Journal
SCIENCE
Volume 333, Issue 6042, Pages 642-646Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1207339
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Funding
- McKnight Neuroscience Technology Innovation Award
- Weill Cornell Medical College
- National Institute of Neurological Disorders and Stroke [NS064516, T32CA062948]
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Green fluorescent protein (GFP) and its derivatives have transformed the use and analysis of proteins for diverse applications. Like proteins, RNA has complex roles in cellular function and is increasingly used for various applications, but a comparable approach for fluorescently tagging RNA is lacking. Here, we describe the generation of RNA aptamers that bind fluorophores resembling the fluorophore in GFP. These RNA-fluorophore complexes create a palette that spans the visible spectrum. An RNA-fluorophore complex, termed Spinach, resembles enhanced GFP and emits a green fluorescence comparable in brightness with fluorescent proteins. Spinach is markedly resistant to photobleaching, and Spinach fusion RNAs can be imaged in living cells. These RNA mimics of GFP provide an approach for genetic encoding of fluorescent RNAs.
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