Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells

SUR1-mutant iPS cell-derived islets recapitulate the pathophysiology of congenital hyperinsulinism

(2021) Väinö Lithovius et al.   DIABETOLOGIA

SIX2 and SIX3 coordinately regulate functional maturity and fate of human pancreatic β cells

(2021) Romina J. Bevacqua et al.   GENES & DEVELOPMENT

Mechanisms controlling pancreatic islet cell function in insulin secretion

(2021) Jonathan E. Campbell et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

CellProfiler 4: improvements in speed, utility and usability

(2021) David R. Stirling et al.   BMC BIOINFORMATICS

A Nutrient-Sensing Transition at Birth Triggers Glucose-Responsive Insulin Secretion

(2020) Aharon Helman et al.   Cell Metabolism

Generation of pancreatic β cells from CD177+ anterior definitive endoderm

(2020) Pallavi U. Mahaddalkar et al.   NATURE BIOTECHNOLOGY

Glucose Response by Stem Cell-Derived β Cells In Vitro Is Inhibited by a Bottleneck in Glycolysis

(2020) Jeffrey C. Davis et al.   Cell Reports

Generalizing RNA velocity to transient cell states through dynamical modeling

(2020) Volker Bergen et al.   NATURE BIOTECHNOLOGY

Alpha cell regulation of beta cell function

(2020) Tilo Moede et al.   DIABETOLOGIA

Immune-evasive human islet-like organoids ameliorate diabetes

(2020) Eiji Yoshihara et al.   NATURE

Single-Cell Transcriptome Profiling Reveals β Cell Maturation in Stem Cell-Derived Islets after Transplantation

(2020) Punn Augsornworawat et al.   Cell Reports

Reductive TCA cycle metabolism fuels glutamine- and glucose-stimulated insulin secretion

(2020) Guo-Fang Zhang et al.   Cell Metabolism

SoupX removes ambient RNA contamination from droplet-based single-cell RNA sequencing data

(2020) Matthew D Young et al.   GigaScience

SCRT1 is a novel beta cell transcription factor with insulin regulatory properties

(2020) S. Chriett et al.   MOLECULAR AND CELLULAR ENDOCRINOLOGY

Acquisition of Dynamic Function in Human Stem Cell-Derived β Cells

(2019) Leonardo Velazco-Cruz et al.   Stem Cell Reports

Recapitulating endocrine cell clustering in culture promotes maturation of human stem-cell-derived β cells

(2019) Gopika G. Nair et al.   NATURE CELL BIOLOGY

Charting cellular identity during human in vitro β-cell differentiation

(2019) Adrian Veres et al.   NATURE

Fusion pore regulation by cAMP/Epac2 controls cargo release during insulin exocytosis

(2019) Alenka Guček et al.   eLife

mTORC1-to-AMPK switching underlies β cell metabolic plasticity during maturation and diabetes

(2019) Rami Jaafar et al.   JOURNAL OF CLINICAL INVESTIGATION

Fast, sensitive and accurate integration of single-cell data with Harmony

(2019) Ilya Korsunsky et al.   NATURE METHODS

Circadian Entrainment Triggers Maturation of Human In Vitro Islets

(2019) Juan R. Alvarez-Dominguez et al.   Cell Stem Cell

Recovering Gene Interactions from Single-Cell Data Using Data Diffusion

(2018) David van Dijk et al.   CELL

Glucose-Dependent Granule Docking Limits Insulin Secretion and Is Decreased in Human Type 2 Diabetes

(2018) Nikhil R. Gandasi et al.   Cell Metabolism

Paracrine Interactions within the Pancreatic Islet Determine the Glycemic Set Point

(2018) Rayner Rodriguez-Diaz et al.   Cell Metabolism

Pseudotime Ordering of Single Human β-Cells Reveals States of Insulin Production and Unfolded Protein Response

(2018) Yurong Xin et al.   DIABETES

Integrating single-cell transcriptomic data across different conditions, technologies, and species

(2018) Andrew Butler et al.   NATURE BIOTECHNOLOGY

Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men

(2018) Patrik Rorsman et al.   PHYSIOLOGICAL REVIEWS

Replication confers β cell immaturity

(2018) Sapna Puri et al.   Nature Communications

Trefoil factor 3 in perinatal pancreas is increased by gestational low protein diet and associated with accelerated β-cell maturation

(2018) Louise Winkel et al.   Islets

Understanding human fetal pancreas development using subpopulation sorting, RNA sequencing and single-cell profiling

(2018) Cyrille Ramond et al.   DEVELOPMENT

RNA velocity of single cells

(2018) Gioele La Manno et al.   NATURE

Comparative Analysis of Droplet-Based Ultra-High-Throughput Single-Cell RNA-Seq Systems

(2018) Xiannian Zhang et al.   MOLECULAR CELL

Concise Review: Human Pluripotent Stem Cells for the Modeling of Pancreatic β-Cell Pathology

(2018) Diego Balboa et al.   STEM CELLS

Integrated In Vivo Quantitative Proteomics and Nutrient Tracing Reveals Age-Related Metabolic Rewiring of Pancreatic β Cell Function

(2018) Matthew Wortham et al.   Cell Reports

Insulin mutations impair beta-cell development in a patient-derived iPSC model of neonatal diabetes

(2018) Diego Balboa et al.   eLife

Single-Cell Transcriptome Profiling of Mouse and hESC-Derived Pancreatic Progenitors

(2018) Nicole A.J. Krentz et al.   Stem Cell Reports

Rho-Associated Kinases and Non-muscle Myosin IIs Inhibit the Differentiation of Human iPSCs to Pancreatic Endoderm

