Unsolved problems in plastid transformation

Increased accumulation and stability of rotavirus VP6 protein in tobacco chloroplasts following changes to the 5′ untranslated region and the 5′ end of the coding region

(2012) A.-M. Inka Borchers et al.   PLANT BIOTECHNOLOGY JOURNAL

Identification ofcis-elements conferring high levels of gene expression in non-green plastids

(2012) Jiang Zhang et al.   PLANT JOURNAL

Visual marker and Agrobacterium-delivered recombinase enable the manipulation of the plastid genome in greenhouse-grown tobacco plants

(2012) Tarinee Tungsuchat-Huang et al.   PLANT JOURNAL

Exceptional inheritance of plastids via pollen inNicotiana sylvestriswith no detectable paternal mitochondrial DNA in the progeny

(2012) Gregory Thyssen et al.   PLANT JOURNAL

Translational fusion and redirection to thylakoid lumen as strategies to improve the accumulation of a camelid antibody fragment in transplastomic tobacco

(2012) Ezequiel M. Lentz et al.   PLANTA

Production of foreign proteins using plastid transformation

(2011) Nunzia Scotti et al.   BIOTECHNOLOGY ADVANCES

Transformation of alfalfa chloroplasts and expression of green fluorescent protein in a forage crop

(2011) Zhengyi Wei et al.   BIOTECHNOLOGY LETTERS

A synthetic gene increases TGFβ3 accumulation by 75-fold in tobacco chloroplasts enabling rapid purification and folding into a biologically active molecule

(2011) Martin F. Gisby et al.   PLANT BIOTECHNOLOGY JOURNAL

Plastid expression of a double-pentameric vaccine candidate containing human papillomavirus-16 L1 antigen fused with LTB as adjuvant: transplastomic plants show pleiotropic phenotypes

(2011) Mohammad T. Waheed et al.   PLANT BIOTECHNOLOGY JOURNAL

Chloroplast-derived vaccines against human diseases: achievements, challenges and scopes

(2011) Andreas G. Lössl et al.   PLANT BIOTECHNOLOGY JOURNAL

Optimization of the expression of the HIV fusion inhibitor cyanovirin-N from the tobacco plastid genome

(2011) Zouhair Elghabi et al.   PLANT BIOTECHNOLOGY JOURNAL

Biolistic co-transformation of the nuclear and plastid genomes

(2011) Zouhair Elghabi et al.   PLANT JOURNAL

An efficient downstream box fusion allows high-level accumulation of active bacterial beta-glucosidase in tobacco chloroplasts

(2011) Benjamin N. Gray et al.   PLANT MOLECULAR BIOLOGY

Plastid Biotechnology: Food, Fuel, and Medicine for the 21st Century

(2011) P. Maliga et al.   PLANT PHYSIOLOGY

Metabolic Adaptation in Transplastomic Plants Massively Accumulating Recombinant Proteins

(2011) Julia Bally et al.   PLoS One

Chloroplasts as expression platforms for plant-produced vaccines

(2010) Teodoro Cardi et al.   Expert Review of Vaccines

Identification of protein stability determinants in chloroplasts

(2010) Wiebke Apel et al.   PLANT JOURNAL

A plant secretory signal peptide targets plastome-encoded recombinant proteins to the thylakoid membrane

(2010) Francesca De Marchis et al.   PLANT MOLECULAR BIOLOGY

The Role of Heterologous Chloroplast Sequence Elements in Transgene Integration and Expression

(2010) T. Ruhlman et al.   PLANT PHYSIOLOGY

Inducible gene expression from the plastid genome by a synthetic riboswitch

(2010) A. Verhounig et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High efficiency plastid transformation in potato and regulation of transgene expression in leaves and tubers by alternative 5′ and 3′ regulatory sequences

(2010) Vladimir T. Valkov et al.   TRANSGENIC RESEARCH

Production of recombinant proteins by microbes and higher organisms

(2009) Arnold L. Demain et al.   BIOTECHNOLOGY ADVANCES

Transgenic chloroplasts are efficient sites for high-yield production of the vaccinia virus envelope protein A27L in plant cells

(2009) M. Manuela Rigano et al.   PLANT BIOTECHNOLOGY JOURNAL

Genome-Wide Analysis of Plastid Gene Expression in Potato Leaf Chloroplasts and Tuber Amyloplasts: Transcriptional and Posttranscriptional Control

(2009) V. T. Valkov et al.   PLANT PHYSIOLOGY

High-level expression of the HIV-1 Pr55gag polyprotein in transgenic tobacco chloroplasts

(2009) Nunzia Scotti et al.   PLANTA

High-level expression of human immunodeficiency virus antigens from the tobacco and tomato plastid genomes

(2008) Fei Zhou et al.   PLANT BIOTECHNOLOGY JOURNAL

Plastid Transcriptomics and Translatomics of Tomato Fruit Development and Chloroplast-to-Chromoplast Differentiation: Chromoplast Gene Expression Largely Serves the Production of a Single Protein

(2008) S. Kahlau et al.   PLANT CELL

Exhaustion of the chloroplast protein synthesis capacity by massive expression of a highly stable protein antibiotic

(2008) Melanie Oey et al.   PLANT JOURNAL

Translational fusion of chloroplast-expressed human papillomavirus type 16 L1 capsid protein enhances antigen accumulation in transplastomic tobacco

(2008) Paolo Lenzi et al.   TRANSGENIC RESEARCH