Plastid transformation: Advances and challenges for its implementation in agricultural crops

Transient expression of a green fluorescent protein in tobacco and maize chloroplast

(2020) Sigifredo Arévalo-Gallegos et al.   ELECTRONIC JOURNAL OF BIOTECHNOLOGY

Length-dependent accumulation of double-stranded RNAs in plastids affects RNA interference efficiency in the Colorado potato beetle

(2020) Wanwan He et al.   JOURNAL OF EXPERIMENTAL BOTANY

Protein Import Motors in Chloroplasts: On the Role of Chaperones

(2020) Hsou-min Li et al.   PLANT CELL

Cas9/gRNA-mediated genome editing of yeast mitochondria and Chlamydomonas chloroplasts

(2020) Byung-Chun Yoo et al.   PeerJ

Inaccessibility to double‐stranded RNAs in plastids restricts RNA interference in Bemisia tabaci (whitefly)

(2020) Yi Dong et al.   PEST MANAGEMENT SCIENCE

Chloroplast genome transformation of medicinal plant Artemisia annua

(2020) Charli Kaushal et al.   PLANT BIOTECHNOLOGY JOURNAL

Translocation from the chloroplast stroma into the thylakoid lumen allows expression of recombinant epidermal growth factor in transplastomic tobacco plants

(2020) Mauro M. Morgenfeld et al.   TRANSGENIC RESEARCH

Establishment of a chloroplast transformation system in Tisochrysis lutea

(2020) Yuntai Bo et al.   JOURNAL OF APPLIED PHYCOLOGY

A novel strategy for promoting homoplasmic plastid transformant production using the barnase–barstar system

(2020) Ayako Okuzaki et al.   Plant Biotechnology

Comparative plastid genomics of Synurophyceae: inverted repeat dynamics and gene content variation

(2019) Jong Im Kim et al.   BMC EVOLUTIONARY BIOLOGY

Tolerance of Transplastomic Tobacco Plants Overexpressing a Theta Class Glutathione Transferase to Abiotic and Oxidative Stresses

(2019) Evangelia Stavridou et al.   Frontiers in Plant Science

RNA Interference in the Tobacco Hornworm, Manduca sexta, Using Plastid-Encoded Long Double-Stranded RNA

(2019) William G. Burke et al.   Frontiers in Plant Science

Evaluation of lettuce chloroplast and soybean cotyledon as platforms for production of functional bone morphogenetic protein 2

(2019) Lídia N. Queiroz et al.   TRANSGENIC RESEARCH

Accumulation of glycine betaine in transplastomic potato plants expressing choline oxidase confers improved drought tolerance

(2019) Lili You et al.   PLANTA

MoChlo: A Versatile, Modular Cloning Toolbox for Chloroplast Biotechnology

(2019) Alessandro Occhialini et al.   PLANT PHYSIOLOGY

Use of the ptxD gene as a portable selectable marker for chloroplast transformation in Chlamydomonas reinhardtii

(2019) José M. Sandoval-Vargas et al.   MOLECULAR BIOTECHNOLOGY

Construction of a species-specific vector for improved plastid transformation efficiency in Capsicum annuum L.

(2019) Srinivas Kota et al.   3 Biotech

Evaluation of gene flow in structured and seed blend refuge systems of non-Bt and Bt corn

(2019) Galen P. Dively et al.   JOURNAL OF PEST SCIENCE

Heat treatment alleviates the growth and photosynthetic impairment of transplastomic plants expressing Leishmania infantum Hsp83-Toxoplasma gondii SAG1 fusion protein

(2019) Mariana G. Corigliano et al.   PLANT SCIENCE

Chloroplast-based inducible expression of ESAT-6 antigen for development of a plant-based vaccine against tuberculosis

(2019) Kiran Saba et al.   JOURNAL OF BIOTECHNOLOGY

The phosphite oxidoreductase gene, ptxD as a bio-contained chloroplast marker and crop-protection tool for algal biotechnology using Chlamydomonas