(2017) Taro Toyoda et al.   Stem Cell Reports

Reduced Insulin Production Relieves Endoplasmic Reticulum Stress and Induces β Cell Proliferation

(2016) Marta Szabat et al.   Cell Metabolism

Age-Dependent Pancreatic Gene Regulation Reveals Mechanisms Governing Human β Cell Function

(2016) H. Efsun Arda et al.   Cell Metabolism

Single-Cell Transcriptome Profiling of Human Pancreatic Islets in Health and Type 2 Diabetes

(2016) Åsa Segerstolpe et al.   Cell Metabolism

Research-Focused Isolation of Human Islets From Donors With and Without Diabetes at the Alberta Diabetes Institute IsletCore

(2016) James Lyon et al.   ENDOCRINOLOGY

Enrichr: a comprehensive gene set enrichment analysis web server 2016 update

(2016) Maxim V. Kuleshov et al.   NUCLEIC ACIDS RESEARCH

The Pancreatic β-Cell: A Bioenergetic Perspective

(2016) David G. Nicholls   PHYSIOLOGICAL REVIEWS

A Single-Cell Transcriptomic Map of the Human and Mouse Pancreas Reveals Inter- and Intra-cell Population Structure

(2016) Maayan Baron et al.   Cell Systems

Dynamics of glucose-induced insulin secretion in normal human islets

(2015) Jean-Claude Henquin et al.   AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM

Isocitrate-to-SENP1 signaling amplifies insulin secretion and rescues dysfunctional β cells

(2015) Mourad Ferdaoussi et al.   JOURNAL OF CLINICAL INVESTIGATION

Generation of iPSC line HEL24.3 from human neonatal foreskin fibroblasts

(2015) Ras Trokovic et al.   Stem Cell Research

Aurora Kinase A is critical for the Nkx6.1 mediated β-cell proliferation pathway

(2015) Amanda Hobson et al.   Islets

Efficient Generation of NKX6-1+ Pancreatic Progenitors from Multiple Human Pluripotent Stem Cell Lines

(2015) M. Cristina Nostro et al.   Stem Cell Reports

A human beta cell line with drug inducible excision of immortalizing transgenes

(2015) Marion Benazra et al.   Molecular Metabolism

Characterization of Stimulus-Secretion Coupling in the Human Pancreatic EndoC-βH1 Beta Cell Line

(2015) Lotta E. Andersson et al.   PLoS One

Fourth-Generation Epac-Based FRET Sensors for cAMP Feature Exceptional Brightness, Photostability and Dynamic Range: Characterization of Dedicated Sensors for FLIM, for Ratiometry and with High Affinity

(2015) Jeffrey Klarenbeek et al.   PLoS One

Generation of Functional Human Pancreatic β Cells In Vitro

(2014) Felicia W. Pagliuca et al.   CELL

The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells

(2014) Cole Trapnell et al.   NATURE BIOTECHNOLOGY

Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells

(2014) Alireza Rezania et al.   NATURE BIOTECHNOLOGY

Differentiated human stem cells resemble fetal, not adult, β cells

(2014) Sinisa Hrvatin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrastructural and immunohistochemical analysis of the 8-20 week human fetal pancreas

(2014) Matthew Riopel et al.   Islets

Reduction of Reactive Oxygen Species Ameliorates Metabolism-Secretion Coupling in Islets of Diabetic GK Rats by Suppressing Lactate Overproduction

(2013) M. Sasaki et al.   DIABETES

Enrichment of human embryonic stem cell-derived NKX6.1-expressing pancreatic progenitor cells accelerates the maturation of insulin-secreting cells in vivo

(2013) Alireza Rezania et al.   STEM CELLS

Regulation of Insulin Secretion in Human Pancreatic Islets

(2012) Patrik Rorsman et al.   Annual Review of Physiology

Maturation of Human Embryonic Stem Cell-Derived Pancreatic Progenitors Into Functional Islets Capable of Treating Pre-existing Diabetes in Mice

(2012) A. Rezania et al.   DIABETES

The Zinc Finger Protein ZBTB20 Regulates Transcription of Fructose-1,6-Bisphosphatase 1 and β Cell Function in Mice

(2012) Ye Zhang et al.   GASTROENTEROLOGY

Formation of a Human β-Cell Population within Pancreatic Islets Is Set Early in Life

(2012) Brigid E. Gregg et al.   JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM

A Novel High-Throughput Assay for Islet Respiration Reveals Uncoupling of Rodent and Human Islets

(2012) Jakob D. Wikstrom et al.   PLoS One

The functional and molecular characterisation of human embryonic stem cell-derived insulin-positive cells compared with adult pancreatic beta cells

(2011) C. L. Basford et al.   DIABETOLOGIA

Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells

(2011) Olivia G Kelly et al.   NATURE BIOTECHNOLOGY

Production of Functional Glucagon-Secreting  -Cells From Human Embryonic Stem Cells

(2010) A. Rezania et al.   DIABETES

The Mitochondrial 2-Oxoglutarate Carrier Is Part of a Metabolic Pathway That Mediates Glucose- and Glutamine-stimulated Insulin Secretion

(2010) Matthew L. Odegaard et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Regulation of insulin secretion: a matter of phase control and amplitude modulation

(2009) J. C. Henquin   DIABETOLOGIA

Endocrine Cell Clustering During Human Pancreas Development

(2009) Jongmin Jeon et al.   JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY

Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo

(2008) Evert Kroon et al.   NATURE BIOTECHNOLOGY