Droplet Microfluidics-Enabled High-Throughput Screening for Protein Engineering

Droplet Microfluidics: Coding of Experimental Conditions in Microfluidic Droplet Assays Using Colored Beads and Machine Learning Supported Image Analysis (Small 4/2019)

(2019) Carl-Magnus Svensson et al.   Small

Engineered microscale hydrogels for drug delivery, cell therapy, and sequencing

(2019) Marissa E. Wechsler et al.   BIOMEDICAL MICRODEVICES

Fluorescence-Activated Droplet Sorting for Single-Cell Directed Evolution

(2019) Derek Vallejo et al.   ACS Synthetic Biology

Machine-learning-guided directed evolution for protein engineering

(2019) Kevin K. Yang et al.   NATURE METHODS

Oscillatory inertial focusing in infinite microchannels

(2018) Baris R. Mutlu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform

(2018) Fuqiang Ma et al.   Nature Communications

An Oil-Free Picodrop Bioassay Platform for Synthetic Biology

(2018) Christian A. Siltanen et al.   Scientific Reports

Intelligent Image-Activated Cell Sorting

(2018) Nao Nitta et al.   CELL

Current trends in protein engineering: updates and progress

(2018) Rajeshwari Sinha et al.   CURRENT PROTEIN & PEPTIDE SCIENCE

Deep Learning with Microfluidics for Biotechnology

(2018) Jason Riordon et al.   TRENDS IN BIOTECHNOLOGY

High-throughput multiplexed fluorescence-activated droplet sorting

(2018) Ouriel Caen et al.   Microsystems & Nanoengineering

Microfluidics for cell factory and bioprocess development

(2018) Sara M Bjork et al.   CURRENT OPINION IN BIOTECHNOLOGY

Microfluidic train station: highly robust and multiplexable sorting of droplets on electric rails

(2017) Daniel Frenzel et al.   LAB ON A CHIP

Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells

(2017) Russell H. Cole et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity

(2017) Stanislav S. Terekhov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bacterial Microcolonies in Gel Beads for High-Throughput Screening of Libraries in Synthetic Biology

(2017) José M. Duarte et al.   ACS Synthetic Biology

Selection platforms for directed evolution in synthetic biology

(2016) P. A. G. Tizei et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Engineering Cellular Metabolism

(2016) Jens Nielsen et al.   CELL

Directed evolution and synthetic biology applications to microbial systems

(2016) Marcelo C Bassalo et al.   CURRENT OPINION IN BIOTECHNOLOGY

High-throughput screening of antibiotic-resistant bacteria in picodroplets

(2016) X. Liu et al.   LAB ON A CHIP

Fluorinated Pickering Emulsions with Nonadsorbing Interfaces for Droplet-based Enzymatic Assays

(2015) Ming Pan et al.   ANALYTICAL CHEMISTRY

Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells

(2015) Allon M. Klein et al.   CELL

Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets

(2015) Evan Z. Macosko et al.   CELL

Stabilisers for water-in-fluorinated-oil dispersions: Key properties for microfluidic applications

(2015) Philipp Gruner et al.   CURRENT OPINION IN COLLOID & INTERFACE SCIENCE

Chemically induced coalescence in droplet-based microfluidics

(2015) Ilke Akartuna et al.   LAB ON A CHIP

Protein engineering in the 21stcentury

(2015) Roberto A. Chica   PROTEIN SCIENCE

Fluorinated Pickering Emulsions Impede Interfacial Transport and Form Rigid Interface for the Growth of Anchorage-Dependent Cells

(2014) Ming Pan et al.   ACS Applied Materials & Interfaces

One in a Million: Flow Cytometric Sorting of Single Cell-Lysate Assays in Monodisperse Picolitre Double Emulsion Droplets for Directed Evolution

(2014) Anastasia Zinchenko et al.   ANALYTICAL CHEMISTRY

Bioinspired Insights into Silicic Acid Stabilization Mechanisms: The Dominant Role of Polyethylene Glycol-Induced Hydrogen Bonding

