Cotranslational incorporation of non-standard amino acids using cell-free protein synthesis

Bioorthogonal Reactions for Labeling Proteins

(2014) Kathrin Lang et al.   ACS Chemical Biology

Synthesis and site-directed fluorescence labeling of azido proteins using eukaryotic cell-free orthogonal translation systems

(2014) Robert B. Quast et al.   ANALYTICAL BIOCHEMISTRY

Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System

(2014) Erik S. Zimmerman et al.   BIOCONJUGATE CHEMISTRY

Membrane assembly of the functional KcsA potassium channel in a vesicle-based eukaryotic cell-free translation system

(2014) Srujan Kumar Dondapati et al.   BIOSENSORS & BIOELECTRONICS

Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system

(2014) Matthias Buntru et al.   BMC BIOTECHNOLOGY

Cell-free eukaryotic systems for the production, engineering, and modification of scFv antibody fragments

(2014) Marlitt Stech et al.   ENGINEERING IN LIFE SCIENCES

Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes

(2014) Rita Sachse et al.   FEBS LETTERS

Wheat germ systems for cell-free protein expression

(2014) Matthias Harbers   FEBS LETTERS

Efficient Multisite Unnatural Amino Acid Incorporation in Mammalian Cells via Optimized Pyrrolysyl tRNA Synthetase/tRNA Expression and Engineered eRF1

(2014) Wolfgang H. Schmied et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Characterizing IGR IRES-mediated translation initiation for use in yeast cell-free protein synthesis

(2014) C. Eric Hodgman et al.   New Biotechnology

A Continuous-Exchange Cell-Free Protein Synthesis System Based on Extracts from Cultured Insect Cells

(2014) Marlitt Stech et al.   PLoS One

A Cell-Free Translocation System Using Extracts of Cultured Insect Cells to Yield Functional Membrane Proteins

(2014) Toru Ezure et al.   PLoS One

Ultra Q-bodies: quench-based antibody probes that utilize dye-dye interactions with enhanced antigen-dependent fluorescence

(2014) Ryoji Abe et al.   Scientific Reports

Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis

(2014) Seok Hoon Hong et al.   Frontiers in Chemistry

Using E. coli-based cell-free protein synthesis to evaluate the kinetic performance of an orthogonal tRNA and aminoacyl-tRNA synthetase pair

(2013) Cem Albayrak et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis

(2013) C. Eric Hodgman et al.   BIOTECHNOLOGY AND BIOENGINEERING

Amino Acid-Selective Segmental Isotope Labeling of Multidomain Proteins for Structural Biology

(2013) Erich Michel et al.   CHEMBIOCHEM

Cell-free translation of peptides and proteins:from high throughput screening to clinical production

(2013) Christopher J Murray et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Advances in NMR structures of integral membrane proteins

(2013) Innokentiy Maslennikov et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Experimental challenges of sense codon reassignment: An innovative approach to genetic code expansion

(2013) Radha Krishnakumar et al.   FEBS LETTERS

Engine out of the chassis: Cell-free protein synthesis and its uses

(2013) Gabriel Rosenblum et al.   FEBS LETTERS

A Kinetic Safety Gate Controlling the Delivery of Unnatural Amino Acids to the Ribosome

(2013) Joerg Mittelstaet et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Optimized Phospholipid Bilayer Nanodiscs Facilitate High-Resolution Structure Determination of Membrane Proteins

(2013) Franz Hagn et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Upgrading protein synthesis for synthetic biology

(2013) Patrick O'Donoghue et al.   Nature Chemical Biology

Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation

(2013) Cem Albayrak et al.   NUCLEIC ACIDS RESEARCH

IRES-Mediated Translation of Membrane Proteins and Glycoproteins in Eukaryotic Cell-Free Systems

(2013) Andreas K. Brödel et al.   PLoS One

The next level of complexity: Crosstalk of posttranslational modifications

(2013) A. Saskia Venne et al.   PROTEOMICS

Cell-free Protein Synthesis from a Release Factor 1 Deficient Escherichia coli Activates Efficient and Multiple Site-specific Nonstandard Amino Acid Incorporation

(2013) Seok Hoon Hong et al.   ACS Synthetic Biology

Multiple-Site Labeling of Proteins with Unnatural Amino Acids

(2012) Karin V. Loscha et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

High-yield cell-free protein synthesis for site-specific incorporation of unnatural amino acids at two sites

(2012) Kiyoshi Ozawa et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Structural Diversity and Protein Engineering of the Aminoacyl-tRNA Synthetases

(2012) John J. Perona et al.   BIOCHEMISTRY

Purification and visualization of encephalomyocarditisvirus synthesized by an in vitro protein expression system derived from mammalian cell extract

(2012) Tominari Kobayashi et al.   BIOTECHNOLOGY LETTERS

Enhanced protein stability through minimally invasive, direct, covalent, and site-specific immobilization

(2012) Mark T. Smith et al.   BIOTECHNOLOGY PROGRESS

Synthesis of membrane proteins in eukaryotic cell-free systems

(2012) Rita Sachse et al.   ENGINEERING IN LIFE SCIENCES

Cell-free expression of disulfide-containing eukaryotic proteins for structural biology