(2019) Saowalak Changko et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Chloroplast nucleoids are highly dynamic in ploidy, number, and structure during angiosperm leaf development

(2019) Stephan Greiner et al.   PLANT JOURNAL

Stable plastid transformation of rice, a monocot cereal crop

(2018) Yunpeng Wang et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Plastid transformation and its application in metabolic engineering

(2018) Paulina Fuentes et al.   CURRENT OPINION IN BIOTECHNOLOGY

Transplastomic plants yield a multicomponent vaccine against cysticercosis

(2018) Sergio Rosales-Mendoza et al.   JOURNAL OF BIOTECHNOLOGY

Advanced editing of the nuclear and plastid genomes in plants

(2018) Agnieszka A. Piatek et al.   PLANT SCIENCE

Chloroplasts: Structure and Expression of the Plastid Genome

(2018) V. V. Kusnetsov   RUSSIAN JOURNAL OF PLANT PHYSIOLOGY

Engineering the Chloroplast Genome of Oleaginous Marine Microalga Nannochloropsis oceanica

(2018) Qinhua Gan et al.   Frontiers in Plant Science

Construction of chloroplast transformation vector and its functional evaluation in Momordica charantia L.

(2018) Muralikrishna Narra et al.   3 Biotech

Construction of chloroplast transformation vector and its functional evaluation in Momordica charantia L.

(2018) Muralikrishna Narra et al.   3 Biotech

A review of cereal grain proteomics and its potential for sorghum improvement

(2018) M.T. Labuschagne   JOURNAL OF CEREAL SCIENCE

A Ycf2-FtsHi heteromeric AAA-ATPase complex is required for chloroplast protein import

(2018) Shingo Kikuchi et al.   PLANT CELL

Construction of Plastid Expression Vector and Development of Genetic Transformation System for the Seaweed Pyropia yezoensis

(2017) Fanna Kong et al.   MARINE BIOTECHNOLOGY

Ycf1/Tic214 Is Not Essential for the Accumulation of Plastid Proteins

(2017) Bettina Bölter et al.   Molecular Plant

Efficient Plastid Transformation in Arabidopsis

(2017) Qiguo Yu et al.   PLANT PHYSIOLOGY

Plastid genome structure and phylogenomics of Nymphaeales: conserved gene order and new insights into relationships

(2017) Michael Gruenstaeudl et al.   PLANT SYSTEMATICS AND EVOLUTION

Co-Transfer of TALENs Construct Targeted for Chloroplast Genome and Chloroplast Transformation Vector Into Rice Using Particle Bombardment

(2016) Ding Li et al.   JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY

Production and characterization of fungal β-glucosidase and bacterial cellulases by tobacco chloroplast transformation

(2016) Edward Alexander Espinoza-Sánchez et al.   Plant Biotechnology Reports

Transformation of the Cyanidioschyzon merolae chloroplast genome: prospects for understanding chloroplast function in extreme environments

(2016) Maksymilian Zienkiewicz et al.   PLANT MOLECULAR BIOLOGY

A bifunctional aminoglycoside acetyltransferase/phosphotransferase conferring tobramycin resistance provides an efficient selectable marker for plastid transformation

(2016) Iman Tabatabaei et al.   PLANT MOLECULAR BIOLOGY

Analysis of Arabidopsis Accessions Hypersensitive to a Loss of Chloroplast Translation

(2016) Nicole Parker et al.   PLANT PHYSIOLOGY

Comparison of effectiveness of 5′-regulatory sequences in transplastomic tobacco chloroplasts

(2016) I. M. Gerasymenko et al.   TRANSGENIC RESEARCH

In-Plant Protection against Helicoverpa armigera by Production of Long hpRNA in Chloroplasts

(2016) Julia Bally et al.   Frontiers in Plant Science

Complete Chloroplast Genome of Nicotiana otophora and its Comparison with Related Species

(2016) Sajjad Asaf et al.   Frontiers in Plant Science

Stable plastid transformation in Scoparia dulcis L.

