Current progress and challenges in crop genetic transformation

Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene

(2020) Raviraj Banakar et al.   Rice

Hairy Root Cultures—A Versatile Tool With Multiple Applications

(2020) Noemi Gutierrez-Valdes et al.   Frontiers in Plant Science

Gene transfer to plants by electroporation: methods and applications

(2020) Ibrahim Ilker Ozyigit   MOLECULAR BIOLOGY REPORTS

Enhancing African orphan crops with genomics

(2020) Ramni Jamnadass et al.   NATURE GENETICS

Gene expression variability between randomly and targeted transgene integration events in tobacco suspension cell lines

(2020) Janina Kirchhoff et al.   Plant Biotechnology Reports

Genetically modified crops: current status and future prospects

(2020) Krishan Kumar et al.   PLANTA

Robust and Reproducible Agrobacterium-Mediated Transformation System of the C4 Genetic Model Species Setaria viridis

(2020) Duc Quan Nguyen et al.   Frontiers in Plant Science

Complex Trait Loci in Maize Enabled by CRISPR-Cas9 Mediated Gene Insertion

(2020) Huirong Gao et al.   Frontiers in Plant Science

Insights Into Genetic and Molecular Elements for Transgenic Crop Development

(2020) Marcos Fernando Basso et al.   Frontiers in Plant Science

Soybean Embryonic Axis Transformation: Combining Biolistic and Agrobacterium-Mediated Protocols to Overcome Typical Complications of In Vitro Plant Regeneration

(2020) Bruno Paes de Melo et al.   Frontiers in Plant Science

A GRF–GIF chimeric protein improves the regeneration efficiency of transgenic plants

(2020) Juan M. Debernardi et al.   NATURE BIOTECHNOLOGY

Nanoparticle‐mediated gene transformation strategies for plant genetic engineering

(2020) Zongyou Lv et al.   PLANT JOURNAL

Multiplying the efficiency and impact of biofortification through metabolic engineering

(2020) Dominique Van Der Straeten et al.   Nature Communications

Overexpression of the Transcription Factor GROWTH-REGULATING FACTOR5 Improves Transformation of Dicot and Monocot Species

(2020) Jixiang Kong et al.   Frontiers in Plant Science

Potential, Challenges, and Threats for the Application of New Breeding Techniques by the Private Plant Breeding Sector in the EU

(2020) Petra Jorasch   Frontiers in Plant Science

Direct Germline Transformation of Cotton Meristem Explants With No Selection

(2020) Yurong Chen et al.   Frontiers in Plant Science

Agrobacterium strains and strain improvement: Present and outlook

(2020) Jonas De Saeger et al.   BIOTECHNOLOGY ADVANCES

The barley pan-genome reveals the hidden legacy of mutation breeding

(2020) Murukarthick Jayakodi et al.   NATURE

Multiple wheat genomes reveal global variation in modern breeding

(2020) Sean Walkowiak et al.   NATURE

The CRISPR/Cas9 system and its applications in crop genome editing

(2019) Aili Bao et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Edit at Will: Genotype Independent Plant Transformation in the Era of Advanced Genomics and Genome Editing

(2019) Albert P. Kausch et al.   PLANT SCIENCE

Regeneration of Solanum tuberosum plants from protoplasts induces widespread genome instability

(2019) Michelle Fossi et al.   PLANT PHYSIOLOGY

Generation of a selectable marker free, highly expressed single copy locus as landing pad for transgene stacking in sugarcane

(2019) Yang Zhao et al.   PLANT MOLECULAR BIOLOGY

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

(2019) Claudia Garcia et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Improved plant regeneration in callus cultures of Sorghum bicolor (L.) Moench

(2019) Mariola Dreger et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Construction of Marker-Free Genetically Modified Maize Using a Heat-Inducible Auto-Excision Vector

