Cellular engineering for therapeutic protein production: product quality, host modification, and process improvement

Increasing pentose phosphate pathway flux enhances recombinant protein production in Pichia pastoris

(2016) Justyna Nocon et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

High-throughput screening and selection of mammalian cells for enhanced protein production

(2016) Joseph J. Priola et al.   Biotechnology Journal

From Escherich to the Escherichia coli genome

(2016) Guillaume Méric et al.   LANCET INFECTIOUS DISEASES

Fresh from the biotech pipeline—2015

(2016) Chris Morrison   NATURE BIOTECHNOLOGY

A step forward to improve recombinant protein production in Pichia pastoris : From specific growth rate effect on protein secretion to carbon-starving conditions as advanced strategy

(2016) Xavier Garcia-Ortega et al.   PROCESS BIOCHEMISTRY

High-throughput screening and selection of mammalian cells for enhanced protein production

(2016) Joseph J. Priola et al.   Biotechnology Journal

OmpA signal peptide leads to heterogenous secretion of B. subtilis chitosanase enzyme from E. coli expression system

(2016) Phornsiri Pechsrichuang et al.   SpringerPlus

The art of CHO cell engineering: A comprehensive retrospect and future perspectives

(2015) Simon Fischer et al.   BIOTECHNOLOGY ADVANCES

Engineering protein folding and translocation improves heterologous protein secretion inSaccharomyces cerevisiae

(2015) Hongting Tang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Glycoengineering of Chinese hamster ovary cells for enhanced erythropoietin N-glycan branching and sialylation

(2015) Bojiao Yin et al.   BIOTECHNOLOGY AND BIOENGINEERING

One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment

(2015) Lise Marie Grav et al.   Biotechnology Journal

Efforts to make and apply humanized yeast

(2015) Jon M. Laurent et al.   Briefings in Functional Genomics

Production of natural products through metabolic engineering of Saccharomyces cerevisiae

(2015) Anastasia Krivoruchko et al.   CURRENT OPINION IN BIOTECHNOLOGY

Engineering of oleaginous organisms for lipid production

(2015) Jennifer Levering et al.   CURRENT OPINION IN BIOTECHNOLOGY

Engineering yeast for producing human glycoproteins: where are we now?

(2015) Bram Laukens et al.   Future Microbiology

Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools

(2015) Sowmya Balasubramanian et al.   JOURNAL OF BIOTECHNOLOGY

Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast

(2015) Mingtao Huang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria

(2015) Michael-Paul Robinson et al.   Nature Communications

The development and characterization of synthetic minimal yeast promoters

(2015) Heidi Redden et al.   Nature Communications

One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment

(2015) Lise Marie Grav et al.   Biotechnology Journal

Impact of protein uptake and degradation on recombinant protein secretion in yeast

(2014) Keith E. J. Tyo et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Production of functional inclusion bodies in endotoxin-free Escherichia coli

(2014) Fabián Rueda et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Use of a small molecule cell cycle inhibitor to control cell growth and improve specific productivity and product quality of recombinant proteins in CHO cell cultures

(2014) Zhimei Du et al.   BIOTECHNOLOGY AND BIOENGINEERING

Blocking endocytotic mechanisms to improve heterologous protein titers inSaccharomyces cerevisiae

(2014) William A. Rodríguez-Limas et al.   BIOTECHNOLOGY AND BIOENGINEERING

Identification of manipulated variables for a glycosylation control strategy

(2014) Melissa M. St. Amand et al.   BIOTECHNOLOGY AND BIOENGINEERING

Recent developments in therapeutic protein expression technologies in plants

(2014) Shah Fahad et al.   BIOTECHNOLOGY LETTERS

Protein folding and secretion: mechanistic insights advancing recombinant protein production in S. cerevisiae

(2014) Carissa L Young et al.   CURRENT OPINION IN BIOTECHNOLOGY

Amino acid consumption in naïve and recombinant CHO cell cultures: producers of a monoclonal antibody

(2014) L. M. Carrillo-Cocom et al.   CYTOTECHNOLOGY

Over-expression of secreted proteins from mammalian cell lines

(2014) Annamarie C. Dalton et al.   PROTEIN SCIENCE

Design of synthetic yeast promoters via tuning of nucleosome architecture

(2014) Kathleen A. Curran et al.   Nature Communications

Fed-batch operational strategies for recombinant Fab production with Pichia pastoris using the constitutive GAP promoter

