Synthetic developmental biology: Engineering approaches to guide multicellular organization

Revealing Tissue-Specific SARS-CoV-2 Infection and Host Responses using Human Stem Cell-Derived Lung and Cerebral Organoids

(2021) Shashi Kant Tiwari et al.   Stem Cell Reports

Novel synthetic biology approaches for developmental systems

(2021) Christine Ho et al.   Stem Cell Reports

From embryos to embryoids: How external signals and self-organization drive embryonic development

(2021) J. Serrano Morales et al.   Stem Cell Reports

Self-organized signaling in stem cell models of embryos

(2021) Lizhong Liu et al.   Stem Cell Reports

Bioengineering in vitro models of embryonic development

(2021) Ananya Gupta et al.   Stem Cell Reports

Tissue-Specific Microparticles Improve Organoid Microenvironment for Efficient Maturation of Pluripotent Stem-Cell-Derived Hepatocytes

(2021) Ensieh Zahmatkesh et al.   Cells

Relaxation of Extracellular Matrix Forces Directs Crypt Formation and Architecture in Intestinal Organoids

(2020) Ella A. Hushka et al.   Advanced Healthcare Materials

Multivariate patterning of human pluripotent cells under perfusion reveals critical roles of induced paracrine factors in kidney organoid development

(2020) Nick R. Glass et al.   Science Advances

In Situ Expansion, Differentiation and Electromechanical Coupling of Human Cardiac Muscle in a 3D Bioprinted, Chambered Organoid

(2020) Molly E Kupfer et al.   CIRCULATION RESEARCH

Genetic Manipulation of Human Intestinal Enteroids Demonstrates the Necessity of a Functional Fucosyltransferase 2 Gene for Secretor-Dependent Human Norovirus Infection

(2020) Kei Haga et al.   mBio

Recapitulating Pancreatic Tumor Microenvironment through Synergistic Use of Patient Organoids and Organ‐on‐a‐Chip Vasculature

(2020) Benjamin Fook Lun Lai et al.   ADVANCED FUNCTIONAL MATERIALS

Recapitulating macro-scale tissue self-organization through organoid bioprinting

(2020) Jonathan A. Brassard et al.   NATURE MATERIALS

Advanced co-culture 3D breast cancer model for investigation of fibrosis induced by external stimuli: optimization study

(2020) Ilya Yakavets et al.   Scientific Reports

Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells

(2019) Amélie Montel-Hagen et al.   Cell Stem Cell

Flow-enhanced vascularization and maturation of kidney organoids in vitro

(2019) Kimberly A. Homan et al.   NATURE METHODS

Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells

(2019) Elena Garreta et al.   NATURE MATERIALS

Buoyancy-Driven Gradients for Biomaterial Fabrication and Tissue Engineering

(2019) Chunching Li et al.   ADVANCED MATERIALS

Growth factor delivery: Defining the next generation platforms for tissue engineering

(2019) Lilith M. Caballero Aguilar et al.   JOURNAL OF CONTROLLED RELEASE

Multivascular networks and functional intravascular topologies within biocompatible hydrogels

(2019) Bagrat Grigoryan et al.   SCIENCE

3D Bioprinting: from Benches to Translational Applications

(2019) Marcel Alexander Heinrich et al.   Small

Remote Control in Formation of 3D Multicellular Assemblies Using Magnetic Forces

(2019) Javad Jafari et al.   ACS Biomaterials-Science & Engineering

3D bioprinted mammary organoids and tumoroids in human mammary derived ECM hydrogels

(2019) Peter A. Mollica et al.   Acta Biomaterialia

Engineering Stem Cell Self-organization to Build Better Organoids

(2019) Jonathan A. Brassard et al.   Cell Stem Cell

Engineered signaling centers for the spatially controlled patterning of human pluripotent stem cells

(2019) Andrea Manfrin et al.   NATURE METHODS

Self-organization of stem cells into embryos: A window on early mammalian development

