Structural and functional modularity of the U2 snRNP in pre-mRNA splicing

BUD13 Promotes a Type I Interferon Response by Countering Intron Retention in Irf7

(2019) Luke Frankiw et al.   MOLECULAR CELL

Introns are mediators of cell response to starvation

(2019) Julie Parenteau et al.   NATURE

Excised linear introns regulate growth in yeast

(2019) Jeffrey T. Morgan et al.   NATURE

Molecular Mechanisms of pre-mRNA Splicing through Structural Biology of the Spliceosome

(2019) Chuangye Yan et al.   Cold Spring Harbor Perspectives in Biology

Structural Insights into Nuclear pre-mRNA Splicing in Higher Eukaryotes

(2019) Berthold Kastner et al.   Cold Spring Harbor Perspectives in Biology

Structural Basis of Nuclear pre-mRNA Splicing: Lessons from Yeast

(2019) Clemens Plaschka et al.   Cold Spring Harbor Perspectives in Biology

A U2-snRNP–independent role of SF3b in promoting mRNA export

(2019) Ke Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Craving for Introns

(2019) Michela Zaffagni et al.   MOLECULAR CELL

Structures of the Catalytically Activated Yeast Spliceosome Reveal the Mechanism of Branching

(2019) Ruixue Wan et al.   CELL

Dynamic stacking of an expected branch point adenosine in duplexes containing pseudouridine-modified or unmodified U2 snRNA sites

(2019) Scott D. Kennedy et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Cus2 enforces the first ATP-dependent step of splicing by binding to yeast SF3b1 through a UHM-ULM interaction

(2019) Jason Talkish et al.   RNA

Mechanism of 5ʹ splice site transfer for human spliceosome activation

(2019) Clément Charenton et al.   SCIENCE

Cryo-EM Structures of a Group II Intron Reverse Splicing into DNA

(2019) Daniel B. Haack et al.   CELL

Rearrangements within the U6 snRNA Core during the Transition between the Two Catalytic Steps of Splicing

(2019) Katarzyna Eysmont et al.   MOLECULAR CELL

Dynamics of the DEAD-box ATPase Prp5 RecA-like domains provide a conformational switch during spliceosome assembly

(2019) David H Beier et al.   NUCLEIC ACIDS RESEARCH

Disease-Causing Mutations in SF3B1 Alter Splicing by Disrupting Interaction with SUGP1

(2019) Jian Zhang et al.   MOLECULAR CELL

Molecular Mechanism and Evolution of Nuclear Pre-mRNA and Group II Intron Splicing: Insights from Cryo-Electron Microscopy Structures

(2018) Wojciech P. Galej et al.   CHEMICAL REVIEWS

Targeting splicing abnormalities in cancer

(2018) Anant A Agrawal et al.   CURRENT OPINION IN GENETICS & DEVELOPMENT

Most human introns are recognized via multiple and tissue-specific branchpoints

(2018) Jose Mario Bello Pineda et al.   GENES & DEVELOPMENT

The cryo-EM structure of the SF3b spliceosome complex bound to a splicing modulator reveals a pre-mRNA substrate competitive mechanism of action

(2018) Lorenzo I. Finci et al.   GENES & DEVELOPMENT

Stress-induced Pseudouridylation Alters the Structural Equilibrium of Yeast U2 snRNA Stem II

(2018) Clarisse van der Feltz et al.   JOURNAL OF MOLECULAR BIOLOGY

Structural Basis of Splicing Modulation by Antitumor Macrolide Compounds

(2018) Constantin Cretu et al.   MOLECULAR CELL

The life of U6 small nuclear RNA, from cradle to grave

(2018) Allison L. Didychuk et al.   RNA

Functional analysis of Hsh155/SF3b1 interactions with the U2 snRNA/branch site duplex

(2018) Tucker J. Carrocci et al.   RNA

Structures of the fully assembledSaccharomyces cerevisiaespliceosome before activation

(2018) Rui Bai et al.   SCIENCE

Molecular principles underlying dual RNA specificity in the Drosophila SNF protein

(2018) Gert Weber et al.   Nature Communications

Somatic Mutational Landscape of Splicing Factor Genes and Their Functional Consequences across 33 Cancer Types

