Developing Bacillus spp. as a cell factory for production of microbial enzymes and industrially important biochemicals in the context of systems and synthetic biology

Bacillus subtilis: from soil bacterium to super-secreting cell factory

(2013) Jan Maarten van Dijl et al.   Microbial Cell Factories

Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs

(2013) Dokyun Na et al.   NATURE BIOTECHNOLOGY

Generation of an Artificial Double Promoter for Protein Expression in Bacillus subtilis through a Promoter Trap System

(2013) Mingming Yang et al.   PLoS One

Construction of an artificial secYEG operon allowing high level secretion of α-amylase

(2013) Kelly C.L. Mulder et al.   PROTEIN EXPRESSION AND PURIFICATION

Recombinant Bacillus subtilis That Grows on Untreated Plant Biomass

(2012) Timothy D. Anderson et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Effects of Different Amino Acids in Culture Media on Surfactin Variants Produced by Bacillus subtilis TD7

(2012) Jin-Feng Liu et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Enhanced production of 2,3-butanediol by engineered Bacillus subtilis

(2012) Ranjita Biswas et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Genome-scale metabolic model in guiding metabolic engineering of microbial improvement

(2012) Chuan Xu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

High-level exogenous glutamic acid-independent production of poly-(γ-glutamic acid) with organic acid addition in a new isolated Bacillus subtilis C10

(2012) Huili Zhang et al.   BIORESOURCE TECHNOLOGY

Economical production of poly(γ-glutamic acid) using untreated cane molasses and monosodium glutamate waste liquor by Bacillus subtilis NX-2

(2012) Dan Zhang et al.   BIORESOURCE TECHNOLOGY

Improvement of surfactin production in Bacillus subtilis using synthetic wastewater by overexpression of specific extracellular signaling peptides, comX and phrC

(2012) Ju Jung et al.   BIOTECHNOLOGY AND BIOENGINEERING

Novel Twin-Arginine Translocation Pathway-Dependent Phenotypes of Bacillus subtilis Unveiled by Quantitative Proteomics

(2012) Vivianne J. Goosens et al.   JOURNAL OF PROTEOME RESEARCH

High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by Bacillus subtilis

(2012) Kenji Manabe et al.   Microbial Cell Factories

Bacillus subtilis genome editing using ssDNA with short homology regions

(2012) Yang Wang et al.   NUCLEIC ACIDS RESEARCH

Building the repertoire of dispensable chromosome regions in Bacillus subtilis entails major refinement of cognate large-scale metabolic model

(2012) Kosei Tanaka et al.   NUCLEIC ACIDS RESEARCH

Global Network Reorganization During Dynamic Adaptations of Bacillus subtilis Metabolism

(2012) J. M. Buescher et al.   SCIENCE

Condition-Dependent Transcriptome Reveals High-Level Regulatory Architecture in Bacillus subtilis

(2012) P. Nicolas et al.   SCIENCE

Simple Cloning via Direct Transformation of PCR Product (DNA Multimer) to Escherichia coli and Bacillus subtilis

(2011) Chun You et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Self-Inducible Bacillus subtilis Expression System for Reliable and Inexpensive Protein Production by High-Cell-Density Fermentation

(2011) Marian Wenzel et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Combined Effect of Improved Cell Yield and Increased Specific Productivity Enhances Recombinant Enzyme Production in Genome-Reduced Bacillus subtilis Strain MGB874

(2011) Kenji Manabe et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Functional Implementation of the Posttranslational SecB-SecA Protein-Targeting Pathway in Bacillus subtilis

(2011) Liuyang Diao et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enhancing Isoprene Production by Genetic Modification of the 1-Deoxy-d-Xylulose-5-Phosphate Pathway inBacillus subtilis

(2011) Junfeng Xue et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression

(2011) Shanshan Li et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Production of nattokinase by high cell density fed-batch culture of Bacillus subtilis

(2011) Eun-Yeong Kwon et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

De novo engineering and metabolic flux analysis of inosine biosynthesis in Bacillus subtilis

(2011) Haojian Li et al.   BIOTECHNOLOGY LETTERS

A new method for multiple gene inactivations in Bacillus subtilis 168, producing a strain free of selectable markers

(2011) Chong Zhang et al.   CANADIAN JOURNAL OF MICROBIOLOGY

Regulation and control of metabolic fluxes in microbes

(2011) Luca Gerosa et al.   CURRENT OPINION IN BIOTECHNOLOGY

High yield and cost-effective production of poly(γ-glutamic acid) with Bacillus subtilis

(2011) Jin Huang et al.   ENGINEERING IN LIFE SCIENCES

A systems-level approach for metabolic engineering of yeast cell factories

(2011) Il-Kwon Kim et al.   FEMS YEAST RESEARCH

Genome sequence of B. amyloliquefaciens type strain DSM7T reveals differences to plant-associated B. amyloliquefaciens FZB42

(2011) Christian Rückert et al.   JOURNAL OF BIOTECHNOLOGY

Development of a strong intracellular expression system for Bacillus subtilis by optimizing promoter elements

