Optimized CRISPR-mediated gene knockin reveals FOXP3-independent maintenance of human Treg identity

Conserved human effector Treg cell transcriptomic and epigenetic signature in arthritic joint inflammation

(2021) Gerdien Mijnheer et al.   Nature Communications

CoMeBack: DNA methylation array data analysis for co-methylated regions

(2020) Evan Gatev et al.   BIOINFORMATICS

Control of regulatory T‐cell differentiation and function by T‐cell receptor signalling and Foxp3 transcription factor complexes

(2020) Masahiro Ono   IMMUNOLOGY

Regulatory T Cell-Specific Epigenomic Region Variants Are a Key Determinant of Susceptibility to Common Autoimmune Diseases

(2020) Naganari Ohkura et al.   IMMUNITY

GPA33: A Marker to Identify Stable Human Regulatory T Cells

(2020) Rianne Opstelten et al.   JOURNAL OF IMMUNOLOGY

CRISPR-based gene editing enables FOXP3 gene repair in IPEX patient cells

(2020) M. Goodwin et al.   Science Advances

Regulatory T cells: Master thieves of the immune system

(2020) Billur Akkaya et al.   CELLULAR IMMUNOLOGY

PD-1 restraint of regulatory T cell suppressive activity is critical for immune tolerance

(2020) Catherine L. Tan et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Functional CRISPR dissection of gene networks controlling human regulatory T cell identity

(2020) Kathrin Schumann et al.   NATURE IMMUNOLOGY

Functional effects of chimeric antigen receptor co-receptor signaling domains in human regulatory T cells

(2020) Nicholas A. J. Dawson et al.   Science Translational Medicine

Cross talk between human regulatory T cells and antigen‐presenting cells: Lessons for clinical applications

(2020) Christine M Wardell et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

Dynamics and competition of CRISPR–Cas9 ribonucleoproteins and AAV donor-mediated NHEJ, MMEJ and HDR editing

(2020) Ya-Wen Fu et al.   NUCLEIC ACIDS RESEARCH

A novel TNFR2 agonist antibody expands highly potent regulatory T cells

(2020) Heather Torrey et al.   Science Signaling

Methodologies for Improving HDR Efficiency

(2019) Mingjie Liu et al.   Frontiers in Genetics

A Mutation in the Transcription Factor Foxp3 Drives T Helper 2 Effector Function in Regulatory T Cells

(2019) Frédéric Van Gool et al.   IMMUNITY

PTC-bearing mRNA elicits a genetic compensation response via Upf3a and COMPASS components

(2019) Zhipeng Ma et al.   NATURE

Genetic compensation triggered by mutant mRNA degradation

(2019) Mohamed A. El-Brolosy et al.   NATURE

TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases

(2019) Bi-Huei Yang et al.   Cell Reports

Next-generation regulatory T cell therapy

(2019) Leonardo M. R. Ferreira et al.   NATURE REVIEWS DRUG DISCOVERY

Biological plasticity rescues target activity in CRISPR knock outs

(2019) Arne H. Smits et al.   NATURE METHODS

CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation

(2019) Rubina Tuladhar et al.   Nature Communications

Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency

(2019) David N. Nguyen et al.   NATURE BIOTECHNOLOGY

Proteomic Analyses of Human Regulatory T Cells Reveal Adaptations in Signaling Pathways that Protect Cellular Identity

(2018) Eloy Cuadrado et al.   IMMUNITY

Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells

(2018) Akiko Seki et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing

(2018) Xin Chen et al.   JOURNAL OF IMMUNOLOGY

Scaffolds that mimic antigen-presenting cells enable ex vivo expansion of primary T cells

(2018) Alexander S Cheung et al.   NATURE BIOTECHNOLOGY

Specific or not specific recruitment of DNMTs for DNA methylation, an epigenetic dilemma

