Efficient CRISPR/Cas9 Disruption of Autoimmune-Associated Genes Reveals Key Signaling Programs in Primary Human T Cells

Reduced expression of phosphatase PTPN2 promotes pathogenic conversion of Tregs in autoimmunity

(2019) Mattias N.D. Svensson et al.   JOURNAL OF CLINICAL INVESTIGATION

Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells

(2018) Akiko Seki et al.   JOURNAL OF EXPERIMENTAL MEDICINE

KEAP1Editing Using CRISPR/Cas9 for Therapeutic NRF2 Activation in Primary Human T Lymphocytes

(2018) Sanjeev Noel et al.   JOURNAL OF IMMUNOLOGY

Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing

(2018) Xin Chen et al.   JOURNAL OF IMMUNOLOGY

Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells

(2018) King L. Hung et al.   MOLECULAR THERAPY

p53 inhibits CRISPR–Cas9 engineering in human pluripotent stem cells

(2018) Robert J. Ihry et al.   NATURE MEDICINE

CRISPR–Cas9 genome editing induces a p53-mediated DNA damage response

(2018) Emma Haapaniemi et al.   NATURE MEDICINE

In vitro–transcribed guide RNAs trigger an innate immune response via the RIG-I pathway

(2018) Beeke Wienert et al.   PLOS BIOLOGY

Efficient homology-directed gene editing by CRISPR/Cas9 in human stem and primary cells using tube electroporation

(2018) Xiaoyun Xu et al.   Scientific Reports

Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function

(2018) Eric Shifrut et al.   CELL

Crispr/Cas Mediated Deletion of PTPN22 in Jurkat T Cells Enhances TCR Signaling and Production of IL-2

(2018) Cara Bray et al.   Frontiers in Immunology

Context-specific effects of genetic variants associated with autoimmune disease

(2017) Iris H. Jonkers et al.   HUMAN MOLECULAR GENETICS

Engineering HIV-Resistant, Anti-HIV Chimeric Antigen Receptor T Cells

(2017) Malika Hale et al.   MOLECULAR THERAPY

In vivo CRISPR screening identifies Ptpn2 as a cancer immunotherapy target

(2017) Robert T. Manguso et al.   NATURE

Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery

(2017) Thomas Gaj et al.   NUCLEIC ACIDS RESEARCH

Genetic regulatory effects modified by immune activation contribute to autoimmune disease associations

(2017) Sarah Kim-Hellmuth et al.   Nature Communications

Extrinsic Protein Tyrosine Phosphatase Non-Receptor 22 Signals Contribute to CD8 T Cell Exhaustion and Promote Persistence of Chronic Lymphocytic Choriomeningitis Virus Infection

(2017) Tatiana Jofra et al.   Frontiers in Immunology

Use of short interfering RNA delivered by cationic liposomes to enable efficient down-regulation of PTPN22 gene in human T lymphocytes

(2017) Valentina Perri et al.   PLoS One

Targeted gene editing restores regulated CD40L function in X-linked hyper-IgM syndrome

(2016) Nicholas Hubbard et al.   BLOOD

High Efficiency CRISPR/Cas9-mediated Gene Editing in Primary Human T-cells Using Mutant Adenoviral E4orf6/E1b55k “Helper” Proteins

(2016) Kamila S Gwiazda et al.   MOLECULAR THERAPY

Autoimmune diseases — connecting risk alleles with molecular traits of the immune system

(2016) Maria Gutierrez-Arcelus et al.   NATURE REVIEWS GENETICS

PTPN22 contributes to exhaustion of T lymphocytes during chronic viral infection

(2016) Christian J. Maine et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes

(2016) C. D. Richardson et al.   Nature Communications

Genetic basis of autoimmunity

(2015) Alexander Marson et al.   JOURNAL OF CLINICAL INVESTIGATION

Genetics of autoimmune diseases: insights from population genetics

(2015) Paula S Ramos et al.   JOURNAL OF HUMAN GENETICS

The Role ofPTPN22Risk Variant in the Development of Autoimmunity: Finding Common Ground between Mouse and Human

(2015) David J. Rawlings et al.   JOURNAL OF IMMUNOLOGY

Sequence-specific activation of the DNA sensor cGAS by Y-form DNA structures as found in primary HIV-1 cDNA

(2015) Anna-Maria Herzner et al.   NATURE IMMUNOLOGY

Heterogeneity of autoimmune diseases: pathophysiologic insights from genetics and implications for new therapies

(2015) Judy H Cho et al.   NATURE MEDICINE

Generation of knock-in primary human T cells using Cas9 ribonucleoproteins

(2015) Kathrin Schumann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient modification of CCR5 in primary human hematopoietic cells using a megaTAL nuclease and AAV donor template

(2015) Blythe D. Sather et al.   Science Translational Medicine

CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool

(2015) Manuel Stemmer et al.   PLoS One

CRISPR-Cas systems for editing, regulating and targeting genomes

(2014) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

The tyrosine phosphatase PTPN22 discriminates weak self peptides from strong agonist TCR signals

(2014) Robert J Salmond et al.   NATURE IMMUNOLOGY

PTPN22: the archetypal non-HLA autoimmunity gene

(2014) Stephanie M. Stanford et al.   Nature Reviews Rheumatology

A disease-associated PTPN22 variant promotes systemic autoimmunity in murine models

(2013) Xuezhi Dai et al.   JOURNAL OF CLINICAL INVESTIGATION

Multiple Autoimmune-Associated Variants Confer Decreased IL-2R Signaling in CD4+CD25hi T Cells of Type 1 Diabetic and Multiple Sclerosis Patients

(2013) Karen Cerosaletti et al.   PLoS One

T cell protein tyrosine phosphatase attenuates T cell signaling to maintain tolerance in mice

(2011) Florian Wiede et al.   JOURNAL OF CLINICAL INVESTIGATION

Unraveling the functional implications of GWAS: how T cell protein tyrosine phosphatase drives autoimmune disease

(2011) Julie Zikherman et al.   JOURNAL OF CLINICAL INVESTIGATION

The autoimmune disease–associated PTPN22 variant promotes calpain-mediated Lyp/Pep degradation associated with lymphocyte and dendritic cell hyperresponsiveness

(2011) Jinyi Zhang et al.   NATURE GENETICS

AAV-mediated gene targeting methods for human cells

(2011) Iram F Khan et al.   Nature Protocols

An autoimmune-associated variant in PTPN2 reveals an impairment of IL-2R signaling in CD4+ T cells

(2010) S A Long et al.   GENES AND IMMUNITY

Deletion of the protein tyrosine phosphatase gene PTPN2 in T-cell acute lymphoblastic leukemia

(2010) Maria Kleppe et al.   NATURE GENETICS

PTPN22 Deficiency Cooperates with the CD45 E613R Allele to Break Tolerance on a Non-Autoimmune Background

(2009) J. Zikherman et al.   JOURNAL OF IMMUNOLOGY