Mass spectrometry informs the structure and dynamics of membrane proteins involved in lipid and drug transport

A ‘Build and Retrieve’ methodology to simultaneously solve cryo-EM structures of membrane proteins

(2021) Chih-Chia Su et al.   NATURE METHODS

The antibiotic darobactin mimics a β-strand to inhibit outer membrane insertase

(2021) Hundeep Kaur et al.   NATURE

Scratching the surface: native mass spectrometry of peripheral membrane protein complexes

(2020) Cagla Sahin et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Hydrogen-Deuterium Exchange Mass-Spectrometry of Secondary Active Transporters: From Structural Dynamics to Molecular Mechanisms

(2020) Moshe Giladi et al.   Frontiers in Pharmacology

Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein

(2020) Alexandra C. Walls et al.   CELL

Combining native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteins

(2020) Joseph Gault et al.   NATURE METHODS

A glimpse into the molecular mechanism of integral membrane proteins through hydrogen–deuterium exchange mass spectrometry

(2020) Chloe Martens et al.   PROTEIN SCIENCE

Thanatin Impairs Lipopolysaccharide Transport Complex Assembly by Targeting LptC–LptA Interaction and Decreasing LptA Stability

(2020) Elisabete C. C. M. Moura et al.   Frontiers in Microbiology

Tackling antimicrobial resistance in the COVID-19 pandemic

(2020) Haileyesus Getahun et al.   BULLETIN OF THE WORLD HEALTH ORGANIZATION

High-resolution views of lipopolysaccharide translocation driven by ABC transporters MsbA and LptB2FGC

(2020) François Thélot et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Assembly and regulation of the chlorhexidine-specific efflux pump AceI

(2020) Jani Reddy Bolla et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly

(2020) Longfei Wang et al.   MOLECULAR CELL

Dynamics of an LPS translocon induced by substrate and an antimicrobial peptide

(2020) Francesco Fiorentino et al.   Nature Chemical Biology

Structural mass spectrometry comes of age: new insight into protein structure, function and interactions

(2019) Timothy M. Allison et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Mass spectrometry: From plasma proteins to mitochondrial membranes

(2019) Carol V. Robinson   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Concept of an Ideal Antibiotic: Implications for Drug Design

(2019) Márió Gajdács   MOLECULES

Membrane Protein–Lipid Interactions Probed Using Mass Spectrometry

(2019) Jani Reddy Bolla et al.   Annual Review of Biochemistry

Hydrogen-deuterium exchange mass spectrometry of membrane proteins in lipid nanodiscs

(2019) Michelle Redhair et al.   CHEMISTRY AND PHYSICS OF LIPIDS

Mass spectrometry—A versatile tool for characterising the lipid environment of membrane protein assemblies

(2019) Melissa Frick et al.   CHEMISTRY AND PHYSICS OF LIPIDS

Membrane protein dynamics studied by X-ray lasers – or why only time will tell

(2019) Jörg Standfuss   CURRENT OPINION IN STRUCTURAL BIOLOGY

Short-chain diamines are the physiological substrates of PACE family efflux pumps

(2019) Karl A. Hassan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Lpt ABC transporter for lipopolysaccharide export to the cell surface

(2019) Paola Sperandeo et al.   RESEARCH IN MICROBIOLOGY

Lipid trafficking across the Gram-negative cell envelope

(2019) Rahul Shrivastava et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A new antibiotic selectively kills Gram-negative pathogens

(2019) Yu Imai et al.   NATURE

A Peptidomimetic Antibiotic Interacts with the Periplasmic Domain of LptD from Pseudomonas aeruginosa

(2018) Gloria Andolina et al.   ACS Chemical Biology

Pharmacogenomics of GPCR Drug Targets

(2018) Alexander S. Hauser et al.   CELL

Mass spectrometry-enabled structural biology of membrane proteins

(2018) Antonio N. Calabrese et al.   METHODS

Unexplored therapeutic opportunities in the human genome

(2018) Tudor I. Oprea et al.   NATURE REVIEWS DRUG DISCOVERY

Multidrug efflux pumps: structure, function and regulation

(2018) Dijun Du et al.   NATURE REVIEWS MICROBIOLOGY

Pacing across the membrane: the novel PACE family of efflux pumps is widespread in Gram-negative pathogens

