Higher-order organization of biomolecular condensates

p53 is a central regulator driving neurodegeneration caused by C9orf72 poly(PR)

(2021) Maya Maor-Nof et al.   CELL

ZSP‐1 is a Z granule surface protein required for Z granule fluidity and germline immortality in Caenorhabditis elegans

(2021) Gang Wan et al.   EMBO JOURNAL

FUS and TDP-43 Phases in Health and Disease

(2021) Bede Portz et al.   TRENDS IN BIOCHEMICAL SCIENCES

p62/SQSTM1-droplet serves as a platform for autophagosome formation and anti-oxidative stress response

(2021) Shun Kageyama et al.   Nature Communications

Self‐assembly of multi‐component mitochondrial nucleoids via phase separation

(2021) Marina Feric et al.   EMBO JOURNAL

Open Access: A Role for p53 in c9ALS/FTD?

(2021) Charlotte M. Fare et al.   TRENDS IN GENETICS

Sequence-encoded and composition-dependent protein-RNA interactions control multiphasic condensate morphologies

(2021) Taranpreet Kaur et al.   Nature Communications

In vivo reconstitution finds multivalent RNA-RNA interactions as drivers of mesh-like condensates

(2021) Weirui Ma et al.   eLife

Combating deleterious phase transitions in neurodegenerative disease

(2021) April L. Darling et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

The role of liquid–liquid phase separation in regulating enzyme activity

(2021) Brian G. O'Flynn et al.   CURRENT OPINION IN CELL BIOLOGY

(Dis)Solving the problem of aberrant protein states

(2021) Charlotte M. Fare et al.   Disease Models & Mechanisms

p53 mediates target gene association with nuclear speckles for amplified RNA expression

(2021) Katherine A. Alexander et al.   MOLECULAR CELL

RNA Droplets

(2020) Kevin Rhine et al.   Annual Review of Biophysics

Physical Principles Underlying the Complex Biology of Intracellular Phase Transitions

(2020) Jeong-Mo Choi et al.   Annual Review of Biophysics

Liquid-Liquid Phase Separation of Histone Proteins in Cells: Role in Chromatin Organization

(2020) Anisha Shakya et al.   BIOPHYSICAL JOURNAL

Modulation of RNA Condensation by the DEAD-Box Protein eIF4A

(2020) Devin Tauber et al.   CELL

UBAP2L Forms Distinct Cores that Act in Nucleating Stress Granules Upstream of G3BP1

(2020) Luca Cirillo et al.   CURRENT BIOLOGY

Regulated changes in material properties underlie centrosome disassembly during mitotic exit

(2020) Matthäus Mittasch et al.   JOURNAL OF CELL BIOLOGY

Mouse Heterochromatin Adopts Digital Compaction States without Showing Hallmarks of HP1-Driven Liquid-Liquid Phase Separation

(2020) Fabian Erdel et al.   MOLECULAR CELL

Proteome-wide observation of the phenomenon of life on the edge of solubility

(2020) Giulia Vecchi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Valence and patterning of aromatic residues determine the phase behavior of prion-like domains

(2020) Erik W. Martin et al.   SCIENCE

C9orf72 arginine-rich dipeptide repeat proteins disrupt karyopherin-mediated nuclear import

(2020) Lindsey R Hayes et al.   eLife

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization

(2020) David W. Sanders et al.   CELL

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules

(2020) Peiguo Yang et al.   CELL

RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation

(2020) Jordina Guillén-Boixet et al.   CELL

Just Took a DNA Test, Turns Out 100% Not That Phase

(2020) Aaron D. Gitler et al.   MOLECULAR CELL

Phase separation directs ubiquitination of gene-body nucleosomes

(2020) Laura D. Gallego et al.   NATURE

Composition-dependent thermodynamics of intracellular phase separation

(2020) Joshua A. Riback et al.   NATURE

Liquid network connectivity regulates the stability and composition of biomolecular condensates with many components

(2020) Jorge R. Espinosa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Liquid-liquid phase separation drives skin barrier formation

(2020) Felipe Garcia Quiroz et al.   SCIENCE

Phase transition of RNA−protein complexes into ordered hollow condensates

(2020) Ibraheem Alshareedah et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nucleated transcriptional condensates amplify gene expression

(2020) Ming-Tzo Wei et al.   NATURE CELL BIOLOGY

Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics

(2020) Timo Rey et al.   NATURE CELL BIOLOGY

C9orf72 poly(GR) aggregation induces TDP-43 proteinopathy

(2020) Casey N. Cook et al.   Science Translational Medicine

Tunable multiphase dynamics of arginine and lysine liquid condensates

(2020) Rachel S. Fisher et al.   Nature Communications

Condensed Chromatin Behaves like a Solid on the Mesoscale In Vitro and in Living Cells

(2020) Hilmar Strickfaden et al.   CELL

Phase Separation as a Missing Mechanism for Interpretation of Disease Mutations

(2020) Brian Tsang et al.   CELL

Biochemical Timekeeping Via Reentrant Phase Transitions

(2020) Bede Portz et al.   JOURNAL OF MOLECULAR BIOLOGY

Protein condensates as aging Maxwell fluids

(2020) Louise Jawerth et al.   SCIENCE

Nuclear Import Receptors Directly Bind to Arginine-Rich Dipeptide Repeat Proteins and Suppress Their Pathological Interactions

(2020) Saskia Hutten et al.   Cell Reports

Multicolour single-molecule tracking of mRNA interactions with RNP granules

(2019) Stephanie L. Moon et al.   NATURE CELL BIOLOGY

Aberrant Phase Transitions: Side Effects and Novel Therapeutic Strategies in Human Disease

(2019) Veronica Verdile et al.   Frontiers in Genetics

Template-directed RNA polymerization and enhanced ribozyme catalysis inside membraneless compartments formed by coacervates

(2019) Raghav R. Poudyal et al.   Nature Communications

Heterochromatin anomalies and double-stranded RNA accumulation underlie C9orf72 poly(PR) toxicity

(2019) Yong-Jie Zhang et al.   SCIENCE

Stoichiometry controls activity of phase-separated clusters of actin signaling proteins

(2019) Lindsay B. Case et al.   SCIENCE

RNA Binding Antagonizes Neurotoxic Phase Transitions of TDP-43

(2019) Jacob R. Mann et al.   NEURON

A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos

(2019) Andrea Putnam et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

Pollen Cell Wall Patterns Form from Modulated Phases

(2019) Asja Radja et al.   CELL

Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality

(2019) Sethuramasundaram Pitchiaya et al.   MOLECULAR CELL

Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties

(2019) Steven Boeynaems et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Chronic optogenetic induction of stress granules is cytotoxic and reveals the evolution of ALS-FTD pathology

(2019) Peipei Zhang et al.   eLife

Histone Modifications Regulate Chromatin Compartmentalization by Contributing to a Phase Separation Mechanism

(2019) Liang Wang et al.   MOLECULAR CELL

DEAD-box ATPases are global regulators of phase-separated organelles

(2019) Maria Hondele et al.   NATURE

Phospho-dependent phase separation of FMRP and CAPRIN1 recapitulates regulation of translation and deadenylation

(2019) Tae Hun Kim et al.   SCIENCE

RRM adjacent TARDBP mutations disrupt RNA binding and enhance TDP-43 proteinopathy

(2019) Han-Jou Chen et al.   BRAIN

Organization of Chromatin by Intrinsic and Regulated Phase Separation

(2019) Bryan A. Gibson et al.   CELL

Nucleo-cytoplasmic Partitioning of ARF Proteins Controls Auxin Responses in Arabidopsis thaliana

(2019) Samantha K. Powers et al.   MOLECULAR CELL

Properties of Stress Granule and P-Body Proteomes

(2019) Ji-Young Youn et al.   MOLECULAR CELL

HP1 reshapes nucleosome core to promote phase separation of heterochromatin

(2019) S. Sanulli et al.   NATURE

Stress granules and neurodegeneration

(2019) Benjamin Wolozin et al.   NATURE REVIEWS NEUROSCIENCE

Cytoplasmic DAXX drives SQSTM1/p62 phase condensation to activate Nrf2-mediated stress response