(2018) Eric Hervouet et al.   Clinical Epigenetics

CD137+CD154− Expression As a Regulatory T Cell (Treg)-Specific Activation Signature for Identification and Sorting of Stable Human Tregs from In Vitro Expansion Cultures

(2018) Anna Nowak et al.   Frontiers in Immunology

Reprogramming human T cell function and specificity with non-viral genome targeting

(2018) Theodore L. Roth et al.   NATURE

Heavy-tailed prior distributions for sequence count data: removing the noise and preserving large differences

(2018) Anqi Zhu et al.   BIOINFORMATICS

Versatile and precise gene-targeting strategies for functional studies in mammalian cell lines

(2017) M. Wassef et al.   METHODS

Integration of a CD19 CAR into the TCR Alpha Chain Locus Streamlines Production of Allogeneic Gene-Edited CAR T Cells

(2017) Daniel T. MacLeod et al.   MOLECULAR THERAPY

Engineering HIV-Resistant, Anti-HIV Chimeric Antigen Receptor T Cells

(2017) Malika Hale et al.   MOLECULAR THERAPY

Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection

(2017) Justin Eyquem et al.   NATURE

Identification of methylation haplotype blocks aids in deconvolution of heterogeneous tissue samples and tumor tissue-of-origin mapping from plasma DNA

(2017) Shicheng Guo et al.   NATURE GENETICS

Impact of cytosine methylation on DNA binding specificities of human transcription factors

(2017) Yimeng Yin et al.   SCIENCE

CRISPR-Cas9-mediated multiplex gene editing in CAR-T cells

(2016) Xiaojuan Liu et al.   CELL RESEARCH

Multiplex Genome Editing to Generate Universal CAR T Cells Resistant to PD1 Inhibition

(2016) Jiangtao Ren et al.   CLINICAL CANCER RESEARCH

A Regulatory T-Cell Gene Signature Is a Specific and Sensitive Biomarker to Identify Children With New-Onset Type 1 Diabetes

(2016) Anne M. Pesenacker et al.   DIABETES

The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements

(2016) Claudio Procaccini et al.   IMMUNITY

Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives

(2016) Fabian Oceguera-Yanez et al.   METHODS

High Efficiency CRISPR/Cas9-mediated Gene Editing in Primary Human T-cells Using Mutant Adenoviral E4orf6/E1b55k “Helper” Proteins

(2016) Kamila S Gwiazda et al.   MOLECULAR THERAPY

Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9

(2016) Dominik Paquet et al.   NATURE

CRISPR/Cas9 β-globin gene targeting in human haematopoietic stem cells

(2016) Daniel P. Dever et al.   NATURE

Guidance of regulatory T cell development by Satb1-dependent super-enhancer establishment

(2016) Yohko Kitagawa et al.   NATURE IMMUNOLOGY

Discarded Human Thymus Is a Novel Source of Stable and Long-Lived Therapeutic Regulatory T Cells

(2015) I. E. Dijke et al.   AMERICAN JOURNAL OF TRANSPLANTATION

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

(2015) Ayal Hendel et al.   NATURE BIOTECHNOLOGY

Highly efficient homology-driven genome editing in human T cells by combining zinc-finger nuclease mRNA and AAV6 donor delivery

(2015) Jianbin Wang et al.   NUCLEIC ACIDS RESEARCH

Generation of knock-in primary human T cells using Cas9 ribonucleoproteins

(2015) Kathrin Schumann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient modification of CCR5 in primary human hematopoietic cells using a megaTAL nuclease and AAV donor template

(2015) Blythe D. Sather et al.   Science Translational Medicine

Minfi: a flexible and comprehensive Bioconductor package for the analysis of Infinium DNA methylation microarrays

(2014) Martin J. Aryee et al.   BIOINFORMATICS

The enhancer and promoter landscape of human regulatory and conventional T-cell subpopulations

(2014) C. Schmidl et al.   BLOOD

Enhanced suppressor function of TIM-3+FoxP3+regulatory T cells

(2014) Anne-Sophie Gautron et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