(2011) Trang Thi Phuong Phan et al.   JOURNAL OF BIOTECHNOLOGY

Isolation, Purification, and Enzymatic Characterization of Extracellular Chitosanase from Marine Bacterium Bacillus subtilis CH2

(2011) Chulhong Oh   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

One-step production of lactate from cellulose as the sole carbon source without any other organic nutrient by recombinant cellulolytic Bacillus subtilis

(2011) Xiao-Zhou Zhang et al.   METABOLIC ENGINEERING

Regulation of mtl operon promoter of Bacillus subtilis: requirements of its use in expression vectors

(2011) Kambiz Morabbi Heravi et al.   Microbial Cell Factories

Engineering microbial biofuel tolerance and export using efflux pumps

(2011) M. J. Dunlop et al.   Molecular Systems Biology

SubtiWiki--a comprehensive community resource for the model organism Bacillus subtilis

(2011) U. Mader et al.   NUCLEIC ACIDS RESEARCH

Production and structural characterization of surfactin (C14/Leu7) produced by Bacillus subtilis isolate LSFM-05 grown on raw glycerol from the biodiesel industry

(2011) Andreia Fonseca de Faria et al.   PROCESS BIOCHEMISTRY

Enhanced production of α-amylase fromBacillus subtilissubsp.spizizeniiin solid state fermentation by response surface methodology and its evaluation in the hydrolysis of raw potato starch

(2011) Sanjeev Kumar Soni et al.   STARCH-STARKE

Scale up and Application of Biosurfactant from Bacillus subtilis in Enhanced Oil Recovery

(2010) Hossein Amani et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Improved poly-γ-glutamic acid production by chromosomal integration of the Vitreoscilla hemoglobin gene (vgb) in Bacillus subtilis

(2010) Yishan Su et al.   BIORESOURCE TECHNOLOGY

The statistically optimized production of poly(γ-glutamic acid) by batch fermentation of a newly isolated Bacillus subtilis RKY3

(2010) Jae-Hoon Jeong et al.   BIORESOURCE TECHNOLOGY

Enhancement of riboflavin production with Bacillus subtilis by expression and site-directed mutagenesis of zwf and gnd gene from Corynebacterium glutamicum

(2010) Zhiwen Wang et al.   BIORESOURCE TECHNOLOGY

One-step production of biocommodities from lignocellulosic biomass by recombinant cellulolytic Bacillus subtilis: Opportunities and challenges

(2010) Xiao-Zhou Zhang et al.   ENGINEERING IN LIFE SCIENCES

Construction of Chromosomally Located T7 Expression System for Production of Heterologous Secreted Proteins in Bacillus subtilis

(2010) Po Ting Chen et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Purification and characterization of highly thermostable alkaline phosphatase produced from B. licheniformis MTCC 1483

(2010) R.M. Banik et al.   JOURNAL OF BIOTECHNOLOGY

Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis

(2010) Su-Jin Lee et al.   JOURNAL OF BIOTECHNOLOGY

Chicken feathers: a complex substrate for the co-production of α-amylase and proteases by B. licheniformis NH1

(2010) Noomen Hmidet et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Overexpression and characterization in Bacillus subtilis of a positionally nonspecific lipase from Proteus vulgaris

(2010) Yaping Lu et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Optimization of medium components for D-ribose production by transketolase-deficient Bacillus subtilis NJT-1507

(2010) Ting Fang et al.   KOREAN JOURNAL OF CHEMICAL ENGINEERING

Development of a Bacillus subtilis expression system using the improved Pglv promoter

(2010) Yang M Ming et al.   Microbial Cell Factories

Enhanced Extracellular Production of Heterologous Proteins in Bacillus subtilis by Deleting the C-terminal Region of the SecA Secretory Machinery

(2010) Hiroshi Kakeshtia et al.   MOLECULAR BIOTECHNOLOGY

Production of nattokinase by batch and fed-batch culture of Bacillus subtilis

(2010) Young-Han Cho et al.   New Biotechnology

Biosurfactants production by immobilized cells of Bacillus subtilis ATCC 21332 and their recovery by pertraction

(2010) Omar Chtioui et al.   PROCESS BIOCHEMISTRY

Manufacturing Molecules Through Metabolic Engineering

(2010) J. D. Keasling   SCIENCE

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

Simple, fast and high-efficiency transformation system for directed evolution of cellulase in Bacillus subtilis

(2010) Xiao-Zhou Zhang et al.   Microbial Biotechnology

Expression of thermostable α-amylase gene fromBacillus stearothermophilus in variousBacillus subtilis strains

(2009) Bahri Devrim Özcan et al.   ANNALS OF MICROBIOLOGY

Optimization of technical conditions of producing ribavirin byBacillus subtilis

(2009) Ning Chen et al.   ANNALS OF MICROBIOLOGY

Evaluation of Cashew Apple Juice for Surfactin Production by Bacillus subtilis LAMI008

(2009) Maria Valderez Ponte Rocha et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

A simple method to introduce marker-free genetic modifications into the chromosome of naturally nontransformable Bacillus amyloliquefaciens strains