(2018) Karl A. Hassan et al.   RESEARCH IN MICROBIOLOGY

Lipopolysaccharide is transported to the cell surface by a membrane-to-membrane protein bridge

(2018) David J. Sherman et al.   SCIENCE

Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry

(2018) Dror S. Chorev et al.   SCIENCE

Thanatin targets the intermembrane protein complex required for lipopolysaccharide transport in Escherichia coli

(2018) Stefan U. Vetterli et al.   Science Advances

The role played by drug efflux pumps in bacterial multidrug resistance

(2017) Mohsen Chitsaz et al.   Essays in Biochemistry

The lipopolysaccharide transport (Lpt) machinery: A nonconventional transporter for lipopolysaccharide assembly at the outer membrane of Gram-negative bacteria

(2017) Paola Sperandeo et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Recent developments in solution nuclear magnetic resonance (NMR)-based molecular biology

(2017) Joshua J. Ziarek et al.   JOURNAL OF MOLECULAR MEDICINE-JMM

Structural basis of MsbA-mediated lipopolysaccharide transport

(2017) Wei Mi et al.   NATURE

The drug-resistant bacteria that pose the greatest health threats

(2017) Cassandra Willyard   NATURE

Structural basis for lipopolysaccharide extraction by ABC transporter LptB2FG

(2017) Qingshan Luo et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural and functional insights into the lipopolysaccharide ABC transporter LptB2FG

(2017) Haohao Dong et al.   Nature Communications

Structural basis for lipopolysaccharide extraction by ABC transporter LptB2FG

(2017) Qingshan Luo et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Lipopolysaccharide transport and assembly at the outer membrane: the PEZ model

(2016) Suguru Okuda et al.   NATURE REVIEWS MICROBIOLOGY

Single-Particle Cryo-EM at Crystallographic Resolution

(2015) Yifan Cheng   CELL

Structure, mechanism and cooperation of bacterial multidrug transporters

(2015) Dijun Du et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

AcrB drug-binding pocket substitution confers clinically relevant resistance and altered substrate specificity

(2015) Jessica M. A. Blair et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Lipopolysaccharide is Inserted into the Outer Membrane through An Intramembrane Hole, A Lumen Gate, and the Lateral Opening of LptD

(2015) Yinghong Gu et al.   STRUCTURE

Homologs of the Acinetobacter baumannii AceI Transporter Represent a New Family of Bacterial Multidrug Efflux Systems

(2015) Karl A. Hassan et al.   mBio

RND-type drug efflux pumps from Gram-negative bacteria: molecular mechanism and inhibition

(2015) Henrietta Venter et al.   Frontiers in Microbiology

LptE binds to and alters the physical state of LPS to catalyze its assembly at the cell surface

(2014) Goran Malojčić et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Extracellular Vesicles: A Platform for the Structure Determination of Membrane Proteins by Cryo-EM

(2014) Tzviya Zeev-Ben-Mordehai et al.   STRUCTURE

LptA Assembles into Rod-Like Oligomers Involving Disorder-to-Order Transitions

(2013) Carlo Santambrogio et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Transcriptomic and biochemical analyses identify a family of chlorhexidine efflux proteins

(2013) K. A. Hassan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Regulated Assembly of the Transenvelope Protein Complex Required for Lipopolysaccharide Export

(2012) Elizaveta Freinkman et al.   BIOCHEMISTRY

Wzx flippase-mediated membrane translocation of sugar polymer precursors in bacteria

(2012) Salim T. Islam et al.   ENVIRONMENTAL MICROBIOLOGY

New Insights into the Lpt Machinery for Lipopolysaccharide Transport to the Cell Surface: LptA-LptC Interaction and LptA Stability as Sensors of a Properly Assembled Transenvelope Complex

(2010) P. Sperandeo et al.   JOURNAL OF BACTERIOLOGY

Computational analysis of membrane proteins: the largest class of drug targets

(2009) Yalini Arinaminpathy et al.   DRUG DISCOVERY TODAY

Biophysical dissection of membrane proteins

(2009) Stephen H. White   NATURE

Novel Structure of the Conserved Gram-Negative Lipopolysaccharide Transport Protein A and Mutagenesis Analysis

(2008) Michael D.L. Suits et al.   JOURNAL OF MOLECULAR BIOLOGY