Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains

The Landscape of Recombination Events That Create Nonribosomal Peptide Diversity

(2021) Martin Baunach et al.   MOLECULAR BIOLOGY AND EVOLUTION

Engineering and elucidation of the lipoinitiation process in nonribosomal peptide biosynthesis

(2021) Lin Zhong et al.   Nature Communications

Structural characterization of a PCP–R di-domain from an archaeal non-ribosomal peptide synthetase reveals novel inter-domain interactions

(2021) Sandesh Deshpande et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Evolutionary and functional analysis of an NRPS condensation domain integrates β-lactam, ᴅ-amino acid, and dehydroamino acid synthesis

(2021) Michael J. Wheadon et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structures of a non-ribosomal peptide synthetase condensation domain suggest the basis of substrate selectivity

(2021) Thierry Izoré et al.   Nature Communications

Elucidation of transient protein-protein interactions within carrier protein-dependent biosynthesis

(2021) Thomas G. Bartholow et al.   Communications Biology

Engineered Nonribosomal Peptide Synthetase Shows Opposite Amino Acid Loading and Condensation Specificity

(2021) Aleksa Stanišić et al.   ACS Catalysis

Precise Probing of Residue Roles by NRPS Code Swapping: Mutation, Enzymatic Characterization, Modeling, and Substrate Promiscuity of Aryl Acid Adenylation Domains

(2020) Fumihiro Ishikawa et al.   BIOCHEMISTRY

Conformational flexibility of coenzyme A and its impact on the post‐translational modification of acyl carrier proteins by 4’‐phosphopantetheinyl transferases

(2020) Minh Chau Nguyen et al.   FEBS Journal

Design, Synthesis, and Biophysical Evaluation of Mechanism-Based Probes for Condensation Domains of Nonribosomal Peptide Synthetases

(2020) Ce Shi et al.   ACS Chemical Biology

Structural Characterization of Complex of Adenylation Domain and Carrier Protein by Using Pantetheine Cross-Linking Probe

(2020) Akimasa Miyanaga et al.   ACS Chemical Biology

Redesign of Substrate Selection in Glycopeptide Antibiotic Biosynthesis Enables Effective Formation of Alternate Peptide Backbones

(2020) Milda Kaniusaite et al.   ACS Chemical Biology

Efficient rational modification of non-ribosomal peptides by adenylation domain substitution

(2020) Mark J. Calcott et al.   Nature Communications

Insights into thiotemplated pyrrole biosynthesis gained by crystal structure of flavin-dependent oxidase in complex with carrier protein

(2019) Hem R Thapa et al.   BIOCHEMISTRY

Targeting adenylate-forming enzymes with designed sulfonyladenosine inhibitors

(2019) Michaelyn C. Lux et al.   JOURNAL OF ANTIBIOTICS

Modification and de novo design of non-ribosomal peptide synthetases using specific assembly points within condensation domains

(2019) Kenan A. J. Bozhüyük et al.   Nature Chemistry

The structural basis of N-acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase

(2019) Dale F. Kreitler et al.   Nature Communications

Probing and Engineering the Fatty Acyl Substrate Selectivity of Starter Condensation Domains of Nonribosomal Peptide Synthetases in Lipopeptide Biosynthesis

(2019) Qian Liu et al.   Biotechnology Journal

Structural basis for chain release from the enacyloxin polyketide synthase

(2019) Simone Kosol et al.   Nature Chemistry

Molecular Mechanism for Gramicidin Dimerization and Dissociation in Bilayers of Different Thickness

(2019) Delin Sun et al.   BIOPHYSICAL JOURNAL

Structures of a dimodular nonribosomal peptide synthetase reveal conformational flexibility

(2019) Janice M. Reimer et al.   SCIENCE

Reprogramming Nonribosomal Peptide Synthesis by Surgical Mutation

(2019) David L. Niquille et al.   SYNLETT

Mechanistic Probes for the Epimerization Domain of Nonribosomal Peptide Synthetases

(2018) Woojoo Kim et al.   CHEMBIOCHEM

Structural, functional and evolutionary perspectives on effective re-engineering of non-ribosomal peptide synthetase assembly lines

(2018) Alistair S. Brown et al.   NATURAL PRODUCT REPORTS

Type II fatty acid and polyketide synthases: deciphering protein–protein and protein–substrate interactions

(2018) Aochiu Chen et al.   NATURAL PRODUCT REPORTS

Protein–protein interactions in polyketide synthase–nonribosomal peptide synthetase hybrid assembly lines

(2018) Akimasa Miyanaga et al.   NATURAL PRODUCT REPORTS

Trapping interactions between catalytic domains and carrier proteins of modular biosynthetic enzymes with chemical probes

(2018) Andrew M. Gulick et al.   NATURAL PRODUCT REPORTS

The many faces and important roles of protein–protein interactions during non-ribosomal peptide synthesis

