Article
Cell Biology
Qian-Ru He, Meng Cong, Fan-Hui Yu, Yu-Hua Ji, Shu Yu, Hai-Yan Shi, Fei Ding
Summary: Peripheral nerve fibroblasts play a critical role in nerve development and regeneration by promoting motoneuron neurite outgrowth. These fibroblasts exhibit different patterns of gene expression compared to cardiac fibroblasts.
NEURAL REGENERATION RESEARCH
(2022)
Review
Medicine, Research & Experimental
Stefan M. Gysler, Ronny Drapkin
Summary: Recent cancer research has shifted focus from abnormal cell division to the complex interactions between cancer and noncancer cells in the tumor microenvironment. The recruitment of peripheral nerves to the TME by tumors has been linked to enhanced tumor growth and aggressive tumor behavior, suggesting a potential role for the peripheral nervous system in cancer development. Emerging evidence also suggests that exosomes may mediate neural regulation of the TME through cell-cell communication.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Neurosciences
Barbara Rangel da Silva, Ricardo A. de Melo Reis, Victor Tulio Ribeiro-Resende
Summary: The vascular and nervous systems have similarities and play complex roles in providing oxygen and nutrients to cells. They are both highly branched networks that often grow close to each other during development. Recent studies have focused on their relationship in peripheral nervous system regeneration, where endothelial cells guide the formation of Bungner bands that support axonal regeneration.
MOLECULAR NEUROBIOLOGY
(2023)
Review
Cell Biology
Kamilla Faritovna Idrisova, Alina Kazymovna Zeinalova, Galina Andreevna Masgutova, Alexey Andreevich Bogov, Cinzia Allegrucci, Valeriia Yurievna Syromiatnikova, Ilnur Ildusovich Salafutdinov, Ekaterna Evgenievna Garanina, Dina Ivanovna Andreeva, Adilet Abdullaatovich Kadyrov, Albert Anatolevich Rizvanov, Ruslan Faridovich Masgutov
Summary: The limited ability of peripheral nerves to regenerate after injury can lead to poor motor function and permanent disability. Gene therapy and cell therapy-based drugs that increase nerve growth factors can compensate for this limitation. Fibroblast growth factor 2 and vascular endothelial growth factor have positive effects on angiogenesis and neural cell growth, making them important for nerve regeneration.
NEURAL REGENERATION RESEARCH
(2022)
Article
Multidisciplinary Sciences
Patrick Doemer, Janine Kayal, Ulrike Janssen-Bienhold, Bettina Kewitz, Thomas Kretschmer, Christian Heinen
Summary: A novel and efficient protocol for isolating endothelial cells from human peripheral nerves was established, achieving >95% purity. The isolated ECs expressed highly specific EC marker proteins and showed functional properties, demonstrating potential applications in human blood-nerve-barrier research and peripheral nerve regeneration.
SCIENTIFIC REPORTS
(2021)
Article
Veterinary Sciences
Rebecca M. Harman, Katherine A. Churchill, Sonia Parmar, Gerlinde R. van de Walle
Summary: This study reports the isolation and characterization of chicken PBMSCs from peripheral blood, which can secrete bioactive factors with antimicrobial and regenerative properties, providing a novel source of cell-based therapies for the poultry industry.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Xinyi Tang, Bihui Zhu, Mei Tian, Ruiqian Guo, Songya Huang, Yuanjiao Tang, Li Qiu
Summary: The study used SWE technology to measure the SWV of peripheral nerves in healthy individuals and found significant differences in SWV between different nerve sites and different peripheral nerves. There were no significant differences in SWV between bilateral median nerves. SWV of peripheral nerves was associated with gender, but not with age or BMI.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Krzysztof Gomulka, Maciej Tota, Kacper Brzdak
Summary: This study aimed to investigate the expression of CD11b activation marker on peripheral eosinophils in asthmatics with different degrees of airway narrowing. The results showed that CD11b was slightly presented on unstimulated eosinophils in asthmatics, and stimulation with VEGF enhanced their activity and induced CD11b expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Matteo Gallazzi, Denisa Baci, Lorenzo Mortara, Annalisa Bosi, Giuseppe Buono, Angelo Naselli, Andrea Guarneri, Federico Deho, Paolo Capogrosso, Adriana Albini, Douglas M. Noonan, Antonino Bruno
Summary: This study characterized the phenotype and function of peripheral blood NK cells in prostate cancer patients, finding that they promote inflammatory angiogenesis in endothelial cells and modulate monocytes and macrophages towards an M2-like phenotype. These findings suggest a potential use of peripheral blood NK cell profiling in prostate cancer patients.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hayley McMillan, Fionnuala T. Lundy, Orla M. Dunne, Banan Al-Natour, Charlotte Jeanneau, Imad About, Tim M. Curtis, Ikhlas El Karim
Summary: This study investigates the role of MrgprX1 in nociception by examining its interaction with the pain receptor TRPA1. Results show that MrgprX1 activates TRPA1 and sensitizes it to agonist stimulation via Protein Kinase C, suggesting an important role for this receptor in pain modulation.