(2018) Michael Seiler et al.   Cell Reports

RES complex is associated with intron definition and required for zebrafish early embryogenesis

(2018) Juan Pablo Fernandez et al.   PLoS Genetics

Synthetic Lethal and Convergent Biological Effects of Cancer-Associated Spliceosomal Gene Mutations

(2018) Stanley Chun-Wei Lee et al.   CANCER CELL

Prespliceosome structure provides insights into spliceosome assembly and regulation

(2018) Clemens Plaschka et al.   NATURE

Structures of the human pre-catalytic spliceosome and its precursor spliceosome

(2018) Xiechao Zhan et al.   CELL RESEARCH

The pre-mRNA splicing and transcription factor Tat-SF1 is a functional partner of the spliceosome SF3b1 subunit via a U2AF homology motif interface

(2018) Sarah Loerch et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Modeling human RNA spliceosome mutations in the mouse: not all mice were created equal

(2018) Jane Jialu Xu et al.   EXPERIMENTAL HEMATOLOGY

The RES complex is required for efficient transformation of the precatalytic B spliceosome into an activated Bactcomplex

(2017) Penghui Bao et al.   GENES & DEVELOPMENT

Large-scale analysis of branchpoint usage across species and cell lines

(2017) Allison J. Taggart et al.   GENOME RESEARCH

Structure of a pre-catalytic spliceosome

(2017) Clemens Plaschka et al.   NATURE

Structure of a spliceosome remodelled for exon ligation

(2017) Sebastian M. Fica et al.   NATURE

Cryo-EM structure of a human spliceosome activated for step 2 of splicing

(2017) Karl Bertram et al.   NATURE

Splicing and transcription touch base: co-transcriptional spliceosome assembly and function

(2017) Lydia Herzel et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Structural toggle in the RNaseH domain of Prp8 helps balance splicing fidelity and catalytic efficiency

(2017) Megan Mayerle et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p-binding domain

(2017) Anne-Marie M. van Roon et al.   RNA

Postcatalytic spliceosome structure reveals mechanism of 3′–splice site selection

(2017) Max E. Wilkinson et al.   SCIENCE

Structure of the yeast spliceosomal postcatalytic P complex

(2017) Shiheng Liu et al.   SCIENCE

Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A–SF3b complex

(2017) Teng Teng et al.   Nature Communications

Molecular basis of differential 3′ splice site sensitivity to anti-tumor drugs targeting U2 snRNP

(2017) Luisa Vigevani et al.   Nature Communications

Rules and tools to predict the splicing effects of exonic and intronic mutations

(2017) Kinji Ohno et al.   Wiley Interdisciplinary Reviews-RNA

Spliceosomal DEAH-Box ATPases Remodel Pre-mRNA to Activate Alternative Splice Sites

(2016) Daniel R. Semlow et al.   CELL

Pseudouridines in U2 snRNA stimulate the ATPase activity of Prp5 during spliceosome assembly

(2016) Guowei Wu et al.   EMBO JOURNAL

SF3B1/Hsh155 HEAT motif mutations affect interaction with the spliceosomal ATPase Prp5, resulting in altered branch site selectivity in pre-mRNA splicing

(2016) Qing Tang et al.   GENES & DEVELOPMENT

Molecular Architecture of SF3b and Structural Consequences of Its Cancer-Related Mutations

(2016) Constantin Cretu et al.   MOLECULAR CELL

Cryo-EM structure of the spliceosome immediately after branching

(2016) Wojciech P. Galej et al.   NATURE

TheTORsignaling pathway regulates starvation-induced pseudouridylation of yeast U2 snRNA

(2016) Guowei Wu et al.   RNA

Structure–function analysis and genetic interactions of the SmG, SmE, and SmF subunits of the yeast Sm protein ring

(2016) Beate Schwer et al.   RNA

Unmasking the U2AF homology motif family: a bona fide protein–protein interaction motif in disguise

(2016) Sarah Loerch et al.   RNA

Interchangeable SF3B1 inhibitors interfere with pre-mRNA splicing at multiple stages