(2009) Natalia P. Zakataeva et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Overexpression of glucose-6-phosphate dehydrogenase enhances riboflavin production in Bacillus subtilis

(2009) Yun Xia Duan et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Increased production of riboflavin by metabolic engineering of the purine pathway in Bacillus subtilis

(2009) Shuobo Shi et al.   BIOCHEMICAL ENGINEERING JOURNAL

Expression of thepgsBEncoding the Poly-gamma-DL-glutamate Synthetase ofBacillus subtilis (natto)

(2009) Keitarou KIMURA et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Industrial scale of optimization for the production of carboxymethylcellulase from rice bran by a marine bacterium, Bacillus subtilis subsp. subtilis A-53

(2009) Bo-Hwa Lee et al.   ENZYME AND MICROBIAL TECHNOLOGY

A Comprehensive Proteomics and Transcriptomics Analysis of Bacillus subtilis Salt Stress Adaptation

(2009) H. Hahne et al.   JOURNAL OF BACTERIOLOGY

Optimization of agro-residual medium for α-amylase production from a hyper-producingBacillus subtilisKCC103 in submerged fermentation

(2009) Gobinath Rajagopalan et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Enrichment of glutaminase production byBacillus subtilisRSP-GLU in submerged cultivation based on neural network—genetic algorithm approach

(2009) Thadikamala Sathish et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Transcriptome analysis guided metabolic engineering of Bacillus subtilis for riboflavin production

(2009) Shuobo Shi et al.   METABOLIC ENGINEERING

Homolactic fermentation from glucose and cellobiose using Bacillus subtilis

(2009) Susana Romero-Garcia et al.   Microbial Cell Factories

Connecting parts with processes: SubtiWiki and SubtiPathways integrate gene and pathway annotation for Bacillus subtilis

(2009) C. R. Lammers et al.   MICROBIOLOGY-SGM

From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later

(2009) V. Barbe et al.   MICROBIOLOGY-SGM

Synthetic protein scaffolds provide modular control over metabolic flux

(2009) John E Dueber et al.   NATURE BIOTECHNOLOGY

Stabilized gene duplication enables long-term selection-free heterologous pathway expression

(2009) Keith E J Tyo et al.   NATURE BIOTECHNOLOGY

13C-based metabolic flux analysis

(2009) Nicola Zamboni et al.   Nature Protocols

An antifungal chitinase produced by Bacillus subtilis using chitin waste as a carbon source

(2009) Wen-Teish Chang et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

iBsu1103: a new genome-scale metabolic model of Bacillus subtilis based on SEED annotations

(2009) Christopher S Henry et al.   GENOME BIOLOGY

Cre/lox System and PCR-Based Genome Engineering in Bacillus subtilis

(2008) X. Yan et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Optimization of Surfactin Production by Bacillus subtilis Isolate BS5

(2008) A. Mohammad Abdel-Mawgoud et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Effects of Cultivation Conditions on the Production of γ-PGA with Bacillus subtilis ZJU-7

(2008) Jie Chen et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Improvement of poly(γ-glutamic acid) biosynthesis and redistribution of metabolic flux with the presence of different additives in Bacillus subtilis CGMCC 0833

(2008) Qun Wu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology

(2008) V. Deepak et al.   BIORESOURCE TECHNOLOGY

Over-production of a glycoside hydrolase family 50 β-agarase from Agarivorans sp. JA-1 in Bacillus subtilis and the whitening effect of its product

(2008) Dong-Geun Lee et al.   BIOTECHNOLOGY LETTERS

High-Level Secretion of a Chimeric Thermostable Lichenase from Bacillus subtilis by Screening of Site-Mutated Signal Peptides with Structural Alterations

(2008) Ling-lin Fu et al.   CURRENT MICROBIOLOGY

Enhanced Recombinant Protein Productivity by Genome Reduction in Bacillus subtilis

(2008) T. Morimoto et al.   DNA RESEARCH

Production of biosurfactant by Bacillus subtilis LB5a on a pilot scale using cassava wastewater as substrate

(2008) Francisco Fábio Cavalcante Barros et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Cloning of amidase gene from Rhodococcus erythropolis and expression by distinct promoters in Bacillus subtilis

(2008) Yang Yue et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Introduction of marker-free deletions in Bacillus subtilis using the AraR repressor and the ara promoter

(2008) S. Liu et al.   MICROBIOLOGY-SGM

Development and Application of a Novel Signal Peptide Probe Vector with PGA as Reporter in Bacillus subtilis WB700: Twenty-Four Tat Pathway Signal Peptides from Bacillus subtilis were Monitored

(2008) Fa-Ming Zhu et al.   MOLECULAR BIOTECHNOLOGY

Expression of pectate lyase A from Aspergillus nidulans in Bacillus subtilis

(2008) Qingxin Zhao et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

α-Amylase production from catabolite derepressed Bacillus subtilis KCC103 utilizing sugarcane bagasse hydrolysate

(2007) Gobinath Rajagopalan et al.   BIORESOURCE TECHNOLOGY