Dynamic modulation of the lipid translocation groove generates a conductive ion channel in Ca2+-bound nhTMEM16

Lifting the veils on TMEM16E function

(2019) Anna Boccaccio et al.   Channels

Structural basis of Ca2+-dependent activation and lipid transport by a TMEM16 scramblase

(2019) Maria E Falzone et al.   eLife

An inner activation gate controls TMEM16F phospholipid scrambling

(2019) Trieu Le et al.   Nature Communications

Cryo-EM structures and functional characterization of the murine lipid scramblase TMEM16F

(2019) Carolina Alvadia et al.   eLife

Stepwise activation mechanism of the scramblase nhTMEM16 revealed by cryo-EM

(2019) Valeria Kalienkova et al.   eLife

Cryo-EM Studies of TMEM16F Calcium-Activated Ion Channel Suggest Features Important for Lipid Scrambling

(2019) Shengjie Feng et al.   Cell Reports

The structural basis of lipid scrambling and inactivation in the endoplasmic reticulum scramblase TMEM16K

(2019) Simon R. Bushell et al.   Nature Communications

How structural elements evolving from bacterial to human SLC6 transporters enabled new functional properties

(2018) Asghar M. Razavi et al.   BMC BIOLOGY

Known structures and unknown mechanisms of TMEM16 scramblases and channels

(2018) Maria E. Falzone et al.   JOURNAL OF GENERAL PHYSIOLOGY

Out-of-the-groove transport of lipids by TMEM16 and GPCR scramblases

(2018) Mattia Malvezzi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mechanisms of Lipid Scrambling by the G Protein-Coupled Receptor Opsin

(2018) Giulia Morra et al.   STRUCTURE

Gating mechanism of the extracellular entry to the lipid pathway in a TMEM16 scramblase

(2018) Byoung-Cheol Lee et al.   Nature Communications

New tools for automated high-resolution cryo-EM structure determination in RELION-3

(2018) Jasenko Zivanov et al.   eLife

Calcium-dependent electrostatic control of anion access to the pore of the calcium-activated chloride channel TMEM16A

(2018) Andy KM Lam et al.   eLife

A Bayesian approach to beam-induced motion correction in cryo-EM single-particle analysis

(2018) Jasenko Zivanov et al.   IUCrJ

Activation mechanism of the calcium-activated chloride channel TMEM16A revealed by cryo-EM

(2017) Cristina Paulino et al.   NATURE

Cryo-EM structures of the TMEM16A calcium-activated chloride channel

(2017) Shangyu Dang et al.   NATURE

Characterization of the scrambling domain of the TMEM16 family

(2017) Sayuri Gyobu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural basis for anion conduction in the calcium-activated chloride channel TMEM16A

(2017) Cristina Paulino et al.   eLife

Lipids and ions traverse the membrane by the same physical pathway in the nhTMEM16 scramblase

(2017) Tao Jiang et al.   eLife

A Markov State-based Quantitative Kinetic Model of Sodium Release from the Dopamine Transporter

(2017) Asghar M. Razavi et al.   Scientific Reports

The nhTMEM16 Scramblase Is Also a Nonselective Ion Channel

(2016) Byoung-Cheol Lee et al.   BIOPHYSICAL JOURNAL

A Pore Idea: the ion conduction pathway of TMEM16/ANO proteins is composed partly of lipid

(2016) Jarred M. Whitlock et al.   PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY

Atomistic insight into lipid translocation by a TMEM16 scramblase

(2016) Neville P. Bethel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CHARMM-GUI Martini Maker for Coarse-Grained Simulations with the Martini Force Field

(2015) Yifei Qi et al.   Journal of Chemical Theory and Computation

TMEM16 Proteins: Unknown Structure and Confusing Functions

(2015) Alessandra Picollo et al.   JOURNAL OF MOLECULAR BIOLOGY

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

EMRinger: side chain–directed model and map validation for 3D cryo-electron microscopy

(2015) Benjamin A Barad et al.   NATURE METHODS

MemProtMD: Automated Insertion of Membrane Protein Structures into Explicit Lipid Membranes

(2015) Phillip J. Stansfeld et al.   STRUCTURE

Identification of a lipid scrambling domain in ANO6/TMEM16F

(2015) Kuai Yu et al.   eLife

CHARMM-GUIMembrane Buildertoward realistic biological membrane simulations

(2014) Emilia L. Wu et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

X-ray structure of a calcium-activated TMEM16 lipid scramblase

(2014) Janine D. Brunner et al.   NATURE

Identification of slow molecular order parameters for Markov model construction

(2013) Guillermo Pérez-Hernández et al.   JOURNAL OF CHEMICAL PHYSICS

Simulations of Anionic Lipid Membranes: Development of Interaction-Specific Ion Parameters and Validation Using NMR Data

(2013) Richard M. Venable et al.   JOURNAL OF PHYSICAL CHEMISTRY B

One number does not fit all: Mapping local variations in resolution in cryo-EM reconstructions

(2013) Giovanni Cardone et al.   JOURNAL OF STRUCTURAL BIOLOGY

Ca2+-dependent phospholipid scrambling by a reconstituted TMEM16 ion channel

(2013) Mattia Malvezzi et al.   Nature Communications

Inactivation of anoctamin-6/Tmem16f, a regulator of phosphatidylserine scrambling in osteoblasts, leads to decreased mineral deposition in skeletal tissues

(2012) Harald WA Ehlen et al.   JOURNAL OF BONE AND MINERAL RESEARCH

Optimization of the Additive CHARMM All-Atom Protein Force Field Targeting Improved Sampling of the Backbone ϕ, ψ and Side-Chain χ1 and χ2 Dihedral Angles

(2012) Robert B. Best et al.   Journal of Chemical Theory and Computation

NIH Image to ImageJ: 25 years of image analysis

(2012) Caroline A Schneider et al.   NATURE METHODS

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Update of the CHARMM All-Atom Additive Force Field for Lipids: Validation on Six Lipid Types

(2010) Jeffery B. Klauda et al.   JOURNAL OF PHYSICAL CHEMISTRY B

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

ACEMD: Accelerating Biomolecular Dynamics in the Microsecond Time Scale

(2009) M. J. Harvey et al.   Journal of Chemical Theory and Computation

CHARMM: The biomolecular simulation program

(2009) B. R. Brooks et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae

(2008) David Drew et al.   Nature Protocols

TMEM16A, A Membrane Protein Associated with Calcium-Dependent Chloride Channel Activity

(2008) A. Caputo et al.   SCIENCE