(2016) Kerstin A. Effenberger et al.   RNA

Structure of a yeast catalytic step I spliceosome at 3.4 A resolution

(2016) R. Wan et al.   SCIENCE

Structure of a yeast step II catalytically activated spliceosome

(2016) Chuangye Yan et al.   SCIENCE

Molecular architecture of the Saccharomyces cerevisiae activated spliceosome

(2016) R. Rauhut et al.   SCIENCE

Structure of a yeast activated spliceosome at 3.5 A resolution

(2016) C. Yan et al.   SCIENCE

A global genetic interaction network maps a wiring diagram of cellular function

(2016) M. Costanzo et al.   SCIENCE

Cancer-associated SF3B1 mutations affect alternative splicing by promoting alternative branchpoint usage

(2016) Samar Alsafadi et al.   Nature Communications

Single molecule analysis reveals reversible and irreversible steps during spliceosome activation

(2016) Aaron A Hoskins et al.   eLife

The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction

(2016) Jean-Baptiste Fourmann et al.   eLife

A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence

(2015) Wen-Wei Liang et al.   GENES & DEVELOPMENT

The NineTeen Complex (NTC) and NTC-associated proteins as targets for spliceosomal ATPase action during pre-mRNA splicing

(2015) Rogerio Alves de Almeida et al.   RNA Biology

Conformational dynamics of stem II of the U2 snRNA

(2015) Margaret L. Rodgers et al.   RNA

Structure–function analysis and genetic interactions of the Yhc1, SmD3, SmB, and Snp1 subunits of yeast U1 snRNP and genetic interactions of SmD3 with U2 snRNP subunit Lea1

(2015) Beate Schwer et al.   RNA

Cancer-Associated SF3B1 Hotspot Mutations Induce Cryptic 3′ Splice Site Selection through Use of a Different Branch Point

(2015) Rachel B. Darman et al.   Cell Reports

Transcriptome Sequencing Reveals Potential Mechanism of Cryptic 3’ Splice Site Selection in SF3B1-mutated Cancers

(2015) Christopher DeBoever et al.   PLoS Computational Biology

Binding Affinity and Cooperativity Control U2B″/snRNA/U2A′ RNP Formation

(2014) Sandra G. Williams et al.   BIOCHEMISTRY

Linkage and Allostery in snRNP Protein/RNA Complexes

(2014) Sandra G. Williams et al.   BIOCHEMISTRY

Stem–loop 4 of U1 snRNA is essential for splicing and interacts with the U2 snRNP-specific SF3A1 protein during spliceosome assembly

(2014) Shalini Sharma et al.   GENES & DEVELOPMENT

A Splicing-Dependent Transcriptional Checkpoint Associated with Prespliceosome Formation

(2014) Keerthi T. Chathoth et al.   MOLECULAR CELL

Core structure of the U6 small nuclear ribonucleoprotein at 1.7-Å resolution

(2014) Eric J Montemayor et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Evidence for a group II intron–like catalytic triplex in the spliceosome

(2014) Sebastian M Fica et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Remodeling of U2-U6 snRNA helix I during pre-mRNA splicing by Prp16 and the NineTeen Complex protein Cwc2

(2014) Rebecca Hogg et al.   NUCLEIC ACIDS RESEARCH

The DExD/H-box ATPase Prp2p destabilizes and proofreads the catalytic RNA core of the spliceosome

(2014) A. M. Wlodaver et al.   RNA

Evolution and Impact of Subclonal Mutations in Chronic Lymphocytic Leukemia

(2013) Dan A. Landau et al.   CELL

Cyclin-Dependent Kinase-Associated Protein Cks2 is Associated with Bladder Cancer Progression

(2013) R Chen et al.   JOURNAL OF INTERNATIONAL MEDICAL RESEARCH

Recurrent mutations at codon 625 of the splicing factor SF3B1 in uveal melanoma

(2013) J William Harbour et al.   NATURE GENETICS

A conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligation

(2013) Matthew J Schellenberg et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicing

(2013) Ramya Krishnan et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5' splice site and branch site

(2013) D. J. Crawford et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Post-transcriptional modifications modulate conformational dynamics in human U2-U6 snRNA complex

(2013) K. S. Karunatilaka et al.   RNA

Alternative Spliceosome Assembly Pathways Revealed by Single-Molecule Fluorescence Microscopy