(2016) Narra Muralikrishna et al.   PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS

Engineering Plastid Genomes: Methods, Tools, and Applications in Basic Research and Biotechnology

(2015) Ralph Bock   Annual Review of Plant Biology

Translational regulation in chloroplasts for development and homeostasis

(2015) Yi Sun et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Low cost industrial production of coagulation factor IX bioencapsulated in lettuce cells for oral tolerance induction in hemophilia B

(2015) Jin Su et al.   BIOMATERIALS

The fusion ofToxoplasma gondiiSAG1 vaccine candidate toLeishmania infantumheat shock protein 83-kDa improves expression levels in tobacco chloroplasts

(2015) Romina M. Albarracín et al.   Biotechnology Journal

Chloroplast-Expressed MSI-99 in Tobacco Improves Disease Resistance and Displays Inhibitory Effect against Rice Blast Fungus

(2015) Yun-Peng Wang et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Perspectives on transgenic, herbicide-resistant crops in the United States almost 20 years after introduction

(2015) Stephen O Duke   PEST MANAGEMENT SCIENCE

Engineered chloroplast dsRNA silencescytochrome p450 monooxygenase,V-ATPaseandchitin synthasegenes in the insect gut and disruptsHelicoverpa armigeralarval development and pupation

(2015) Shuangxia Jin et al.   PLANT BIOTECHNOLOGY JOURNAL

Oral delivery of Acid Alpha Glucosidase epitopes expressed in plant chloroplasts suppresses antibody formation in treatment of Pompe mice

(2015) Jin Su et al.   PLANT BIOTECHNOLOGY JOURNAL

YCF1: A Green TIC?

(2015) Jan de Vries et al.   PLANT CELL

Full crop protection from an insect pest by expression of long double-stranded RNAs in plastids

(2015) Jiang Zhang et al.   SCIENCE

The fusion ofToxoplasma gondiiSAG1 vaccine candidate toLeishmania infantumheat shock protein 83-kDa improves expression levels in tobacco chloroplasts

(2015) Romina M. Albarracín et al.   Biotechnology Journal

Metabolic engineering of the chloroplast genome reveals that the yeast ArDH gene confers enhanced tolerance to salinity and drought in plants

(2015) Muhammad Sarwar Khan et al.   Frontiers in Plant Science

Suppression of inhibitor formation against FVIII in a murine model of hemophilia A by oral delivery of antigens bioencapsulated in plant cells

(2014) A. Sherman et al.   BLOOD

A Phase 3, multicenter, open-label, switchover trial to assess the safety and efficacy of taliglucerase alfa, a plant cell-expressed recombinant human glucocerebrosidase, in adult and pediatric patients with Gaucher disease previously treated with imiglucerase

(2014) Gregory M. Pastores et al.   BLOOD CELLS MOLECULES AND DISEASES

The green vaccine: A global strategy to combat infectious and autoimmune diseases

(2014) Abdoreza Davoodi-Semiromi et al.   Human vaccines & immunotherapeutics

Oral Delivery of Angiotensin-Converting Enzyme 2 and Angiotensin-(1-7) Bioencapsulated in Plant Cells Attenuates Pulmonary Hypertension

(2014) Vinayak Shenoy et al.   HYPERTENSION

Impact of Enhanced Capacity to Scavenge Reactive Oxygen Species on Cold Tolerance of Tobacco

(2014) Olga M. Grant et al.   INTERNATIONAL JOURNAL OF PLANT SCIENCES

Metabolic engineering of chloroplasts for artemisinic acid biosynthesis and impact on plant growth

(2014) Bhawna Saxena et al.   JOURNAL OF BIOSCIENCES

Construction of Novel Chloroplast Expression Vector and Development of an Efficient Transformation System for the Diatom Phaeodactylum tricornutum

