breadth

(2021) Xuejun Chen et al.   IMMUNITY

Quadrivalent influenza nanoparticle vaccines induce broad protection

(2021) Seyhan Boyoglu-Barnum et al.   NATURE

Two Randomized Trials of Neutralizing Antibodies to Prevent HIV-1 Acquisition

(2021) Lawrence Corey et al.   NEW ENGLAND JOURNAL OF MEDICINE

Highly accurate protein structure prediction with AlphaFold

(2021) John Jumper et al.   NATURE

Vaccine genetics of IGHV1-2 VRC01-class broadly neutralizing antibody precursor naïve human B cells

(2021) Jeong Hyun Lee et al.   npj Vaccines

Restriction of HIV-1 Escape by a Highly Broad and Potent Neutralizing Antibody

(2020) Philipp Schommers et al.   CELL

Slow Delivery Immunization Enhances HIV Neutralizing Antibody and Germinal Center Responses via Modulation of Immunodominance

(2020) Kimberly M. Cirelli et al.   CELL

Env Exceptionalism: Why Are HIV-1 Env Glycoproteins Atypical Immunogens?

(2020) P.J. Klasse et al.   Cell Host & Microbe

Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability

(2020) Philip J. M. Brouwer et al.   SCIENCE

B cells expressing authentic naive human VRC01-class BCRs can be recruited to germinal centers and affinity mature in multiple independent mouse models

(2020) Deli Huang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mosaic nanoparticle display of diverse influenza virus hemagglutinins elicits broad B cell responses

(2019) Masaru Kanekiyo et al.   NATURE IMMUNOLOGY

Rapid and Focused Maturation of a VRC01-Class HIV Broadly Neutralizing Antibody Lineage Involves Both Binding and Accommodation of the N276-Glycan

(2019) Jeffrey Umotoy et al.   IMMUNITY

Neutralization-guided design of HIV-1 envelope trimers with high affinity for the unmutated common ancestor of CH235 lineage CD4bs broadly neutralizing antibodies

(2019) Celia C. LaBranche et al.   PLoS Pathogens

Enhancing and shaping the immunogenicity of native-like HIV-1 envelope trimers with a two-component protein nanoparticle

(2019) Philip J. M. Brouwer et al.   Nature Communications

The complex challenges of HIV vaccine development require renewed and expanded global commitment

(2019) Linda-Gail Bekker et al.   LANCET

Targeted selection of HIV-specific antibody mutations by engineering B cell maturation

(2019) Kevin O. Saunders et al.   SCIENCE

Identification of Near-Pan-neutralizing Antibodies against HIV-1 by Deconvolution of Plasma Humoral Responses

(2018) Mohammad Mohseni Sajadi et al.   CELL

Functional Relevance of Improbable Antibody Mutations for HIV Broadly Neutralizing Antibody Development

(2018) Kevin Wiehe et al.   Cell Host & Microbe

Precursor Frequency and Affinity Determine B Cell Competitive Fitness in Germinal Centers, Tested with Germline-Targeting HIV Vaccine Immunogens

(2018) Robert K. Abbott et al.   IMMUNITY

Integrity of Glycosylation Processing of a Glycan-Depleted Trimeric HIV-1 Immunogen Targeting Key B-Cell Lineages

(2018) Anna-Janina Behrens et al.   JOURNAL OF PROTEOME RESEARCH

Anti–HIV-1 B cell responses are dependent on B cell precursor frequency and antigen-binding affinity

(2018) Pia Dosenovic et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Recent progress in broadly neutralizing antibodies to HIV

(2018) Devin Sok et al.   NATURE IMMUNOLOGY

HIV-1 envelope glycan modifications that permit neutralization by germline-reverted VRC01-class broadly neutralizing antibodies

(2018) Celia C. LaBranche et al.   PLoS Pathogens

Genetic and structural analyses of affinity maturation in the humoral response to HIV-1

(2017) Thomas B. Kepler et al.   IMMUNOLOGICAL REVIEWS

Human Ig knockin mice to study the development and regulation of HIV-1 broadly neutralizing antibodies

