(R)evolution-on-a-chip

‘NAD‐display’: Ultrahigh‐Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry

(2021) Laurens Lindenburg et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput

(2021) Josephin M. Holstein et al.   ACS Synthetic Biology

Directed evolution of adenine base editors with increased activity and therapeutic application

(2020) Nicole M. Gaudelli et al.   NATURE BIOTECHNOLOGY

Coupling droplet microfluidics with mass spectrometry for ultra-high-throughput analysis of complex mixtures up to and above 30Hz.

(2020) Emily E. Kempa et al.   ANALYTICAL CHEMISTRY

Microfluidic production and characterization of biofunctionalized giant unilamellar vesicles for targeted intracellular cargo delivery

(2020) Oskar Staufer et al.   BIOMATERIALS

UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution

(2020) Paul Jannis Zurek et al.   Nature Communications

Crystal Structure and Directed Evolution of Specificity of NlaIV Restriction Endonuclease

(2019) Honorata Czapinska et al.   JOURNAL OF MOLECULAR BIOLOGY

Fluorescence-Activated Droplet Sorting for Single-Cell Directed Evolution

(2019) Derek Vallejo et al.   ACS Synthetic Biology

Scalable Production of Monodisperse Functional Microspheres by Multilayer Parallelization of High Aspect Ratio Microfluidic Channels

(2019) Casper Ho Yin Chung et al.   Micromachines

Ultrahigh-throughput screening enables efficient single-round oxidase remodelling

(2019) Aaron Debon et al.   Nature Catalysis

Cell-free gene expression: an expanded repertoire of applications

(2019) Adam D. Silverman et al.   NATURE REVIEWS GENETICS

High-Throughput, Lysis-Free Screening for Sulfatase Activity Using Escherichia coli Autodisplay in Microdroplets

(2019) Bert van Loo et al.   ACS Synthetic Biology

Droplet Microfluidics-Enabled High-Throughput Screening for Protein Engineering

(2019) Lindong Weng et al.   Micromachines

Mass Activated Droplet Sorting (MADS) Enables High-Throughput Screening of Enzymatic Reactions at Nanoliter Scale

(2019) Daniel A. Holland-Moritz et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Tailoring the appearance: what will synthetic cells look like?

(2018) Willem Kasper Spoelstra et al.   CURRENT OPINION IN BIOTECHNOLOGY

A highly specific SpCas9 variant is identified by in vivo screening in yeast

(2018) Antonio Casini et al.   NATURE BIOTECHNOLOGY

On-chip microfluidic production of cell-sized liposomes

(2018) Siddharth Deshpande et al.   Nature Protocols

Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform

(2018) Fuqiang Ma et al.   Nature Communications

Cell-free supplement mixtures: Elucidating the history and biochemical utility of additives used to support in vitro protein synthesis in E. coli extract

(2018) By Jared L. Dopp et al.   BIOTECHNOLOGY ADVANCES

Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica

(2017) Thomas Beneyton et al.   Microbial Cell Factories

Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics

(2017) Marian Weiss et al.   NATURE MATERIALS

Ultrahigh-Throughput Improvement and Discovery of Enzymes Using Droplet-Based Microfluidic Screening

(2017) Alexis Autour et al.   Micromachines

Magnetophoretic sorting of microdroplets with different microalgal cell densities for rapid isolation of fast growing strains

(2017) Young Joon Sung et al.   Scientific Reports

Monodisperse Uni- and Multicompartment Liposomes

(2016) Nan-Nan Deng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Electrocoalescence of paired droplets encapsulated in double-emulsion drops

(2016) Yankan Jia et al.   LAB ON A CHIP

Emergence of a catalytic tetrad during evolution of a highly active artificial aldolase

(2016) Richard Obexer et al.   Nature Chemistry

iSpinach: a fluorogenic RNA aptamer optimized forin vitroapplications

(2016) Alexis Autour et al.   NUCLEIC ACIDS RESEARCH

Evolution of a mass spectrometry-grade protease with PTM-directed specificity

(2016) Duc T. Tran et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)

(2016) Fabrice Gielen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Octanol-assisted liposome assembly on chip

(2016) Siddharth Deshpande et al.   Nature Communications

In vivo continuous evolution of genes and pathways in yeast

(2016) Nathan Crook et al.   Nature Communications

A general strategy for expanding polymerase function by droplet microfluidics

(2016) Andrew C. Larsen et al.   Nature Communications

Yarrowia lipolytica: recent achievements in heterologous protein expression and pathway engineering