(2013) Inna Shcherbakova et al.   Cell Reports

Crystal Structure of Prp5p Reveals Interdomain Interactions that Impact Spliceosome Assembly

(2013) Zhi-Min Zhang et al.   Cell Reports

Whole-genome analysis informs breast cancer response to aromatase inhibition

(2012) Matthew J. Ellis et al.   NATURE

Pancreatic cancer genomes reveal aberrations in axon guidance pathway genes

(2012) Andrew V. Biankin et al.   NATURE

Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS

(2012) T. Ohrt et al.   RNA

Staying on message: ensuring fidelity in pre-mRNA splicing

(2012) Daniel R. Semlow et al.   TRENDS IN BIOCHEMICAL SCIENCES

The significant other: splicing by the minor spliceosome

(2012) Janne J. Turunen et al.   Wiley Interdisciplinary Reviews-RNA

Reduced fidelity of branch point recognition and alternative splicing induced by the anti-tumor drug spliceostatin A

(2011) A. Corrionero et al.   GENES & DEVELOPMENT

The anti-tumor drug E7107 reveals an essential role for SF3b in remodeling U2 snRNP to expose the branch point-binding region

(2011) E. G. Folco et al.   GENES & DEVELOPMENT

A U1-U2 snRNP Interaction Network during Intron Definition

(2011) W. Shao et al.   MOLECULAR AND CELLULAR BIOLOGY

Structure of the spliceosomal U4 snRNP core domain and its implication for snRNP biogenesis

(2011) Adelaine K. W. Leung et al.   NATURE

Frequent pathway mutations of splicing machinery in myelodysplasia

(2011) Kenichi Yoshida et al.   NATURE

Identification of SAP155 as the Target of GEX1A (Herboxidiene), an Antitumor Natural Product

(2010) Makoto Hasegawa et al.   ACS Chemical Biology

U2 snRNA is inducibly pseudouridylated at novel sites by Pus7p and snR81 RNP

(2010) Guowei Wu et al.   EMBO JOURNAL

Invariant U2 snRNA Nucleotides Form a Stem Loop to Recognize the Intron Early in Splicing

(2010) Rhonda Perriman et al.   MOLECULAR CELL

Spliceostatin A inhibits spliceosome assembly subsequent to prespliceosome formation

(2010) Gabriel A. Roybal et al.   NUCLEIC ACIDS RESEARCH

Spliceosome discards intermediates via the DEAH box ATPase Prp43p

(2010) R. M. Mayas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural model of the p14/SF3b155{middle dot}branch duplex complex

(2010) M. J. Schellenberg et al.   RNA

Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing

(2010) R. M. Lardelli et al.   RNA

The Spliceosome: Design Principles of a Dynamic RNP Machine

(2009) Markus C. Wahl et al.   CELL

Cwc25 Is a Novel Splicing Factor Required after Prp2 and Yju2 To Facilitate the First Catalytic Reaction

(2009) Y.-F. Chiu et al.   MOLECULAR AND CELLULAR BIOLOGY

Insights into Branch Nucleophile Positioning and Activation from an Orthogonal Pre-mRNA Splicing System in Yeast

(2009) Duncan J. Smith et al.   MOLECULAR CELL

The Evolutionarily Conserved Core Design of the Catalytic Activation Step of the Yeast Spliceosome

(2009) Patrizia Fabrizio et al.   MOLECULAR CELL

Crystal structure of human spliceosomal U1 snRNP at 5.5 Å resolution

(2009) Daniel A. Pomeranz Krummel et al.   NATURE

Evidence that U2/U6 helix I promotes both catalytic steps of pre-mRNA splicing and rearranges in between these steps

(2009) M. A. Mefford et al.   RNA

X-ray Structures of U2 snRNA−Branchpoint Duplexes Containing Conserved Pseudouridines†‡

(2008) Yuan Lin et al.   BIOCHEMISTRY

Human branch point consensus sequence is yUnAy

(2008) Kaiping Gao et al.   NUCLEIC ACIDS RESEARCH

Solution structure of the U2 snRNP protein Rds3p reveals a knotted zinc-finger motif

(2008) A.-M. M. van Roon et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA