Design and synthesis of a minimal bacterial genome

Recombinase-mediated cassette exchange (RMCE) system for functional genomics studies in Mycoplasma mycoides

(2015) Vladimir N Noskov et al.   BIOLOGICAL PROCEDURES ONLINE

Bacterial genome reduction using the progressive clustering of deletions via yeast sexual cycling

(2015) Yo Suzuki et al.   GENOME RESEARCH

Galactofuranose inMycoplasma mycoidesis important for membrane integrity and conceals adhesins but does not contribute to serum resistance

(2015) Elise Schieck et al.   MOLECULAR MICROBIOLOGY

The essential role of SepF in mycobacterial division

(2015) Susanne Gola et al.   MOLECULAR MICROBIOLOGY

Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium

(2015) M. Lluch-Senar et al.   Molecular Systems Biology

TREC-IN: gene knock-in genetic tool for genomes cloned in yeast

(2015) Suchismita Chandran et al.   BMC GENOMICS

Progress Toward Synthetic Cells

(2014) J. Craig Blain et al.   Annual Review of Biochemistry

Systems Biology Perspectives on Minimal and Simpler Cells

(2014) J. C. Xavier et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

Bacillus subtilis and Escherichia coli essential genes and minimal cell factories after one decade of genome engineering

(2014) M. Juhas et al.   MICROBIOLOGY-SGM

Functional optimization of gene clusters by combinatorial design and assembly

(2014) Michael J Smanski et al.   NATURE BIOTECHNOLOGY

Excess Membrane Synthesis Drives a Primitive Mode of Cell Proliferation

(2013) Romain Mercier et al.   CELL

Direct transfer of whole genomes from bacteria to yeast

(2013) Bogumil J Karas et al.   NATURE METHODS

Structure-Function Features of a Mycoplasma Glycolipid Synthase Derived from Structural Data Integration, Molecular Simulations, and Mutational Analysis

(2013) Javier Romero-García et al.   PLoS One

Structural and genetic analyses reveal the protein SepF as a new membrane anchor for the Z ring

(2013) R. Duman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

Synthetic Generation of Influenza Vaccine Viruses for Rapid Response to Pandemics

(2013) P. R. Dormitzer et al.   Science Translational Medicine

A Whole-Cell Computational Model Predicts Phenotype from Genotype

(2012) Jonathan R. Karr et al.   CELL

Methods and applications for assembling large DNA constructs

(2012) Chuck Merryman et al.   METABOLIC ENGINEERING

Crucial Role for Membrane Fluidity in Proliferation of Primitive Cells

(2012) Romain Mercier et al.   Cell Reports

Single-step linker-based combinatorial assembly of promoter and gene cassettes for pathway engineering

(2010) Adi Ramon et al.   BIOTECHNOLOGY LETTERS

FtsZ-less cell division in archaea and bacteria

(2010) Rolf Bernander et al.   CURRENT OPINION IN MICROBIOLOGY

Cell division in a minimal bacterium in the absence of ftsZ

(2010) Maria Lluch-Senar et al.   MOLECULAR MICROBIOLOGY

Chemical synthesis of the mouse mitochondrial genome

(2010) Daniel G Gibson et al.   NATURE METHODS

Tandem repeat coupled with endonuclease cleavage (TREC): a seamless modification tool for genome engineering in yeast

(2010) Vladimir N. Noskov et al.   NUCLEIC ACIDS RESEARCH

Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

(2010) D. G. Gibson et al.   SCIENCE

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered in Yeast

(2009) C. Lartigue et al.   SCIENCE

Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae

(2008) Natalay Kouprina et al.   Nature Protocols

One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome

(2008) D. G. Gibson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome

(2008) D. G. Gibson et al.   SCIENCE