Integrating Immunology and Microfluidics for Single Immune Cell Analysis

Protein separation under a microfluidic regime

(2018) I. Rodríguez-Ruiz et al.   ANALYST

Single-Cell RT-PCR in Microfluidic Droplets with Integrated Chemical Lysis

(2018) Samuel C. Kim et al.   ANALYTICAL CHEMISTRY

An immunosuppressive pathway for tumor progression

(2018) Antoine Marçais et al.   NATURE MEDICINE

High-dimensional single-cell analysis predicts response to anti-PD-1 immunotherapy

(2018) Carsten Krieg et al.   NATURE MEDICINE

Mass cytometry analysis of immune cells in the brain

(2018) Ben Korin et al.   Nature Protocols

Immunoengineering with biomaterials for enhanced cancer immunotherapy

(2018) Yu-Qing Xie et al.   Wiley Interdisciplinary Reviews-Nanomedicine and Nanobiotechnology

A microfluidics platform for combinatorial drug screening on cancer biopsies

(2018) Federica Eduati et al.   Nature Communications

Single-Cell RNA-Seq Reveals Transcriptional Heterogeneity in Latent and Reactivated HIV-Infected Cells

(2018) Monica Golumbeanu et al.   Cell Reports

Single-Cell Droplet Microfluidic Screening for Antibodies Specifically Binding to Target Cells

(2018) Nachiket Shembekar et al.   Cell Reports

Droplet microfluidics for the highly controlled synthesis of branched gold nanoparticles

(2018) Sara Abalde-Cela et al.   Scientific Reports

T cell immunoengineering with advanced biomaterials

(2017) Derfogail Delcassian et al.   Integrative Biology

New insights into mammalian signaling pathways using microfluidic pulsatile inputs and mathematical modeling

(2017) M. Sumit et al.   Integrative Biology

Integrated platform for cell culture and dynamic quantification of cell secretion

(2017) Alicia J. Kaestli et al.   LAB ON A CHIP

Characterisation of anticancer peptides at the single-cell level

(2017) L. Armbrecht et al.   LAB ON A CHIP

Deterministic droplet-based co-encapsulation and pairing of microparticles via active sorting and downstream merging

(2017) Meng Ting Chung et al.   LAB ON A CHIP

Controlled droplet discretization and manipulation using membrane displacement traps

(2017) S. Padmanabhan et al.   LAB ON A CHIP

Multivesicular droplets: a cell model system to study compartmentalised biochemical reactions

(2017) N. Nuti et al.   LAB ON A CHIP

Cathepsin S inhibition changes regulatory T-cell activity in regulating bladder cancer and immune cell proliferation and apoptosis

(2017) Xiang Yan et al.   MOLECULAR IMMUNOLOGY

Single-cell genome sequencing at ultra-high-throughput with microfluidic droplet barcoding

(2017) Freeman Lan et al.   NATURE BIOTECHNOLOGY

Single-cell deep phenotyping of IgG-secreting cells for high-resolution immune monitoring

(2017) Klaus Eyer et al.   NATURE BIOTECHNOLOGY

Expansion of patient-derived circulating tumor cells from liquid biopsies using a CTC microfluidic culture device

(2017) Bee Luan Khoo et al.   Nature Protocols

Single-cell analysis of HIV-1 transcriptional activity reveals expression of proviruses in expanded clones during ART

(2017) Ann Wiegand et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A microfluidic chip integrated with droplet generation, pairing, trapping, merging, mixing and releasing

(2017) Xiaoming Chen et al.   RSC Advances

Massively parallel whole genome amplification for single-cell sequencing using droplet microfluidics

(2017) Masahito Hosokawa et al.   Scientific Reports

Human Organ Chip Models Recapitulate Orthotopic Lung Cancer Growth, Therapeutic Responses, and Tumor Dormancy In Vitro

(2017) Bryan A. Hassell et al.   Cell Reports

Dynamic Analysis of Human Natural Killer Cell Response at Single-Cell Resolution in B-Cell Non-Hodgkin Lymphoma

(2017) Saheli Sarkar et al.   Frontiers in Immunology

Single-cell profiling of dynamic cytokine secretion and the phenotype of immune cells

