SNARE assembly enlightened by cryo-EM structures of a synaptobrevin–Munc18-1–syntaxin-1 complex

Molecular Mechanisms Underlying Neurotransmitter Release

(2022) Josep Rizo   Annual Review of Biophysics

Phosphatidylinositol and phosphatidylinositol-3-phosphate activate HOPS to catalyze SNARE assembly, allowing small headgroup lipids to support the terminal steps of membrane fusion

(2021) Thomas Torng et al.   MOLECULAR BIOLOGY OF THE CELL

Control of neurotransmitter release by two distinct membrane-binding faces of the Munc13-1 C1C2B region

(2021) Marcial Camacho et al.   eLife

Re‐examining how Munc13‐1 facilitates opening of syntaxin‐1

(2020) Magdalena Magdziarek et al.   PROTEIN SCIENCE

Asymmetric Rab activation of vacuolar HOPS to catalyze SNARE complex assembly

(2020) Thomas Torng et al.   MOLECULAR BIOLOGY OF THE CELL

Munc13 activates the Munc18‐1/syntaxin‐1 complex and enables Munc18‐1 to prime SNARE assembly

(2020) Xianping Wang et al.   EMBO JOURNAL

The Sec1/Munc18 protein Vps45 holds the Qa-SNARE Tlg2 in an open conformation

(2020) Travis J Eisemann et al.   eLife

The Interaction of Munc18-1 Helix 11 and 12 with the Central Region of the VAMP2 SNARE Motif Is Essential for SNARE Templating and Synaptic Transmission

(2020) Timon André et al.   eNeuro

Multiple factors maintain assembled trans-SNARE complexes in the presence of NSF and aSNAP

(2019) Eric A Prinslow et al.   eLife

Membrane bridging by Munc13-1 is crucial for neurotransmitter release

(2019) Bradley Quade et al.   eLife

Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix

(2019) Dorothee Liebschner et al.   Acta Crystallographica Section D-Structural Biology

Munc18-1 is crucial to overcome the inhibition of synaptic vesicle fusion by αSNAP

(2019) Karolina P. Stepien et al.   Nature Communications

Quantitative mass imaging of single biological macromolecules

(2018) Gavin Young et al.   SCIENCE

New tools for automated high-resolution cryo-EM structure determination in RELION-3

(2018) Jasenko Zivanov et al.   eLife

Munc18-1 catalyzes neuronal SNARE assembly by templating SNARE association

(2018) Junyi Jiao et al.   eLife

UNC-18 and Tomosyn Antagonistically Control Synaptic Vesicle Priming Downstream of UNC-13 inCaenorhabditis elegans

(2017) Seungmee Park et al.   JOURNAL OF NEUROSCIENCE

A short region upstream of the yeast vacuolar Qa-SNARE heptad-repeats promotes membrane fusion through enhanced SNARE complex assembly

(2017) Hongki Song et al.   MOLECULAR BIOLOGY OF THE CELL

Simultaneous lipid and content mixing assays for in vitro reconstitution studies of synaptic vesicle fusion

(2017) Xiaoxia Liu et al.   Nature Protocols

Autoinhibition of Munc18-1 modulates synaptobrevin binding and helps to enable Munc13-dependent regulation of membrane fusion

(2017) Ewa Sitarska et al.   eLife

Mechanistic insights into neurotransmitter release and presynaptic plasticity from the crystal structure of Munc13-1 C1C2BMUN

(2017) Junjie Xu et al.   eLife

Extension of Helix 12 in Munc18-1 Induces Vesicle Priming

(2016) A. S. Munch et al.   JOURNAL OF NEUROSCIENCE

Functional synergy between the Munc13 C-terminal C1 and C2 domains

(2016) Xiaoxia Liu et al.   eLife

Syntaxin opening by the MUN domain underlies the function of Munc13 in synaptic-vesicle priming

(2015) Xiaoyu Yang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly

(2015) R. W. Baker et al.   SCIENCE

Syntaxin opening by the MUN domain underlies the function of Munc13 in synaptic-vesicle priming

(2015) Xiaoyu Yang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Munc18-1-regulated stage-wise SNARE assembly underlying synaptic exocytosis

(2015) Lu Ma et al.   eLife

An Extended Helical Conformation in Domain 3a of Munc18-1 Provides a Template for SNARE (SolubleN-Ethylmaleimide-sensitive Factor Attachment Protein Receptor) Complex Assembly

(2014) Daniel Parisotto et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Pivotal Role for Pro-335 in Balancing the Dual Functions of Munc18-1 Domain-3a in Regulated Exocytosis

(2014) Gayoung Anna Han et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Neurotransmitter Release: The Last Millisecond in the Life of a Synaptic Vesicle

(2013) Thomas C. Südhof   NEURON

Syntaxin1a variants lacking an N-peptide or bearing the LE mutation bind to Munc18a in a closed conformation

(2013) K. N. Colbert et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reconstitution of the Vital Functions of Munc18 and Munc13 in Neurotransmitter Release

(2012) C. Ma et al.   SCIENCE

Munc13 mediates the transition from the closed syntaxin–Munc18 complex to the SNARE complex

(2011) Cong Ma et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

SNARE Proteins Underpin Insulin-Regulated GLUT4 Traffic

(2011) Nia J. Bryant et al.   TRAFFIC

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Binding of Munc18-1 to Synaptobrevin and to the SNARE Four-Helix Bundle

(2010) Yi Xu et al.   BIOCHEMISTRY

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Munc18-2 deficiency causes familial hemophagocytic lymphohistiocytosis type 5 and impairs cytotoxic granule exocytosis in patient NK cells

(2009) Marjorie Côte et al.   JOURNAL OF CLINICAL INVESTIGATION

Munc18a controls SNARE assembly through its interaction with the syntaxin N-peptide

(2008) Pawel Burkhardt et al.   EMBO JOURNAL