Microfluidic single-cell analysis for systems immunology

High-Throughput Secretomic Analysis of Single Cells to Assess Functional Cellular Heterogeneity

(2013) Yao Lu et al.   ANALYTICAL CHEMISTRY

Classification of human natural killer cells based on migration behavior and cytotoxic response

(2013) B. Vanherberghen et al.   BLOOD

Microfluidic cell culture

(2013) Matthias Mehling et al.   CURRENT OPINION IN BIOTECHNOLOGY

Complex chemoattractive and chemorepellent Kit signals revealed by direct imaging of murine mast cells in microfluidic gradient chambers

(2013) Amir Shamloo et al.   Integrative Biology

Live cell imaging in a micro-array of acoustic traps facilitates quantification of natural killer cell heterogeneity

(2013) Athanasia E. Christakou et al.   Integrative Biology

Nuclear Envelope Composition Determines the Ability of Neutrophil-type Cells to Passage through Micron-scale Constrictions

(2013) Amy C. Rowat et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Flow-switching allows independently programmable, extremely stable, high-throughput diffusion-based gradients

(2013) Tino Frank et al.   LAB ON A CHIP

Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics

(2013) Venkatachalam Chokkalingam et al.   LAB ON A CHIP

Microfluidic platforms for RNA interference screening of virus–host interactions

(2013) Benjamin R. Schudel et al.   LAB ON A CHIP

Quantitative assessment of single-cell RNA-sequencing methods

(2013) Angela R Wu et al.   NATURE METHODS

A chemostat array enables the spatio-temporal analysis of the yeast proteome

(2013) N. Denervaud et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genomic responses in mouse models poorly mimic human inflammatory diseases

(2013) Junhee Seok et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Accelerating Next-Generation Vaccine Development for Global Disease Prevention

(2013) W. C. Koff et al.   SCIENCE

Single-Cell Metabolomics: Analytical and Biological Perspectives

(2013) R. Zenobi   SCIENCE

Microdevice integrating innate and adaptive immune responses associated with antigen presentation by dendritic cells

(2013) Bhaskar Mitra et al.   RSC Advances

Cell Assays: Surface-Micromachined Microfiltration Membranes for Efficient Isolation and Functional Immunophenotyping of Subpopulations of Immune Cells (Adv. Healthcare Mater. 7/2013)

(2013) Weiqiang Chen et al.   Advanced Healthcare Materials

Low-Volume Toolbox for the Discovery of Immunosuppressive Fungal Secondary Metabolites

(2013) Erwin Berthier et al.   PLoS Pathogens

Inflammatory mimetic microfluidic chip by immobilization of cell adhesion molecules for T cell adhesion

(2012) Sung Kyu Kim et al.   ANALYST

Neutrophil Chemotaxis within a Competing Gradient of Chemoattractants

(2012) Donghyuk Kim et al.   ANALYTICAL CHEMISTRY

Correlative Analyses of Nitric Oxide Generation Rates and Nitric Oxide Synthase Levels in Individual Cells Using a Modular Cell-Retaining Device

(2012) Yana Shafran et al.   ANALYTICAL CHEMISTRY

Observing Single Cell NF-κB Dynamics under Stimulant Concentration Gradient

(2012) Yousef Awwad et al.   ANALYTICAL CHEMISTRY

Cellular Barcodes for Efficiently Profiling Single-Cell Secretory Responses by Microengraving

(2012) Yvonne J. Yamanaka et al.   ANALYTICAL CHEMISTRY

Simultaneous detection of cell-secreted TNF-α and IFN-γ using micropatterned aptamer-modified electrodes

(2012) Ying Liu et al.   BIOMATERIALS

Single-cell analysis of the dynamics and functional outcomes of interactions between human natural killer cells and target cells

(2012) Yvonne J. Yamanaka et al.   Integrative Biology

Toll/Interleukin-1 Receptor Domain-Containing Adapter Inducing Interferon-  Mediates Microglial Phagocytosis of Degenerating Axons

(2012) S. Hosmane et al.   JOURNAL OF NEUROSCIENCE

Microfluidically-unified cell culture, sample preparation, imaging and flow cytometry for measurement of cell signaling pathways with single cell resolution

(2012) Meiye Wu et al.   LAB ON A CHIP

Monitoring the dynamics of primary T cell activation and differentiation using long term live cell imaging in microwell arrays

(2012) Irina Zaretsky et al.   LAB ON A CHIP

High throughput automated chromatin immunoprecipitation as a platform for drug screening and antibody validation

(2012) Angela R. Wu et al.   LAB ON A CHIP

A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils

(2012) Sewoon Han et al.   LAB ON A CHIP

An integrated microfluidic platform for in situ cellular cytokine secretion immunophenotyping

