Selectable Markers and Reporter Genes: A Well Furnished Toolbox for Plant Science and Genetic Engineering

Gabaculine selection using bacterial and plant marker genes (GSA-AT) in durum wheat transformation

(2012) Angelica Giancaspro et al.   PLANT CELL TISSUE AND ORGAN CULTURE

In vivo visualization of RNA in plants cells using the λN22 system and a GATEWAY-compatible vector series for candidate RNAs

(2012) Johannes Schönberger et al.   PLANT JOURNAL

Selectable marker genes from plants: reliability and potential

(2011) Daniele Rosellini   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Evaluation of the E. coli d-serine ammonia lyase gene (Ec. dsdA) for use as a selectable marker in maize transformation

(2011) Fang-Ming Lai et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

A point mutation in the Medicago sativa GSA gene provides a novel, efficient, selectable marker for plant genetic engineering

(2011) N. Ferradini et al.   JOURNAL OF BIOTECHNOLOGY

pHairyRed: A Novel Binary Vector Containing the DsRed2 Reporter Gene for Visual Selection of Transgenic Hairy Roots

(2011) Meng-Han Lin et al.   Molecular Plant

Glow in the Dark: Fluorescent Proteins as Cell and Tissue-Specific Markers in Plants

(2011) Wenzislava Ckurshumova et al.   Molecular Plant

A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching

(2011) Tanja Brakemann et al.   NATURE BIOTECHNOLOGY

High-throughput screening for plant defense activators using a .BETA.-glucuronidase-reporter gene assay in Arabidopsis thaliana

(2011) Yoshihiro Narusaka et al.   Plant Biotechnology

A selection system for transgenic Arabidopsis thaliana using potassium thiocyanate as the selective agent and AtHOL1 as the selective marker

(2011) Keiko Midorikawa et al.   Plant Biotechnology

Transformation of Arabidopsis by mutated acetolactate synthase genes from rice and Arabidopsis that confer specific resistance to pyrimidinylcarboxylate-type ALS inhibitors

(2011) Kiyoshi Kawai et al.   Plant Biotechnology

Cre-loxP mediated marker elimination and gene reactivation at the waxy locus created in rice genome based on strong positive-negative selection

(2011) Rie Terada et al.   Plant Biotechnology

The chloroplast transformation toolbox: selectable markers and marker removal

(2011) Anil Day et al.   PLANT BIOTECHNOLOGY JOURNAL

Improved and high throughput quantitative measurements of weak GFP expression in transgenic plant materials

(2011) Jing-Jing Wu et al.   PLANT CELL REPORTS

Variation in GUS activity in vegetatively propagated Hevea brasiliensis transgenic plants

(2011) Ludovic Lardet et al.   PLANT CELL REPORTS

Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor

(2011) Iris Heidmann et al.   PLANT CELL REPORTS

A fluorescent reporter protein containing AtRMR1 domains is targeted to the storage and central vacuoles in Arabidopsis thaliana and tobacco leaf cells

(2011) Camila María Scabone et al.   PLANT CELL REPORTS

Assessment of simple marker-free genetic transformation techniques in alfalfa

(2011) Nicoletta Ferradini et al.   PLANT CELL REPORTS

Loss of AtPDR11, a plasma membrane-localized ABC transporter, confers paraquat tolerance in Arabidopsis thaliana

(2011) Jing Xi et al.   PLANT JOURNAL

FRET-based genetically encoded sensors allow high-resolution live cell imaging of Ca2+ dynamics

(2011) Melanie Krebs et al.   PLANT JOURNAL

pH-sensitivity of YFP provides an intracellular indicator of programmed cell death

(2011) Bennett Young et al.   Plant Methods

Plastid biotechnology for crop production: present status and future perspectives

(2011) Jihong Liu Clarke et al.   PLANT MOLECULAR BIOLOGY

Coupling Virus-Induced Gene Silencing to Exogenous Green Fluorescence Protein Expression Provides a Highly Efficient System for Functional Genomics in Arabidopsis and across All Stages of Tomato Fruit Development