(2014) Wei-Hong Xie et al.   MARINE BIOTECHNOLOGY

Reduction of EPSP synthase in transgenic wild turnip (Brassica rapa) weed via suppression of aroA

(2014) Danial Kahrizi   MOLECULAR BIOLOGY REPORTS

Translational Fusion and Redirection to Thylakoid Lumen as Strategies to Enhance Accumulation of Human Papillomavirus E7 Antigen in Tobacco Chloroplasts

(2014) Mauro Morgenfeld et al.   MOLECULAR BIOTECHNOLOGY

Oral Delivery of ACE2/Ang-(1–7) Bioencapsulated in Plant Cells Protects against Experimental Uveitis and Autoimmune Uveoretinitis

(2014) Pollob K Shil et al.   MOLECULAR THERAPY

TransplastomicNicotiana benthamianaplants expressing multiple defence genes encoding protease inhibitors and chitinase display broad-spectrum resistance against insects, pathogens and abiotic stresses

(2014) Peng-Jen Chen et al.   PLANT BIOTECHNOLOGY JOURNAL

The Largest Plastid Genome of Monocots: a Novel Genome Type Containing AT Residue Repeats in the Slipper Orchid Cypripedium japonicum

(2014) Jung Sung Kim et al.   PLANT MOLECULAR BIOLOGY REPORTER

Expression of chloroperoxidase from Pseudomonas pyrrocinia in tobacco plastids for fungal resistance

(2014) Tracey A. Ruhlman et al.   PLANT SCIENCE

Chloroplast Transformation of Platymonas (Tetraselmis) subcordiformis with the bar Gene as Selectable Marker

(2014) Yulin Cui et al.   PLoS One

Chloroplast-targeted expression of recombinant crystal-protein gene in cotton: An unconventional combat with resistant pests

(2013) Sarfraz Kiani et al.   JOURNAL OF BIOTECHNOLOGY

Oral Delivery of Bioencapsulated Proteins Across Blood–Brain and Blood–Retinal Barriers

(2013) Neha Kohli et al.   MOLECULAR THERAPY

A fusion protein derived from plants holds promising potential as a new oral therapy for type 2 diabetes

(2013) Jeehye Choi et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering cherry rootstocks with resistance to Prunus necrotic ring spot virus through RNAi-mediated silencing

(2013) Guo-qing Song et al.   PLANT BIOTECHNOLOGY JOURNAL

Chloroplast-targeted expression of the codon-optimized truncated cry1Ah gene in transgenic tobacco confers a high level of protection against insects

(2013) Xiuying Li et al.   PLANT CELL REPORTS

Low Cost Tuberculosis Vaccine Antigens in Capsules: Expression in Chloroplasts, Bio-Encapsulation, Stability and Functional Evaluation In Vitro

(2013) Priya Saikumar Lakshmi et al.   PLoS One

Efficient metabolic pathway engineering in transgenic tobacco and tomato plastids with synthetic multigene operons

(2013) Y. Lu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Retraction of article: Stable chloroplast transformation of immature scutella and inflorescences in wheat (Triticum aestivum L.)

(2012) G. He   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

CHLOROPLAST GENETIC TOOL FOR THE GREEN MICROALGAE HAEMATOCOCCUS PLUVIALIS (CHLOROPHYCEAE, VOLVOCALES)1

(2012) Carla L. Gutiérrez et al.   JOURNAL OF PHYCOLOGY

Oral delivery of bioencapsulated exendin-4 expressed in chloroplasts lowers blood glucose level in mice and stimulates insulin secretion in beta-TC6 cells

(2012) Kwang-Chul Kwon et al.   PLANT BIOTECHNOLOGY JOURNAL

Identification ofcis-elements conferring high levels of gene expression in non-green plastids

(2012) Jiang Zhang et al.   PLANT JOURNAL

Design of chimeric expression elements that confer high-level gene activity in chromoplasts

