Optimal charged mutations in the complementarity-determining regions that prevent domain antibody aggregation are dependent on the antibody scaffold

Alternative Computational Protocols for Supercharging Protein Surfaces for Reversible Unfolding and Retention of Stability

(2013) Bryan S. Der et al.   PLoS One

Toward a Molecular Understanding of Protein Solubility: Increased Negative Surface Charge Correlates with Increased Solubility

(2012) Ryan M. Kramer et al.   BIOPHYSICAL JOURNAL

Structure-Based Design of Supercharged, Highly Thermoresistant Antibodies

(2012) Aleksandr E. Miklos et al.   CHEMISTRY & BIOLOGY

The Influence of Charge Distribution on Self-Association and Viscosity Behavior of Monoclonal Antibody Solutions

(2012) Sandeep Yadav et al.   MOLECULAR PHARMACEUTICS

General strategy for the generation of human antibody variable domains with increased aggregation resistance

(2012) K. Dudgeon et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Aggregation-resistant domain antibodies engineered with charged mutations near the edges of the complementarity-determining regions

(2012) J. M. Perchiacca et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Engineering Aggregation-Resistant Antibodies

(2012) Joseph M. Perchiacca et al.   Annual Review of Chemical and Biomolecular Engineering

Establishing a Link Between Amino Acid Sequences and Self-Associating and Viscoelastic Behavior of Two Closely Related Monoclonal Antibodies

(2011) Sandeep Yadav et al.   PHARMACEUTICAL RESEARCH

Structure-based design of conformation- and sequence-specific antibodies against amyloid  

(2011) J. M. Perchiacca et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Determination of the dipole moments of RNAse SA wild type and a basic mutant

(2011) Ravi Chari et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Mutational analysis of domain antibodies reveals aggregation hotspots within and near the complementarity determining regions

(2011) Joseph M. Perchiacca et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Calibrative approaches to protein solubility modeling of a mutant series using physicochemical descriptors

(2010) William F. Long et al.   JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN

Potential aggregation prone regions in biotherapeutics

(2010) Xiaoling Wang et al.   mAbs

Exploring the sequence determinants of amyloid structure using position-specific scoring matrices

(2010) Sebastian Maurer-Stroh et al.   NATURE METHODS

Potential Aggregation-Prone Regions in Complementarity-Determining Regions of Antibodies and Their Contribution Towards Antigen Recognition: A Computational Analysis

(2010) Xiaoling Wang et al.   PHARMACEUTICAL RESEARCH

Physico-chemical determinants of soluble intrabody expression in mammalian cell cytoplasm

(2010) Erik Kvam et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Structure-based engineering of a monoclonal antibody for improved solubility

(2010) S.-J. Wu et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Formulation and manufacturability of biologics

(2009) Steven J Shire   CURRENT OPINION IN BIOTECHNOLOGY

Bimodal protein solubility distribution revealed by an aggregation analysis of the entire ensemble of Escherichia coli proteins

(2009) T. Niwa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Design of therapeutic proteins with enhanced stability

(2009) N. Chennamsetty et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Aggregation-resistant VHs selected by in vitro evolution tend to have disulfide-bonded loops and acidic isoelectric points

(2008) M. Arbabi-Ghahroudi et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Sequence determinants of protein aggregation in human VH domains

(2008) K. Dudgeon et al.   PROTEIN ENGINEERING DESIGN & SELECTION