(2019) Marta N. Shahbazi et al.   SCIENCE

Optogenetics in the Era of Cerebral Organoids

(2019) Zahra Shiri et al.   TRENDS IN BIOTECHNOLOGY

A 3D bioprinter platform for mechanistic analysis of tumoroids and chimeric mammary organoids

(2019) John A. Reid et al.   Scientific Reports

Controlled modelling of human epiblast and amnion development using stem cells

(2019) Yi Zheng et al.   NATURE

Using Remote Fields for Complex Tissue Engineering

(2019) James P.K. Armstrong et al.   TRENDS IN BIOTECHNOLOGY

Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels

(2019) Mark A. Skylar-Scott et al.   Science Advances

Acoustic Holographic Cell Patterning in a Biocompatible Hydrogel

(2019) Zhichao Ma et al.   ADVANCED MATERIALS

Engineering cell–cell communication networks: programming multicellular behaviors

(2019) Satoshi Toda et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Synthetic developmental biology: build and control multicellular systems

(2019) Mo R Ebrahimkhani et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Engineering of human brain organoids with a functional vascular-like system

(2019) Bilal Cakir et al.   NATURE METHODS

Mouse embryo geometry drives formation of robust signaling gradients through receptor localization

(2019) Zhechun Zhang et al.   Nature Communications

Generation of complex human organoid models including vascular networks by incorporation of mesodermal progenitor cells

(2019) Philipp Wörsdörfer et al.   Scientific Reports

Remote Magnetic Nanoparticle Manipulation Enables the Dynamic Patterning of Cardiac Tissues

(2019) Limor Zwi‐Dantsis et al.   ADVANCED MATERIALS

A developmental landscape of 3D-cultured human pre-gastrulation embryos

(2019) Lifeng Xiang et al.   NATURE

Engineering innervated secretory epithelial organoids by magnetic three-dimensional bioprinting for stimulating epithelial growth in salivary glands

(2018) Christabella Adine et al.   BIOMATERIALS

A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity

(2018) Norman Sachs et al.   CELL

Synthetic embryology: controlling geometry to model early mammalian development

(2018) Jakob J Metzger et al.   CURRENT OPINION IN GENETICS & DEVELOPMENT

Blastocyst-like structures generated solely from stem cells

(2018) Nicolas C. Rivron et al.   NATURE

Human brain organoids on a chip reveal the physics of folding

(2018) Eyal Karzbrun et al.   Nature Physics

Programming self-organizing multicellular structures with synthetic cell-cell signaling

(2018) Satoshi Toda et al.   SCIENCE

Neanderthal brain organoids come to life

(2018) Jon Cohen   SCIENCE

Photoresponsive biomaterials for targeted drug delivery and 4D cell culture

(2018) Emily R. Ruskowitz et al.   Nature Reviews Materials

Mechanically induced development and maturation of human intestinal organoids in vivo

(2018) Holly M. Poling et al.   Nature Biomedical Engineering

Growth of Epithelial Organoids in a Defined Hydrogel

(2018) Nicolas Broguiere et al.   ADVANCED MATERIALS

Human iPS derived progenitors bioengineered into liver organoids using an inverted colloidal crystal poly (ethylene glycol) scaffold

(2018) Soon Seng Ng et al.   BIOMATERIALS

Progress and potential in organoid research

(2018) Giuliana Rossi et al.   NATURE REVIEWS GENETICS

Airflow-Assisted 3D Bioprinting of Human Heterogeneous Microspheroidal Organoids with Microfluidic Nozzle

(2018) Haiming Zhao et al.   Small

Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids

(2018) Magdalena Kasendra et al.   Scientific Reports

From morphogen to morphogenesis and back

(2017) Darren Gilmour et al.   NATURE

Guided self-organization and cortical plate formation in human brain organoids

(2017) Madeline A Lancaster et al.   NATURE BIOTECHNOLOGY

A three-dimensional model of human lung development and disease from pluripotent stem cells