(2017) Laurent Verkoczy et al.   IMMUNOLOGICAL REVIEWS

Germline-targeting immunogens

(2017) Leonidas Stamatatos et al.   IMMUNOLOGICAL REVIEWS

Design and crystal structure of a native-like HIV-1 envelope trimer that engages multiple broadly neutralizing antibody precursors in vivo

(2017) Max Medina-Ramírez et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Protection against a mixed SHIV challenge by a broadly neutralizing antibody cocktail

(2017) Boris Julg et al.   Science Translational Medicine

Initiation of HIV neutralizing B cell lineages with sequential envelope immunizations

(2017) Wilton B. Williams et al.   Nature Communications

Improving the Immunogenicity of Native-like HIV-1 Envelope Trimers by Hyperstabilization

(2017) Alba Torrents de la Peña et al.   Cell Reports

Strategies for carbohydrate model building, refinement and validation

(2017) Jon Agirre   Acta Crystallographica Section D-Structural Biology

Tailored Immunogens Direct Affinity Maturation toward HIV Neutralizing Antibodies

(2016) Bryan Briney et al.   CELL

Sequential Immunization Elicits Broadly Neutralizing Anti-HIV-1 Antibodies in Ig Knockin Mice

(2016) Amelia Escolano et al.   CELL

Induction of HIV Neutralizing Antibody Lineages in Mice with Diverse Precursor Repertoires

(2016) Ming Tian et al.   CELL

HIV Vaccine Design to Target Germline Precursors of Glycan-Dependent Broadly Neutralizing Antibodies

(2016) Jon M. Steichen et al.   IMMUNITY

Key gp120 Glycans Pose Roadblocks to the Rapid Development of VRC01-Class Antibodies in an HIV-1-Infected Chinese Donor

(2016) Leopold Kong et al.   IMMUNITY

Identification of a CD4-Binding-Site Antibody to HIV that Evolved Near-Pan Neutralization Breadth

(2016) Jinghe Huang et al.   IMMUNITY

HIV-1 antibody 3BNC117 suppresses viral rebound in humans during treatment interruption

(2016) Johannes F. Scheid et al.   NATURE

HIV-1 broadly neutralizing antibody precursor B cells revealed by germline-targeting immunogen

(2016) J. G. Jardine et al.   SCIENCE

Accurate design of megadalton-scale two-component icosahedral protein complexes

(2016) J. B. Bale et al.   SCIENCE

Initiation of immune tolerance–controlled HIV gp41 neutralizing B cell lineages

(2016) Ruijun Zhang et al.   Science Translational Medicine

Specifically modified Env immunogens activate B-cell precursors of broadly neutralizing HIV-1 antibodies in transgenic mice

(2016) Andrew T. McGuire et al.   Nature Communications

Minimally Mutated HIV-1 Broadly Neutralizing Antibodies to Guide Reductionist Vaccine Design

(2016) Joseph G. Jardine et al.   PLoS Pathogens

Immunogenicity of Stabilized HIV-1 Envelope Trimers with Reduced Exposure of Non-neutralizing Epitopes

(2015) Steven W. de Taeye et al.   CELL

Immunization for HIV-1 Broadly Neutralizing Antibodies in Human Ig Knockin Mice

(2015) Pia Dosenovic et al.   CELL

Design and structure of two HIV-1 clade C SOSIP.664 trimers that increase the arsenal of native-like Env immunogens

(2015) Jean-Philippe Julien et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Priming a broadly neutralizing antibody response to HIV-1 using a germline-targeting immunogen

(2015) J. G. Jardine et al.   SCIENCE

Binding of inferred germline precursors of broadly neutralizing HIV-1 antibodies to native-like envelope trimers

(2015) Kwinten Sliepen et al.   VIROLOGY

Immunoglobulin Gene Insertions and Deletions in the Affinity Maturation of HIV-1 Broadly Reactive Neutralizing Antibodies

(2014) Thomas B. Kepler et al.   Cell Host & Microbe

Targeting B-cell germlines and focusing affinity maturation: the next hurdles in HIV-1-vaccine development?

