Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 34, Issue 7, Pages 789-793Publisher
SPRINGER
DOI: 10.1007/s00449-011-0527-x
Keywords
Nattokinase; Bacillus subtilis; Fed-batch culture; High cell density culture
Funding
- Ministry of Knowledge Economy (MKE)
- Korea Institute for Advancement in Technology (KIAT)
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Bacillus subtilis was cultivated to high cell density for nattokinase production by pH-stat fed-batch culture. A concentrated mixture solution of glucose and peptone was automatically added by acid-supplying pump when culture pH rose above high limit. Effect of the ratio of glucose to peptone in feeding solution was investigated on cell growth and nattokinase production by changing the ratio from 0.2 to 5 g glucose/g peptone. The highest cell concentration was 77 g/L when the ratio was 0.2 g glucose/g peptone. Cell concentration decreased with increasing the ratio of glucose to peptone in feeding solution, while the optimum condition existed for nattokinase production. The highest nattokinase activity was 14,500 unit/mL at a ratio of 0.33 g glucose/g peptone, which was 4.3 times higher than that in batch culture.
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