(2013) Xavier Garcia-Ortega et al.   BIOCHEMICAL ENGINEERING JOURNAL

Current state and recent advances in biopharmaceutical production in Escherichia coli, yeasts and mammalian cells

(2013) Aleš Berlec et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels

(2013) Susan Schlegel et al.   Microbial Cell Factories

Production of Recombinant Disulfide-Rich Venom Peptides for Structural and Functional Analysis via Expression in the Periplasm of E. coli

(2013) Julie K. Klint et al.   PLoS One

Comparison of Internal Ribosome Entry Site (IRES) and Furin-2A (F2A) for Monoclonal Antibody Expression Level and Quality in CHO Cells

(2013) Steven C. L. Ho et al.   PLoS One

A Practical Comparison of Ligation-Independent Cloning Techniques

(2013) Julian Stevenson et al.   PLoS One

An Internal Ribosome Entry Site (IRES) Mutant Library for Tuning Expression Level of Multiple Genes in Mammalian Cells

(2013) Esther Y. C. Koh et al.   PLoS One

Expanding the glycoengineering toolbox: the rise of bacterial N-linked protein glycosylation

(2013) Jenny L. Baker et al.   TRENDS IN BIOTECHNOLOGY

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

CHO Quasispecies—Implications for Manufacturing Processes

(2013) Florian Wurm   Processes

A Combined System for Engineering Glycosylation Efficiency and Glycan Structure in Saccharomyces cerevisiae

(2012) Farnoush Parsaie Nasab et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Effect of pH, Temperature, and Salt on the Stability of Escherichia coli- and Chinese Hamster Ovary Cell-Derived IgG1 Fc

(2012) Cynthia H. Li et al.   BIOCHEMISTRY

Industrial production of recombinant therapeutics in Escherichia coli and its recent advancements

(2012) Chung-Jr Huang et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

(2012) Julie Lobstein et al.   Microbial Cell Factories

Efficient extracellular secretion of an endoglucanase and a β-glucosidase in E. coli

(2012) Shefali Gupta et al.   PROTEIN EXPRESSION AND PURIFICATION

Periplasmic production via the pET expression system of soluble, bioactive human growth hormone

(2012) Jonathan T. Sockolosky et al.   PROTEIN EXPRESSION AND PURIFICATION

Different expression systems for production of recombinant proteins in Saccharomyces cerevisiae

(2011) Zihe Liu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Introduction to current and future protein therapeutics: A protein engineering perspective

(2011) Paul J. Carter   EXPERIMENTAL CELL RESEARCH

Production of Secretory and Extracellular N-Linked Glycoproteins inEscherichia coli

(2010) Adam C. Fisher et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering of protein secretion in yeast: strategies and impact on protein production

(2010) Alimjan Idiris et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Maximizing productivity of CHO cell-based fed-batch culture using chemically defined media conditions and typical manufacturing equipment

(2010) Yao-Ming Huang et al.   BIOTECHNOLOGY PROGRESS

Production of recombinant proteins by microbes and higher organisms

(2009) Arnold L. Demain et al.   BIOTECHNOLOGY ADVANCES

BAKandBAXdeletion using zinc-finger nucleases yields apoptosis-resistant CHO cells

(2009) Gregory J. Cost et al.   BIOTECHNOLOGY AND BIOENGINEERING

Microbial factories for recombinant pharmaceuticals

(2009) Neus Ferrer-Miralles et al.   Microbial Cell Factories

Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology

(2009) Pieter P Jacobs et al.   Nature Protocols

Increasing yeast secretion of heterologous proteins by regulating expression rates and post-secretory loss

(2008) Dagang Huang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Terminal sugars of Fc glycans influence antibody effector functions of IgGs

(2008) T Shantha Raju   CURRENT OPINION IN IMMUNOLOGY

Recent advances on the GAP promoter derived expression system of Pichia pastoris

(2008) Ai-Lian Zhang et al.   MOLECULAR BIOLOGY REPORTS

Heterologous GPCR Expression: A Bottleneck to Obtaining Crystal Structures

(2007) Emily C. McCusker et al.   BIOTECHNOLOGY PROGRESS

Protein therapeutics: a summary and pharmacological classification

(2007) Benjamin Leader et al.   NATURE REVIEWS DRUG DISCOVERY

On the Optimal Ratio of Heavy to Light Chain Genes for Efficient Recombinant Antibody Production by CHO Cells

(2005) Stefan Schlatter et al.   BIOTECHNOLOGY PROGRESS