Article
Neurosciences
Yi-jun Liu, Xiao-feng Chen, Li-ping Zhou, Feng Rao, Dian-ying Zhang, Yan-hua Wang
Summary: This study aimed to analyze the effect and mechanism of Wnt5a-loaded fibrin hydrogel on a 10-mm rat sciatic nerve defect. Results showed that Wnt5a could promote Schwann cell proliferation and the expression of various neurotrophic factors, and the Wnt5a/Fn group outperformed the control groups in terms of nerve regeneration and functional recovery 12 weeks after the operation. The Wnt5a/Fn nerve conduit may promote peripheral nerve defect regeneration by facilitating multiple neurotrophin secretion, combining vascularization and neurotrophic growth cues.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Larissa Schawe, Ben Raude, Jan Christoph Carstens, Irene Hinterseher, Raphael Donatus Hein, Safwan Omran, Gilles Berger, Nina A. Hering, Matthias Buerger, Andreas Greiner, Jan Paul Frese
Summary: The study investigated the role of vascular endothelial growth factor (VEGF) in ischemic and non-ischemic skeletal muscle in patients with peripheral arterial disease (PAD). The results showed that preoperative VEGF levels were significantly higher in ischemic muscles compared to controls, and postoperative values decreased significantly. There was no significant change in VEGF levels in the non-ischemic muscle. This suggests impaired angiogenesis mechanisms in PAD patients.
Review
Nutrition & Dietetics
Daniel J. York, Anne L. Smazal, Daniel T. Robinson, Isabelle G. De Plaen
Summary: Human milk contains growth factors that play a crucial role in preventing necrotizing enterocolitis, especially in preterm infants. These growth factors have significant effects on intestinal growth, barrier function, microvascular development, and immunological maturation.
Article
Chemistry, Multidisciplinary
Yizhu Shan, Lingling Xu, Xi Cui, Engui Wang, Fengying Jiang, Jiaxuan Li, Han Ouyang, Tailang Yin, Hongqing Feng, Dan Luo, Yan Zhang, Zhou Li
Summary: A responsive cascade drug delivery scaffold (RCDDS) has been developed to dynamically adjust drug release kinetics based on the patient's condition, promoting the treatment of peripheral nerve injury.
MATERIALS HORIZONS
(2023)
Article
Anatomy & Morphology
Diane Tomalty, Olivia Giovannetti, Leah Velikonja, Jasica Munday, Martin Kaufmann, Natasha Iaboni, Amoon Jamzad, Rachel Rubino, Gabor Fichtinger, Parvin Mousavi, Christopher J. B. Nicol, John F. F. Rudan, Michael A. A. Adams
Summary: Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) is an effective tool for metabolomic profiling of peripheral nerves, allowing for discrimination of molecular characteristics and differentiation of nerve subtypes.
JOURNAL OF ANATOMY
(2023)
Article
Mathematics, Interdisciplinary Applications
David Kaplan, Jianshen Chen, Sinan Yavuz, Weicong Lyu
Summary: The purpose of this paper is to demonstrate and evaluate the use of Bayesian dynamic borrowing in large-scale educational assessments. The results show that Bayesian dynamic borrowing performs well in cases with heterogeneous historical data compared to other methods, while it performs similarly to other methods in cases of homogeneous data.
