Production of isotope-labeled proteins in insect cells for NMR

An improved protocol for amino acid type-selective isotope labeling in insect cells

(2017) Yaqun Zhang et al.   JOURNAL OF BIOMOLECULAR NMR

Insight into partial agonism by observing multiple equilibria for ligand-bound and Gs-mimetic nanobody-bound β1-adrenergic receptor

(2017) Andras S. Solt et al.   Nature Communications

Backbone NMR reveals allosteric signal transduction networks in the β1-adrenergic receptor

(2016) Shin Isogai et al.   NATURE

Identification of a Conformational Equilibrium That Determines the Efficacy and Functional Selectivity of the μ-Opioid Receptor

(2015) Junya Okude et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Affordable uniform isotope labeling with 2H, 13C and 15N in insect cells

(2015) Agnieszka Sitarska et al.   JOURNAL OF BIOMOLECULAR NMR

An economic approach to efficient isotope labeling in insect cells using homemade 15N-, 13C- and 2H-labeled yeast extracts

(2015) Christian Opitz et al.   JOURNAL OF BIOMOLECULAR NMR

Functional Dynamics of Deuterated β2-Adrenergic Receptor in Lipid Bilayers Revealed by NMR Spectroscopy

(2014) Yutaka Kofuku et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Robust and low cost uniform 15N-labeling of proteins expressed in Drosophila S2 cells and Spodoptera frugiperda Sf9 cells for NMR applications

(2014) Annalisa Meola et al.   JOURNAL OF STRUCTURAL BIOLOGY

The Dynamic Process of β2-Adrenergic Receptor Activation

(2013) Rie Nygaard et al.   CELL

Efficacy of the β2-adrenergic receptor is determined by conformational equilibrium in the transmembrane region

(2012) Yutaka Kofuku et al.   Nature Communications

A simple protocol for amino acid type selective isotope labeling in insect cells with improved yields and high reproducibility

(2011) Alvar D. Gossert et al.   JOURNAL OF BIOMOLECULAR NMR

Stable isotope labelling of human histamine receptor H1R: Prospects for structure-based drug design

(2010) T. A. Egorova-Zachernyuk et al.   Doklady Biochemistry and Biophysics

Production of yeastolates for uniform stable isotope labelling in eukaryotic cell culture

(2009) T. A. Egorova-Zachernyuk et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The titerless infected-cells preservation and scale-up (TIPS) method for large-scale production of NO-sensitive human soluble guanylate cyclase (sGC) from insect cells infected with recombinant baculovirus

(2009) David J. Wasilko et al.   PROTEIN EXPRESSION AND PURIFICATION

Backbone NMR resonance assignment of the Abelson kinase domain in complex with imatinib

(2008) Navratna Vajpai et al.   Biomolecular NMR Assignments

Generation of baculovirus vectors for the high-throughput production of proteins in insect cells

(2008) Robert D. Possee et al.   BIOTECHNOLOGY AND BIOENGINEERING

Solution Conformations and Dynamics of ABL Kinase-Inhibitor Complexes Determined by NMR Substantiate the Different Binding Modes of Imatinib/Nilotinib and Dasatinib

(2008) Navratna Vajpai et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Photobioreactors for mass cultivation of algae

(2007) C.U. Ugwu et al.   BIORESOURCE TECHNOLOGY