(2019) Yi Yang et al.   Nature Communications

LASSI: A lattice model for simulating phase transitions of multivalent proteins

(2019) Jeong-Mo Choi et al.   PLoS Computational Biology

Nucleolar dynamics and interactions with nucleoplasm in living cells

(2019) Christina M Caragine et al.   eLife

P-Bodies: Composition, Properties, and Functions

(2018) Yang Luo et al.   BIOCHEMISTRY

Surface Properties Determining Passage Rates of Proteins through Nuclear Pores

(2018) Steffen Frey et al.   CELL

Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules

(2018) Sebastian Markmiller et al.   CELL

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains

(2018) Lin Guo et al.   CELL

Reversible induction of TDP-43 granules in cortical neurons after traumatic injury

(2018) Diana Wiesner et al.   EXPERIMENTAL NEUROLOGY

Single-molecule imaging reveals dynamic biphasic partition of RNA-binding proteins in stress granules

(2018) Benedikt Niewidok et al.   JOURNAL OF CELL BIOLOGY

Amyloid assembly and disassembly

(2018) Edward Chuang et al.   JOURNAL OF CELL SCIENCE

ZNFX-1 Functions within Perinuclear Nuage to Balance Epigenetic Signals

(2018) Takao Ishidate et al.   MOLECULAR CELL

Controlling compartmentalization by non-membrane-bound organelles

(2018) Richard J. Wheeler et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Physical principles of intracellular organization via active and passive phase transitions

(2018) Joel Berry et al.   REPORTS ON PROGRESS IN PHYSICS

RNA buffers the phase separation behavior of prion-like RNA binding proteins

(2018) Shovamayee Maharana et al.   SCIENCE

DNA-induced liquid phase condensation of cGAS activates innate immune signaling

(2018) Mingjian Du et al.   SCIENCE

Phase separation of a yeast prion protein promotes cellular fitness

(2018) Titus M. Franzmann et al.   SCIENCE

Organization and Function of Non-dynamic Biomolecular Condensates

(2018) Jeffrey B. Woodruff et al.   TRENDS IN BIOCHEMICAL SCIENCES

Protein Phase Separation: A New Phase in Cell Biology

(2018) Steven Boeynaems et al.   TRENDS IN CELL BIOLOGY

Self-interaction of NPM1 modulates multiple mechanisms of liquid–liquid phase separation

(2018) Diana M. Mitrea et al.   Nature Communications

A compartmentalized signaling network mediates crossover control in meiosis

(2018) Liangyu Zhang et al.   eLife

Pi-Pi contacts are an overlooked protein feature relevant to phase separation

(2018) Robert McCoy Vernon et al.   eLife

A New Lens for RNA Localization: Liquid-Liquid Phase Separation

(2018) Erin M. Langdon et al.   Annual Review of Microbiology

The molecular language of membraneless organelles

(2018) Edward Gomes et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Who's In and Who's Out—Compositional Control of Biomolecular Condensates

(2018) Jonathon A. Ditlev et al.   JOURNAL OF MOLECULAR BIOLOGY

Cancer Mutations of the Tumor Suppressor SPOP Disrupt the Formation of Active, Phase-Separated Compartments

(2018) Jill J. Bouchard et al.   MOLECULAR CELL

Poly(ADP-Ribose) Prevents Pathological Phase Separation of TDP-43 by Promoting Liquid Demixing and Stress Granule Localization

(2018) Leeanne McGurk et al.   MOLECULAR CELL

Spatiotemporal regulation of liquid-like condensates in epigenetic inheritance

(2018) Gang Wan et al.   NATURE

Phase transitioned nuclear Oskar promotes cell division of Drosophila primordial germ cells

(2018) Kathryn E Kistler et al.   eLife

Ubiquitin Regulation: The Histone Modifying Enzyme′s Story

(2018) Jianlin Wang et al.   Cells

Differences between acute and chronic stress granules, and how these differences may impact function in human disease

(2018) Lucas C. Reineke et al.   BIOCHEMICAL PHARMACOLOGY

A Membraneless Organelle Associated with the Endoplasmic Reticulum Enables 3′UTR-Mediated Protein-Protein Interactions

(2018) Weirui Ma et al.   CELL

Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds

(2018) Dan Bracha et al.   CELL

3′ UTRs in the Eye of the TIGER

(2018) Bede Portz et al.   DEVELOPMENTAL CELL

Remodeling of the endoplasmic reticulum in Caenorhabditis elegans oocytes is regulated by CGH-1