(2018) Thierry Izoré et al.   NATURAL PRODUCT REPORTS

Discovering de novo peptide substrates for enzymes using machine learning

(2018) Lorillee Tallorin et al.   Nature Communications

FRET monitoring of a nonribosomal peptide synthetase

(2017) Jonas Alfermann et al.   Nature Chemical Biology

Structures of carboxylic acid reductase reveal domain dynamics underlying catalysis

(2017) Deepankar Gahloth et al.   Nature Chemical Biology

De novo design and engineering of non-ribosomal peptide synthetases

(2017) Kenan A. J. Bozhüyük et al.   Nature Chemistry

Interdomain and Intermodule Organization in Epimerization Domain Containing Nonribosomal Peptide Synthetases

(2016) Wei-Hung Chen et al.   ACS Chemical Biology

Designed Small-Molecule Inhibitors of the Anthranilyl-CoA Synthetase PqsA Block Quinolone Biosynthesis in Pseudomonas aeruginosa

(2016) Cheng Ji et al.   ACS Chemical Biology

Recent advances in engineering nonribosomal peptide assembly lines

(2016) M. Winn et al.   NATURAL PRODUCT REPORTS

Polyketide synthase and non-ribosomal peptide synthetase thioesterase selectivity: logic gate or a victim of fate?

(2016) Mark E. Horsman et al.   NATURAL PRODUCT REPORTS

Structures of two distinct conformations of holo-non-ribosomal peptide synthetases

(2016) Eric J. Drake et al.   NATURE

Synthetic cycle of the initiation module of a formylating nonribosomal peptide synthetase

(2016) Janice M. Reimer et al.   NATURE

Structural basis of nonribosomal peptide macrocyclization in fungi

(2016) Jinru Zhang et al.   Nature Chemical Biology

Structure and Substrate Sequestration in the Pyoluteorin Type II Peptidyl Carrier Protein PltL

(2015) Matt J. Jaremko et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The Structure of a Transient Complex of a Nonribosomal Peptide Synthetase and a Cytochrome P450 Monooxygenase

(2014) Kristina Haslinger et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Roles of type II thioesterases and their application for secondary metabolite yield improvement

(2014) Magdalena Kotowska et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Crystal Structure of a PCP/Sfp Complex Reveals the Structural Basis for Carrier Protein Posttranslational Modification

(2014) Peter Tufar et al.   CHEMISTRY & BIOLOGY

Analysis of the linker region joining the adenylation and carrier protein domains of the modular nonribosomal peptide synthetases

(2014) Bradley R. Miller et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Crystal Structures of the First Condensation Domain of CDA Synthetase Suggest Conformational Changes during the Synthetic Cycle of Nonribosomal Peptide Synthetases

(2013) Kristjan Bloudoff et al.   JOURNAL OF MOLECULAR BIOLOGY

The phosphopantetheinyl transferases: catalysis of a post-translational modification crucial for life

(2013) Joris Beld et al.   NATURAL PRODUCT REPORTS

Structure of PA1221, a Nonribosomal Peptide Synthetase Containing Adenylation and Peptidyl Carrier Protein Domains

(2012) Carter A. Mitchell et al.   BIOCHEMISTRY

Structural and Functional Investigation of the Intermolecular Interaction between NRPS Adenylation and Carrier Protein Domains

(2012) Jesse A. Sundlov et al.   CHEMISTRY & BIOLOGY

Explorations of catalytic domains in non-ribosomal peptide synthetase enzymology

(2012) Gene H. Hur et al.   NATURAL PRODUCT REPORTS

Structural Basis for Phosphopantetheinyl Carrier Domain Interactions in the Terminal Module of Nonribosomal Peptide Synthetases

(2011) Ye Liu et al.   CHEMISTRY & BIOLOGY

Designed Semisynthetic Protein Inhibitors of Ub/Ubl E1 Activating Enzymes

(2010) Xuequan Lu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Active site remodelling accompanies thioester bond formation in the SUMO E1

(2010) Shaun K. Olsen et al.   NATURE

Conformational Dynamics in the Acyl-CoA Synthetases, Adenylation Domains of Non-ribosomal Peptide Synthetases, and Firefly Luciferase

(2009) Andrew M. Gulick   ACS Chemical Biology

The Mechanism of Domain Alternation in the Acyl-Adenylate Forming Ligase Superfamily Member 4-Chlorobenzoate: Coenzyme A Ligase,

(2009) Rui Wu et al.   BIOCHEMISTRY

Mechanism-based inhibitors of MenE, an acyl-CoA synthetase involved in bacterial menaquinone biosynthesis

(2008) Xuequan Lu et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Crystal Structure of DltA

(2008) Huma Yonus et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural basis for the selectivity of the external thioesterase of the surfactin synthetase

(2008) Alexander Koglin et al.   NATURE

Crystal Structure of the Termination Module of a Nonribosomal Peptide Synthetase

(2008) A. Tanovic et al.   SCIENCE