Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells
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Title
Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells
Authors
Keywords
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Journal
JOURNAL OF EXPERIMENTAL MEDICINE
Volume 215, Issue 3, Pages 985-997
Publisher
Rockefeller University Press
Online
2018-02-07
DOI
10.1084/jem.20171626
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- Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes
- (2016) Van Trung Chu et al. BMC BIOTECHNOLOGY
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- (2016) Xiaojuan Liu et al. CELL RESEARCH
- Multiplex Genome Editing to Generate Universal CAR T Cells Resistant to PD1 Inhibition
- (2016) Jiangtao Ren et al. CLINICAL CANCER RESEARCH
- A CRISPR-Based Toolbox for Studying T Cell Signal Transduction
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- CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients
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- Inhibition of HIV-1 infection of primary CD4+ T-cells by gene editing of CCR5 using adenovirus-delivered CRISPR/Cas9
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- Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells
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- (2014) Randall J. Platt et al. CELL
- Development and Applications of CRISPR-Cas9 for Genome Engineering
- (2014) Patrick D. Hsu et al. CELL
- Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9
- (2014) Pankaj K. Mandal et al. Cell Stem Cell
- CCR5 Gene Disruption via Lentiviral Vectors Expressing Cas9 and Single Guided RNA Renders Cells Resistant to HIV-1 Infection
- (2014) Weiming Wang et al. PLoS One
- Cas9 as a versatile tool for engineering biology
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- Multiplex Genome Engineering Using CRISPR/Cas Systems
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- A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
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- siRNA stabilization prolongs gene knockdown in primary T lymphocytes
- (2008) Andrej Mantei et al. EUROPEAN JOURNAL OF IMMUNOLOGY
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