Ultrahigh-throughput screening enables efficient single-round oxidase remodelling

Kinetic Resolution of sec -Thiols by Enantioselective Oxidation with Rationally Engineered 5-(Hydroxymethyl)furfural Oxidase

(2018) Mathias Pickl et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Speeding up enzyme discovery and engineering with ultrahigh-throughput methods

(2018) Hans Adrian Bunzel et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Getting Momentum: From Biocatalysis to Advanced Synthetic Biology

(2018) Christoffel P.S. Badenhorst et al.   TRENDS IN BIOCHEMICAL SCIENCES

Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform

(2018) Fuqiang Ma et al.   Nature Communications

Biocatalytic Route to Chiral 2-Substituted-1,2,3,4-Tetrahydroquinolines Using Cyclohexylamine Oxidase Muteins

(2018) Peiyuan Yao et al.   ACS Catalysis

Ultra-high throughput functional enrichment of large monoamine oxidase (MAO-N) libraries by fluorescence activated cell sorting

(2018) Joanna C. Sadler et al.   ANALYST

Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica

(2017) Thomas Beneyton et al.   Microbial Cell Factories

Enantioselective, intermolecular benzylic C–H amination catalysed by an engineered iron-haem enzyme

(2017) Christopher K. Prier et al.   Nature Chemistry

A reductive aminase from Aspergillus oryzae

(2017) Godwin A. Aleku et al.   Nature Chemistry

Simultaneous engineering of an enzyme's entrance tunnel and active site: the case of monoamine oxidase MAO-N

(2017) Guangyue Li et al.   Chemical Science

Biocatalysis in the Pharmaceutical Industry: The Need for Speed

(2017) Matthew D. Truppo   ACS Medicinal Chemistry Letters

Emergence of a catalytic tetrad during evolution of a highly active artificial aldolase

(2016) Richard Obexer et al.   Nature Chemistry

Identification of (S)-selective transaminases for the asymmetric synthesis of bulky chiral amines

(2016) Ioannis V. Pavlidis et al.   Nature Chemistry

Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)

(2016) Fabrice Gielen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient laboratory evolution of computationally designed enzymes with low starting activities using fluorescence-activated droplet sorting

(2016) Richard Obexer et al.   PROTEIN ENGINEERING DESIGN & SELECTION

New recombinant cyclohexylamine oxidase variants for deracemization of secondary amines by orthogonally assaying designed mutants with structurally diverse substrates

(2016) Guangyue Li et al.   Scientific Reports

High-throughput screening of filamentous fungi using nanoliter-range droplet-based microfluidics

(2016) Thomas Beneyton et al.   Scientific Reports

Quantitative Packaging of Active Enzymes into a Protein Cage

(2015) Yusuke Azuma et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Methods for the directed evolution of proteins

(2015) Michael S. Packer et al.   NATURE REVIEWS GENETICS

Conversion of alcohols to enantiopure amines through dual-enzyme hydrogen-borrowing cascades

(2015) F. G. Mutti et al.   SCIENCE

Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics

(2015) Pierre-Yves Colin et al.   Nature Communications

Functional Mapping of Protein-Protein Interactions in an Enzyme Complex by Directed Evolution

(2015) Kathrin Roderer et al.   PLoS One

Droplet-Based Microfluidics Platform for Ultra-High-Throughput Bioprospecting of Cellulolytic Microorganisms

(2014) Majdi Najah et al.   CHEMISTRY & BIOLOGY

Engineering chiral porous metal-organic frameworks for enantioselective adsorption and separation

(2014) Yongwu Peng et al.   Nature Communications

Substrate profiling of cyclohexylamine oxidase and its mutants reveals new biocatalytic potential in deracemization of racemic amines

(2013) Guangyue Li et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering an Enantioselective Amine Oxidase for the Synthesis of Pharmaceutical Building Blocks and Alkaloid Natural Products

(2013) Diego Ghislieri et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Monoamine Oxidase (MAO-N) Catalyzed Deracemization of Tetrahydro-β-carbolines: Substrate Dependent Switch in Enantioselectivity

(2013) Diego Ghislieri et al.   ACS Catalysis

Picoliter Cell Lysate Assays in Microfluidic Droplet Compartments for Directed Enzyme Evolution

(2012) Balint Kintses et al.   CHEMISTRY & BIOLOGY

Efficient, Chemoenzymatic Process for Manufacture of the Boceprevir Bicyclic [3.1.0]Proline Intermediate Based on Amine Oxidase-Catalyzed Desymmetrization

(2012) Tao Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

(2010) J. J. Agresti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

(2010) C. K. Savile et al.   SCIENCE

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

(2009) Jean-Christophe Baret et al.   LAB ON A CHIP

Impact of inlet channel geometry on microfluidic drop formation

(2009) A. R. Abate et al.   PHYSICAL REVIEW E

Directed Evolution of Galactose Oxidase: Generation of Enantioselective Secondary Alcohol Oxidases

(2008) Franck Escalettes et al.   CHEMBIOCHEM

Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms

(2008) Jenifer Clausell-Tormos et al.   CHEMISTRY & BIOLOGY

Reliable microfluidic on-chip incubation of droplets in delay-lines

(2008) Lucas Frenz et al.   LAB ON A CHIP