Ex vivo cultures and drug testing of primary acute myeloid leukemia samples: Current techniques and implications for experimental design and outcome

Tyrosine kinase inhibitors for acute myeloid leukemia: A step toward disease control?

(2020) Juan Eduardo Megías-Vericat et al.   BLOOD REVIEWS

Phosphotyrosine-based phosphoproteomics for target identification and drug response prediction in AML cell lines

(2020) Carolien van Alphen et al.   MOLECULAR & CELLULAR PROTEOMICS

Extracellular Vesicles and Chemotherapy Resistance in the AML Microenvironment

(2020) Jill Nehrbas et al.   Frontiers in Oncology

3D Extracellular Matrix Mimics: Fundamental Concepts and Role of Materials Chemistry to Influence Stem Cell Fate

(2020) Julien Nicolas et al.   BIOMACROMOLECULES

Theranostic drug test incorporating the bone‐marrow microenvironment can predict the clinical response of acute myeloid leukaemia to chemotherapy

(2020) Sukhraj Pal S. Dhami et al.   BRITISH JOURNAL OF HAEMATOLOGY

Genetic biomarkers of drug resistance: A compass of prognosis and targeted therapy in acute myeloid leukemia

(2020) Luyao Long et al.   DRUG RESISTANCE UPDATES

Venetoclax combines synergistically with FLT3 inhibition to effectively target leukemic cells in FLT3-ITD+ acute myeloid leukemia models

(2020) Raghuveer Singh Mali et al.   HAEMATOLOGICA

Disulfiram/cytarabine eradicates a subset of acute myeloid leukemia stem cells with high aldehyde dehydrogenase expression

(2020) Wanfang Yang et al.   LEUKEMIA RESEARCH

Endothelial E-selectin inhibition improves acute myeloid leukaemia therapy by disrupting vascular niche-mediated chemoresistance

(2020) Valerie Barbier et al.   Nature Communications

Location First: Targeting Acute Myeloid Leukemia Within Its Niche

(2020) Alice Pievani et al.   Journal of Clinical Medicine

Harnessing Gene Expression Profiles for the Identification of Ex Vivo Drug Response Genes in Pediatric Acute Myeloid Leukemia

(2020) David G.J. Cucchi et al.   Cancers

A Feeder-Free and Serum-Free In Vitro Assay for Measuring the Effect of Drugs on Acute and Chronic Myeloid Leukemia Stem/Progenitor Cells

(2020) Hanyang Lin et al.   EXPERIMENTAL HEMATOLOGY

Repurposing old drugs to fight multidrug resistant cancers

(2020) Jelena Dinić et al.   DRUG RESISTANCE UPDATES

Are we witnessing the start of a therapeutic revolution in acute myeloid leukemia?

(2019) Jan Philipp Bewersdorf et al.   LEUKEMIA & LYMPHOMA

Considerations for high-yield, high-throughput cell enrichment: fluorescence versus magnetic sorting

(2019) Bryan A. Sutermaster et al.   Scientific Reports

The Capacity of Long-Term in Vitro Proliferation of Acute Myeloid Leukemia Cells Supported Only by Exogenous Cytokines Is Associated with a Patient Subset with Adverse Outcome

(2019) Annette K. Brenner et al.   Cancers

Genetic mechanisms of primary chemotherapy resistance in pediatric acute myeloid leukemia

(2019) Nicole A. McNeer et al.   LEUKEMIA

Regulation of drug metabolizing enzymes in the leukaemic bone marrow microenvironment

(2019) Meng Su et al.   JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

Hematopoietic niche drives FLT3-ITD acute myeloid leukemia resistance to quizartinib via STAT5- and hypoxia- dependent up-regulation of AXL

(2019) Pierre-Yves Dumas et al.   HAEMATOLOGICA

Surmounting Cytarabine-resistance in acute myeloblastic leukemia cells and specimens with a synergistic combination of hydroxyurea and azidothymidine

(2019) May Levin et al.   Cell Death & Disease

Clonal selection with Ras pathway activation mediates secondary clinical resistance to selective FLT3 inhibition in acute myeloid leukemia

(2019) Christine M. McMahon et al.   Cancer Discovery

Phenotype-based drug screening reveals association between venetoclax response and differentiation stage in acute myeloid leukemia

(2019) Heikki Kuusanmäki et al.   HAEMATOLOGICA

Healthcare expenses for treatment of acute myeloid leukemia

(2019) Jan Philipp Bewersdorf et al.   Expert Review of Hematology

Moving Myeloid Leukemia Drug Discovery Into the Third Dimension

(2019) Donna M. Cartledge Wolf et al.   Frontiers in Pediatrics

Metabolic Plasticity of Acute Myeloid Leukemia

(2019) Kreitz et al.   Cells

The multi-factorial nature of clinical multidrug resistance in cancer

(2019) Yehuda G. Assaraf et al.   DRUG RESISTANCE UPDATES

Bone marrow mesenchymal stromal cells from acute myelogenous leukemia patients demonstrate adipogenic differentiation propensity with implications for leukemia cell support

(2019) Mitra Azadniv et al.   LEUKEMIA

Overcoming adaptive therapy resistance in AML by targeting immune response pathways

(2019) Katelyn Melgar et al.   Science Translational Medicine

Gilteritinib or Chemotherapy for Relapsed or Refractory FLT3-Mutated AML

(2019) Alexander E. Perl et al.   NEW ENGLAND JOURNAL OF MEDICINE

Inhibition of mitochondrial translation overcomes venetoclax resistance in AML through activation of the integrated stress response

(2019) David Sharon et al.   Science Translational Medicine

Acute Promyelocytic Leukemia: Update on the Mechanisms of Leukemogenesis, Resistance and on Innovative Treatment Strategies

(2019) N. I. Noguera et al.   Cancers

Advanced technological tools to study multidrug resistance in cancer

(2019) Luca Andrei et al.   DRUG RESISTANCE UPDATES

Acadesine Circumvents Azacitidine Resistance in Myelodysplastic Syndrome and Acute Myeloid Leukemia

(2019) Thomas Cluzeau et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

The role of exosomes and MYC in therapy resistance of acute myeloid leukemia: Challenges and opportunities

(2019) Nithya Mudgapalli et al.   MOLECULAR ASPECTS OF MEDICINE

MEK inhibition enhances the response to tyrosine kinase inhibitors in acute myeloid leukemia

(2019) María Luz Morales et al.   Scientific Reports

Current approaches in biomaterial-based hematopoietic stem cell niches

(2018) Alvin Bacero Bello et al.   Acta Biomaterialia

RNA-basedFLT3-ITD allelic ratio is associated with outcome and ex vivo response to FLT3 inhibitors in pediatric AML

(2018) David G. J. Cucchi et al.   BLOOD

Advances in treatment formulations for acute myeloid leukemia

(2018) Thomas Briot et al.   DRUG DISCOVERY TODAY

Roles of the bone marrow niche in hematopoiesis, leukemogenesis, and chemotherapy resistance in acute myeloid leukemia

(2018) Andi Wang et al.   Hematology

Ex-vivo sensitivity profiling to guide clinical decision making in acute myeloid leukemia: A pilot study

(2018) Ronan T. Swords et al.   LEUKEMIA RESEARCH

Durable Remissions with Ivosidenib in IDH1-Mutated Relapsed or Refractory AML

(2018) Courtney D. DiNardo et al.   NEW ENGLAND JOURNAL OF MEDICINE

Role of drug transporters in the sensitivity of acute myeloid leukemia to sorafenib

(2018) Rocio I.R. Macias et al.   Oncotarget

Engineering a multicellular vascular niche to model hematopoietic cell trafficking

(2018) Surya S. Kotha et al.   Stem Cell Research & Therapy

Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning

(2018) Sigrid A. Langhans   Frontiers in Pharmacology

Prediction of acute myeloid leukaemia risk in healthy individuals

(2018) Sagi Abelson et al.   NATURE

Oxygenation in cell culture: Critical parameters for reproducibility are routinely not reported

(2018) Abdullah Al-Ani et al.   PLoS One

3D models of the bone marrow in health and disease: yesterday, today, and tomorrow

(2018) Annamarija Raic et al.   MRS Communications

Immunotherapy in acute myeloid leukemia and myelodysplastic syndromes: The dawn of a new era?

(2018) Yuxin Liu et al.   BLOOD REVIEWS

Evidence of a role for functional heterogeneity in multidrug resistance transporters in clinical trials of P-glycoprotein modulation in acute myeloid leukemia

(2018) John F. Marcelletti et al.   CYTOMETRY PART B-CLINICAL CYTOMETRY

Single cell analysis of clonal architecture in acute myeloid leukaemia

(2018) Nicola Potter et al.   LEUKEMIA

Functional genomic landscape of acute myeloid leukaemia

(2018) Jeffrey W. Tyner et al.   NATURE

Acute Myeloid Leukemia and the Bone Marrow Niche—Take a Closer Look

(2018) Lena Behrmann et al.   Frontiers in Oncology

Cytarabine and daunorubicin for the treatment of acute myeloid leukemia

(2017) Tracy Murphy et al.   EXPERT OPINION ON PHARMACOTHERAPY

High-throughput profiling of signaling networks identifies mechanism-based combination therapy to eliminate microenvironmental resistance in acute myeloid leukemia

(2017) Zhihong Zeng et al.   HAEMATOLOGICA

A three-dimensional ex vivo tri-culture model mimics cell-cell interactions between acute myeloid leukemia and the vascular niche

(2017) Laura J. Bray et al.   HAEMATOLOGICA

Gilteritinib, a FLT3/AXL inhibitor, shows antileukemic activity in mouse models of FLT3 mutated acute myeloid leukemia

(2017) Masamichi Mori et al.   INVESTIGATIONAL NEW DRUGS

Molecular and genetic alterations associated with therapy resistance and relapse of acute myeloid leukemia

(2017) Hubert Hackl et al.   Journal of Hematology & Oncology

Novel Method Enabling the Use of Cryopreserved Primary Acute Myeloid Leukemia Cells in Functional Drug Screens

(2017) Michelle Degnin et al.   JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY

Drugs with anti-oxidant properties can interfere with cell viability measurements by assays that rely on the reducing property of viable cells

(2017) Niraj Shenoy et al.   LABORATORY INVESTIGATION

FLT3–ITD and its current role in acute myeloid leukaemia

(2017) Francisco Alejandro Lagunas-Rangel et al.   MEDICAL ONCOLOGY

Midostaurin plus Chemotherapy for Acute Myeloid Leukemia with a FLT3 Mutation

(2017) Richard M. Stone et al.   NEW ENGLAND JOURNAL OF MEDICINE

Signaling mechanisms that regulate ex vivo survival of human acute myeloid leukemia initiating cells

(2017) Dheeraj Bhavanasi et al.   Blood Cancer Journal

A phase 1/2 study of chemosensitization with plerixafor plus G-CSF in relapsed or refractory acute myeloid leukemia

(2017) G L Uy et al.   Blood Cancer Journal

Chemotherapy-Resistant Human Acute Myeloid Leukemia Cells Are Not Enriched for Leukemic Stem Cells but Require Oxidative Metabolism

(2017) Thomas Farge et al.   Cancer Discovery

Novel strategies to prevent the development of multidrug resistance (MDR) in cancer

(2017) Jinglu Wang et al.   Oncotarget

Resistance of leukemia cells to cytarabine chemotherapy is mediated by bone marrow stroma, involves cell-surface equilibrative nucleoside transporter-1 removal and correlates with patient outcome

(2017) Patricia Macanas-Pirard et al.   Oncotarget

3D models of the hematopoietic stem cell niche under steady-state and active conditions

(2017) Lisa Rödling et al.   Scientific Reports

Human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves

(2017) Jonathan M. Grasman et al.   Scientific Reports

High-throughput profiling of signaling networks identifies mechanism-based combination therapy to eliminate microenvironmental resistance in acute myeloid leukemia

(2017) Zhihong Zeng et al.   HAEMATOLOGICA

A three-dimensional ex vivo tri-culture model mimics cell-cell interactions between acute myeloid leukemia and the vascular niche

(2017) Laura J. Bray et al.   HAEMATOLOGICA

Optimizing recovery of frozen human peripheral blood mononuclear cells for flow cytometry

(2017) Bo Langhoff Hønge et al.   PLoS One

Use antibiotics in cell culture with caution: genome-wide identification of antibiotic-induced changes in gene expression and regulation

(2017) Ann H. Ryu et al.   Scientific Reports

Establishing human leukemia xenograft mouse models by implanting human bone marrow-like scaffold-based niches

(2016) A. Antonelli et al.   BLOOD

FGF2 from Marrow Microenvironment Promotes Resistance to FLT3 Inhibitors in Acute Myeloid Leukemia

(2016) Elie Traer et al.   CANCER RESEARCH

Small-molecule Hedgehog inhibitor attenuates the leukemia-initiation potential of acute myeloid leukemia cells

(2016) Nobuaki Fukushima et al.   CANCER SCIENCE

Three-dimensional ex vivo co-culture models of the leukaemic bone marrow niche for functional drug testing

(2016) Sukhraj Pal S. Dhami et al.   DRUG DISCOVERY TODAY

Liquid-based three-dimensional tumor models for cancer research and drug discovery

(2016) Stephanie L Ham et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Changes of heterogeneous cell populations in the Ishikawa cell line during long-term culture: Proposal for an in vitro clonal evolution model of tumor cells

(2016) Fumio Kasai et al.   GENOMICS

MicroRNA-494 Activation Suppresses Bone Marrow Stromal Cell-Mediated Drug Resistance in Acute Myeloid Leukemia Cells

(2016) Chen Tian et al.   JOURNAL OF CELLULAR PHYSIOLOGY

Immunoprofiling of leukemic stem cells CD34+/CD38−/CD123+ delineate FLT3/ITD-positive clones

(2016) Adhra Al-Mawali et al.   Journal of Hematology & Oncology

Freezing effects on the acute myeloid leukemia cell proteome and phosphoproteome revealed using optimal quantitative workflows

(2016) Elise Aasebø et al.   Journal of Proteomics

SAMHD1 is a biomarker for cytarabine response and a therapeutic target in acute myeloid leukemia

(2016) Constanze Schneider et al.   NATURE MEDICINE

Extracellular matrix stiffness causes systematic variations in proliferation and chemosensitivity in myeloid leukemias

(2016) Jae-Won Shin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Tyrosine kinase inhibitors and mesenchymal stromal cells: effects on self-renewal, commitment and functions

(2016) Adriana Borriello et al.   Oncotarget

Bone marrow niche-mediated survival of leukemia stem cells in acute myeloid leukemia: Yin and Yang

(2016) Hong-Sheng Zhou et al.   Cancer Biology & Medicine

The oncometabolite R-2-hydroxyglutarate activates NF-κB-dependent tumor-promoting stromal niche for acute myeloid leukemia cells

(2016) Jing-Yi Chen et al.   Scientific Reports

Primary acute myeloid leukemia cells with overexpression of EVI-1 are sensitive to all-trans retinoic acid

(2015) H. J. M. P. Verhagen et al.   BLOOD

Methotrexate resistance in relation to treatment outcome in childhood acute lymphoblastic leukemia

(2015) Anna Wojtuszkiewicz et al.   Journal of Hematology & Oncology

c-Myc plays part in drug resistance mediated by bone marrow stromal cells in acute myeloid leukemia

(2015) Bing Xia et al.   LEUKEMIA RESEARCH

Synthetic lethal targeting of oncogenic transcription factors in acute leukemia by PARP inhibitors

(2015) Maria Teresa Esposito et al.   NATURE MEDICINE

Long term maintenance of myeloid leukemic stem cells cultured with unrelated human mesenchymal stromal cells

(2015) Sawa Ito et al.   Stem Cell Research

Role of Microenvironment in Resistance to Therapy in AML

(2015) Yoko Tabe et al.   Current Hematologic Malignancy Reports

Targeting the Microenvironment in Acute Myeloid Leukemia

(2015) Armin Rashidi et al.   Current Hematologic Malignancy Reports

Proteomic analysis of the response to cell cycle arrests in human myeloid leukemia cells

(2015) Tony Ly et al.   eLife

Peptide Hydrogels – Versatile Matrices for 3D Cell Culture in Cancer Medicine

(2015) Peter Worthington et al.   Frontiers in Oncology

Modification of Gene Expression, Proliferation, and Function of OP9 Stroma Cells by Bcr-Abl-Expressing Leukemia Cells

(2015) Emmanuelle Supper et al.   PLoS One

Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors

(2014) Rasheena Edmondson et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Reciprocal leukemia-stroma VCAM-1/VLA-4-dependent activation of NF- B mediates chemoresistance

(2014) R. Jacamo et al.   BLOOD

Functional Heterogeneity of Genetically Defined Subclones in Acute Myeloid Leukemia

(2014) Jeffery M. Klco et al.   CANCER CELL

Cytokines secreted by bone marrow stromal cells protect c-KIT mutant AML cells from c-KIT inhibitor-induced apoptosis

(2014) P M Gordon et al.   LEUKEMIA

Identification of small molecules that support human leukemia stem cell activity ex vivo

(2014) Caroline Pabst et al.   NATURE METHODS

Pyrimidoindole derivatives are agonists of human hematopoietic stem cell self-renewal

(2014) I. Fares et al.   SCIENCE

Quantitative scoring of differential drug sensitivity for individually optimized anticancer therapies

(2014) Bhagwan Yadav et al.   Scientific Reports

A Niche-Like Culture System Allowing the Maintenance of Primary Human Acute Myeloid Leukemia-Initiating Cells: A New Tool to Decipher Their Chemoresistance and Self-Renewal Mechanisms

(2014) Emmanuel Griessinger et al.   Stem Cells Translational Medicine

Bone marrow stroma-mediated resistance to FLT3 inhibitors in FLT3-ITD AML is mediated by persistent activation of extracellular regulated kinase

(2013) Xiaochuan Yang et al.   BRITISH JOURNAL OF HAEMATOLOGY

A method for evaluating the use of fluorescent dyes to track proliferation in cell lines by dye dilution

(2013) Julfa Begum et al.   CYTOMETRY PART A

Tumor heterogeneity makes AML a “moving target” for detection of residual disease

(2013) W. Zeijlemaker et al.   CYTOMETRY PART B-CLINICAL CYTOMETRY

Current practices and prospects for standardization of the hematopoietic colony-forming unit assay: a report by the cellular therapy team of the Biomedical Excellence for Safer Transfusion (BEST) Collaborative

(2013) Derwood Pamphilon et al.   CYTOTHERAPY

A novel three-dimensional stromal-based model forin vitrochemotherapy sensitivity testing of leukemia cells

(2013) Omar S. Aljitawi et al.   LEUKEMIA & LYMPHOMA

Chromatin proteins and modifications as drug targets

(2013) Kristian Helin et al.   NATURE

Phase II and Phase III attrition rates 2011–2012

(2013) John Arrowsmith et al.   NATURE REVIEWS DRUG DISCOVERY

Selective BCL-2 Inhibition by ABT-199 Causes On-Target Cell Death in Acute Myeloid Leukemia

(2013) R. Pan et al.   Cancer Discovery

A novel function for the haemopoietic supportive murine bone marrow MS-5 mesenchymal stromal cell line in promoting human vasculogenesis and angiogenesis

(2012) Bob Zhou et al.   BRITISH JOURNAL OF HAEMATOLOGY

The role of minor subpopulations within the leukemic blast compartment of AML patients at initial diagnosis in the development of relapse

(2012) C Bachas et al.   LEUKEMIA

Raise standards for preclinical cancer research

(2012) C. Glenn Begley et al.   NATURE

Bone Marrow Stromal Cells Modulate Mouse ENT1 Activity and Protect Leukemia Cells from Cytarabine Induced Apoptosis

(2012) Patricia Macanas-Pirard et al.   PLoS One

Optimal Thawing of Cryopreserved Peripheral Blood Mononuclear Cells for Use in High-Throughput Human Immune Monitoring Studies

(2012) Hari Ramachandran et al.   Cells

p53 activation of mesenchymal stromal cells partially abrogates microenvironment-mediated resistance to FLT3 inhibition in AML through HIF-1 -mediated down-regulation of CXCL12

(2011) K. Kojima et al.   BLOOD

FLT3 ligand impedes the efficacy of FLT3 inhibitors in vitro and in vivo

(2011) T. Sato et al.   BLOOD

Bone Marrow Stroma-Secreted Cytokines Protect JAK2V617F-Mutated Cells from the Effects of a JAK2 Inhibitor

(2011) T. Manshouri et al.   CANCER RESEARCH

Shortcomings in the clinical evaluation of new drugs: acute myeloid leukemia as paradigm

(2010) R. B. Walter et al.   BLOOD

Cell death in mammalian cell culture: molecular mechanisms and cell line engineering strategies

(2010) Britta Krampe et al.   CYTOTECHNOLOGY

Aryl Hydrocarbon Receptor Antagonists Promote the Expansion of Human Hematopoietic Stem Cells

(2010) Anthony E. Boitano et al.   SCIENCE

The development of a three-dimensional scaffold for ex vivo biomimicry of human acute myeloid leukaemia

(2009) Teresa Mortera Blanco et al.   BIOMATERIALS

Hydrogels as extracellular matrix mimics for 3D cell culture

(2009) Mark W. Tibbitt et al.   BIOTECHNOLOGY AND BIOENGINEERING

Targeting the leukemia microenvironment by CXCR4 inhibition overcomes resistance to kinase inhibitors and chemotherapy in AML

(2008) Z. Zeng et al.   BLOOD

Chemosensitization of acute myeloid leukemia (AML) following mobilization by the CXCR4 antagonist AMD3100

(2008) B. Nervi et al.   BLOOD

In vitro biology of human myeloid leukemia

(2008) Hector Mayani et al.   LEUKEMIA RESEARCH

Stromal-mediated protection of tyrosine kinase inhibitor-treated BCR-ABL-expressing leukemia cells

(2008) E. Weisberg et al.   MOLECULAR CANCER THERAPEUTICS