(2014) Melina Preari et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Sugar Additives Improve Signal Fidelity for Implementing Two-Phase Resorufin-Based Enzyme Immunoassays

(2014) Patrick A. Sandoz et al.   LANGMUIR

Controlled multistep synthesis in a three-phase droplet reactor

(2014) Adrian M. Nightingale et al.   Nature Communications

Microfluidics-Assisted Fabrication of Gelatin-Silica Core–Shell Microgels for Injectable Tissue Constructs

(2013) Chaenyung Cha et al.   BIOMACROMOLECULES

Adding reagent to droplets with controlled rupture of encapsulated double emulsions

(2013) Adam Sciambi et al.   Biomicrofluidics

High-throughput screening for industrial enzyme production hosts by droplet microfluidics

(2013) Staffan L. Sjostrom et al.   LAB ON A CHIP

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

High-throughput co-encapsulation of self-ordered cell trains: cell pair interactions in microdroplets

(2013) Todd P. Lagus et al.   RSC Advances

Characterization of sensitivity and specificity in leaky droplet-based assays

(2012) Yunhan Chen et al.   LAB ON A CHIP

A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution

(2012) Ali Fallah-Araghi et al.   LAB ON A CHIP

Functional single-cell hybridoma screening using droplet-based microfluidics

(2012) B. E. Debs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Dynamics of molecular transport by surfactants in emulsions

(2012) Yousr Skhiri et al.   Soft Matter

New Generation of Amino Coumarin Methyl Sulfonate-Based Fluorogenic Substrates for Amidase Assays in Droplet-Based Microfluidic Applications

(2011) Gabrielle Woronoff et al.   ANALYTICAL CHEMISTRY

Analysis of gene expression at the single-cell level using microdroplet-based microfluidic technology

(2011) Pascaline Mary et al.   Biomicrofluidics

Droplet microfluidics—a tool for protein engineering and analysis

(2011) Haakan N. Joensson et al.   LAB ON A CHIP

Droplet size based separation by deterministic lateral displacement—separating droplets by cell-induced shrinking

(2011) Haakan N. Joensson et al.   LAB ON A CHIP

Surfactants in droplet-based microfluidics

(2011) Jean-Christophe Baret   LAB ON A CHIP

Encapsulating Bacteria in Agarose Microparticles Using Microfluidics for High-Throughput Cell Analysis and Isolation

(2010) Ye-Jin Eun et al.   ACS Chemical Biology

Microfluidic Compartmentalized Directed Evolution

(2010) Brian M. Paegel et al.   CHEMISTRY & BIOLOGY

Electrocoalescence Mechanisms of Microdroplets Using Localized Electric Fields in Microfluidic Channels

(2010) Michele Zagnoni et al.   LANGMUIR

High-throughput injection with microfluidics using picoinjectors

(2010) A. R. Abate et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

(2010) J. J. Agresti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Solid Nanoparticles that Catalyze Biofuel Upgrade Reactions at the Water/Oil Interface

(2010) S. Crossley et al.   SCIENCE

Controlling the Retention of Small Molecules in Emulsion Microdroplets for Use in Cell-Based Assays

(2009) Fabienne Courtois et al.   ANALYTICAL CHEMISTRY

Directed evolution: new parts and optimized function

(2009) Michael J Dougherty et al.   CURRENT OPINION IN BIOTECHNOLOGY

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

(2009) Jean-Christophe Baret et al.   LAB ON A CHIP

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Droplet microfluidic technology for single-cell high-throughput screening

(2009) E. Brouzes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

From Microdroplets to Microfluidics: Selective Emulsion Separation in Microfluidic Devices

(2008) Luis M. Fidalgo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Controlled encapsulation of single-cells into monodisperse picolitre drops

(2008) Jon F. Edd et al.   LAB ON A CHIP

Pillar-induced droplet merging in microfluidic circuits

(2008) Xize Niu et al.   LAB ON A CHIP

Continuous generation of hydrogel beads and encapsulation of biological materials using a microfluidic droplet-merging channel

(2008) Eujin Um et al.   Microfluidics and Nanofluidics