Cell-free eukaryotic systems for the production, engineering, and modification of scFv antibody fragments

Synthesis and site-directed fluorescence labeling of azido proteins using eukaryotic cell-free orthogonal translation systems

(2014) Robert B. Quast et al.   ANALYTICAL BIOCHEMISTRY

Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System

(2014) Erik S. Zimmerman et al.   BIOCONJUGATE CHEMISTRY

Membrane assembly of the functional KcsA potassium channel in a vesicle-based eukaryotic cell-free translation system

(2014) Srujan Kumar Dondapati et al.   BIOSENSORS & BIOELECTRONICS

A Continuous-Exchange Cell-Free Protein Synthesis System Based on Extracts from Cultured Insect Cells

(2014) Marlitt Stech et al.   PLoS One

Cell-free synthesis of membrane proteins: Tailored cell models out of microsomes

(2013) Susanne F. Fenz et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

IRES-Mediated Translation of Membrane Proteins and Glycoproteins in Eukaryotic Cell-Free Systems

(2013) Andreas K. Brödel et al.   PLoS One

Synthesis of membrane proteins in eukaryotic cell-free systems

(2012) Rita Sachse et al.   ENGINEERING IN LIFE SCIENCES

Functional evaluation of candidate ice structuring proteins using cell-free expression systems

(2012) A.K. Brödel et al.   JOURNAL OF BIOTECHNOLOGY

Production of functional antibody fragments in a vesicle-based eukaryotic cell-free translation system

(2012) Marlitt Stech et al.   JOURNAL OF BIOTECHNOLOGY

Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system

(2012) Gang Yin et al.   mAbs

Synthesis of site-specific antibody-drug conjugates using unnatural amino acids

(2012) J. Y. Axup et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

Efficient disulfide bond formation in virus-like particles

(2011) Bradley C. Bundy et al.   JOURNAL OF BIOTECHNOLOGY

A roadmap to generate renewable protein binders to the human proteome

(2011) Karen Colwill et al.   NATURE METHODS

A human scFv antibody generation pipeline for proteome research

(2010) Michael Hust et al.   JOURNAL OF BIOTECHNOLOGY

Escherichia coli-based production of a tumor idiotype antibody fragment – tetanus toxin fragment C fusion protein vaccine for B cell lymphoma

(2010) Kedar G. Patel et al.   PROTEIN EXPRESSION AND PURIFICATION

Cell-free production of functional antibody fragments

(2009) In-Seok Oh et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Applications of single-chain variable fragment antibodies in therapeutics and diagnostics

(2009) Nina E. Weisser et al.   BIOTECHNOLOGY ADVANCES

Regulation of Translation Initiation in Eukaryotes: Mechanisms and Biological Targets

(2009) Nahum Sonenberg et al.   CELL

Protein Incorporation in Giant Lipid Vesicles under Physiological Conditions

(2009) Paige M. Shaklee et al.   CHEMBIOCHEM

Improved microtitre plate production of single chain Fv fragments in Escherichia coli

(2009) Michael Hust et al.   New Biotechnology