Can too many copies spoil the broth?

Mutant strains of Pichia pastoris with enhanced secretion of recombinant proteins

(2013) Sasha Larsen et al.   BIOTECHNOLOGY LETTERS

The effect of gene copy number and co-expression of chaperone on production of albumin fusion proteins in Pichia pastoris

(2012) Qi Shen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

(2012) Laura Näätsaari et al.   PLoS One

Systematic Single-Cell Analysis of Pichia pastoris Reveals Secretory Capacity Limits Productivity

(2012) Kerry Routenberg Love et al.   PLoS One

A color-based stable multi-copy integrant selection system forPichia pastorisusing the attenuatedADE1andADE2genes as auxotrophic markers

(2012) Min Du et al.   Bioengineered

Activation of the unfolded protein response inPichia pastorisrequires splicing of aHAC1mRNA intron and retention of the C-terminal tail of Hac1p

(2011) Graham Whyteside et al.   FEBS LETTERS

Putting the brakes on the unfolded protein response: Figure 1.

(2011) Frank Sicheri et al.   JOURNAL OF CELL BIOLOGY

Native-State Stability Determines the Extent of Degradation Relative to Secretion of Protein Variants from Pichia pastoris

(2011) Graham Whyteside et al.   PLoS One

Road to Ruin: Targeting Proteins for Degradation in the Endoplasmic Reticulum

(2011) M. H. Smith et al.   SCIENCE

Bioactivity and pharmacokinetics of two human serum albumin–thymosin α1-fusion proteins, rHSA-Tα1 and rHSA-L-Tα1, expressed in recombinant Pichia pastoris

(2010) Jian-Hua Chen et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins

(2010) Mouna Guerfal et al.   Microbial Cell Factories

Misfolded proteins impose a dosage-dependent fitness cost and trigger a cytosolic unfolded protein response in yeast

(2010) K. A. Geiler-Samerotte et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Improvement of arachidonic acid and eicosapentaenoic acid production by increasing the copy number of the genes encoding fatty acid desaturase and elongase into Pichia pastoris

(2009) Yun-Tao Li et al.   BIOTECHNOLOGY LETTERS

A systematical investigation on the genetic stability of multi-copy Pichia pastoris strains

(2009) Taicheng Zhu et al.   BIOTECHNOLOGY LETTERS

Directed gene copy number amplification inPichia pastorisby vector integration into the ribosomal DNA locus

(2009) Hans Marx et al.   FEMS YEAST RESEARCH

Efficient generation of multi-copy strains for optimizing secretory expression of porcine insulin precursor in yeastPichia pastoris

(2009) T. Zhu et al.   JOURNAL OF APPLIED MICROBIOLOGY

Membrane expansion alleviates endoplasmic reticulum stress independently of the unfolded protein response

(2009) Sebastian Schuck et al.   JOURNAL OF CELL BIOLOGY

Posttransformational vector amplification in the yeastPichia pastoris

(2008) Anthony Jay Sunga et al.   FEMS YEAST RESEARCH

Direct selection ofPichia pastoris expression strains using new G418 resistance vectors

(2008) Joan Lin-Cereghino et al.   YEAST