Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform

Ultrahigh-Throughput Improvement and Discovery of Enzymes Using Droplet-Based Microfluidic Screening

(2017) Alexis Autour et al.   Micromachines

In Vitro and In Vivo Enzyme Activity Screening via RNA-Based Fluorescent Biosensors for S-Adenosyl-l-homocysteine (SAH)

(2016) Yichi Su et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Efficient laboratory evolution of computationally designed enzymes with low starting activities using fluorescence-activated droplet sorting

(2016) Richard Obexer et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A general strategy for expanding polymerase function by droplet microfluidics

(2016) Andrew C. Larsen et al.   Nature Communications

Transcription factor-based biosensors in biotechnology: current state and future prospects

(2015) Regina Mahr et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Droplet-based microfluidics for high-throughput screening of a metagenomic library for isolation of microbial enzymes

(2015) Masahito Hosokawa et al.   BIOSENSORS & BIOELECTRONICS

Improving and repurposing biocatalysts via directed evolution

(2015) Carl A Denard et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast

(2015) Mingtao Huang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Using droplet-based microfluidics to improve the catalytic properties of RNA under multiple-turnover conditions

(2015) Michael Ryckelynck et al.   RNA

Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics

(2015) Pierre-Yves Colin et al.   Nature Communications

One in a Million: Flow Cytometric Sorting of Single Cell-Lysate Assays in Monodisperse Picolitre Double Emulsion Droplets for Directed Evolution

(2014) Anastasia Zinchenko et al.   ANALYTICAL CHEMISTRY

Droplet-Based Microfluidics Platform for Ultra-High-Throughput Bioprospecting of Cellulolytic Microorganisms

(2014) Majdi Najah et al.   CHEMISTRY & BIOLOGY

High Throughput Screening and Selection Methods for Directed Enzyme Evolution

(2014) Han Xiao et al.   INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

Directed evolution of stereoselective enzymes based on genetic selection as opposed to screening systems

(2014) Carlos G. Acevedo-Rocha et al.   JOURNAL OF BIOTECHNOLOGY

Evolution of enzyme catalysts caged in biomimetic gel-shell beads

(2014) Martin Fischlechner et al.   Nature Chemistry

An Improved Single Cell Ultrahigh Throughput Screening Method Based on In Vitro Compartmentalization

(2014) Fuqiang Ma et al.   PLoS One

Development of a highly sensitive, high-throughput assay for glycosyltransferases using enzyme-coupled fluorescence detection

(2013) Kazuo Kumagai et al.   ANALYTICAL BIOCHEMISTRY

Double strand DNA-templated copper nanoparticle as a novel fluorescence indicator for label-free detection of polynucleotide kinase activity

(2013) Liangliang Zhang et al.   BIOSENSORS & BIOELECTRONICS

Laboratory Evolution of Enantiocomplementary Candida antarctica Lipase B Mutants with Broad Substrate Scope

(2013) Qi Wu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

High-throughput screening for industrial enzyme production hosts by droplet microfluidics

(2013) Staffan L. Sjostrom et al.   LAB ON A CHIP

Picoliter Cell Lysate Assays in Microfluidic Droplet Compartments for Directed Enzyme Evolution

(2012) Balint Kintses et al.   CHEMISTRY & BIOLOGY

Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library

(2011) A. G. Sandstrom et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fluorescence Activated Cell Sorting as a General Ultra-High-Throughput Screening Method for Directed Evolution of Glycosyltransferases

(2010) Guangyu Yang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

(2010) J. J. Agresti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility

(2009) Garrett M. Morris et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Directed Evolution of Enantioselective Enzymes: An Unconventional Approach to Asymmetric Catalysis in Organic Chemistry

(2009) Manfred T. Reetz   JOURNAL OF ORGANIC CHEMISTRY

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

(2009) Jean-Christophe Baret et al.   LAB ON A CHIP

Enzyme engineering for enantioselectivity: from trial-and-error to rational design?

(2009) Linda G. Otten et al.   TRENDS IN BIOTECHNOLOGY