Less is more: strategies to remove marker genes from transgenic plants

The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis

(2012) James G Thomson et al.   BMC BIOTECHNOLOGY

High-efficiency TALEN-based gene editing produces disease-resistant rice

(2012) Ting Li et al.   NATURE BIOTECHNOLOGY

Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs

(2012) Lindsay Cade et al.   NUCLEIC ACIDS RESEARCH

TAL effectors are remote controls for gene activation

(2011) Heidi Scholze et al.   CURRENT OPINION IN MICROBIOLOGY

Knockout rats generated by embryo microinjection of TALENs

(2011) Laurent Tesson et al.   NATURE BIOTECHNOLOGY

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

(2011) Tomas Cermak et al.   NUCLEIC ACIDS RESEARCH

The chloroplast transformation toolbox: selectable markers and marker removal

(2011) Anil Day et al.   PLANT BIOTECHNOLOGY JOURNAL

Transgene stacking and marker elimination in transgenic rice by sequential Agrobacterium-mediated co-transformation with the same selectable marker gene

(2011) Mangu Venkata Ramana Rao et al.   PLANT CELL REPORTS

Transgene excision in pollen using a codon optimized serine resolvase CinH-RS2 site-specific recombination system

(2011) Hong S. Moon et al.   PLANT MOLECULAR BIOLOGY

A Method for Producing Transgenic Cells Using a Multi-Integrase System on a Human Artificial Chromosome Vector

(2011) Shigeyuki Yamaguchi et al.   PLoS One

Transgene introgression in crop relatives: molecular evidence and mitigation strategies

(2011) Charles Kwit et al.   TRENDS IN BIOTECHNOLOGY

Transformation System forHypocrea jecorina(Trichoderma reesei) That Favors Homologous Integration and Employs Reusable Bidirectionally Selectable Markers

(2010) Matthias G. Steiger et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

I-SceI endonuclease: a new tool for DNA repair studies and genetic manipulations in streptomycetes

(2010) Theresa Siegl et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome

(2010) James G Thomson et al.   BMC BIOTECHNOLOGY

Serine recombinases as tools for genome engineering

(2010) William R.A. Brown et al.   METHODS

TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain

(2010) Ting Li et al.   NUCLEIC ACIDS RESEARCH

Selectable marker elimination in the T0 generation by Agrobacterium-mediated co-transformation involving Mungbean yellow mosaic virus TrAP as a non-conditional negative selectable marker and bar for transient positive selection

(2010) Mangu Venkata RamanaRao et al.   PLANT CELL REPORTS

Zinc finger nuclease-mediated transgene deletion

(2010) Joseph F. Petolino et al.   PLANT MOLECULAR BIOLOGY

High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases

(2010) F. Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Site-specific DNA excision via engineered zinc finger nucleases

(2010) Hyung Joo Lee et al.   TRENDS IN BIOTECHNOLOGY

Genome editing in plant cells by zinc finger nucleases

(2010) Dan Weinthal et al.   TRENDS IN PLANT SCIENCE

PhiC31 integrase induces a DNA damage response and chromosomal rearrangements in human adult fibroblasts

(2009) Jian Liu et al.   BMC BIOTECHNOLOGY

Gene Targeting of a Disease-Related Gene in Human Induced Pluripotent Stem and Embryonic Stem Cells

(2009) Jizhong Zou et al.   Cell Stem Cell

Engineering DNA processing enzymes for the postgenomic era

(2009) Frank Buchholz   CURRENT OPINION IN BIOTECHNOLOGY

The humanitarian impact of plant biotechnology: recent breakthroughs vs bottlenecks for adoption

(2009) Gemma Farre et al.   CURRENT OPINION IN PLANT BIOLOGY

Targeted chromosomal deletions in human cells using zinc finger nucleases

(2009) H. J. Lee et al.   GENOME RESEARCH

High-frequency modification of plant genes using engineered zinc-finger nucleases

(2009) Jeffrey A. Townsend et al.   NATURE

Precise genome modification in the crop species Zea mays using zinc-finger nucleases

(2009) Vipula K. Shukla et al.   NATURE

Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases

(2009) Dirk Hockemeyer et al.   NATURE BIOTECHNOLOGY

Marker-free transgenic corn plant production through co-bombardment

(2009) N. Shiva Prakash et al.   PLANT CELL REPORTS

Evaluation of seven promoters to achieve germline directed Cre-lox recombination in Arabidopsis thaliana

(2009) Frédéric Van Ex et al.   PLANT CELL REPORTS

Targeted transgene integration in plant cells using designed zinc finger nucleases

(2009) Charles Q. Cai et al.   PLANT MOLECULAR BIOLOGY

Site-specific recombination for the modification of transgenic strains of the Mediterranean fruit fly Ceratitis capitata

(2009) M. F. Schetelig et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Generation of selectable marker-free transgenic tomato resistant to drought, cold and oxidative stress using the Cre/loxP DNA excision system

(2009) Yue Zhang et al.   TRANSGENIC RESEARCH

Auto-excision of selectable marker genes from transgenic tobacco via a stress inducible FLP/FRT site-specific recombination system

(2009) Hee-Jong Woo et al.   TRANSGENIC RESEARCH

Next-generation sequencing technologies and their implications for crop genetics and breeding

(2009) Rajeev K. Varshney et al.   TRENDS IN BIOTECHNOLOGY

Keeping the genie in the bottle: transgene biocontainment by excision in pollen

(2009) Hong S. Moon et al.   TRENDS IN BIOTECHNOLOGY

Production of selectable marker-free transgenic tobacco plants using a non-selection approach: chimerism or escape, transgene inheritance, and efficiency

(2008) Baochun Li et al.   PLANT CELL REPORTS

ParA resolvase catalyzes site-specific excision of DNA from the Arabidopsis genome

(2008) James G. Thomson et al.   TRANSGENIC RESEARCH