(2015) Catherine Madzak   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Activity-Fed Translation (AFT) Assay: A New High-Throughput Screening Strategy for Enzymes in Droplets

(2015) Gabrielle Woronoff et al.   CHEMBIOCHEM

Liposome-Based in Vitro Evolution of Aminoacyl-tRNA Synthetase for Enhanced Pyrrolysine Derivative Incorporation

(2015) Atsuko Uyeda et al.   CHEMBIOCHEM

Enzyme engineering in biomimetic compartments

(2015) Pierre-Yves Colin et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Accurate microfluidic sorting of droplets at 30 kHz

(2015) Adam Sciambi et al.   LAB ON A CHIP

Advances in fabricating double-emulsion droplets and their biomedical applications

(2015) DaoTong Chong et al.   Microfluidics and Nanofluidics

Methods for the directed evolution of proteins

(2015) Michael S. Packer et al.   NATURE REVIEWS GENETICS

Using droplet-based microfluidics to improve the catalytic properties of RNA under multiple-turnover conditions

(2015) Michael Ryckelynck et al.   RNA

Bioinspired genotype-phenotype linkages: mimicking cellular compartmentalization for the engineering of functional proteins

(2015) L. D. van Vliet et al.   Interface Focus

CotA laccase: high-throughput manipulation and analysis of recombinant enzyme libraries expressed in E. coli using droplet-based microfluidics

(2014) Thomas Beneyton et al.   ANALYST

Droplet Electrospray Ionization Mass Spectrometry for High Throughput Screening for Enzyme Inhibitors

(2014) Shuwen Sun et al.   ANALYTICAL CHEMISTRY

One in a Million: Flow Cytometric Sorting of Single Cell-Lysate Assays in Monodisperse Picolitre Double Emulsion Droplets for Directed Evolution

(2014) Anastasia Zinchenko et al.   ANALYTICAL CHEMISTRY

Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption

(2014) Benjamin L Wang et al.   NATURE BIOTECHNOLOGY

Evolution of enzyme catalysts caged in biomimetic gel-shell beads

(2014) Martin Fischlechner et al.   Nature Chemistry

Redesign of extensive protein-DNA interfaces of meganucleases using iterative cycles of in vitro compartmentalization

(2014) R. Takeuchi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrarapid Generation of Femtoliter Microfluidic Droplets for Single-Molecule-Counting Immunoassays

(2013) Jung-uk Shim et al.   ACS Nano

High-throughput screening for industrial enzyme production hosts by droplet microfluidics

(2013) Staffan L. Sjostrom et al.   LAB ON A CHIP

Directed evolution of genetic parts and circuits by compartmentalized partnered replication

(2013) Jared W Ellefson et al.   NATURE BIOTECHNOLOGY

In vitro evolution of  -hemolysin using a liposome display

(2013) S. Fujii et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Electrode-free picoinjection of microfluidic drops

(2012) Brian O'Donovan et al.   LAB ON A CHIP

A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution

(2012) Ali Fallah-Araghi et al.   LAB ON A CHIP

Faster multiple emulsification with drop splitting

(2011) Adam R. Abate et al.   LAB ON A CHIP

mRNA display for the selection and evolution of enzymes from in vitro-translated protein libraries

(2011) Burckhard Seelig   Nature Protocols

RNA Mimics of Green Fluorescent Protein

(2011) J. S. Paige et al.   SCIENCE

Giant Vesicles: Preparations and Applications

(2010) Peter Walde et al.   CHEMBIOCHEM

Development of an In Vitro Compartmentalization Screen for High-Throughput Directed Evolution of [FeFe] Hydrogenases

(2010) James A. Stapleton et al.   PLoS One

High-throughput injection with microfluidics using picoinjectors

(2010) A. R. Abate et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

(2010) J. J. Agresti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Droplet-Based Microfluidic Systems for High-Throughput Single DNA Molecule Isothermal Amplification and Analysis

(2009) Linas Mazutis et al.   ANALYTICAL CHEMISTRY

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

(2009) Jean-Christophe Baret et al.   LAB ON A CHIP

An Integrated Device for Monitoring Time-Dependent in vitro Expression From Single Genes in Picolitre Droplets

(2008) Fabienne Courtois et al.   CHEMBIOCHEM