Amino acid and glucose metabolism in fed-batch CHO cell culture affects antibody production and glycosylation

Differential Effect of Culture Temperature and Specific Growth Rate on CHO Cell Behavior in Chemostat Culture

(2014) Mauricio Vergara et al.   PLoS One

An optimized method for extraction and quantification of nucleotides and nucleotide sugars from mammalian cells

(2013) Ioscani Jimenez del Val et al.   ANALYTICAL BIOCHEMISTRY

Metabolic signatures of GS-CHO cell clones associated with butyrate treatment and culture phase transition

(2013) Nuno Carinhas et al.   BIOTECHNOLOGY AND BIOENGINEERING

Metabolic analysis of antibody producing CHO cells in fed-batch production

(2013) Jason Dean et al.   BIOTECHNOLOGY AND BIOENGINEERING

CHO cell line specific prediction and control of recombinant monoclonal antibodyN-glycosylation

(2013) Rhian K. Grainger et al.   BIOTECHNOLOGY AND BIOENGINEERING

Advances in improving mammalian cells metabolism for recombinant protein production

(2013) Claudia Altamirano et al.   ELECTRONIC JOURNAL OF BIOTECHNOLOGY

The availability of glucose to CHO cells affects the intracellular lipid-linked oligosaccharide distribution, site occupancy and the N-glycosylation profile of a monoclonal antibody

(2013) Bo Liu et al.   JOURNAL OF BIOTECHNOLOGY

Stable inhibition of mmu-miR-466h-5p improves apoptosis resistance and protein production in CHO cells

(2013) Aliaksandr Druz et al.   METABOLIC ENGINEERING

On metabolic shift to lactate consumption in fed-batch culture of mammalian cells

(2012) Bhanu Chandra Mulukutla et al.   METABOLIC ENGINEERING

Metabolite profiling of recombinant CHO cells: Designing tailored feeding regimes that enhance recombinant antibody production

(2011) Christopher A. Sellick et al.   BIOTECHNOLOGY AND BIOENGINEERING

Modulation of antibody galactosylation through feeding of uridine, manganese chloride, and galactose

(2011) Michael J. Gramer et al.   BIOTECHNOLOGY AND BIOENGINEERING

Feeding lactate for CHO cell culture processes: Impact on culture metabolism and performance

(2011) Jincai Li et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-End pH-controlled delivery of glucose effectively suppresses lactate accumulation in CHO Fed-batch cultures

(2011) Matthew Gagnon et al.   BIOTECHNOLOGY AND BIOENGINEERING

Effects of cell culture conditions on antibody N-linked glycosylation-what affects high mannose 5 glycoform

(2011) Efren Pacis et al.   BIOTECHNOLOGY AND BIOENGINEERING

Use of a Plackett-Burman statistical design to determine the effect of selected amino acids on monoclonal antibody production in CHO cells

(2011) I. J. González-Leal et al.   BIOTECHNOLOGY PROGRESS

Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinases

(2011) Meixia Zhou et al.   JOURNAL OF BIOTECHNOLOGY

Metabolic flux analysis of CHO cells at growth and non-growth phases using isotopic tracers and mass spectrometry

(2011) Woo Suk Ahn et al.   METABOLIC ENGINEERING

Profiling of N-glycosylation gene expression in CHO cell fed-batch cultures

(2010) Danny Chee Furng Wong et al.   BIOTECHNOLOGY AND BIOENGINEERING

An investigation of intracellular glycosylation activities in CHO cells: Effects of nucleotide sugar precursor feeding

(2010) Niki S.C. Wong et al.   BIOTECHNOLOGY AND BIOENGINEERING

Towards the implementation of quality by design to the production of therapeutic monoclonal antibodies with desired glycosylation patterns

(2010) Ioscani Jimenez del Val et al.   BIOTECHNOLOGY PROGRESS

Maximizing productivity of CHO cell-based fed-batch culture using chemically defined media conditions and typical manufacturing equipment

(2010) Yao-Ming Huang et al.   BIOTECHNOLOGY PROGRESS

Biopharmaceutical benchmarks 2010

(2010) Gary Walsh   NATURE BIOTECHNOLOGY

Expression of anti-apoptosis genes alters lactate metabolism of Chinese Hamster Ovary cells in culture

(2009) Haimanti Dorai et al.   BIOTECHNOLOGY AND BIOENGINEERING

Optimal and consistent protein glycosylation in mammalian cell culture

(2009) P. Hossler et al.   GLYCOBIOLOGY

Elevated Golgi pH impairs terminalN-glycosylation by inducing mislocalization of Golgi glycosyltransferases

(2009) Antti Rivinoja et al.   JOURNAL OF CELLULAR PHYSIOLOGY

Metabolic flux analysis in mammalian cell culture

(2009) Lake-Ee Quek et al.   METABOLIC ENGINEERING

Glycosylation as a strategy to improve antibody-based therapeutics

(2009) Roy Jefferis   NATURE REVIEWS DRUG DISCOVERY

Recombinant antibody therapeutics: the impact of glycosylation on mechanisms of action

(2009) Royston Jefferis   TRENDS IN PHARMACOLOGICAL SCIENCES

Influence of intracellular nucleotide and nucleotide sugar contents on recombinant interferon-γ glycosylation during batch and fed-batch cultures of CHO cells

(2008) N. Kochanowski et al.   BIOTECHNOLOGY AND BIOENGINEERING

Effect of culture temperature on erythropoietin production and glycosylation in a perfusion culture of recombinant CHO cells

(2008) Woo Suk Ahn et al.   BIOTECHNOLOGY AND BIOENGINEERING

Terminal sugars of Fc glycans influence antibody effector functions of IgGs

(2008) T Shantha Raju   CURRENT OPINION IN IMMUNOLOGY

Effect of Shear Stress on Intrinsic CHO Culture State and Glycosylation of Recombinant Tissue-Type Plasminogen Activator Protein

(2003) Ryan S. Senger et al.   BIOTECHNOLOGY PROGRESS