(2011) L. Quadrana et al.   PLANT PHYSIOLOGY

Color Recovery after Photoconversion of H2B::mEosFP Allows Detection of Increased Nuclear DNA Content in Developing Plant Cells

(2011) M. Wozny et al.   PLANT PHYSIOLOGY

Application of Gene Targeting to Designed Mutation Breeding of High-Tryptophan Rice

(2011) H. Saika et al.   PLANT PHYSIOLOGY

Evaluation of Genetically Engineered Crops Using Transcriptomic, Proteomic, and Metabolomic Profiling Techniques

(2011) A. E. Ricroch et al.   PLANT PHYSIOLOGY

Arabidopsis ENHANCER OF SHOOT REGENERATION (ESR)1 and ESR2 regulate in vitro shoot regeneration and their expressions are differentially regulated

(2011) Naoki Matsuo et al.   PLANT SCIENCE

Visualisation of stromules in transgenic wheat expressing a plastid-targeted yellow fluorescent protein

(2011) Daniel J. Shaw et al.   PLANTA

Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish

(2011) A. Muto et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An Expanded Palette of Genetically Encoded Ca2+ Indicators

(2011) Y. Zhao et al.   SCIENCE

Use of the VvMybA1 gene for non-destructive quantification of promoter activity via color histogram analysis in grapevine (Vitis vinifera) and tobacco

(2011) Zhijian T. Li et al.   TRANSGENIC RESEARCH

Anthocyanin production as a potential visual selection marker during plant transformation

(2011) A. J. Kortstee et al.   TRANSGENIC RESEARCH

New biotech applications from evolved D-amino acid oxidases

(2011) Loredano Pollegioni et al.   TRENDS IN BIOTECHNOLOGY

Over-expression of the Arabidopsis Na+/H+ antiporter gene in Populus deltoides CL × P. euramericana CL “NL895” enhances its salt tolerance

(2010) Guirong Qiao et al.   ACTA PHYSIOLOGIAE PLANTARUM

DIANTHIN, a negative selection marker in tobacco, is non-toxic in transgenic rice and confers sheath blight resistance

(2010) J. M. Shah et al.   BIOLOGIA PLANTARUM

High level transgenic expression of soybean (Glycine max) GmERF and Gmubi gene promoters isolated by a novel promoter analysis pipeline

(2010) Carlos M Hernandez-Garcia et al.   BMC PLANT BIOLOGY

Expression of green fluorescent protein inCurvularia lunatacausing maize leaf spot

(2010) Tong Liu et al.   CANADIAN JOURNAL OF PLANT PATHOLOGY

Bifunctional selection–reporter systems for genetic transformation of citrus: mannose- and kanamycin-based systems

(2010) Manjul Dutt et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Site-specific gene integration technologies for crop improvement

(2010) Vibha Srivastava et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Evaluation of green fluorescent protein as a reporter gene and phosphinothricin as the selective agent for achieving a higher recovery of transformants in cucumber (Cucumis sativus L. cv. Poinsett76) via Agrobacterium tumefaciens

(2010) N. Selvaraj et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Alanine racemase as a novel positive selectable marker for plant transformation

(2010) Venkatesan Thiruvengadam et al.   JOURNAL OF BIOTECHNOLOGY

The application of the yeast N-acetyltransferase MPR1 gene and the proline analogue L-azetidine-2-carboxylic acid as a selectable marker system for plant transformation

(2010) F.-Y. Tsai et al.   JOURNAL OF EXPERIMENTAL BOTANY

Novel Insights from Live-imaging in Shoot Meristem Development

(2010) Paja Sijacic et al.   Journal of Integrative Plant Biology

Combination of Fluorescent Reporters for Simultaneous Monitoring of Root Colonization and Antifungal Gene Expression by a Biocontrol Pseudomonad on Cereals with Flow Cytometry

(2010) Laurène Rochat et al.   MOLECULAR PLANT-MICROBE INTERACTIONS

A novel light-dependent selection marker system in plants

(2010) Serry Koh et al.   PLANT BIOTECHNOLOGY JOURNAL

Evaluation and application of a luciferase fusion system for rapidin vivoanalysis of RNAi targets and constructs in plants

(2010) Robert G. Birch et al.   PLANT BIOTECHNOLOGY JOURNAL

d-glufosinate as a male sterility agent for hybrid seed production

(2010) Tim Hawkes et al.   PLANT BIOTECHNOLOGY JOURNAL

Production of marker-free disease-resistant potato using isopentenyl transferase gene as a positive selection marker

(2010) Raham Sher Khan et al.   PLANT CELL REPORTS

Selectable marker elimination in the T0 generation by Agrobacterium-mediated co-transformation involving Mungbean yellow mosaic virus TrAP as a non-conditional negative selectable marker and bar for transient positive selection

(2010) Mangu Venkata RamanaRao et al.   PLANT CELL REPORTS

An efficient method for the production of marker-free transgenic plants of peanut (Arachis hypogaea L.)

(2010) Madhurima Bhatnagar et al.   PLANT CELL REPORTS

Transgenic hybrid aspen overexpressing the Atwbc19 gene encoding an ATP-binding cassette transporter confers resistance to four aminoglycoside antibiotics

(2010) Byung-guk Kang et al.   PLANT CELL REPORTS

The green fluorescent protein as an efficient selection marker for Agrobacterium tumefaciens-mediated transformation in Hevea brasiliensis (Müll. Arg)

(2010) J. Leclercq et al.   PLANT CELL REPORTS

Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols

(2010) I. M. G. Padilla et al.   PLANT CELL REPORTS

Selecting genetic transformants of indica and indica-derived rice cultivars using bispyribac sodium and a mutated ALS gene

(2010) Yojiro Taniguchi et al.   PLANT CELL REPORTS

Estrogen-inducible GFP expression patterns in rice (Oryza sativa L.)

(2010) Ayako Okuzaki et al.   PLANT CELL REPORTS

Recombinase technology: applications and possibilities

(2010) Yueju Wang et al.   PLANT CELL REPORTS

Chloroplast transformation of rapeseed (Brassica napus) by particle bombardment of cotyledons

(2010) Lin Cheng et al.   PLANT CELL REPORTS

Isopentenyl transferase gene expression offers the positive selection of marker-free transgenic plant of Kalanchoe blossfeldiana

(2010) Gunaratnam Thirukkumaran et al.   PLANT CELL TISSUE AND ORGAN CULTURE

PMI (manA) as a nonantibiotic selectable marker gene in plant biotechnology

(2010) P. Stoykova et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Phosphomannose-isomerase as a selectable marker to recover transgenic orchid plants (Oncidium Gower Ramsey)

(2010) Muthu Thiruvengadam et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Rol (root loci) gene as a positive selection marker to produce marker-free Petunia hybrida

(2010) Raham Sher Khan et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Transformation of Liquidambar formosana L. via Agrobacterium tumefaciens using a mannose selection system and recovery of salt tolerant lines

(2010) Guirong Qiao et al.   PLANT CELL TISSUE AND ORGAN CULTURE

High throughput generation of promoter reporter (GFP) transgenic lines of low expressing genes in Arabidopsis and analysis of their expression patterns

(2010) Yong-Li Xiao et al.   Plant Methods

The effect of different 3′ untranslated regions on the accumulation and stability of transcripts of a gfp transgene in chloroplasts of transplastomic tobacco

(2010) Sithichoke Tangphatsornruang et al.   PLANT MOLECULAR BIOLOGY

Visual spectinomycin resistance (aadA au ) gene for facile identification of transplastomic sectors in tobacco leaves

(2010) Tarinee Tungsuchat-Huang et al.   PLANT MOLECULAR BIOLOGY

Chloramphenicol acetyltransferase as selectable marker for plastid transformation

(2010) Weimin Li et al.   PLANT MOLECULAR BIOLOGY

mEosFP-Based Green-to-Red Photoconvertible Subcellular Probes for Plants

(2010) J. Mathur et al.   PLANT PHYSIOLOGY

Nontransgenic Genome Modification in Plant Cells

(2010) I. Marton et al.   PLANT PHYSIOLOGY

Exploring multiple drug and herbicide resistance in plants—Spotlight on transporter proteins

(2010) Sarah S. Conte et al.   PLANT SCIENCE

Over-expression of peptide deformylase in chloroplasts confers actinonin resistance, but is not a suitable selective marker system for plastid transformation

(2010) Alicia Fernández-San Millán et al.   TRANSGENIC RESEARCH

A novel strategy for obtaining kanamycin resistance in Arabidopsis thaliana by silencing an endogenous gene encoding a putative chloroplast transporter

(2009) Werner Aufsatz et al.   Biotechnology Journal

Glyphosate Resistance by Engineering the Flavoenzyme Glycine Oxidase

(2009) Mattia Pedotti et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Tobacco plastid transformation using the feedback-insensitive anthranilate synthase [ ]-subunit of tobacco (ASA2) as a new selectable marker

(2009) P. Barone et al.   JOURNAL OF EXPERIMENTAL BOTANY

Distinguishing transgenic from non-transgenic Arabidopsis plants by 1H NMR-based metabolic fingerprinting

(2009) Yanfei Ren et al.   Journal of Genetics and Genomics

A comparative proteomics approach to detect unintended effects in transgenic Arabidopsis

(2009) Yanfei Ren et al.   Journal of Genetics and Genomics

High-frequency modification of plant genes using engineered zinc-finger nucleases

(2009) Jeffrey A. Townsend et al.   NATURE

Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators

(2009) Lin Tian et al.   NATURE METHODS

The pleiotropic effects of thebargene and glufosinate on theArabidopsistranscriptome

(2009) Ashraf Abdeen et al.   PLANT BIOTECHNOLOGY JOURNAL

Selectable marker genes and unintended changes to the plant transcriptome

(2009) Brian Miki et al.   PLANT BIOTECHNOLOGY JOURNAL

Tryptophan synthesis block-associated sugar response, a physiological marker for rapid selection ofArabidopsis thalianatransformants, marked with feedback-inhibition-insensitive anthranilate synthase gene

(2009) T. Nonomura et al.   PLANT BREEDING

Combining a regeneration-promoting ipt gene and site-specific recombination allows a more efficient apricot transformation and the elimination of marker genes

(2009) Sonia López-Noguera et al.   PLANT CELL REPORTS

Expression of a glycosylated GFP as a bivalent reporter in exocytosis

(2009) Nadine Paris et al.   PLANT CELL REPORTS

A novel mannose-based selection system for plant transformation using celery mannose-6-phosphate reductase gene

(2009) Guo-qing Song et al.   PLANT CELL REPORTS

Development of a phosphomannose isomerase-based Agrobacterium-mediated transformation system for chickpea (Cicer arietinum L.)

(2009) Gunvant Patil et al.   PLANT CELL REPORTS

The application of the mutated acetolactate synthase gene from rice as the selectable marker gene in the production of transgenic soybeans

(2009) Makoto Tougou et al.   PLANT CELL REPORTS

Phosphomannose-isomerase (pmi) gene as a selectable marker for Agrobacterium-mediated transformation of rapeseed

(2009) M. Wallbraun et al.   PLANT CELL TISSUE AND ORGAN CULTURE

A rapid and non-destructive screenable marker, FAST, for identifying transformed seeds ofArabidopsis thaliana

(2009) Takashi L. Shimada et al.   PLANT JOURNAL

Impact of ubiquitous inhibitors on the GUS gene reporter system: evidence from the model plants Arabidopsis, tobacco and rice and correction methods for quantitative assays of transgenic and endogenous GUS

(2009) Simone Fior et al.   Plant Methods

Lysine racemase: a novel non-antibiotic selectable marker for plant transformation

(2009) I-Chieh Chen et al.   PLANT MOLECULAR BIOLOGY

A Visual Reporter System for Virus-Induced Gene Silencing in Tomato Fruit Based on Anthocyanin Accumulation

(2009) D. Orzaez et al.   PLANT PHYSIOLOGY

Multiple Antibiotic Resistance in Arabidopsis Is Conferred by Mutations in a Chloroplast-Localized Transport Protein

(2009) S. Conte et al.   PLANT PHYSIOLOGY

A novel method for induction of plant regeneration via somatic embryogenesis

(2009) Wei Deng et al.   PLANT SCIENCE

Plastid transformation in eggplant (Solanum melongena L.)

(2009) A. K. Singh et al.   TRANSGENIC RESEARCH

Use of phosphomannose isomerase-based selection system for Agrobacterium-mediated transformation of tomato and potato

(2008) J. Bříza et al.   BIOLOGIA PLANTARUM

A bacterial haloalkane dehalogenase (dhlA) gene as conditional negative selection marker for rice callus cells

(2008) Sarah K. Moore et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Advances in selectable marker genes for plant transformation

(2008) Isaac Kirubakaran Sundar et al.   JOURNAL OF PLANT PHYSIOLOGY

The salt-tolerance gene rstB can be used as a selectable marker in plant genetic transformation

(2008) Wan-Jun Zhang et al.   MOLECULAR BREEDING

Evaluation of a morphological marker selection and excision system to generate marker-free transgenic cassava plants

(2008) Laddawan Saelim et al.   PLANT CELL REPORTS

Evaluation of selection strategies alternative to nptII in genetic transformation of citrus

(2008) Alida Ballester et al.   PLANT CELL REPORTS

Production of transgenic barrel medic (Medicago truncatula Gaernt.) using the ipt-type MAT vector system and impairment of Recombinase-mediated excision events

(2008) L. Scaramelli et al.   PLANT CELL REPORTS

Efficient transformation of wheat by using a mutated rice acetolactate synthase gene as a selectable marker

(2008) Taiichi Ogawa et al.   PLANT CELL REPORTS

Live-cell imaging of viral RNA genomes using a Pumilio-based reporter

(2008) Jens Tilsner et al.   PLANT JOURNAL

Simultaneous excision of two transgene flanking sequences and resolution of complex integration loci

(2008) Sandeep Kumar et al.   PLANT MOLECULAR BIOLOGY

Selectable Tolerance to Herbicides by Mutated Acetolactate Synthase Genes Integrated into the Chloroplast Genome of Tobacco

(2008) M. Shimizu et al.   PLANT PHYSIOLOGY

Characterisation of a new reporter system allowing high throughput in planta screening for recombination events before and after controlled DNA double strand break induction

(2008) Sophie Wehrkamp-Richter et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Regeneration of transgenic rice plants using high salt for selection without the need for antibiotics or herbicides

(2008) Zhengge Zhu et al.   PLANT SCIENCE

A co-transformation system to produce transgenic grapevines free of marker genes

(2008) M. Dutt et al.   PLANT SCIENCE

A novel method for fluorometric continuous measurement of β-glucuronidase (GUS) activity using 4-methyl-umbelliferyl-β-d-glucuronide (MUG) as substrate

(2008) Simone Fior et al.   PLANT SCIENCE

Ovary-drip transformation: a simple method for directly generating vector- and marker-free transgenic maize (Zea mays L.) with a linear GFP cassette transformation

(2008) Aifu Yang et al.   PLANTA

Genetic transformation of the sugar beet plastome

(2008) Francesca De Marchis et al.   TRANSGENIC RESEARCH

Cytokinin vectors mediate marker-free and backbone-free plant transformation

(2008) Craig M. Richael et al.   TRANSGENIC RESEARCH

Development of transformation vectors based upon a modified plant α-tubulin gene as the selectable marker

(2007) A YEMETS et al.   CELL BIOLOGY INTERNATIONAL

Comparison of endoplasmic reticulum targeted and non-targeted cytoplasmic GFP as a selectable marker in citrus protoplast transformation

(2007) Ahmad A. Omar et al.   PLANT SCIENCE