Co-evolution of affinity and stability of grafted amyloid-motif domain antibodies

Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process

(2015) Gillian Houlihan et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Directed Evolution of Human Heavy Chain Variable Domain (VH) Using In Vivo Protein Fitness Filter

(2014) Dong-Sik Kim et al.   PLoS One

Enhanced stabilization of a stable single domain antibody for SEB toxin by random mutagenesis and stringent selection

(2014) K. B. Turner et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Optimal charged mutations in the complementarity-determining regions that prevent domain antibody aggregation are dependent on the antibody scaffold

(2014) J. M. Perchiacca et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Mutational Analysis of 48G7 Reveals that Somatic Hypermutation Affects Both Antibody Stability and Binding Affinity

(2013) Sophie B. Sun et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

CDR-restricted engineering of native human scFvs creates highly stable and soluble bifunctional antibodies for subcutaneous delivery

(2013) Brian J Fennell et al.   mAbs

Rapid optimization and prototyping for therapeutic antibody-like molecules

(2013) Lihui Xu et al.   mAbs

Antibody light chain variable domains and their biophysically improved versions for human immunotherapy

(2013) Dae Young Kim et al.   mAbs

Somatic hypermutation maintains antibody thermodynamic stability during affinity maturation

(2013) Feng Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Stability assessment on a library scale: a rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc

(2013) Christoph Hasenhindl et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Loop-Sequence Features and Stability Determinants in Antibody Variable Domains by High-Throughput Experiments

(2013) Hung-Ju Chang et al.   STRUCTURE

Directed evolution of proteins for increased stability and expression using yeast display

(2012) Michael W. Traxlmayr et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Directed evolution of stabilized IgG1-Fc scaffolds by application of strong heat shock to libraries displayed on yeast

(2012) Michael W. Traxlmayr et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

General strategy for the generation of human antibody variable domains with increased aggregation resistance

(2012) K. Dudgeon et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Aggregation-resistant domain antibodies engineered with charged mutations near the edges of the complementarity-determining regions

(2012) J. M. Perchiacca et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Beyond natural antibodies: the power of in vitro display technologies

(2011) Andrew R M Bradbury et al.   NATURE BIOTECHNOLOGY

Structure-based design of conformation- and sequence-specific antibodies against amyloid  

(2011) J. M. Perchiacca et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Stability and CDR Composition Biases Enrich Binder Functionality Landscapes

(2010) Benjamin J. Hackel et al.   JOURNAL OF MOLECULAR BIOLOGY

Development trends for human monoclonal antibody therapeutics

(2010) Aaron L. Nelson et al.   NATURE REVIEWS DRUG DISCOVERY

Identification and engineering of human variable regions that allow expression of stable single-chain T cell receptors

(2010) D. H. Aggen et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Stability engineering of scFvs for the development of bispecific and multivalent antibodies

(2010) Brian R. Miller et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Grafting of protein L-binding activity onto recombinant antibody fragments

(2009) Julien Muzard et al.   ANALYTICAL BIOCHEMISTRY

Engineering of stable bispecific antibodies targeting IL-17A and IL-23

(2009) Robert Mabry et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Thermodynamically Stable Aggregation-Resistant Antibody Domains through Directed Evolution

(2008) Kristoffer Famm et al.   JOURNAL OF MOLECULAR BIOLOGY

Aggregation-resistant VHs selected by in vitro evolution tend to have disulfide-bonded loops and acidic isoelectric points

(2008) M. Arbabi-Ghahroudi et al.   PROTEIN ENGINEERING DESIGN & SELECTION