(2019) Dengxiang Du et al.   Genes

Matrix attachment regions as a tool to influence plant transgene expression

(2019) Anna Sergeevna Dolgova et al.   3 Biotech

Breeding crops to feed 10 billion

(2019) Lee T. Hickey et al.   NATURE BIOTECHNOLOGY

How nanocarriers delivering cargos in plants can change the GMO landscape

(2019) Markita P. Landry et al.   Nature Nanotechnology

Orphan crops: their importance and the urgency of improvement

(2019) Zerihun Tadele   PLANTA

Haplotype-resolved genomes of geminivirus-resistant and geminivirus-susceptible African cassava cultivars

(2019) Joel-E. Kuon et al.   BMC BIOLOGY

Blurring the boundaries between cereal crops and model plants

(2019) Philippa Borrill   NEW PHYTOLOGIST

Somatic Embryogenesis in the Family Gentianaceae and Its Biotechnological Application

(2019) Karolina Tomiczak et al.   Frontiers in Plant Science

The Future of Digital Sequence Information for Plant Genetic Resources for Food and Agriculture

(2019) Sylvain Aubry   Frontiers in Plant Science

Lipofection-mediated genome editing using DNA-free delivery of the Cas9/gRNA ribonucleoprotein into plant cells

(2019) Wusheng Liu et al.   PLANT CELL REPORTS

Super-Pangenome by Integrating the Wild Side of a Species for Accelerated Crop Improvement

(2019) Aamir W. Khan et al.   TRENDS IN PLANT SCIENCE

Assembly of chromosome-scale contigs by efficiently resolving repetitive sequences with long reads

(2019) Huilong Du et al.   Nature Communications

Super-Agrobacterium ver. 4: Improving the Transformation Frequencies and Genetic Engineering Possibilities for Crop Plants

(2019) Satoko Nonaka et al.   Frontiers in Plant Science

A Fruitful Decade Using Synthetic Promoters in the Improvement of Transgenic Plants

(2019) Sajid Ali et al.   Frontiers in Plant Science

An improved Agrobacterium-mediated transformation method for cotton (Gossypium hirsutum L. ‘KC3’) assisted by microinjection and sonication

(2019) P. Gurusaravanan et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Plant gene editing through de novo induction of meristems

(2019) Michael F. Maher et al.   NATURE BIOTECHNOLOGY

CRISPR/Cas System: Recent Advances and Future Prospects for Genome Editing

(2019) Hakim Manghwar et al.   TRENDS IN PLANT SCIENCE

Rapid genotype “independent” Zea mays L. (maize) transformation via direct somatic embryogenesis

(2018) Keith Lowe et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of the Waxy gene in elite rice varieties

(2018) Jinshan Zhang et al.   Journal of Integrative Plant Biology

A multi-landing pad DNA integration platform for mammalian cell engineering

(2018) Leonid Gaidukov et al.   NUCLEIC ACIDS RESEARCH

Embryo-specific expression of a visual reporter gene as a selection system for citrus transformation

(2018) Manjul Dutt et al.   PLoS One

The assessment of field trials in GMO research around the world and their possible integration in field trials for variety registration

(2018) M. M. Slot et al.   TRANSGENIC RESEARCH

A guide to sequence your favorite plant genomes

(2018) Fay-Wei Li et al.   Applications in Plant Sciences

Genotype-independent and enhanced in planta Agrobacterium tumefaciens-mediated genetic transformation of peanut [Arachis hypogaea (L.)]

(2018) Sivabalan Karthik et al.   3 Biotech

Genotype-independent and enhanced in planta Agrobacterium tumefaciens-mediated genetic transformation of peanut [Arachis hypogaea (L.)]

(2018) Sivabalan Karthik et al.   3 Biotech

Priorities for science to overcome hurdles thwarting the full promise of the “digital agriculture” revolution

(2018) Mark Shepherd et al.   JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE

Chromosome assembly of large and complex genomes using multiple references

(2018) Mikhail Kolmogorov et al.   GENOME RESEARCH

CRISPR–Cas9-mediated genome editing in apple and grapevine

(2018) Yuriko Osakabe et al.   Nature Protocols

Explant type and stress treatment determine the uni- and multicellular origin of somatic embryos in the tree fern Cyathea delgadii Sternb.

(2018) Małgorzata Grzyb et al.   PLANT CELL TISSUE AND ORGAN CULTURE

The DFR locus: A smart landing pad for targeted transgene insertion in tomato

(2018) Benoit Danilo et al.   PLoS One

Stable pH Suppresses Defense Signaling and is the Key to Enhance Agrobacterium-Mediated Transient Expression in Arabidopsis Seedlings

(2018) Yi-Chieh Wang et al.   Scientific Reports

A Highly Characterized Synthetic Landing Pad System for Precise Multicopy Gene Integration in Yeast

(2018) Leanne Bourgeois et al.   ACS Synthetic Biology

Current Strategies of Polyploid Plant Genome Sequence Assembly

(2018) Maria Kyriakidou et al.   Frontiers in Plant Science

DNA-Free Genome Editing of Brassica oleracea and B. rapa Protoplasts Using CRISPR-Cas9 Ribonucleoprotein Complexes

(2018) Jana Murovec et al.   Frontiers in Plant Science

Environmental impacts of genetically modified plants: A review

(2017) Aristidis M. Tsatsakis et al.   ENVIRONMENTAL RESEARCH

A chromosome conformation capture ordered sequence of the barley genome

(2017) Martin Mascher et al.   NATURE

Generation of genetically stable transformants by Agrobacterium using tomato floral buds

(2017) Miduthuri Satya Sharada et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Advances in Maize Transformation Technologies and Development of Transgenic Maize

(2017) Pranjal Yadava et al.   Frontiers in Plant Science

In planta Agrobacterium -Mediated Transformation of Rice

(2017) Kumrop Ratanasut et al.   Rice Science

Single genetic locus improvement of iron, zinc and β-carotene content in rice grains

(2017) Simrat Pal Singh et al.   Scientific Reports

The socioeconomics of genetically modified biofortified crops: a systematic review and meta-analysis

(2016) Hans De Steur et al.   Annals of the New York Academy of Sciences

Global Adoption of Genetically Modified (GM) Crops: Challenges for the Public Sector

(2016) Joseph E. Huesing et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology

(2016) Jeyabharathy Chandrasekaran et al.   MOLECULAR PLANT PATHOLOGY

Variation in the flowering gene SELF PRUNING 5G promotes day-neutrality and early yield in tomato

(2016) Sebastian Soyk et al.   NATURE GENETICS

ARGOS8 variants generated by CRISPR-Cas9 improve maize grain yield under field drought stress conditions

(2016) Jinrui Shi et al.   PLANT BIOTECHNOLOGY JOURNAL

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

(2016) Keith Lowe et al.   PLANT CELL

Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts

(2016) Mariette Andersson et al.   PLANT CELL REPORTS

Spike-dip transformation ofSetaria viridis

(2016) Prasenjit Saha et al.   PLANT JOURNAL

An efficient approach to BAC based assembly of complex genomes

(2016) Paul Visendi et al.   Plant Methods

Opportunities for Products of New Plant Breeding Techniques

(2016) Jan G. Schaart et al.   TRENDS IN PLANT SCIENCE

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

(2016) Sergei Svitashev et al.   Nature Communications

Application of Somatic Embryogenesis in Woody Plants.

(2016) Yuan Guan et al.   Frontiers in Plant Science

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

(2015) Je Wook Woo et al.   NATURE BIOTECHNOLOGY

DNA library construction using Gibson Assembly®

(2015) Steven Thomas et al.   NATURE METHODS

Standards for plant synthetic biology: a common syntax for exchange of DNA parts

(2015) Nicola J. Patron et al.   NEW PHYTOLOGIST

Transgenic trait deployment using designed nucleases

(2015) Joseph F. Petolino et al.   PLANT BIOTECHNOLOGY JOURNAL

The impact of secondary pests onBacillus thuringiensis (Bt)crops

(2015) Rui Catarino et al.   PLANT BIOTECHNOLOGY JOURNAL

Synthetic promoters in planta

(2015) Nrisingha Dey et al.   PLANTA

A transgenic, visual screenable marker for soybean seeds

(2015) Lisa Kanizay et al.   TRANSGENIC RESEARCH

Public Acceptance of Plant Biotechnology and GM Crops

(2015) Jan Lucht   Viruses-Basel

Transition from somatic embryo to friable embryogenic callus in cassava: dynamic changes in cellular structure, physiological status, and gene expression profiles

(2015) Qiuxiang Ma et al.   Frontiers in Plant Science

Genome-wide analysis of local chromatin packing inArabidopsis thaliana

(2014) Congmao Wang et al.   GENOME RESEARCH

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

(2014) Yanpeng Wang et al.   NATURE BIOTECHNOLOGY

Insights into the Maize Pan-Genome and Pan-Transcriptome

(2014) C. N. Hirsch et al.   PLANT CELL

Plant Transformation via Pollen Tube-Mediated Gene Transfer

(2014) Asjad Ali et al.   PLANT MOLECULAR BIOLOGY REPORTER

A Modular Plasmid Assembly Kit for Multigene Expression, Gene Silencing and Silencing Rescue in Plants

(2014) Andreas Binder et al.   PLoS One

A Meta-Analysis of the Impacts of Genetically Modified Crops

(2014) Wilhelm Klümper et al.   PLoS One

A meta-analysis of crop yield under climate change and adaptation

(2014) A. J. Challinor et al.   Nature Climate Change

Agrobacterium-mediated genetic transformation of yam (Dioscorea rotundata): an important tool for functional study of genes and crop improvement

(2014) Evans Nyaboga et al.   Frontiers in Plant Science

A dubious success: The NGO campaign against GMOs

(2014) Robert Paarlberg   GM Crops & Food-Biotechnology in Agriculture and the Food Chain

Assessment of Factors Influencing the Agrobacterium-mediated in planta Seed Transformation of Brinjal (Solanum melongena L.)

(2013) Kondeti Subramanyam et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Less is more: strategies to remove marker genes from transgenic plants

(2013) Yuan-Yeu Yau et al.   BMC BIOTECHNOLOGY

Identification and validation of promoters and cis-acting regulatory elements

(2013) Carlos M. Hernandez-Garcia et al.   PLANT SCIENCE

Yield Trends Are Insufficient to Double Global Crop Production by 2050

(2013) Deepak K. Ray et al.   PLoS One

GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis

(2013) Athanasios Lampropoulos et al.   PLoS One

Towards revealing the functions of all genes in plants

(2013) Seung Yon Rhee et al.   TRENDS IN PLANT SCIENCE

Unlocking the potential of tropical root crop biotechnology in east Africa by establishing a genetic transformation platform for local farmer-preferred cassava cultivars

(2013) Evans Nyaboga et al.   Frontiers in Plant Science

Agrobacterium infection and plant defense—transformation success hangs by a thread

(2013) Andrea Pitzschke   Frontiers in Plant Science

Selectable Markers and Reporter Genes: A Well Furnished Toolbox for Plant Science and Genetic Engineering

(2012) Daniele Rosellini   CRITICAL REVIEWS IN PLANT SCIENCES

Development of an Agricultural Biotechnology Crop Product: Testing from Discovery to Commercialization

(2012) Laura S. Privalle et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

The banana (Musa acuminata) genome and the evolution of monocotyledonous plants

(2012) Angélique D’Hont et al.   NATURE

The tomato genome sequence provides insights into fleshy fruit evolution

(2012)   NATURE

Empowering biotechnology in southern Africa: establishment of a robust transformation platform for the production of transgenic industry-preferred cassava

(2012) C.C. Chetty et al.   New Biotechnology

The elimination of a selectable marker gene in the doubled haploid progeny of co-transformed barley plants

(2012) Eszter Kapusi et al.   PLANT MOLECULAR BIOLOGY

Enhanced Agrobacterium-mediated transformation efficiencies in monocot cells is associated with attenuated defense responses

(2012) Wan-Jun Zhang et al.   PLANT MOLECULAR BIOLOGY

Green Revolution: Impacts, limits, and the path ahead

(2012) P. L. Pingali   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Monitoring transgene expression levels in different genotypes of field grown maize (Zea mays L.)