(2012) Nien-Tsu Huang et al.   LAB ON A CHIP

Functional single-cell analysis of T-cell activation by supported lipid bilayer-tethered ligands on arrays of nanowells

(2012) Alexis J. Torres et al.   LAB ON A CHIP

Cross talk between cancer and immune cells: exploring complex dynamics in a microfluidic environment

(2012) Luca Businaro et al.   LAB ON A CHIP

‘Slings’ enable neutrophil rolling at high shear

(2012) Prithu Sundd et al.   NATURE

A Novel Rabbit Immunospot Array Assay on a Chip Allows for the Rapid Generation of Rabbit Monoclonal Antibodies with High Affinity

(2012) Tatsuhiko Ozawa et al.   PLoS One

Microfluidic chambers for monitoring leukocyte trafficking and humanized nano-proresolving medicines interactions

(2012) C. N. Jones et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Migration of cells in a social context

(2012) S. Vedel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Hydrodynamic stretching of single cells for large population mechanical phenotyping

(2012) D. R. Gossett et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rapid, efficient functional characterization and recovery of HIV-specific human CD8+ T cells using microengraving

(2012) N. Varadarajan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Functional single-cell hybridoma screening using droplet-based microfluidics

(2012) B. E. Debs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Activation-Induced B Cell Fates Are Selected by Intracellular Stochastic Competition

(2012) K. R. Duffy et al.   SCIENCE

Monitoring the status of T-cell activation in a microfluidic system

(2011) Joo Young Park et al.   ANALYST

Micropatterned Aptasensors for Continuous Monitoring of Cytokine Release from Human Leukocytes

(2011) Ying Liu et al.   ANALYTICAL CHEMISTRY

A silicon-glass microwell platform for high-resolution imaging and high-content screening with single cell resolution

(2011) Thomas Wilhelm Frisk et al.   BIOMEDICAL MICRODEVICES

Electrochemical biosensors for on-chip detection of oxidative stress from immune cells

(2011) Jun Yan et al.   Biomicrofluidics

Measuring Traction Forces of Motile Dendritic Cells on Micropost Arrays

(2011) Brendon G. Ricart et al.   BIOPHYSICAL JOURNAL

Protein Signaling Networks from Single Cell Fluctuations and Information Theory Profiling

(2011) Young Shik Shin et al.   BIOPHYSICAL JOURNAL

Subsets of human CD4+ regulatory T cells express the peripheral homing receptor CXCR3

(2011) André Hoerning et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

Cytotoxic T lymphocyte perforin and Fas ligand working in concert even when Fas ligand lytic action is still not detectable

(2011) David Hassin et al.   IMMUNOLOGY

Analysis of transient migration behavior of natural killer cells imaged in situ and in vitro

(2011) Mohammad Ali Khorshidi et al.   Integrative Biology

Rapid selection of single cells with high antibody production rates by microwell array

(2011) Sunyoung Park et al.   JOURNAL OF BIOTECHNOLOGY

A high-throughput single-cell analysis of human CD8+ T cell functions reveals discordance for cytokine secretion and cytolysis

(2011) Navin Varadarajan et al.   JOURNAL OF CLINICAL INVESTIGATION

Image-based analysis of primary human neutrophil chemotaxis in an automated direct-viewing assay

(2011) Ivar Meyvantsson et al.   JOURNAL OF IMMUNOLOGICAL METHODS

In silico feedback for in vivo regulation of a gene expression circuit

(2011) Andreas Milias-Argeitis et al.   NATURE BIOTECHNOLOGY

A clinical microchip for evaluation of single immune cells reveals high functional heterogeneity in phenotypically similar T cells

(2011) Chao Ma et al.   NATURE MEDICINE

Combinatorial Guidance by CCR7 Ligands for T Lymphocytes Migration in Co-Existing Chemokine Fields

(2011) Saravanan Nandagopal et al.   PLoS One

Dendritic cell chemotaxis in 3D under defined chemokine gradients reveals differential response to ligands CCL21 and CCL19

(2011) U. Haessler et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Polyfunctional responses by human T cells result from sequential release of cytokines

(2011) Q. Han et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Systems Biology Approach to Infectious Disease Research: Innovating the Pathogen-Host Research Paradigm

(2011) A. Aderem et al.   mBio

Microfluidic Measurement of Antibody−Antigen Binding Kinetics from Low-Abundance Samples and Single Cells

(2010) Anupam Singhal et al.   ANALYTICAL CHEMISTRY

Polymer live-cell array for real-time kinetic imaging of immune cells

(2010) Naomi Zurgil et al.   BIOMATERIALS

Variability in G-Protein-Coupled Signaling Studied with Microfluidic Devices

(2010) Xiaoyan Robert Bao et al.   BIOPHYSICAL JOURNAL

Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine

(2010) Tania Konry et al.   BIOSENSORS & BIOELECTRONICS

Directional decisions during neutrophil chemotaxis inside bifurcating channels

(2010) Vijayakrishnan Ambravaneswaran et al.   Integrative Biology

Biological applications of microfluidic gradient devices

(2010) Sudong Kim et al.   Integrative Biology

Dendritic Cells Distinguish Individual Chemokine Signals through CCR7 and CXCR4

(2010) B. G. Ricart et al.   JOURNAL OF IMMUNOLOGY

Massively parallel detection of gene expression in single cells using subnanolitre wells

(2010) Yuan Gong et al.   LAB ON A CHIP

Functional roles for noise in genetic circuits

(2010) Avigdor Eldar et al.   NATURE

Single-cell NF-κB dynamics reveal digital activation and analogue information processing

(2010) Savaş Tay et al.   NATURE

Burn Injury Reduces Neutrophil Directional Migration Speed in Microfluidic Devices

(2010) Kathryn L. Butler et al.   PLoS One

Imaging Immune Surveillance of Individual Natural Killer Cells Confined in Microwell Arrays

(2010) Karolin Guldevall et al.   PLoS One

Unveiling the role of network and systems biology in drug discovery

(2010) Albert Pujol et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Fully Integrated Microfluidic Platform Enabling Automated Phosphoprofiling of Macrophage Response

(2009) Nimisha Srivastava et al.   ANALYTICAL CHEMISTRY

Detecting Cytokine Release from Single T-cells

(2009) He Zhu et al.   ANALYTICAL CHEMISTRY

On-Chip Activation and Subsequent Detection of Individual Antigen-Specific T Cells

(2009) Qing Song et al.   ANALYTICAL CHEMISTRY

AlphaLISA Immunoassay Platform— The “No-Wash” High-Throughput Alternative to ELISA

(2009) Martina Bielefeld-Sevigny   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

An agarose-based microfluidic platform with a gradient buffer for 3D chemotaxis studies

(2009) Ulrike Haessler et al.   BIOMEDICAL MICRODEVICES

Identification of antigen-specific B cells by concurrent monitoring of intracellular Ca2+mobilization and antigen binding with microwell array chip system equipped with a CCD imager

(2009) Koshi Kinoshita et al.   CYTOMETRY PART A

Fluid Flow Regulates Stromal Cell Organization and CCL21 Expression in a Tissue-Engineered Lymph Node Microenvironment

(2009) A. A. Tomei et al.   JOURNAL OF IMMUNOLOGY

Correlating short-term Ca2+ responses with long-term protein expression after activation of single T cells

(2009) Michael Kirschbaum et al.   LAB ON A CHIP

A cell chip for sequential imaging of individual non-adherent live cells reveals transients and oscillations

(2009) Yael S. Schiffenbauer et al.   LAB ON A CHIP

Microfluidic single cell arrays to interrogate signalling dynamics of individual, patient-derived hematopoietic stem cells

(2009) Shannon L. Faley et al.   LAB ON A CHIP

A new method for studying gradient-induced neutrophil desensitization based on an open microfluidic chamber

(2009) Thomas M. Keenan et al.   LAB ON A CHIP

Population context determines cell-to-cell variability in endocytosis and virus infection

(2009) Berend Snijder et al.   NATURE

A rapid and efficient single-cell manipulation method for screening antigen-specific antibody–secreting cells from human peripheral blood

(2009) Aishun Jin et al.   NATURE MEDICINE

Screening individual hybridomas by microengraving to discover monoclonal antibodies

(2009) Adebola O Ogunniyi et al.   Nature Protocols

Dynamic analysis of MAPK signaling using a high-throughput microfluidic single-cell imaging platform

(2009) R. J. Taylor et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Droplet microfluidic technology for single-cell high-throughput screening

(2009) E. Brouzes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A method for prolonged imaging of motile lymphocytes

(2008) Daniel Day et al.   IMMUNOLOGY AND CELL BIOLOGY

High Content Cell Screening in a Microfluidic Device

(2008) Raymond Cheong et al.   MOLECULAR & CELLULAR PROTEOMICS

Systems biology of persistent infection: tuberculosis as a case study

(2008) Douglas Young et al.   NATURE REVIEWS MICROBIOLOGY

Dynamic Proteomics of Individual Cancer Cells in Response to a Drug

(2008) A. A. Cohen et al.   SCIENCE