(2018) Shaughna Langerak et al.   GENESIS

TDP-43 and RNA form amyloid-like myo-granules in regenerating muscle

(2018) Thomas O. Vogler et al.   NATURE

Membraneless nuclear organelles and the search for phases within phases

(2018) Iain A. Sawyer et al.   Wiley Interdisciplinary Reviews-RNA

P-Body Purification Reveals the Condensation of Repressed mRNA Regulons

(2017) Arnaud Hubstenberger et al.   MOLECULAR CELL

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Eye patches: Protein assembly of index-gradient squid lenses

(2017) J. Cai et al.   SCIENCE

The synaptonemal complex has liquid crystalline properties and spatially regulates meiotic recombination factors

(2017) Ofer Rog et al.   eLife

A de novo 10p11.23-p12.1 deletion recapitulates the phenotype observed inWACmutations and strengthens the role ofWACin intellectual disability and behavior disorders

(2016) Fatma Abdelhedi et al.   AMERICAN JOURNAL OF MEDICAL GENETICS PART A

Architectural RNAs (arcRNAs): A class of long noncoding RNAs that function as the scaffold of nuclear bodies

(2016) Takeshi Chujo et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

Prion-like domains as epigenetic regulators, scaffolds for subcellular organization, and drivers of neurodegenerative disease

(2016) Zachary M. March et al.   BRAIN RESEARCH

Amyloid-like Self-Assembly of a Cellular Compartment

(2016) Elvan Boke et al.   CELL

ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure

(2016) Saumya Jain et al.   CELL

Compositional Control of Phase-Separated Cellular Bodies

(2016) Salman F. Banani et al.   CELL

Coexisting Liquid Phases Underlie Nucleolar Subcompartments

(2016) Marina Feric et al.   CELL

Phase separation in biology; functional organization of a higher order

(2016) Diana M. Mitrea et al.   Cell Communication and Signaling

A glass menagerie of low complexity sequences

(2016) Randal Halfmann   CURRENT OPINION IN STRUCTURAL BIOLOGY

Higher‐order oligomerization promotes localization of SPOP to liquid nuclear speckles

(2016) Melissa R Marzahn et al.   EMBO JOURNAL

Structural, super-resolution microscopy analysis of paraspeckle nuclear body organization

(2016) Jason A. West et al.   JOURNAL OF CELL BIOLOGY

Phasing in and out

(2016) James Shorter   Nature Chemistry

Membraneless organelles can melt nucleic acid duplexes and act as biomolecular filters

(2016) Timothy J. Nott et al.   Nature Chemistry

Structural mechanism for the recognition and ubiquitination of a single nucleosome residue by Rad6–Bre1

(2016) Laura D. Gallego et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Phase separation of signaling molecules promotes T cell receptor signal transduction

(2016) Xiaolei Su et al.   SCIENCE

Principles and Properties of Stress Granules

(2016) David S.W. Protter et al.   TRENDS IN CELL BIOLOGY

In Vivo Formation of Vacuolated Multi-phase Compartments Lacking Membranes

(2016) Hermann Broder Schmidt et al.   Cell Reports

Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3

(2016) Jarrett Smith et al.   eLife

Distinct stages in stress granule assembly and disassembly

(2016) Joshua R Wheeler et al.   eLife

Phase Separation by Low Complexity Domains Promotes Stress Granule Assembly and Drives Pathological Fibrillization

(2015) Amandine Molliex et al.   CELL

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

(2015) Avinash Patel et al.   CELL

WACloss-of-function mutations cause a recognisable syndrome characterised by dysmorphic features, developmental delay and hypotonia and recapitulate 10p11.23 microdeletion syndrome

(2015) Cori DeSanto et al.   JOURNAL OF MEDICAL GENETICS

An acetylation switch controls TDP-43 function and aggregation propensity

(2015) Todd J. Cohen et al.   Nature Communications

The Selective Autophagy Receptor p62 Forms a Flexible Filamentous Helical Scaffold

(2015) Rodolfo Ciuffa et al.   Cell Reports

The Nucleosome Acidic Patch Regulates the H2B K123 Monoubiquitylation Cascade and Transcription Elongation in Saccharomyces cerevisiae

(2015) Christine E. Cucinotta et al.   PLoS Genetics

Liquid-Liquid Phase Separation in Biology

(2014) Anthony A. Hyman et al.   Annual Review of Cell and Developmental Biology

Transgenerational Epigenetic Inheritance: Myths and Mechanisms

(2014) Edith Heard et al.   CELL

The quantified cell

(2014) Avi Flamholz et al.   MOLECULAR BIOLOGY OF THE CELL

The metazoan protein disaggregase and amyloid depolymerase system

(2014) Mariana P Torrente et al.   Prion

Bidirectional transcripts of the expanded C9orf72 hexanucleotide repeat are translated into aggregating dipeptide repeat proteins

(2013) Kohji Mori et al.   ACTA NEUROPATHOLOGICA

GRSF1 Regulates RNA Processing in Mitochondrial RNA Granules

(2013) Alexis A. Jourdain et al.   Cell Metabolism

Translation Repressors, an RNA Helicase, and Developmental Cues Control RNP Phase Transitions during Early Development

(2013) Arnaud Hubstenberger et al.   DEVELOPMENTAL CELL

Stress granules as crucibles of ALS pathogenesis

(2013) Yun R. Li et al.   JOURNAL OF CELL BIOLOGY

Predicting phase behavior in multicomponent mixtures

(2013) William M. Jacobs et al.   JOURNAL OF CHEMICAL PHYSICS

Mutations in prion-like domains in hnRNPA2B1 and hnRNPA1 cause multisystem proteinopathy and ALS

(2013) Hong Joo Kim et al.   NATURE

Paraspeckle formation during the biogenesis of long non-coding RNAs

(2013) Takao Naganuma et al.   RNA Biology

Bridging the transgenerational gap with epigenetic memory

(2013) Jana P. Lim et al.   TRENDS IN GENETICS

The tip of the iceberg: RNA-binding proteins with prion-like domains in neurodegenerative disease

(2012) Oliver D. King et al.   BRAIN RESEARCH

The Dual Organization of P-bodies Revealed by Immunoelectron Microscopy and Electron Tomography

(2012) Nicolas Cougot et al.   JOURNAL OF MOLECULAR BIOLOGY

Transient Sequestration of TORC1 into Stress Granules during Heat Stress

(2012) Terunao Takahara et al.   MOLECULAR CELL

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

A nuclear Argonaute promotes multigenerational epigenetic inheritance and germline immortality

(2012) Bethany A. Buckley et al.   NATURE

RNA catalysis through compartmentalization

(2012) Christopher A. Strulson et al.   Nature Chemistry

Small Heat Shock Proteins Potentiate Amyloid Dissolution by Protein Disaggregases from Yeast and Humans

(2012) Martin L. Duennwald et al.   PLOS BIOLOGY

Patchy colloids: state of the art and perspectives

(2011) Emanuela Bianchi et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

The Mammalian Disaggregase Machinery: Hsp110 Synergizes with Hsp70 and Hsp40 to Catalyze Protein Disaggregation and Reactivation in a Cell-Free System

(2011) James Shorter   PLoS One

Prion-like disorders: blurring the divide between transmissibility and infectivity

(2010) M. Cushman et al.   JOURNAL OF CELL SCIENCE

In vivo kinetics of U4/U6·U5 tri-snRNP formation in Cajal bodies

(2010) Ivan Novotný et al.   MOLECULAR BIOLOGY OF THE CELL

The Nucleolus under Stress

(2010) Séverine Boulon et al.   MOLECULAR CELL

A simple approach to polymer mixture miscibility

(2010) J. S. Higgins et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES

RAD6-Mediated Transcription-Coupled H2B Ubiquitylation Directly Stimulates H3K4 Methylation in Human Cells

(2009) Jaehoon Kim et al.   CELL

Unravelling the ultrastructure of stress granules and associated P-bodies in human cells

(2009) S. Souquere et al.   JOURNAL OF CELL SCIENCE

Eukaryotic Stress Granules: The Ins and Outs of Translation

(2009) J. Ross Buchan et al.   MOLECULAR CELL

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

Transmission Dynamics of Heritable Silencing Induced by Double-Stranded RNA in Caenorhabditis elegans

(2008) Rosa M. Alcazar et al.   GENETICS

H2B Ubiquitylation Plays a Role in Nucleosome Dynamics during Transcription Elongation

(2008) Alastair B. Fleming et al.   MOLECULAR CELL

Formation of stress granules inhibits apoptosis by suppressing stress-responsive MAPK pathways

(2008) Kyoko Arimoto et al.   NATURE CELL BIOLOGY