(2014) Max Medina-Ramírez et al.   Expert Review of Vaccines

Differential binding of neutralizing and non-neutralizing antibodies to native-like soluble HIV-1 Env trimers, uncleaved Env proteins, and monomeric subunits

(2014) Anila Yasmeen et al.   Retrovirology

Antigen modification regulates competition of broad and narrow neutralizing HIV antibodies

(2014) A. T. McGuire et al.   SCIENCE

Reconstructing a B-Cell Clonal Lineage. II. Mutation, Selection, and Affinity Maturation

(2014) Thomas B. Kepler et al.   Frontiers in Immunology

Prevalence of broadly neutralizing antibody responses during chronic HIV-1 infection

(2013) Peter Hraber et al.   AIDS

Common Tolerance Mechanisms, but Distinct Cross-Reactivities Associated with gp41 and Lipids, Limit Production of HIV-1 Broad Neutralizing Antibodies 2F5 and 4E10

(2013) Y. Chen et al.   JOURNAL OF IMMUNOLOGY

Diverse Recombinant HIV-1 Envs Fail To Activate B Cells Expressing the Germline B Cell Receptors of the Broadly Neutralizing Anti-HIV-1 Antibodies PG9 and 447-52D

(2013) A. T. McGuire et al.   JOURNAL OF VIROLOGY

Co-evolution of a broadly neutralizing HIV-1 antibody and founder virus

(2013) Hua-Xin Liao et al.   NATURE

Recombinant HIV Envelope Proteins Fail to Engage Germline Versions of Anti-CD4bs bNAbs

(2013) Sam Hoot et al.   PLoS Pathogens

A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies

(2013) Rogier W. Sanders et al.   PLoS Pathogens

Models of Somatic Hypermutation Targeting and Substitution Based on Synonymous Mutations from High-Throughput Immunoglobulin Sequencing Data

(2013) Gur Yaari et al.   Frontiers in Immunology

Two Distinct Broadly Neutralizing Antibody Specificities of Different Clonal Lineages in a Single HIV-1-Infected Donor: Implications for Vaccine Design

(2012) Mattia Bonsignori et al.   JOURNAL OF VIROLOGY

B-cell–lineage immunogen design in vaccine development with HIV-1 as a case study

(2012) Barton F Haynes et al.   NATURE BIOTECHNOLOGY

FLASH: fast length adjustment of short reads to improve genome assemblies

(2011) T. Magoc et al.   BIOINFORMATICS

Rescue of HIV-1 Broad Neutralizing Antibody-Expressing B Cells in 2F5 VH x VL Knockin Mice Reveals Multiple Tolerance Controls

(2011) L. Verkoczy et al.   JOURNAL OF IMMUNOLOGY

Sequence and Structural Convergence of Broad and Potent HIV Antibodies That Mimic CD4 Binding

(2011) J. F. Scheid et al.   SCIENCE

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

The JCSG high-throughput structural biology pipeline

(2010) Marc-André Elsliger et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

Structural Basis for Broad and Potent Neutralization of HIV-1 by Antibody VRC01

(2010) T. Zhou et al.   SCIENCE

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Germline-like predecessors of broadly neutralizing antibodies lack measurable binding to HIV-1 envelope glycoproteins: Implications for evasion of immune responses and design of vaccine immunogens

(2009) Xiaodong Xiao et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Cloning and expression of murine Ig genes from single B cells

(2009) Thomas Tiller et al.   JOURNAL OF IMMUNOLOGICAL METHODS

DoG Picker and TiltPicker: Software tools to facilitate particle selection in single particle electron microscopy

(2009) N.R. Voss et al.   JOURNAL OF STRUCTURAL BIOLOGY

Breadth of Human Immunodeficiency Virus-Specific Neutralizing Activity in Sera: Clustering Analysis and Association with Clinical Variables

(2009) N. A. Doria-Rose et al.   JOURNAL OF VIROLOGY

Autoreactivity in an HIV-1 broadly reactive neutralizing antibody variable region heavy chain induces immunologic tolerance

(2009) L. Verkoczy et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Optimization of Human Immunodeficiency Virus Type 1 Envelope Glycoproteins with V1/V2 Deleted, Using Virus Evolution

(2008) I. Bontjer et al.   JOURNAL OF VIROLOGY

Challenges in the development of an HIV-1 vaccine

(2008) Dan H. Barouch   NATURE