(2012) Erich Michel et al.   FEBS Journal

Near-cognate suppression of amber, opal and quadruplet codons competes with aminoacyl-tRNAPylfor genetic code expansion

(2012) Patrick O'Donoghue et al.   FEBS LETTERS

The protease-activated receptor 1 possesses a functional and cleavable signal peptide which is necessary for receptor expression

(2012) Dimitris E. Zampatis et al.   FEBS LETTERS

Facile backbone structure determination of human membrane proteins by NMR spectroscopy

(2012) Christian Klammt et al.   NATURE METHODS

Site specific chemoselective labelling of proteins with robust and highly sensitive Ru(ii) bathophenanthroline complexes

(2012) Matthew C. Uzagare et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Performance Analysis of Orthogonal Pairs Designed for an Expanded Eukaryotic Genetic Code

(2012) Sebastian Nehring et al.   PLoS One

Unnatural base pair systems toward the expansion of the genetic alphabet in the central dogma

(2012) Ichiro HIRAO et al.   PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES

Biotinylated Rh(III) Complexes in Engineered Streptavidin for Accelerated Asymmetric C-H Activation

(2012) T. K. Hyster et al.   SCIENCE

Synthesis and in situ insertion of a site-specific fluorescently labeled membrane protein into cell-sized liposomes

(2011) Takuma Ohtsuka et al.   ANALYTICAL BIOCHEMISTRY

Solution NMR Structure of Proteorhodopsin

(2011) Sina Reckel et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Genetic-code evolution for protein synthesis with non-natural amino acids

(2011) Takahito Mukai et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Surface Functionalization of Virus-Like Particles by Direct Conjugation Using Azide−Alkyne Click Chemistry

(2011) Kedar G. Patel et al.   BIOCONJUGATE CHEMISTRY

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Leishmania cell-free protein expression system

(2011) Oleksiy Kovtun et al.   METHODS

An efficient factor-depleted mammalian in vitro translation system

(2011) Aurélie M Rakotondrafara et al.   Nature Protocols

Generation of Pseudocontact Shifts in Protein NMR Spectra with a Genetically Encoded Cobalt(II)-Binding Amino Acid

(2010) Thi Hoang Duong Nguyen et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

Using a Genetically Encoded Fluorescent Amino Acid as a Site-Specific Probe to Detect Binding of Low-Molecular-Weight Compounds

(2010) Isaac N. Ugwumba et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Site-Specific Incorporation ofp-Propargyloxyphenylalanine in a Cell-Free Environment for Direct Protein−Protein Click Conjugation

(2010) Bradley C. Bundy et al.   BIOCONJUGATE CHEMISTRY

Residue-specific incorporation of non-canonical amino acids into proteins: recent developments and applications

(2010) Jeremiah A Johnson et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Site-specific incorporation of 4-Iodo-l-phenylalanine through opal suppression

(2010) Koichiro Kodama et al.   JOURNAL OF BIOCHEMISTRY

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome

(2010) Heinz Neumann et al.   NATURE

Practical cell-free protein synthesis system using purified wheat embryos

(2010) Kazuyuki Takai et al.   Nature Protocols

Evolved orthogonal ribosome purification for in vitro characterization

(2010) Oliver P. T. Barrett et al.   NUCLEIC ACIDS RESEARCH

Membrane domain structures of three classes of histidine kinase receptors by cell-free expression and rapid NMR analysis

(2010) I. Maslennikov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Site-specific PEGylation of proteins by a Staudinger-phosphite reaction

(2010) Remigiusz Serwa et al.   Chemical Science

A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates

(2009) Hidetaka Nakata et al.   ANALYTICAL BIOCHEMISTRY

Chemoselective Staudinger-Phosphite Reaction of Azides for the Phosphorylation of Proteins

(2009) Remigiusz Serwa et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cell-free production of Gaussia princeps luciferase – antibody fragment bioconjugates for ex vivo detection of tumor cells

(2009) Kedar G. Patel et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Multiply mutated Gaussia luciferases provide prolonged and intense bioluminescence

(2009) John P. Welsh et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Protein Incorporation in Giant Lipid Vesicles under Physiological Conditions

(2009) Paige M. Shaklee et al.   CHEMBIOCHEM

Position-Specific Incorporation of Fluorescent Non-natural Amino Acids into Maltose-Binding Protein for Detection of Ligand Binding by FRET and Fluorescence Quenching

(2009) Issei Iijima et al.   CHEMBIOCHEM

Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells

(2009) Jeffrey K. Takimoto et al.   Molecular BioSystems

Optimization of the hybridization-based method for purification of thermostable tRNAs in the presence of tetraalkylammonium salts

(2009) Takashi Yokogawa et al.   NUCLEIC ACIDS RESEARCH

Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases

(2008) Takahito Mukai et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

High-level cell-free synthesis yields of proteins containing site-specific non-natural amino acids

(2008) Aaron R. Goerke et al.   BIOTECHNOLOGY AND BIOENGINEERING

Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode Nɛ-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification

(2008) Tatsuo Yanagisawa et al.   CHEMISTRY & BIOLOGY

Recognition of Non-α-amino Substrates by Pyrrolysyl-tRNA Synthetase

(2008) Takatsugu Kobayashi et al.   JOURNAL OF MOLECULAR BIOLOGY