(2017) Ya-Wen Chen et al.   NATURE CELL BIOLOGY

Organoid cystogenesis reveals a critical role of microenvironment in human polycystic kidney disease

(2017) Nelly M. Cruz et al.   NATURE MATERIALS

Fused cerebral organoids model interactions between brain regions

(2017) Joshua A Bagley et al.   NATURE METHODS

Assembly of embryonic and extraembryonic stem cells to mimic embryogenesis in vitro

(2017) Sarah Ellys Harrison et al.   SCIENCE

Disease Modeling in Stem Cell-Derived 3D Organoid Systems

(2017) Devanjali Dutta et al.   TRENDS IN MOLECULAR MEDICINE

Self-Organized Cerebral Organoids with Human-Specific Features Predict Effective Drugs to Combat Zika Virus Infection

(2017) Momoko Watanabe et al.   Cell Reports

Convergence of microengineering and cellular self-organization towards functional tissue manufacturing

(2017) Jérémie Laurent et al.   Nature Biomedical Engineering

Multi-tissue interactions in an integrated three-tissue organ-on-a-chip platform

(2017) Aleksander Skardal et al.   Scientific Reports

Neural patterning of human induced pluripotent stem cells in 3-D cultures for studying biomolecule-directed differential cellular responses

(2016) Yuanwei Yan et al.   Acta Biomaterialia

Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure

(2016) Xuyu Qian et al.   CELL

Development-on-chip:in vitroneural tube patterning with a microfluidic device

(2016) Christopher J. Demers et al.   DEVELOPMENT

Organoids: Modeling Development and the Stem Cell Niche in a Dish

(2016) Kai Kretzschmar et al.   DEVELOPMENTAL CELL

A morphospace for synthetic organs and organoids: the possible and the actual

(2016) Aina Ollé-Vila et al.   Integrative Biology

Visualization of a short-range Wnt gradient in the intestinal stem-cell niche

(2016) Henner F. Farin et al.   NATURE

Self-organization of the in vitro attached human embryo

(2016) Alessia Deglincerti et al.   NATURE

Designer matrices for intestinal stem cell and organoid culture

(2016) Nikolce Gjorevski et al.   NATURE

Organoids as an in vitro model of human development and disease

(2016) Aliya Fatehullah et al.   NATURE CELL BIOLOGY

Cell–cell communication enhances the capacity of cell ensembles to sense shallow gradients during morphogenesis

(2016) David Ellison et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Neural tube morphogenesis in synthetic 3D microenvironments

(2016) Adrian Ranga et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Replication of human noroviruses in stem cell-derived human enteroids

(2016) K. Ettayebi et al.   SCIENCE

Characterizing responses to CFTR-modulating drugs using rectal organoids derived from subjects with cystic fibrosis

(2016) Johanna F. Dekkers et al.   Science Translational Medicine

Front–Rear Polarization by Mechanical Cues: From Single Cells to Tissues

(2016) Benoit Ladoux et al.   TRENDS IN CELL BIOLOGY

Rapid Induction of Cerebral Organoids From Human Induced Pluripotent Stem Cells Using a Chemically Defined Hydrogel and Defined Cell Culture Medium

(2016) Beth A. Lindborg et al.   Stem Cells Translational Medicine

In Vitro Polarization of Colonoids to Create an Intestinal Stem Cell Compartment

(2016) Peter J. Attayek et al.   PLoS One

A Bio-Acoustic Levitational (BAL) Assembly Method for Engineering of Multilayered, 3D Brain-Like Constructs, Using Human Embryonic Stem Cell Derived Neuro-Progenitors

(2015) Charlène Bouyer et al.   ADVANCED MATERIALS

Prospective Derivation of a Living Organoid Biobank of Colorectal Cancer Patients

(2015) Marc van de Wetering et al.   CELL

Vascularized and Complex Organ Buds from Diverse Tissues via Mesenchymal Cell-Driven Condensation