(2012) Rodrigo Caroca et al.   PLANT JOURNAL

Flavodoxin displays dose-dependent effects on photosynthesis and stress tolerance when expressed in transgenic tobacco plants

(2012) Romina D. Ceccoli et al.   PLANTA

Characterizing the Mechanism of Action of Double-Stranded RNA Activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)

(2012) Renata Bolognesi et al.   PLoS One

Horizontal transfer of chloroplast genomes between plant species

(2012) S. Stegemann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plastid genetic engineering in Solanaceae

(2012) Jelli Venkatesh et al.   PROTOPLASMA

Production of recombinant enzymes in the marine alga Dunaliella tertiolecta

(2012) D. Ryan Georgianna et al.   Algal Research-Biomass Biofuels and Bioproducts

Unsolved problems in plastid transformation

(2012) M. Manuela Rigano et al.   Bioengineered

Stable chloroplast transformation of immature scutella and inflorescences in wheat (Triticum aestivum L.)

(2011) C. Cui et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

Transformation of alfalfa chloroplasts and expression of green fluorescent protein in a forage crop

(2011) Zhengyi Wei et al.   BIOTECHNOLOGY LETTERS

Independent effects of leaf growth and light on the development of the plastid and its DNA content in Zea species

(2011) Qi Zheng et al.   JOURNAL OF EXPERIMENTAL BOTANY

The chloroplast transformation toolbox: selectable markers and marker removal

(2011) Anil Day et al.   PLANT BIOTECHNOLOGY JOURNAL

Tobacco chloroplast transformants expressing genes encoding dehydroascorbate reductase, glutathione reductase, and glutathione-S-transferase, exhibit altered anti-oxidant metabolism and improved abiotic stress tolerance

(2011) Bénédicte Le Martret et al.   PLANT BIOTECHNOLOGY JOURNAL

Pinellia ternata agglutinin expression in chloroplasts confers broad spectrum resistance against aphid, whitefly, Lepidopteran insects, bacterial and viral pathogens

(2011) Shuangxia Jin et al.   PLANT BIOTECHNOLOGY JOURNAL

Plastid expression of a double-pentameric vaccine candidate containing human papillomavirus-16 L1 antigen fused with LTB as adjuvant: transplastomic plants show pleiotropic phenotypes

(2011) Mohammad T. Waheed et al.   PLANT BIOTECHNOLOGY JOURNAL

Development of chloroplast transformation vectors, and a new target region in the tobacco plastid genome

(2011) Raju Madanala et al.   Plant Biotechnology Reports

Chloroplast genomes of photosynthetic eukaryotes

(2011) Beverley R. Green   PLANT JOURNAL

Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation

(2011) Miranda Poage et al.   PLANT MOLECULAR BIOLOGY

High-level expression of a suite of thermostable cell wall-degrading enzymes from the chloroplast genome

(2011) Kerstin Petersen et al.   PLANT MOLECULAR BIOLOGY

Development of insect-resistant transgenic cotton with chimeric TVip3A* accumulating in chloroplasts

(2011) Jiahe Wu et al.   TRANSGENIC RESEARCH

Geological constraints on the origin of oxygenic photosynthesis

(2010) James Farquhar et al.   PHOTOSYNTHESIS RESEARCH

Chloroplast transformation of rapeseed (Brassica napus) by particle bombardment of cotyledons

(2010) Lin Cheng et al.   PLANT CELL REPORTS

The effect of different 3′ untranslated regions on the accumulation and stability of transcripts of a gfp transgene in chloroplasts of transplastomic tobacco

(2010) Sithichoke Tangphatsornruang et al.   PLANT MOLECULAR BIOLOGY

Chloramphenicol acetyltransferase as selectable marker for plastid transformation

(2010) Weimin Li et al.   PLANT MOLECULAR BIOLOGY

Release of Hormones from Conjugates: Chloroplast Expression of  -Glucosidase Results in Elevated Phytohormone Levels Associated with Significant Increase in Biomass and Protection from Aphids or Whiteflies Conferred by Sucrose Esters