(2017) Xingyue An et al.   PLoS One

Abseq: Ultrahigh-throughput single cell protein profiling with droplet microfluidic barcoding

(2017) Payam Shahi et al.   Scientific Reports

Highly-sensitive capture of circulating tumor cells using micro-ellipse filters

(2017) Hongmei Chen et al.   Scientific Reports

Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells

(2017) Hui Hou et al.   Scientific Reports

Transcriptional landscape of epithelial and immune cell populations revealed through FACS-seq of healthy human skin

(2017) Richard S. Ahn et al.   Scientific Reports

High spatial and temporal resolution cell manipulation techniques in microchannels

(2016) Pedro Novo et al.   ANALYST

A Method for Detecting Circulating Tumor Cells Based on the Measurement of Single-Cell Metabolism in Droplet-Based Microfluidics

(2016) Fabio Del Ben et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enhanced immunotherapy of SM5-1 in hepatocellular carcinoma by conjugating with gold nanoparticles and its in vivo bioluminescence tomographic evaluation

(2016) Xibo Ma et al.   BIOMATERIALS

A novel high-throughput multi-parameter flow cytometry based method for monitoring and rapid characterization of microbiome dynamics in anaerobic systems

(2016) Abhishek S. Dhoble et al.   BIORESOURCE TECHNOLOGY

Controlled assembly of heterotypic cells in a core–shell scaffold: organ in a droplet

(2016) Qiushui Chen et al.   LAB ON A CHIP

Innate cell communication kick-starts pathogen-specific immunity

(2016) Amariliz Rivera et al.   NATURE IMMUNOLOGY

Driving CAR T-cells forward

(2016) Hollie J. Jackson et al.   Nature Reviews Clinical Oncology

Longitudinal multiparameter assay of lymphocyte interactions from onset by microfluidic cell pairing and culture

(2016) Burak Dura et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A microfluidic platform for trapping, releasing and super-resolution imaging of single cells

(2016) Ying Zhou et al.   SENSORS AND ACTUATORS B-CHEMICAL

A microfluidic platform enabling single-cell RNA-seq of multigenerational lineages

(2016) Robert J. Kimmerling et al.   Nature Communications

A microfluidic device for measuring cell migration towards substrate-bound and soluble chemokine gradients

(2016) Jan Schwarz et al.   Scientific Reports

High-Content Quantification of Single-Cell Immune Dynamics

(2016) Michael Junkin et al.   Cell Reports

Microchip Screening Platform for Single Cell Assessment of NK Cell Cytotoxicity

(2016) Karolin Guldevall et al.   Frontiers in Immunology

Constructing 3D heterogeneous hydrogels from electrically manipulated prepolymer droplets and crosslinked microgels

(2016) M.-Y. Chiang et al.   Science Advances

NF-κB signalling and cell fate decisions in response to a short pulse of tumour necrosis factor

(2016) Robin E. C. Lee et al.   Scientific Reports

Single-Cell Genomics Unveils Critical Regulators of Th17 Cell Pathogenicity

(2015) Jellert T. Gaublomme et al.   CELL

Immunoengineering: How Nanotechnology Can Enhance Cancer Immunotherapy

(2015) Michael S. Goldberg   CELL

Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells

(2015) Allon M. Klein et al.   CELL

Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets

(2015) Evan Z. Macosko et al.   CELL

A high-throughput RNAi screen for detection of immune-checkpoint molecules that mediate tumor resistance to cytotoxic T lymphocytes

(2015) N. Khandelwal et al.   EMBO Molecular Medicine

Microfluidic mazes to characterize T-cell exploration patterns following activation in vitro

(2015) Namrata G. Jain et al.   Integrative Biology

Cutting Edge: Redox Signaling Hypersensitivity Distinguishes Human Germinal Center B Cells

(2015) Hannah G. Polikowsky et al.   JOURNAL OF IMMUNOLOGY

Analysis of fast protein phosphorylation kinetics in single cells on a microfluidic chip

(2015) Matthias Blazek et al.   LAB ON A CHIP

Automated co-culture system for spatiotemporal analysis of cell-to-cell communication

(2015) Tino Frank et al.   LAB ON A CHIP

The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation

(2015) David J. Collins et al.   LAB ON A CHIP

Integrating gene synthesis and microfluidic protein analysis for rapid protein engineering