Treg Cells Expressing the Coinhibitory Molecule TIGIT Selectively Inhibit Proinflammatory Th1 and Th17 Cell Responses

(2014) Nicole Joller et al.   IMMUNITY

Biallelic targeting of expressed genes in mouse embryonic stem cells using the Cas9 system

(2014) Yu Zhang et al.   METHODS

Interindividual variation in human T regulatory cells

(2014) A. Ferraro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Differential roles of epigenetic changes and Foxp3 expression in regulatory T cell-specific transcriptional regulation

(2014) H. Morikawa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome-wide DNA methylation analysis identifies hypomethylated genes regulated by FOXP3 in human regulatory T cells

(2013) Y. Zhang et al.   BLOOD

Foxp3+ T-regulatory cells require DNA methyltransferase 1 expression to prevent development of lethal autoimmunity

(2013) L. Wang et al.   BLOOD

A data-driven approach to preprocessing Illumina 450K methylation array data

(2013) Ruth Pidsley et al.   BMC GENOMICS

BACH2 represses effector programs to stabilize Treg-mediated immune homeostasis

(2013) Rahul Roychoudhuri et al.   NATURE

mTORC1 couples immune signals and metabolic programming to establish Treg-cell function

(2013) Hu Zeng et al.   NATURE

Nr4a receptors are essential for thymic regulatory T cell development and immune homeostasis

(2013) Takashi Sekiya et al.   NATURE IMMUNOLOGY

Pathogenic conversion of Foxp3+ T cells into TH17 cells in autoimmune arthritis

(2013) Noriko Komatsu et al.   NATURE MEDICINE

Low-level processing of Illumina Infinium DNA Methylation BeadArrays

(2013) Timothy J. Triche et al.   NUCLEIC ACIDS RESEARCH

The sva package for removing batch effects and other unwanted variation in high-throughput experiments

(2012) Jeffrey T. Leek et al.   BIOINFORMATICS

STAR: ultrafast universal RNA-seq aligner

(2012) Alexander Dobin et al.   BIOINFORMATICS

A beta-mixture quantile normalization method for correcting probe design bias in Illumina Infinium 450 k DNA methylation data

(2012) Andrew E. Teschendorff et al.   BIOINFORMATICS

Functionally distinct subsets of human FOXP3+ Treg cells that phenotypically mirror effector Th cells

(2012) T. Duhen et al.   BLOOD

Foxp3 Exploits a Pre-Existent Enhancer Landscape for Regulatory T Cell Lineage Specification

(2012) Robert M. Samstein et al.   CELL

Molecular and biological role of the FOXP3 N-terminal domain in immune regulation by T regulatory/suppressor cells

(2012) Guoping Deng et al.   EXPERIMENTAL AND MOLECULAR PATHOLOGY

T Cell Receptor Stimulation-Induced Epigenetic Changes and Foxp3 Expression Are Independent and Complementary Events Required for Treg Cell Development

(2012) Naganari Ohkura et al.   IMMUNITY

CD2-mediated regulation of peripheral CD4+ CD25+regulatory T-cell apoptosis accompanied by down-regulation of Bim

(2012) Yuji Kashiwakura et al.   IMMUNOLOGY

A multiply redundant genetic switch 'locks in' the transcriptional signature of regulatory T cells

(2012) Wenxian Fu et al.   NATURE IMMUNOLOGY

Transcription factor Foxp3 and its protein partners form a complex regulatory network

(2012) Dipayan Rudra et al.   NATURE IMMUNOLOGY

Molecular Mechanisms of Treg-Mediated T Cell Suppression

(2012) Angelika Schmidt et al.   Frontiers in Immunology

Molecular signatures database (MSigDB) 3.0

(2011) A. Liberzon et al.   BIOINFORMATICS

The Time is Crucial for Ex Vivo Expansion of T Regulatory Cells for Therapy

(2011) Natalia Marek et al.   CELL TRANSPLANTATION

An Essential Role of the Transcription Factor GATA-3 for the Function of Regulatory T Cells

(2011) Yunqi Wang et al.   IMMUNITY

GATA3 controls Foxp3+ regulatory T cell fate during inflammation in mice

(2011) Elizabeth A. Wohlfert et al.   JOURNAL OF CLINICAL INVESTIGATION

Identification of T helper type 1–like, Foxp3+ regulatory T cells in human autoimmune disease

(2011) Margarita Dominguez-Villar et al.   NATURE MEDICINE

Site-specific integration and tailoring of cassette design for sustainable gene transfer

(2011) Angelo Lombardo et al.   NATURE METHODS

Next-generation insights into regulatory T cells: expression profiling and FoxP3 occupancy in Human

(2011) Fabian Birzele et al.   NUCLEIC ACIDS RESEARCH

The nuclear orphan receptor Nr4a2 induces Foxp3 and regulates differentiation of CD4+ T cells

(2011) Takashi Sekiya et al.   Nature Communications

Comparison of Beta-value and M-value methods for quantifying methylation levels by microarray analysis

(2010) Pan Du et al.   BMC BIOINFORMATICS

The Ets-1 transcription factor controls the development and function of natural regulatory T cells

(2010) Enguerran Mouly et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Genome-Wide Identification of Human FOXP3 Target Genes in Natural Regulatory T Cells

(2010) T. J. Sadlon et al.   JOURNAL OF IMMUNOLOGY

Methylation matters: binding of Ets-1 to the demethylated Foxp3 gene contributes to the stabilization of Foxp3 expression in regulatory T cells

(2010) Julia K. Polansky et al.   JOURNAL OF MOLECULAR MEDICINE-JMM

Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell Identities

(2010) Sven Heinz et al.   MOLECULAR CELL

Selective expression of latency-associated peptide (LAP) and IL-1 receptor type I/II (CD121a/CD121b) on activated human FOXP3+ regulatory T cells allows for their purification from expansion cultures

(2009) D. Q. Tran et al.   BLOOD

Loss of FOXP3 expression in natural human CD4+CD25+regulatory T cells upon repetitivein vitrostimulation

(2009) Petra Hoffmann et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

Lineage-specific DNA methylation in T cells correlates with histone methylation and enhancer activity

(2009) C. Schmidl et al.   GENOME RESEARCH

Nuclear Factor-κB Modulates Regulatory T Cell Development by Directly Regulating Expression of Foxp3 Transcription Factor

(2009) Meixiao Long et al.   IMMUNITY

Development of Foxp3+ Regulatory T Cells Is Driven by the c-Rel Enhanceosome

(2009) Qingguo Ruan et al.   IMMUNITY

Functional Delineation and Differentiation Dynamics of Human CD4+ T Cells Expressing the FoxP3 Transcription Factor

(2009) Makoto Miyara et al.   IMMUNITY

c-Rel is required for the development of thymic Foxp3+CD4 regulatory T cells

(2009) Iwao Isomura et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Regulated and Multiple miRNA and siRNA Delivery Into Primary Cells by a Lentiviral Platform

(2009) Mario Amendola et al.   MOLECULAR THERAPY

Instability of the transcription factor Foxp3 leads to the generation of pathogenic memory T cells in vivo

(2009) Xuyu Zhou et al.   NATURE IMMUNOLOGY

FoxP3 maintains Treg unresponsiveness by selectively inhibiting the promoter DNA-binding activity of AP-1

(2008) S.-M. Lee et al.   BLOOD

Foxp3 Inhibits RORγt-mediatedIL-17AmRNA Transcription through Direct Interaction with RORγt

(2008) Kenji Ichiyama et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

TGF-β-induced Foxp3 inhibits TH17 cell differentiation by antagonizing RORγt function

(2008) Liang Zhou et al.   NATURE