Single-cell technologies for monitoring immune systems

High-Throughput Secretomic Analysis of Single Cells to Assess Functional Cellular Heterogeneity

(2013) Yao Lu et al.   ANALYTICAL CHEMISTRY

Mixture models for single-cell assays with applications to vaccine studies

(2013) G. Finak et al.   BIOSTATISTICS

Classification of human natural killer cells based on migration behavior and cytotoxic response

(2013) B. Vanherberghen et al.   BLOOD

Immunoliposome encapsulation increases cytotoxic activity and selectivity of curcumin and resveratrol against HER2 overexpressing human breast cancer cells

(2013) Angela Catania et al.   BREAST CANCER RESEARCH AND TREATMENT

Advantages of full spectrum flow cytometry

(2013) Claire K. Sanders et al.   JOURNAL OF BIOMEDICAL OPTICS

Highly multiplexed quantitation of gene expression on single cells

(2013) Maria H. Dominguez et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Detection and isolation of auto-reactive human antibodies from primary B cells

(2013) Victor G. Sendra et al.   METHODS

Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells

(2013) Alex K. Shalek et al.   NATURE

viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemia

(2013) El-ad David Amir et al.   NATURE BIOTECHNOLOGY

Critical assessment of automated flow cytometry data analysis techniques

(2013) Nima Aghaeepour et al.   NATURE METHODS

Surface Area Loss and Increased Sphericity Account for the Splenic Entrapment of Subpopulations of Plasmodium falciparum Ring-Infected Erythrocytes

(2013) Innocent Safeukui et al.   PLoS One

High throughput HLA genotyping using 454 sequencing and the Fluidigm Access Array™ system for simplified amplicon library preparation

(2013) P. V. Moonsamy et al.   TISSUE ANTIGENS

Early immunologic correlates of HIV protection can be identified from computational analysis of complex multivariate T-cell flow cytometry assays*

(2012) Nima Aghaeepour et al.   BIOINFORMATICS

Data exploration, quality control and testing in single-cell qPCR-based gene expression experiments

(2012) Andrew McDavid et al.   BIOINFORMATICS

RchyOptimyx: Cellular hierarchy optimization for flow cytometry

(2012) Nima Aghaeepour et al.   CYTOMETRY PART A

Brilliant violet fluorophores: A new class of ultrabright fluorescent compounds for immunofluorescence experiments

(2012) Pratip K. Chattopadhyay et al.   CYTOMETRY PART A

Cytometry by Time-of-Flight Shows Combinatorial Cytokine Expression and Virus-Specific Cell Niches within a Continuum of CD8+ T Cell Phenotypes

(2012) Evan W. Newell et al.   IMMUNITY

Transcription Factor Foxo1 Represses T-bet-Mediated Effector Functions and Promotes Memory CD8+ T Cell Differentiation

(2012) Rajesh R. Rao et al.   IMMUNITY

Single-cell analysis of the dynamics and functional outcomes of interactions between human natural killer cells and target cells

(2012) Yvonne J. Yamanaka et al.   Integrative Biology

Single-cell level co-culture platform for intercellular communication

(2012) Soongweon Hong et al.   Integrative Biology

Monitoring the dynamics of primary T cell activation and differentiation using long term live cell imaging in microwell arrays

(2012) Irina Zaretsky et al.   LAB ON A CHIP

Functional single-cell analysis of T-cell activation by supported lipid bilayer-tethered ligands on arrays of nanowells

(2012) Alexis J. Torres et al.   LAB ON A CHIP

Quantitating Cell–Cell Interaction Functions with Applications to Glioblastoma Multiforme Cancer Cells

(2012) Jun Wang et al.   NANO LETTERS

Multiplexed mass cytometry profiling of cellular states perturbed by small-molecule regulators

(2012) Bernd Bodenmiller et al.   NATURE BIOTECHNOLOGY

Rapid, efficient functional characterization and recovery of HIV-specific human CD8+ T cells using microengraving

(2012) N. Varadarajan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A deep profiler's guide to cytometry

(2012) Sean C. Bendall et al.   TRENDS IN IMMUNOLOGY

A silicon-glass microwell platform for high-resolution imaging and high-content screening with single cell resolution