(2015) Matthew C. Blackburn et al.   NUCLEIC ACIDS RESEARCH

Engineering opportunities in cancer immunotherapy

(2015) Laura Jeanbart et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Network representations of immune system complexity

(2015) Naeha Subramanian et al.   Wiley Interdisciplinary Reviews-Systems Biology and Medicine

Profiling lymphocyte interactions at the single-cell level by microfluidic cell pairing

(2015) Burak Dura et al.   Nature Communications

Label-free single-cell protein quantification using a drop-based mix-and-read system

(2015) Alireza Abbaspourrad et al.   Scientific Reports

Digital signaling decouples activation probability and population heterogeneity

(2015) Ryan A Kellogg et al.   eLife

Droplet Manipulations in Two Phase Flow Microfluidics

(2015) Arjen Pit et al.   Micromachines

Hydrogel dual delivered celecoxib and anti-PD-1 synergistically improve antitumor immunity

(2015) Yongkui Li et al.   OncoImmunology

A Microfluidic Platform for High-Throughput Multiplexed Protein Quantitation

(2015) Francesca Volpetti et al.   PLoS One

Microfluidics-Based in Vivo Mimetic Systems for the Study of Cellular Biology

(2014) Donghyuk Kim et al.   ACCOUNTS OF CHEMICAL RESEARCH

Transcription Factor Sensor System for Parallel Quantification of Metabolites On-Chip

(2014) Simon Ketterer et al.   ANALYTICAL CHEMISTRY

Nanowell-Based Immunoassays for Measuring Single-Cell Secretion: Characterization of Transport and Surface Binding

(2014) Alexis J. Torres et al.   ANALYTICAL CHEMISTRY

Screening for Protein Phosphorylation Using Nanoscale Reactions on Microdroplet Arrays

(2014) Simon K. Küster et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A highly parallel microfluidic droplet method enabling single-molecule counting for digital enzyme detection

(2014) Zhichao Guan et al.   Biomicrofluidics

Live cell imaging compatible immobilization ofChlamydomonas reinhardtiiin microfluidic platform for biodiesel research

(2014) Jae Woo Park et al.   BIOTECHNOLOGY AND BIOENGINEERING

Microfluidics-assisted engineering of polymeric microcapsules with high encapsulation efficiency for protein drug delivery

(2014) Jenni Pessi et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Deformability-based microfluidic cell pairing and fusion

(2014) Burak Dura et al.   LAB ON A CHIP

Microfluidic single-cell analysis for systems immunology

(2014) Michael Junkin et al.   LAB ON A CHIP

The present and future role of microfluidics in biomedical research

(2014) Eric K. Sackmann et al.   NATURE

Single-cell RNA-seq reveals dynamic paracrine control of cellular variation

(2014) Alex K. Shalek et al.   NATURE

Single-cell technologies for monitoring immune systems

(2014) Pratip K Chattopadhyay et al.   NATURE IMMUNOLOGY

Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry

(2014) Charlotte Giesen et al.   NATURE METHODS

High-throughput microfluidic single-cell analysis pipeline for studies of signaling dynamics

(2014) Ryan A Kellogg et al.   Nature Protocols

Massively Parallel Single-Cell RNA-Seq for Marker-Free Decomposition of Tissues into Cell Types

(2014) D. A. Jaitin et al.   SCIENCE

Towards efficient cancer immunotherapy: advances in developing artificial antigen-presenting cells

(2014) Loek J. Eggermont et al.   TRENDS IN BIOTECHNOLOGY

Heterogeneity in immune responses: from populations to single cells

(2014) Rahul Satija et al.   TRENDS IN IMMUNOLOGY

One drop at a time: toward droplet microfluidics as a versatile tool for single-cell analysis

(2014) Agata Rakszewska et al.   NPG Asia Materials

An Integrated Microfluidic Chip System for Single-Cell Secretion Profiling of Rare Circulating Tumor Cells

(2014) Yuliang Deng et al.   Scientific Reports

Single-Cell Imaging of Caspase-1 Dynamics Reveals an All-or-None Inflammasome Signaling Response

(2014) Ting Liu et al.   Cell Reports

Tissue-Resident T Cells: Dynamic Players in Skin Immunity

(2014) Scott N. Mueller et al.   Frontiers in Immunology

Bacterial Growth and Adaptation in Microdroplet Chemostats

(2013) Slawomir Jakiela et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Encapsulation of single cells on a microfluidic device integrating droplet generation with fluorescence-activated droplet sorting

(2013) Liang Wu et al.   BIOMEDICAL MICRODEVICES

Networking at the Level of Host Immunity: Immune Cell Interactions during Persistent Viral Infections

(2013) Cherie T. Ng et al.   Cell Host & Microbe

Recent developments in microfluidic large scale integration

(2013) Ismail Emre Araci et al.   CURRENT OPINION IN BIOTECHNOLOGY

Human cell-based artificial antigen-presenting cells for cancer immunotherapy

(2013) Marcus O. Butler et al.   IMMUNOLOGICAL REVIEWS

A high-throughput nanoimmunoassay chip applied to large-scale vaccine adjuvant screening

(2013) Jose L. Garcia-Cordero et al.   Integrative Biology

Flow-switching allows independently programmable, extremely stable, high-throughput diffusion-based gradients

(2013) Tino Frank et al.   LAB ON A CHIP

Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics

(2013) Venkatachalam Chokkalingam et al.   LAB ON A CHIP

Single cell membrane poration by bubble-induced microjets in a microfluidic chip

(2013) Z. G. Li et al.   LAB ON A CHIP

Proximity Ligation Assay for High-content Profiling of Cell Signaling Pathways on a Microfluidic Chip

(2013) Matthias Blazek et al.   MOLECULAR & CELLULAR PROTEOMICS

Combinatorial tetramer staining and mass cytometry analysis facilitate T-cell epitope mapping and characterization

(2013) Evan W Newell et al.   NATURE BIOTECHNOLOGY

The promise of single-cell sequencing

(2013) James Eberwine et al.   NATURE METHODS

Single-cell analysis and sorting using droplet-based microfluidics

(2013) Linas Mazutis et al.   Nature Protocols

Single molecule quantitation and sequencing of rare translocations using microfluidic nested digital PCR

(2013) Joe Shuga et al.   NUCLEIC ACIDS RESEARCH

Th9 cells, new players in adaptive immunity

(2013) Edgar Schmitt et al.   TRENDS IN IMMUNOLOGY

A Mathematical Model of Immune Activation with a Unified Self-Nonself Concept

(2013) Sahamoddin Khailaie et al.   Frontiers in Immunology

Microfluidic single cell analysis: from promise to practice

(2012) Véronique Lecault et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

How the immune system talks to itself: the varied role of synapses

(2012) Jianming Xie et al.   IMMUNOLOGICAL REVIEWS

Single-cell analysis of the dynamics and functional outcomes of interactions between human natural killer cells and target cells

(2012) Yvonne J. Yamanaka et al.   Integrative Biology

High-yield cell ordering and deterministic cell-in-droplet encapsulation using Dean flow in a curved microchannel

(2012) Evelien W. M. Kemna et al.   LAB ON A CHIP

Monitoring the dynamics of primary T cell activation and differentiation using long term live cell imaging in microwell arrays

(2012) Irina Zaretsky et al.   LAB ON A CHIP

Rapid screening of antibiotic toxicity in an automated microdroplet system

(2012) Krzysztof Churski et al.   LAB ON A CHIP

Functional single-cell analysis of T-cell activation by supported lipid bilayer-tethered ligands on arrays of nanowells

(2012) Alexis J. Torres et al.   LAB ON A CHIP

Quantitating Cell–Cell Interaction Functions with Applications to Glioblastoma Multiforme Cancer Cells

(2012) Jun Wang et al.   NANO LETTERS

Massively parallel measurements of molecular interaction kinetics on a microfluidic platform

(2012) M. Geertz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Functional single-cell hybridoma screening using droplet-based microfluidics

(2012) B. E. Debs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering Approaches to Immunotherapy

(2012) M. A. Swartz et al.   Science Translational Medicine

A deep profiler's guide to cytometry

(2012) Sean C. Bendall et al.   TRENDS IN IMMUNOLOGY

The Role of Body-on-a-Chip Devices in Drug and Toxicity Studies

(2011) M.B. Esch et al.   Annual Review of Biomedical Engineering

Microfluidic electrochemical immunoarray for ultrasensitive detection of two cancer biomarker proteins in serum

(2011) Bhaskara V. Chikkaveeraiah et al.   BIOSENSORS & BIOELECTRONICS

A software-programmable microfluidic device for automated biology

(2011) Luis M. Fidalgo et al.   LAB ON A CHIP

Extracting a cellular hierarchy from high-dimensional cytometry data with SPADE

(2011) Peng Qiu et al.   NATURE BIOTECHNOLOGY

A clinical microchip for evaluation of single immune cells reveals high functional heterogeneity in phenotypically similar T cells

(2011) Chao Ma et al.   NATURE MEDICINE

Dendritic cell chemotaxis in 3D under defined chemokine gradients reveals differential response to ligands CCL21 and CCL19

(2011) U. Haessler et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum

(2011) S. C. Bendall et al.   SCIENCE

Microfluidic Measurement of Antibody−Antigen Binding Kinetics from Low-Abundance Samples and Single Cells

(2010) Anupam Singhal et al.   ANALYTICAL CHEMISTRY

Sequential Array Cytometry: Multi-Parameter Imaging with a Single Fluorescent Channel

(2010) Daniel R. Gossett et al.   ANNALS OF BIOMEDICAL ENGINEERING

Variability in G-Protein-Coupled Signaling Studied with Microfluidic Devices

(2010) Xiaoyan Robert Bao et al.   BIOPHYSICAL JOURNAL

Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine

(2010) Tania Konry et al.   BIOSENSORS & BIOELECTRONICS

Artificial Antigen-Presenting Cells for Use in Adoptive Immunotherapy

(2010) Cameron J. Turtle et al.   CANCER JOURNAL

Optimization of microfluidic single cell trapping for long-term on-chip culture

(2010) Stefan Kobel et al.   LAB ON A CHIP

Single-cell NF-κB dynamics reveal digital activation and analogue information processing

(2010) Savaş Tay et al.   NATURE

Whole-genome molecular haplotyping of single cells

(2010) H Christina Fan et al.   NATURE BIOTECHNOLOGY

A Systems Model for Immune Cell Interactions Unravels the Mechanism of Inflammation in Human Skin

(2010) Najl V. Valeyev et al.   PLoS Computational Biology

Control of Regulatory T Cell Lineage Commitment and Maintenance

(2009) Steven Z. Josefowicz et al.   IMMUNITY

Th22 cells represent a distinct human T cell subset involved in epidermal immunity and remodeling

(2009) Stefanie Eyerich et al.   JOURNAL OF CLINICAL INVESTIGATION

Correlating short-term Ca2+ responses with long-term protein expression after activation of single T cells

(2009) Michael Kirschbaum et al.   LAB ON A CHIP

Surface acoustic wave (SAW) directed droplet flow in microfluidics for PDMS devices

(2009) Thomas Franke et al.   LAB ON A CHIP

Microfluidic single cell arrays to interrogate signalling dynamics of individual, patient-derived hematopoietic stem cells

(2009) Shannon L. Faley et al.   LAB ON A CHIP

Microfluidic control of cell pairing and fusion

(2009) Alison M Skelley et al.   NATURE METHODS

Dynamic analysis of MAPK signaling using a high-throughput microfluidic single-cell imaging platform

(2009) R. J. Taylor et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A microfluidic device for multiplexed protein detection in nano-liter volumes

(2008) Alan H. Diercks et al.   ANALYTICAL BIOCHEMISTRY

Cell Culture Models in Microfluidic Systems

(2008) Ivar Meyvantsson et al.   Annual Review of Analytical Chemistry

Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms

(2008) Jenifer Clausell-Tormos et al.   CHEMISTRY & BIOLOGY

Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

(2008) Ansgar Huebner et al.   LAB ON A CHIP

Integrated microfluidic bioprocessor for single-cell gene expression analysis

(2008) N. M. Toriello et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A microfluidic processor for gene expression profiling of single human embryonic stem cells

(2007) Jiang F. Zhong et al.   LAB ON A CHIP