Article
Neurosciences
Dustin M. Snapper, Bianca Reginauld, Volha Liaudanskaya, Vincent Fitzpatrick, Yeonho Kim, Irene Georgakoudi, David L. Kaplan, Aviva J. Symes
Summary: This study developed a novel approach to studying traumatic brain injury caused by primary blast and utilized a bioengineered 3D brain-like human tissue culture system to simulate blast wave effects. The findings showed cellular injury, axonal varicosities, and the release of known biomarkers after blast.
JOURNAL OF NEUROSCIENCE RESEARCH
(2023)
Review
Pharmacology & Pharmacy
Constancio Gonzalez-Obeso, Emily Jane Hartzell, Ryan Albert Scheel, David L. Kaplan
Summary: Effective drug delivery is crucial for successful medical treatment. Silks and recombinant silks have emerged as preferred polymeric drug delivery systems due to their unique properties and tailored physicochemical characteristics.
ADVANCED DRUG DELIVERY REVIEWS
(2023)
Article
Materials Science, Multidisciplinary
Shengfei Zhou, Kai Jin, Talia Khan, Zaira Martin-Moldes, David L. Kaplan, Markus J. Buehler
Summary: More complex bioadhesives were investigated, and the physical properties of the adhesive were improved through cold denaturation and swelling. This resulted in enhanced mechanical performance and water resistance of wood composites.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Multidisciplinary Sciences
Maobin Xie, Liming Lian, Xuan Mu, Zeyu Luo, Carlos Ezio Garciamendez-Mijares, Zhenrui Zhang, Arturo Lopez, Jennifer Manriquez, Xiao Kuang, Junqi Wu, Jugal Kishore Sahoo, Federico Zertuche Gonzalez, Gang Li, Guosheng Tang, Sushila Maharjan, Jie Guo, David L. Kaplan, Yu Shrike Zhang
Summary: This study demonstrates the rapid volumetric printing of unmodified silk sericin and silk fibroin inks, which exhibit shape-memory and tuneable mechanical properties. The inks are cytocompatible, providing expanded bioink libraries for volumetric additive manufacturing and offering potential for diverse biomedical applications.
NATURE COMMUNICATIONS
(2023)
Article
Materials Science, Multidisciplinary
Jaewon Choi, Jugal Kishore Sahoo, Onur Hasturk, Thomas Falcucci, Ya Yao, David L. Kaplan
Summary: This study presents a new strategy to fabricate silk protein-based aerosols and silk fibers instantaneously in situ using a spray device, without the need for complicated and costly advanced manufacturing techniques. The key to success lies in the conformational transition of silk fibroin from random coil to beta-sheet before spraying, achieved by mixing silk and polyethylene glycol solutions in the spray device. The system utilizes green solvents and shear and elongational flow to provide a portable and user-friendly method for drug delivery carriers and surface coatings.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Nanoscience & Nanotechnology
Jugal Kishore Sahoo, Dawei Xu, Thomas Falcucci, Jaewon Choi, Onur Hasturk, Douglas S. Clark, David L. Kaplan
Summary: Protein-based hydrogel biomaterials offer a versatile platform for various biological applications, with the ability to encapsulate and stabilize biomolecules. In this study, silk fibroin and gamma-prefoldin chaperone protein were used to create composite hydrogels with different protein ratios, using enzyme-assisted cross-linking. The secondary structure, dityrosine bond formation, and mechanical properties were evaluated. The findings provide insights for the rational design of composite thermostable hydrogel biomaterials, allowing for protection and retention of bioactivity.
ACS APPLIED BIO MATERIALS
(2023)
Article
Engineering, Biomedical
Andrew J. Stout, Miriam L. Rittenberg, Michelle Shub, Michael K. Saad, Addison B. Mirliani, James Dolgin, David L. Kaplan
Summary: The development of cost-effective serum-free media is crucial for the economic viability of cultured meat. This study successfully replaced recombinant albumin, which is a high-cost component, with rapeseed protein isolate in a serum-free medium called Beefy-R. The new medium showed improved growth and maintained cell phenotype and myogenicity of bovine satellite cells, offering a sustainable and low-cost alternative for muscle stem cell culture in cultured meat production.
Review
Biotechnology & Applied Microbiology
Ying Pei, Wen Yang, Keyong Tang, David L. Kaplan
Summary: Collagen presents a hierarchical multilevel structure that achieves an ideal balance of mechanical strength and toughness. Isolation of mesostructured aggregates of collagen from biological tissues enables the construction of new collagen materials with ideal mechanical performance. The use of these mesostructural components has shown promise in various applications.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Chemistry, Applied
Qiongling Chen, Jinchuang Zhang, Yujie Zhang, Haodong Liu, Tongqing Li, Qiang Wang, David L. Kaplan
Summary: This study investigated the interactions between pea protein and fatty acids as well as the effect on protein conformational changes during high-moisture extrusion processing. The results showed that fatty acids induced the exposure of hydrophobic groups, weakened hydrogen bonds, and affected the aggregation of protein subunits. Unsaturated fatty acids limited protein chain refolding and covalent interactions. Additionally, stearic acid promoted the formation of fibrous structures.
Article
Pharmacology & Pharmacy
Marta G. Fuster, Mercedes G. Montalban, Imane Moulefera, Gloria Villora, David L. Kaplan
Summary: In this study, folic acid-conjugated silk nanoparticles were used to deliver the anticancer drug ibrutinib (IB) to cancer cells with folate receptor overexpression. The study confirmed that the functionalized nanoparticles were internalized by cancer cells, mediated by folate receptors. This suggests that the developed nanocarrier can enhance IB uptake in cancer cells and be used for drug targeting applications.
Review
Chemistry, Multidisciplinary
Jugal Kishore Sahoo, Onur Hasturk, Thomas Falcucci, David L. Kaplan
Summary: Silk fibroin is widely used in various medical fields such as tissue engineering, regenerative medicine, drug delivery, and medical devices. Advancements in silk chemistry and biomaterial designs have resulted in exciting tools for creating new silk-based materials and technologies. Selective chemistries can enhance or modify the properties of silk to address challenges in medically relevant materials.
NATURE REVIEWS CHEMISTRY
(2023)
Article
Green & Sustainable Science & Technology
Kirsten R. Trinidad, Reina Ashizawa, Amin Nikkhah, Cameron Semper, Christian Casolaro, David L. Kaplan, Alexei Savchenko, Nicole Tichenor Blackstone
Summary: Growth factors are critical components in cultivated meat production, but their environmental impacts have not been accurately quantified in current assessments. This study conducted a life cycle assessment to evaluate the environmental impacts of recombinant growth factor production for cultivated meat applications. The results show that even in small quantities, recombinant growth factors can have significant environmental impacts. Future research should explore sustainable production methods or alternative options with lower environmental impacts.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Materials Science, Biomaterials
Gang Xu, Liying Xiao, Peng Guo, Yuanyuan Wang, Shiyu Ke, Guozhong Lyu, Xiangsheng Ding, Qiang Lu, David L. Kaplan
Summary: The study developed an assembly strategy to regulate multiple cues in the same scaffold. The results showed that the multiple cues in the scaffold could promote vascularization and accelerate wound healing.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Engineering, Electrical & Electronic
Atul Sharma, Cihan Asci, Ruben Del-Rio-Ruiz, Kirsten Trinidad, Nafize Ishtiaque Hossain, David L. Kaplan, Sameer Sonkusale
Summary: A portable and scalable multiplexed thread-based sensing microprobe is introduced for continuous monitoring of pH and ammonium ions during the biofermentation process. The microprobe consists of a 3D-printed shell to hold separate working and reference electrode threads, allowing for continuous flow during monitoring. The functionality of the microprobe is evaluated in a simulated microreactor resembling a fermentation environment, showing promising dynamic range for pH and ammonium ions in bioreactor monitoring.
IEEE SENSORS LETTERS
(2023)
