One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment

Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool

(2014) Carlotta Ronda et al.   BIOTECHNOLOGY AND BIOENGINEERING

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

(2014) Xuebing Wu et al.   NATURE BIOTECHNOLOGY

A guide to genome engineering with programmable nucleases

(2014) Hyongbum Kim et al.   NATURE REVIEWS GENETICS

High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs

(2014) Katarzyna Duda et al.   NUCLEIC ACIDS RESEARCH

Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector

(2014) Ami M. Kabadi et al.   NUCLEIC ACIDS RESEARCH

Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

(2014) Tetsushi Sakuma et al.   Scientific Reports

CHO microRNA engineering is growing up: Recent successes and future challenges

(2013) Vaibhav Jadhav et al.   BIOTECHNOLOGY ADVANCES

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system

(2013) Albert W Cheng et al.   CELL RESEARCH

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

(2013) S. W. Cho et al.   GENOME RESEARCH

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome

(2013) Nathan E Lewis et al.   NATURE BIOTECHNOLOGY

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

(2013) Vikram Pattanayak et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line

(2011) Xun Xu et al.   NATURE BIOTECHNOLOGY

Methods in mammalian cell line engineering: from random mutagenesis to sequence-specific approaches

(2010) Oliver Krämer et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering

(2010) Morten HH Nørholm   BMC BIOTECHNOLOGY

BAKandBAXdeletion using zinc-finger nucleases yields apoptosis-resistant CHO cells

(2009) Gregory J. Cost et al.   BIOTECHNOLOGY AND BIOENGINEERING