(2015) Takanori Takebe et al.   Cell Stem Cell

Acquired EGFR C797S mutation mediates resistance to AZD9291 in non–small cell lung cancer harboring EGFR T790M

(2015) Kenneth S Thress et al.   NATURE MEDICINE

Simultaneous or Sequential Orthogonal Gradient Formation in a 3D Cell Culture Microfluidic Platform

(2015) Sebastien G. M. Uzel et al.   Small

Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue

(2015) Atsushi Kuwahara et al.   Nature Communications

Precision Assembly of Complex Cellular Microenvironments using Holographic Optical Tweezers

(2015) Glen R. Kirkham et al.   Scientific Reports

Modelling human development and disease in pluripotent stem-cell-derived gastric organoids

(2014) Kyle W. McCracken et al.   NATURE

Microfluidic organs-on-chips

(2014) Sangeeta N Bhatia et al.   NATURE BIOTECHNOLOGY

3D bioprinting of tissues and organs

(2014) Sean V Murphy et al.   NATURE BIOTECHNOLOGY

Generation of organized germ layers from a single mouse embryonic stem cell

(2014) Yeh-Chuin Poh et al.   Nature Communications

Transplantation of Embryonic and Induced Pluripotent Stem Cell-Derived 3D Retinal Sheets into Retinal Degenerative Mice

(2014) Juthaporn Assawachananont et al.   Stem Cell Reports

Transplantation of Expanded Fetal Intestinal Progenitors Contributes to Colon Regeneration after Injury

(2013) Robert P. Fordham et al.   Cell Stem Cell

Redefining the In Vivo Origin of Metanephric Nephron Progenitors Enables Generation of Complex Kidney Structures from Pluripotent Stem Cells

(2013) Atsuhiro Taguchi et al.   Cell Stem Cell

Cytosystems dynamics in self-organization of tissue architecture

(2013) Yoshiki Sasai   NATURE

In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration

(2013) Meritxell Huch et al.   NATURE

Cerebral organoids model human brain development and microcephaly

(2013) Madeline A. Lancaster et al.   NATURE

In situ cell manipulation through enzymatic hydrogel photopatterning

(2013) Katarzyna A. Mosiewicz et al.   NATURE MATERIALS

A functional CFTR assay using primary cystic fibrosis intestinal organoids

(2013) Johanna F Dekkers et al.   NATURE MEDICINE

Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications

(2013) T. Sato et al.   SCIENCE

How Cells Know Where They Are

(2013) A. D. Lander   SCIENCE

Self-Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs

(2012) Tokushige Nakano et al.   Cell Stem Cell

Diffusion- and convection-based activation of Wnt/β-catenin signaling in a gradient generating microfluidic chip

(2012) Chorong Kim et al.   LAB ON A CHIP

Effect of Substrate Stiffness on Early Mouse Embryo Development

(2012) Kevin S. Kolahi et al.   PLoS One

Self-organizing optic-cup morphogenesis in three-dimensional culture

(2011) Mototsugu Eiraku et al.   NATURE

Chemical Control of Stem Cell Fate and Developmental Potential

(2010) Costas A. Lyssiotis et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Biomimetic gradient hydrogels for tissue engineering

(2010) Shilpa Sant et al.   CANADIAN JOURNAL OF CHEMICAL ENGINEERING

Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro

(2010) Jason R. Spence et al.   NATURE

Three-dimensional tissue culture based on magnetic cell levitation

(2010) Glauco R. Souza et al.   Nature Nanotechnology

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

Programmed assembly of 3-dimensional microtissues with defined cellular connectivity

(2009) Z. J. Gartner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals

(2008) Mototsugu Eiraku et al.   Cell Stem Cell

Tensile forces govern germ-layer organization in zebrafish

(2008) M. Krieg et al.   NATURE CELL BIOLOGY

Mechanotransduction gone awry

(2008) Diana E. Jaalouk et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY