The CRISPR/Cas9 system and its applications in crop genome editing

Harnessing CRISPR/Cas systems for programmable transcriptional and post-transcriptional regulation

(2018) Ahmed Mahas et al.   BIOTECHNOLOGY ADVANCES

Transforming plant biology and breeding with CRISPR/Cas9, Cas12 and Cas13

(2018) Patrick Schindele et al.   FEBS LETTERS

Utilization of a Genome-Edited Tomato (Solanum lycopersicum) with High Gamma Aminobutyric Acid Content in Hybrid Breeding

(2018) Jeongeun Lee et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of the Waxy gene in elite rice varieties

(2018) Jinshan Zhang et al.   Journal of Integrative Plant Biology

Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 in vitro transcripts or ribonucleoproteins

(2018) Zhen Liang et al.   Nature Protocols

Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus

(2018) Anca Macovei et al.   PLANT BIOTECHNOLOGY JOURNAL

Genome editing using CRISPR/Cas9–targeted mutagenesis: An opportunity for yield improvements of crop plants grown under environmental stresses

(2018) Mostafa Abdelrahman et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Genome engineering in ornamental plants: Current status and future prospects

(2018) Mitsuko Kishi-Kaboshi et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Genetic interaction involving photoperiod-responsive Hd1 promotes early flowering under long-day conditions in rice

(2018) Prasanta K. Subudhi et al.   Scientific Reports

A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants

(2018) Longzheng Chen et al.   Horticulture Research

CRISPR plants now subject to tough GM laws in European Union

(2018) Ewen Callaway   NATURE

Reduced Drought Tolerance by CRISPR/Cas9-Mediated SlMAPK3 Mutagenesis in Tomato Plants

(2017) Liu Wang et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

A simple and efficient method for CRISPR/Cas9-induced mutant screening

(2017) Yufeng Hua et al.   Journal of Genetics and Genomics

Temperature effect on CRISPR-Cas9 mediated genome editing

(2017) Guanghai Xiang et al.   Journal of Genetics and Genomics

CRISPR-P 2.0: An Improved CRISPR-Cas9 Tool for Genome Editing in Plants

(2017) Hao Liu et al.   Molecular Plant

CRISPR-GE: A Convenient Software Toolkit for CRISPR-Based Genome Editing

(2017) Xianrong Xie et al.   Molecular Plant

Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System

(2017) Yuming Lu et al.   Molecular Plant

Construction of a Genome-Wide Mutant Library in Rice Using CRISPR/Cas9

(2017) Xiangbing Meng et al.   Molecular Plant

Mapping the genomic landscape of CRISPR–Cas9 cleavage

(2017) Peter Cameron et al.   NATURE METHODS

CRISPR-S: an active interference element for a rapid and inexpensive selection of genome-edited, transgene-free rice plants

(2017) Hai-Ping Lu et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas9-mediated efficient targeted mutagenesis in grape in the first generation

(2017) Xianhang Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Multiplexed CRISPR/Cas9-mediated metabolic engineering of γ-aminobutyric acid levels in Solanum lycopersicum

(2017) Rui Li et al.   PLANT BIOTECHNOLOGY JOURNAL

High efficient multisites genome editing in allotetraploid cotton (Gossypium hirsutum ) using CRISPR/Cas9 system

(2017) Pengcheng Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker

(2017) Hongge Jia et al.   PLANT BIOTECHNOLOGY JOURNAL

RNA-guided Cas9 as an in vivo desired-target mutator in maize

(2017) Chuxi Li et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas9-mediated targeted mutagenesis of GmFT2a delays flowering time in soya bean

(2017) Yupeng Cai et al.   PLANT BIOTECHNOLOGY JOURNAL

Re-sequencing transgenic plants revealed rearrangements at T-DNA inserts, and integration of a short T-DNA fragment, but no increase of small mutations elsewhere

(2017) Henk J. Schouten et al.   PLANT CELL REPORTS

Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat

(2017) Yunwei Zhang et al.   PLANT JOURNAL

CRISPR-Cas9 Targeted Mutagenesis Leads to Simultaneous Modification of Different Homoeologous Gene Copies in Polyploid Oilseed Rape ( Brassica napus )

(2017) Janina Braatz et al.   PLANT PHYSIOLOGY

Generation of a Collection of Mutant Tomato Lines Using Pooled CRISPR Libraries

(2017) Thomas B. Jacobs et al.   PLANT PHYSIOLOGY

CRISPR-Cas9 based plant genome editing: Significance, opportunities and recent advances

(2017) Neelam Soda et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

(2017) Zhen Liang et al.   Nature Communications

Knockout of OsNramp5 using the CRISPR/Cas9 system produces low Cd-accumulating indica rice without compromising yield

(2017) Li Tang et al.   Scientific Reports

OsSAPK2 Confers Abscisic Acid Sensitivity and Tolerance to Drought Stress in Rice

(2017) Dengji Lou et al.   Frontiers in Plant Science

Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

(2017) Vladimir Nekrasov et al.   Scientific Reports

Efficient increase of ɣ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis

(2017) Satoko Nonaka et al.   Scientific Reports

The Challenges and Solutions for Cadmium-contaminated Rice in China: A Critical Review

(2016) Yuanan Hu et al.   ENVIRONMENT INTERNATIONAL

Engineering of CRISPR/Cas9-mediated potyvirus resistance in transgene-freeArabidopsisplants

(2016) Douglas E. Pyott et al.   MOLECULAR PLANT PATHOLOGY

Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology

(2016) Jeyabharathy Chandrasekaran et al.   MOLECULAR PLANT PATHOLOGY

Gene-edited CRISPR mushroom escapes US regulation

(2016) Emily Waltz   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

CRISPR-edited crops free to enter market, skip regulation

(2016) Emily Waltz   NATURE BIOTECHNOLOGY

Variation in the flowering gene SELF PRUNING 5G promotes day-neutrality and early yield in tomato