(2012) A. Bakó et al.   SOUTH AFRICAN JOURNAL OF BOTANY

Recent patterns of crop yield growth and stagnation

(2012) Deepak K. Ray et al.   Nature Communications

A non-tissue culture approach for developing transgenic Brassica juncea L. plants with Agrobacterium tumefaciens

(2011) Sudesh Chhikara et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Genome sequence and analysis of the tuber crop potato

(2011) Xun Xu et al.   NATURE

Optimization of Brassica napus (canola) explant regeneration for genetic transformation

(2011) Priti Maheshwari et al.   New Biotechnology

Cassava: constraints to production and the transfer of biotechnology to African laboratories

(2011) Simon E. Bull et al.   PLANT CELL REPORTS

A Modular Cloning System for Standardized Assembly of Multigene Constructs

(2011) Ernst Weber et al.   PLoS One

GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules

(2011) Alejandro Sarrion-Perdigones et al.   PLoS One

Climate Trends and Global Crop Production Since 1980

(2011) D. B. Lobell et al.   SCIENCE

Transgenic soybean with low phytate content constructed by Agrobacterium transformation and pollen-tube pathway

(2010) Shaohui Yang et al.   EUPHYTICA

Transgene Expression and Transgene-Induced Silencing in Diploid and Autotetraploid Arabidopsis

(2010) T. E. Finn et al.   GENETICS

GM Risk Assessment

(2010) P. A. C. Sparrow   MOLECULAR BIOTECHNOLOGY

Benefits of genetically modified crops for the poor: household income, nutrition, and health

(2010) Matin Qaim   New Biotechnology

The Sorghum bicolor genome and the diversification of grasses

(2009) Andrew H. Paterson et al.   NATURE

Precise genome modification in the crop species Zea mays using zinc-finger nucleases

(2009) Vipula K. Shukla et al.   NATURE

Agrobacterium-mediated transformation of friable embryogenic calli and regeneration of transgenic cassava

(2009) S E Bull et al.   Nature Protocols

Evidence for stable transformation of wheat by floral dip in Agrobacterium tumefaciens

(2009) Janice M. Zale et al.   PLANT CELL REPORTS

Effects of type of explant and age, plant growth regulators and medium strength on somatic embryogenesis and plant regeneration in Eucalyptus camaldulensis

(2009) M. G. Prakash et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Agrobacterium in the Genomics Age

(2009) S. B. Gelvin   PLANT PHYSIOLOGY

The B73 Maize Genome: Complexity, Diversity, and Dynamics

(2009) P. S. Schnable et al.   SCIENCE

1-Aminocyclopropane-1-Carboxylate Deaminase Enhances Agrobacterium tumefaciens-Mediated Gene Transfer into Plant Cells

(2008) S. Nonaka et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Detection of vector- and selectable marker-free transgenic maize with a linear GFP cassette transformation via the pollen-tube pathway

(2008) Aifu Yang et al.   JOURNAL OF BIOTECHNOLOGY

Advances in selectable marker genes for plant transformation

(2008) Isaac Kirubakaran Sundar et al.   JOURNAL OF PLANT PHYSIOLOGY

Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed

(2008) Yukoh Hiei et al.   Nature Protocols

Ethylene production in plants during transformation suppressesvirgene expression inAgrobacterium tumefaciens

(2008) Satoko Nonaka et al.   NEW PHYTOLOGIST

Evaluation of a morphological marker selection and excision system to generate marker-free transgenic cassava plants

(2008) Laddawan Saelim et al.   PLANT CELL REPORTS

In Planta Transformation of Tomato

(2008) Abida Yasmeen et al.   PLANT MOLECULAR BIOLOGY REPORTER