(2010) S. Jin et al.   PLANT PHYSIOLOGY

The Role of Heterologous Chloroplast Sequence Elements in Transgene Integration and Expression

(2010) T. Ruhlman et al.   PLANT PHYSIOLOGY

Inducible gene expression from the plastid genome by a synthetic riboswitch

(2010) A. Verhounig et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High efficiency plastid transformation in potato and regulation of transgene expression in leaves and tubers by alternative 5′ and 3′ regulatory sequences

(2010) Vladimir T. Valkov et al.   TRANSGENIC RESEARCH

Cotton plants expressing CYP6AE14 double-stranded RNA show enhanced resistance to bollworms

(2010) Ying-Bo Mao et al.   TRANSGENIC RESEARCH

Transplastomic expression of a modified human papillomavirus L1 protein leading to the assembly of capsomeres in tobacco: a step towards cost-effective second-generation vaccines

(2010) M. Tahir Waheed et al.   TRANSGENIC RESEARCH

Production of recombinant proteins by microbes and higher organisms

(2009) Arnold L. Demain et al.   BIOTECHNOLOGY ADVANCES

Stable Plastid Transformation in Nicotiana benthamiana

(2009) Seyed Javad Davarpanah et al.   JOURNAL OF PLANT BIOLOGY

Cholera toxin B suppresses allergic inflammation through induction of secretory IgA

(2009) H H Smits et al.   Mucosal Immunology

Transgenic chloroplasts are efficient sites for high-yield production of the vaccinia virus envelope protein A27L in plant cells

(2009) M. Manuela Rigano et al.   PLANT BIOTECHNOLOGY JOURNAL

Genome-Wide Analysis of Plastid Gene Expression in Potato Leaf Chloroplasts and Tuber Amyloplasts: Transcriptional and Posttranscriptional Control

(2009) V. T. Valkov et al.   PLANT PHYSIOLOGY

High-level expression of the HIV-1 Pr55gag polyprotein in transgenic tobacco chloroplasts

(2009) Nunzia Scotti et al.   PLANTA

Plastid transformation in eggplant (Solanum melongena L.)

(2009) A. K. Singh et al.   TRANSGENIC RESEARCH

The give-and-take of DNA: horizontal gene transfer in plants

(2009) Ralph Bock   TRENDS IN PLANT SCIENCE

Gene rearrangement analysis and ancestral order inference from chloroplast genomes with inverted repeat

(2008) Feng Yue et al.   BMC GENOMICS

High-level expression of human immunodeficiency virus antigens from the tobacco and tomato plastid genomes

(2008) Fei Zhou et al.   PLANT BIOTECHNOLOGY JOURNAL

Translocation of aprotinin, a therapeutic protease inhibitor, into the thylakoid lumen of genetically engineered tobacco chloroplasts

(2008) Ghislaine Tissot et al.   PLANT BIOTECHNOLOGY JOURNAL

Plastid-expressed choline monooxygenase gene improves salt and drought tolerance through accumulation of glycine betaine in tobacco

(2008) Jiang Zhang et al.   PLANT CELL REPORTS

Biosynthesis of astaxanthin in tobacco leaves by transplastomic engineering

(2008) Tomohisa Hasunuma et al.   PLANT JOURNAL

Selectable Tolerance to Herbicides by Mutated Acetolactate Synthase Genes Integrated into the Chloroplast Genome of Tobacco

(2008) M. Shimizu et al.   PLANT PHYSIOLOGY

Expression of a Bacillus thuringiensis toxin (cry1Ab) gene in cabbage (Brassica oleracea L. var. capitata L.) chloroplasts confers high insecticidal efficacy against Plutella xylostella

(2008) Cheng-Wei Liu et al.   THEORETICAL AND APPLIED GENETICS

Genetic transformation of the sugar beet plastome

(2008) Francesca De Marchis et al.   TRANSGENIC RESEARCH