(2016) Sebastian Soyk et al.   NATURE GENETICS

Thecbfstriple mutants reveal the essential functions ofCBFsin cold acclimation and allow the definition of CBF regulons inArabidopsis

(2016) Yuxin Jia et al.   NEW PHYTOLOGIST

ARGOS8 variants generated by CRISPR-Cas9 improve maize grain yield under field drought stress conditions

(2016) Jinrui Shi et al.   PLANT BIOTECHNOLOGY JOURNAL

Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts

(2016) Mariette Andersson et al.   PLANT CELL REPORTS

Site-directed mutagenesis in Petunia × hybrida protoplast system using direct delivery of purified recombinant Cas9 ribonucleoproteins

(2016) Saminathan Subburaj et al.   PLANT CELL REPORTS

An Effective Strategy for Reliably Isolating Heritable and Cas9 -Free Arabidopsis Mutants Generated by CRISPR/Cas9-Mediated Genome Editing

(2016) Xiuhua Gao et al.   PLANT PHYSIOLOGY

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

(2016) Sergei Svitashev et al.   Nature Communications

Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

(2016) Yi Zhang et al.   Nature Communications

DNA-Free Genetically Edited Grapevine and Apple Protoplast Using CRISPR/Cas9 Ribonucleoproteins

(2016) Mickael Malnoy et al.   Frontiers in Plant Science

Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922

(2016) Fujun Wang et al.   PLoS One

Development of Commercial Thermo-sensitive Genic Male Sterile Rice Accelerates Hybrid Rice Breeding Using the CRISPR/Cas9-mediated TMS5 Editing System

(2016) Hai Zhou et al.   Scientific Reports

A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants

(2015) Xingliang Ma et al.   Molecular Plant

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

(2015) Je Wook Woo et al.   NATURE BIOTECHNOLOGY

High-throughput functional genomics using CRISPR–Cas9

(2015) Ophir Shalem et al.   NATURE REVIEWS GENETICS

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Comparison of T7E1 and Surveyor Mismatch Cleavage Assays to Detect Mutations Triggered by Engineered Nucleases

(2015) Léna Vouillot et al.   G3-Genes Genomes Genetics

How and why does tomato accumulate a large amount of GABA in the fruit?

(2015) Mariko Takayama et al.   Frontiers in Plant Science

The CRISPR–Cas system for plant genome editing: advances and opportunities

(2014) Vinay Kumar et al.   JOURNAL OF EXPERIMENTAL BOTANY

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

(2014) Yanpeng Wang et al.   NATURE BIOTECHNOLOGY

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

(2014) John G Doench et al.   NATURE BIOTECHNOLOGY

Genome editing in rice and wheat using the CRISPR/Cas system

(2014) Qiwei Shan et al.   Nature Protocols

The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation

(2014) Hui Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

Transcriptomic Analyses Indicate That Maize Ligule Development Recapitulates Gene Expression Patterns That Occur during Lateral Organ Initiation

(2014) Robyn Johnston et al.   PLANT CELL

Strigolactones and the control of plant development: lessons from shoot branching

(2014) Tanya Waldie et al.   PLANT JOURNAL

Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering inArabidopsis thaliana

(2014) Friedrich Fauser et al.   PLANT JOURNAL

High-Throughput Genome Editing and Phenotyping Facilitated by High Resolution Melting Curve Analysis

(2014) Holly R. Thomas et al.   PLoS One

Lateral organ boundaries 1 is a disease susceptibility gene for citrus bacterial canker disease

(2014) Y. Hu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Gene targeting using the Agrobacterium tumefaciens-mediated CRISPR-Cas system in rice

(2014) Rongfang Xu et al.   Rice

An Efficient Genotyping Method for Genome-modified Animals and Human Cells Generated with CRISPR/Cas9 System

(2014) Xiaoxiao Zhu et al.   Scientific Reports

A Bountiful Harvest: Genomic Insights into Crop Domestication Phenotypes

(2013) Kenneth M. Olsen et al.   Annual Review of Plant Biology

Genetic variation in four maturity genes affects photoperiod insensitivity and PHYA-regulated post-flowering responses of soybean

(2013) Meilan Xu et al.   BMC PLANT BIOLOGY

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

(2013) Jian-Feng Li et al.   NATURE BIOTECHNOLOGY

Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

(2013) Vladimir Nekrasov et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Targeted genome modification of crop plants using a CRISPR-Cas system

(2013) Qiwei Shan et al.   NATURE BIOTECHNOLOGY

Evolution of crop species: genetics of domestication and diversification

(2013) Rachel S. Meyer et al.   NATURE REVIEWS GENETICS

Construction of a genomewide RNAi mutant library in rice

(2013) Lei Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

(2013) Ophir Shalem et al.   SCIENCE

Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA

(2012) Hai Zhou et al.   CELL RESEARCH

Recent advances in development of marker-free transgenic plants: Regulation and biosafety concern

(2012) Narendra Tuteja et al.   JOURNAL OF BIOSCIENCES

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Cis-regulatory elements: molecular mechanisms and evolutionary processes underlying divergence

(2011) Patricia J. Wittkopp et al.   NATURE REVIEWS GENETICS

Evolution and classification of the CRISPR–Cas systems

(2011) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

A TALE nuclease architecture for efficient genome editing

(2010) Jeffrey C Miller et al.   NATURE BIOTECHNOLOGY

Site-directed mutagenesis in Arabidopsis using custom-designed zinc finger nucleases

(2010) K. Osakabe et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE