Review
Medicine, General & Internal
Suncica Kapor, Juan F. Santibanez
Summary: This review discusses the main features of MDSCs and MSCs in myeloid malignancies, revealing that MDSCs are elevated in numbers and exhibit strong immunosuppressive capabilities, while MSCs not only have immunosuppressive properties but also regulate the growth, apoptosis, and chemotherapy resistance of myeloid leukemia cells.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ita Novita Sari, Ying-Gui Yang, Yoseph Toni Wijaya, Nayoung Jun, Sanghyun Lee, Kwang Seock Kim, Jeevisha Bajaj, Vivian G. Oehler, Soo-Hyun Kim, Soo-Young Choi, Sa-Hee Park, Dong-Wook Kim, Tannishtha Reya, Jaeseok Han, Hyog Young Kwon
Summary: AMD1 plays a crucial role in the progression of myeloid leukemia, and its depletion leads to differentiation of leukemic stem cells and impairs leukemia progression. Pharmacological inhibition of AMD1 can significantly reduce the progression of leukemia cells, offering a new therapeutic approach for the treatment of myeloid leukemia.
Editorial Material
Medicine, Research & Experimental
Jose Maria Lamo-Espinosa, Felipe Prosper, Juan F. Blanco, Fermin Sanchez-Guijo, Mercedes Alberca, Veronica Garcia, Margarita Gonzalez-Vallinas, Javier Garcia-Sancho
Summary: The study found that using 10, 40, and 100 million autologous cells for knee osteoarthritis treatment provided similar and long-lasting results, providing strong evidence for the efficacy of single-cell injection therapy.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Cell Biology
Beverly Brooks, Dominique Ebedes, Ahsan Usmani, Joaquin Vega Gonzales-Portillo, Daniel Gonzales-Portillo, Cesario Borlongan
Summary: Ischemic brain injury is a major cause of death worldwide with limited treatment options. Stem cell-based regenerative therapies, specifically mesenchymal stromal cells, show promise in stroke treatment, but further research is needed to fully understand their cell function.
Article
Pharmacology & Pharmacy
Paula Laranjeira, Monia Pedrosa, Catia Duarte, Susana Pedreiro, Brigida Antunes, Tania Ribeiro, Francisco dos Santos, Antonio Martinho, Margarida Fardilha, M. Rosario Domingues, Manuel Abecasis, Jose Antonio Pereira da Silva, Artur Paiva
Summary: This study found that BM-MSC effectively reduced TNF-alpha, CD83, CCR7, and MIP-1 beta protein levels in mDCs and all monocyte subsets in RA patients. BM-MSC exhibited a suppressive action over antigen-presenting cells from RA patients, potentially affecting their ability to stimulate the immune adaptive response at different levels.
Review
Pharmacology & Pharmacy
Katerina E. E. Miari, Mark T. S. Williams
Summary: The bone marrow plays a vital role in adult hematopoiesis, but abnormalities in stromal elements such as fibroblasts and MSCs can lead to blood malignancies like AML. This review emphasizes the significance of bone marrow fibroblasts and MSCs in both health and AML, and their impact on patient prognosis. The review also discusses how stromal elements contribute to therapy resistance by creating an immunosuppressive microenvironment, making it challenging to target the bone marrow stroma for improved AML outcomes.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Clinical Neurology
Amara Larpthaveesarp, Praneeti Pathipati, Samuel Ostrin, Anthony Rajah, Donna Ferriero, Fernando F. Gonzalez
Summary: The study found that both single-dose MSC/EPO and multidose systemic EPO can improve long-term neurobehavioral outcomes even when administered after a delay, with EPO showing the most overall effectiveness.
Review
Cell Biology
Meryem Ouzin, Gesine Kogler
Summary: Mesenchymal stromal cells are widely used in regenerative medicine and translational research, but their heterogeneity and the lack of understanding of their molecular mechanisms pose challenges for clinical applications. This review focuses on the heterogeneity aspects related to tissue source and developmental origin, emphasizing the importance of generating homogeneous products for clinical use.
Review
Oncology
Isabella Maria Mayer, Andrea Hoelbl-Kovacic, Veronika Sexl, Eszter Doma
Summary: Transplantation of adult hematopoietic stem cells is crucial for treating hematological disorders, while leukemic stem cells play a significant role in disease initiation and relapse. Understanding methods to maintain and expand hematopoietic cells, as well as isolate and enrich hematopoietic stem cells and leukemic stem cells, is essential for developing therapeutic strategies.
Article
Biochemistry & Molecular Biology
Natalie Vallant, Bynvant Sandhu, Karim Hamaoui, Maria Prendecki, Charles Pusey, Vassilios Papalois
Summary: This study compared the paracrine effects of MSC supernatants from different age and genotype sources on macrophages, revealing distinct patterns of inflammatory marker expression. These findings are crucial for preclinical and clinical studies utilizing MSCs for inflammation and allo-response mitigation in transplant patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Lihui Peng, Julien E. Gautrot
Summary: This study demonstrates that bioemulsions formed of microdroplets stabilized by protein nanosheets are well-suited for scale up of adherent stem cells like MSCs. Phenotyping of MSCs cultured on these bioemulsions, solid microcarriers, and classic 2D tissue culture polystyrene over multiple passages shows comparable phenotypes, although stemness is lost at late passages. This validates the use of bioemulsions for long term expansion of adherent stem cells and opens possibilities for novel 3D bioreactors based on microdroplet microcarriers.
MATERIALS TODAY BIO
(2021)
Review
Immunology
Ayman F. Refaie, Batoul L. Elbassiouny, Malgorzata Kloc, Omaima M. Sabek, Sherry M. Khater, Amani M. Ismail, Rania H. Mohamed, Mohamed A. Ghoneim
Summary: MSC-based therapy for T1DM shows promise, but there are conflicting views on their immunological profile. Research focuses on understanding the mechanisms of their immunomodulatory functions and exploring ways to enhance their effects.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Oncology
Meixi Peng, Yongxiu Huang, Ling Zhang, Xueya Zhao, Yu Hou
Summary: Acute myeloid leukemia (AML) is a hematologic malignancy with poor prognosis, characterized by various cytogenetic and molecular abnormalities. Current treatments often fail to eliminate leukemic stem cells (LSCs), which exhibit a unique metabolic profile dependent on oxidative phosphorylation (OXPHOS). Understanding the regulation of OXPHOS in LSCs may lead to effective clinical therapies in AML.
FRONTIERS IN ONCOLOGY
(2022)
Article
Pharmacology & Pharmacy
Fahui Li, Congying Gao, Xueming Li, Jiangyun Wang, Yao Zhao, Yu Ke, Ying Liu, Hong-Min Liu, Zhenbo Hu, Liuya Wei, Zhe-Sheng Chen
Summary: The natural compound Jiyuan oridonin A (JOA) has been found to overcome the differentiation blockade in AML cells and leukemic stem-like cells, inhibiting their proliferation and offering a novel therapeutic strategy for AML.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Cell Biology
Chenghai Li, Bin Wang
Summary: Fibrosis refers to the connective tissue deposition and stiffness usually caused by injury, with tissue-resident mesenchymal cells playing a significant role in fibrogenic processes. MSCs may be a potential therapeutic option for fibrosis resolution, but their functional benefits in translational applications need to be carefully balanced with their potential risks.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Hematology
Stefan Lundh, Sayantan Maji, J. Joseph Melenhorst
Summary: CAR T-cell therapy, as a rapidly emerging treatment option in oncology, has shown significant efficacy and potential for cure in certain relapsed/refractory patients with hematologic malignancy. However, there are various barriers for widespread adoption of CAR T therapy, including cellular manufacturing limitations, adverse events post-infusion, and host rejection of non-human CARs. Future clinical trials need to address unique mechanisms of relapse in different hematologic diseases to enhance the efficacy of CAR T treatment.
INTERNATIONAL JOURNAL OF HEMATOLOGY
(2021)
Article
Orthopedics
Philip Basile, Samantha A. Miner, Jordan W. Crafton, Bryon McKenna
Summary: This article presents a hybrid technique for total ankle arthroplasty using a stemmed intramedullary tibial component and a chamfer-cut talar component. The study shows that this technique is reproducible, accurate, and safe, with promising preliminary outcomes.
JOURNAL OF FOOT & ANKLE SURGERY
(2022)
Article
Multidisciplinary Sciences
J. Joseph Melenhorst, Gregory M. Chen, Meng Wang, David L. Porter, Changya Chen, McKensie A. Collins, Peng Gao, Shovik Bandyopadhyay, Hongxing Sun, Ziran Zhao, Stefan Lundh, Iulian Pruteanu-Malinici, Christopher L. Nobles, Sayantan Maji, Noelle Frey, Saar Gill, Lifeng Tian, Irina Kulikovskaya, Minnal Gupta, David E. Ambrose, Megan M. Davis, Joseph A. Fraietta, Jennifer L. Brogdon, Regina M. Young, Anne Chew, Bruce L. Levine, Donald L. Siegel, Cecile Alanio, E. John Wherry, Frederic D. Bushman, Simon F. Lacey, Kai Tan, Carl H. June
Summary: CAR T cells redirected to target CD19 demonstrated long-lasting potential and clonal stability in two patients with chronic lymphocytic leukaemia. Highly activated CD4(+) cells emerged and dominated the CAR T cell population at later time points. These unexpected CAR T cell populations provide novel insights into anti-cancer response and long-term remission in leukaemia.
Article
Hematology
David J. Young, Xing Fan, Emma M. Groarke, Bhavisha Patel, Ronan Desmond, Thomas Winkler, Andre Larochelle, Katherine R. Calvo, Neal S. Young, Cynthia E. Dunbar
Summary: EPAG treatment for aplastic anemia and immune thrombocytopenia can result in iron depletion over time. This study found that duration of EPAG exposure correlated with reduction in ferritin levels and showed faster iron clearance compared to historical responders. Baseline ferritin and duration of therapy were associated with risk of iron depletion, but did not affect treatment response or relapse risk.
AMERICAN JOURNAL OF HEMATOLOGY
(2022)
Editorial Material
Hematology
Arnon P. Kater, J. Joseph Melenhorst
Summary: This study summarizes a phase 1/2 dose-escalation chimeric antigen receptor T (CART) trial for patients with chronic lymphocytic leukemia (CLL). The CART19 cells treatment showed promising results, with most patients experiencing cytokine release syndrome and neurotoxicity, but achieving long-lasting complete responses and undetectable minimal residual disease in a subset of patients.
Editorial Material
Cell Biology
Gregory M. Chen, Jan Joseph Melenhorst, Kai Tan
Summary: CAR T cell therapies targeting CD19 and CD22 have shown success in treating B cell cancers, but not in non-B cell cancers. The interaction between CAR T cells and normal B cells may play a crucial role in determining clinical CAR T cell responses.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Review
Orthopedics
Samantha A. Miner, John A. Martucci, Stephen A. Brigido, Lawrence DiDomenico
Summary: Although periprosthetic joint infection (PJI) is not common after total ankle arthroplasty (TAA), it poses a significant risk to implant failure. This systematic review aimed to evaluate the time to revision surgery after PJI in TAA patients. A total of 12 articles studying 3040 implants were included, and the average time to revision due to PJI was found to be 30.7 months. It is suggested to modify the current PJI classification to include early chronic and late chronic subgroups for better diagnosis and treatment of PJIs.
JOURNAL OF FOOT & ANKLE SURGERY
(2023)
Editorial Material
Oncology
Ziran Zhao, Jan Joseph Melenhorst
CANCER IMMUNOLOGY RESEARCH
(2022)
Article
Orthopedics
Samantha A. Miner, Philip Basile, Jeremy Cook, Emily Cook, John Constantino
Summary: Total ankle arthroplasty (TAA) is an effective treatment for end-stage ankle arthritis, but concerns have been raised about early component loosening. A retrospective study compared the short-term outcomes of the tibial component between a standard TAA system and a hybrid technique. The rate of tibial component subsidence was low in both groups, and further studies are needed to explore factors that may contribute to early tibial component subsidence and implant failure.
JOURNAL OF FOOT & ANKLE SURGERY
(2023)
Correction
Multidisciplinary Sciences
J. Joseph Melenhorst, Gregory M. M. Chen, Meng Wang, David L. L. Porter, Changya Chen, McKensie A. A. Collins, Peng Gao, Shovik Bandyopadhyay, Hongxing Sun, Ziran Zhao, Stefan Lundh, Iulian Pruteanu-Malinici, Christopher L. L. Nobles, Sayantan Maji, Noelle V. V. Frey, Saar I. I. Gill, Alison W. W. Loren, Lifeng Tian, Irina Kulikovskaya, Minnal Gupta, David E. E. Ambrose, Megan M. M. Davis, Joseph A. A. Fraietta, Jennifer L. L. Brogdon, Regina M. M. Young, Anne Chew, Bruce L. L. Levine, Donald L. L. Siegel, Cecile Alanio, E. John Wherry, Frederic D. D. Bushman, Simon F. F. Lacey, Kai Tan, Carl H. H. June
Review
Cell Biology
Jianyi Ding, Yongqin Li, Andre Larochelle
Summary: The ability to produce human hematopoietic stem cells (HSCs) in the laboratory holds great potential for cellular therapy of blood diseases. However, current differentiation protocols for generating HSCs from pluripotent stem cells (PSCs) lack clinical relevance and result in limited engraftment and differentiation capabilities. This review discusses how intrinsic cues and extrinsic environmental inputs have been integrated into PSC differentiation protocols to enhance the emergence of definitive hematopoiesis, and explores the role of genomics in advancing this field.
Article
Cell & Tissue Engineering
Yongqin Li, Jianyi Ding, Daisuke Araki, Jizhong Zou, Andre Larochelle
Summary: Researchers optimized the differentiation of induced pluripotent stem cells into hematopoietic stem and progenitor cells by modulating specific signaling pathways, resulting in increased self-renewal and multilineage differentiation potential.
Article
Hematology
Ziran Zhao, Celine Gregoire, Beatriz Oliveira, Kunho Chung, Jan Joseph Melenhorst
Summary: Chimeric antigen receptor (CAR) T-cell therapies have revolutionized the treatment of blood cancers by endowing T cells with antibody-like target cell recognition and lysis abilities. In chronic B lymphocytic leukemia (CLL), CAR T-cells have shown efficacy, but sustained remission remains a challenge. This article discusses the current treatment landscape in CLL, the role of CAR T-cells, and the improvements made through translational medicine.
SEMINARS IN HEMATOLOGY
(2023)
Article
Cell Biology
Meraj H. Khan, Jan Joseph Melenhorst
Summary: This study reveals that the positively charged patches on the surface of CAR antigen-binding domain regulate CAR tonic signaling, offering a direction for further optimization of CAR.
Article
Oncology
Kavita M. Dhodapkar, Adam D. Cohen, Akhilesh Kaushal, Alfred L. Garfall, Renee Julia Manalo, Allison R. Carr, Samuel S. McCachren III, Edward A. Stadtmauer, Simon F. Lacey, J. Joseph Melenhorst, Carl H. June, Michael C. Milone, Madhav Dhodapkar
Summary: This study combined multiple approaches to study the tumor/immune cells in the tumor microenvironment of myeloma patients before and after BCMA CAR T therapy. The findings suggest that lower diversity of T-cell receptor repertoire, presence of hyperexpanded clones and exhausted phenotype, as well as the presence of specific cells in the bone marrow are associated with shorter progression-free survival following therapy. Conversely, longer progression-free survival is associated with an increased proportion of certain cells and the emergence of cells expressing marrow-residence genes. Tumor recurrence is associated with the emergence of new dominant clones.
BLOOD CANCER DISCOVERY
(2022)