(2011) Thomas Wilhelm Frisk et al.   BIOMEDICAL MICRODEVICES

Microfluidics for single cell analysis

(2011) Huabing Yin et al.   CURRENT OPINION IN BIOTECHNOLOGY

Quantifying nuclear p65 as a parameter for NF-κB activation: Correlation between ImageStream cytometry, microscopy, and Western blot

(2011) Orla Maguire et al.   CYTOMETRY PART A

L-plastin phosphorylation: A novel target for the immunosuppressive drug dexamethasone in primary human T cells

(2011) Guido H. Wabnitz et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

Analysis of transient migration behavior of natural killer cells imaged in situ and in vitro

(2011) Mohammad Ali Khorshidi et al.   Integrative Biology

A high-throughput single-cell analysis of human CD8+ T cell functions reveals discordance for cytokine secretion and cytolysis

(2011) Navin Varadarajan et al.   JOURNAL OF CLINICAL INVESTIGATION

Extracting a cellular hierarchy from high-dimensional cytometry data with SPADE

(2011) Peng Qiu et al.   NATURE BIOTECHNOLOGY

A clinical microchip for evaluation of single immune cells reveals high functional heterogeneity in phenotypically similar T cells

(2011) Chao Ma et al.   NATURE MEDICINE

Deterministic and Stochastic Allele Specific Gene Expression in Single Mouse Blastomeres

(2011) Fuchou Tang et al.   PLoS One

Single-cell proteomic chip for profiling intracellular signaling pathways in single tumor cells

(2011) Q. Shi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Polyfunctional responses by human T cells result from sequential release of cytokines

(2011) Q. Han et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum

(2011) S. C. Bendall et al.   SCIENCE

Integrated process design for single-cell analytical technologies

(2010) J. Christopher Love   AICHE JOURNAL

Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine

(2010) Tania Konry et al.   BIOSENSORS & BIOELECTRONICS

Good cell, bad cell: Flow cytometry reveals T-cell subsets important in HIV disease

(2010) Pratip K. Chattopadhyay et al.   CYTOMETRY PART A

Targeting of Cancer Cells Using Click-Functionalized Polymer Capsules

(2010) Marloes M. J. Kamphuis et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Multidimensional analysis of the frequencies and rates of cytokine secretion from single cells by quantitative microengraving

(2010) Qing Han et al.   LAB ON A CHIP

Massively parallel detection of gene expression in single cells using subnanolitre wells

(2010) Yuan Gong et al.   LAB ON A CHIP

Overview of single-cell analyses: microdevices and applications

(2010) Sara Lindström et al.   LAB ON A CHIP

A multiplex assay for the simultaneous detection of antibodies against 15 Plasmodium falciparum and Anopheles gambiae saliva antigens

(2010) Elena Ambrosino et al.   MALARIA JOURNAL

Imaging Immune Surveillance of Individual Natural Killer Cells Confined in Microwell Arrays

(2010) Karolin Guldevall et al.   PLoS One

Detecting Cytokine Release from Single T-cells

(2009) He Zhu et al.   ANALYTICAL CHEMISTRY

A cell chip for sequential imaging of individual non-adherent live cells reveals transients and oscillations

(2009) Yael S. Schiffenbauer et al.   LAB ON A CHIP

A rapid and efficient single-cell manipulation method for screening antigen-specific antibody–secreting cells from human peripheral blood

(2009) Aishun Jin et al.   NATURE MEDICINE

Simultaneous detection of many T-cell specificities using combinatorial tetramer staining

(2009) Evan W Newell et al.   NATURE METHODS

Droplet microfluidic technology for single-cell high-throughput screening

(2009) E. Brouzes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Concurrent detection of secreted products from human lymphocytes by microengraving: Cytokines and antigen-reactive antibodies

(2008) Elizabeth M. Bradshaw et al.   CLINICAL IMMUNOLOGY

Phenotype and function of human T lymphocyte subsets: Consensus and issues

(2008) Victor Appay et al.   CYTOMETRY PART A

Yellow fever vaccine induces integrated multilineage and polyfunctional immune responses

(2008) Denis Gaucher et al.   JOURNAL OF EXPERIMENTAL MEDICINE